Reviewed, UniProtKB/Swiss-Prot Q91ZV4 (MOGT1_MOUSE)

Names and origin

Protein names

Recommended name:
    2-acylglycerol O-acyltransferase 1
    EC=2.3.1.22
Alternative name(s):
    Monoacylglycerol O-acyltransferase 1
    Acyl CoA:monoacylglycerol acyltransferase 1
      Short name=MGAT1
    Diacylglycerol acyltransferase 2-like protein 1

Gene names

Name:	Mogat1
Synonyms:	Dgat2l1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of diacylglycerol from 2-monoacylglycerol and fatty acyl-CoA. Probably not involved in absorption of dietary fat in the small intestine. Ref.4
Catalytic activity	Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol. Ref.4
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Probable Ref.4.
Tissue specificity	Expressed at high level in kidney and stomach. Expressed at lower level in brown and white adipose tissue, uterus and liver. Not detected in small intestine. Ref.4
Sequence similarities	Belongs to the diacylglycerol acyltransferase family.

Ontologies

Keywords
Biological process	Glycerol metabolism Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	diacylglycerol biosynthetic process Ref.4 Inferred from direct assay. Source: MGI glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Ref.4 Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Ref.4 Inferred from direct assay. Source: MGI
Molecular function	2-acylglycerol O-acyltransferase activity Ref.4 Inferred from direct assay. Source: MGI diacylglycerol O-acyltransferase activity Ref.4 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	2-acylglycerol O-acyltransferase 1		PRO_0000249059
Regions
	Transmembrane	24 – 44	21	Potential
	Transmembrane	104 – 124	21	Potential
Amino acid modifications
	Glycosylation	180	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	85 – 86	2	KE → FL in BAB22288. Ref.2
	Sequence conflict	294	1	I → T in BAB22288. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q91ZV4-1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: A27297B6DD51AD30	FASTA	335	38,803
	10 20 30 40 50 60 MMVEFAPLNT PLARCLQTAA VLQWVLSFLL LVQVCIGIMV MLVLYNYWFL YIPYLVWFYY 70 80 90 100 110 120 DWRTPEQGGR RWNWVQSWPV WKYFKEYFPI CLVKTQDLDP GHNYIFGFHP HGIFVPGAFG 130 140 150 160 170 180 NFCTKYSDFK KLFPGFTSYL HVAKIWFCFP LFREYLMSNG PVSVSKESLS HVLSKDGGGN 190 200 210 220 230 240 VSIIVLGGAK EALEAHPGTF TLCIRQRKGF VKMALTHGAS LVPVFSFGEN DLYKQINNPK 250 260 270 280 290 300 GSWLRTIQDA MYDSMGVALP LIYARGIFQH YFGIMPYRKL IYTVVGRPIP VQQILNPTSE 310 320 330 QIEELHQTYL EELKKLFNEH KGKYGIPEHE TLVFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members." Cases S., Stone S.J., Zhou P., Yen C.-L.E., Tow B., Lardizabal K.D., Voelker T., Farese R.V. Jr. J. Biol. Chem. 276:38870-38876(2001) [PubMed: 11481335] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Kidney.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Kidney.
[4]	"Identification of a gene encoding MGAT1, a monoacylglycerol acyltransferase." Yen C.-L.E., Stone S.J., Cases S., Zhou P., Farese R.V. Jr. Proc. Natl. Acad. Sci. U.S.A. 99:8512-8517(2002) [PubMed: 12077311] [Abstract] Cited for: CATALYTIC ACTIVITY, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF384162 mRNA. Translation: AAK84177.1. AK002693 mRNA. Translation: BAB22288.1. BC106135 mRNA. Translation: AAI06136.1.
IPI	IPI00121027.
RefSeq	NP_080989.2.
UniGene	Mm.41325
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q91ZV4.
Proteomic databases
PRIDE	Q91ZV4.
Genome annotation databases
Ensembl	ENSMUST00000012331; ENSMUSP00000012331; ENSMUSG00000012187; Mus musculus. [Genome view] ENSMUST00000113524; ENSMUSP00000109152; ENSMUSG00000012187; Mus musculus. [Genome view]
GeneID	68393.
KEGG	mmu:68393.
UCSC	uc007bqk.1. mouse.
Organism-specific databases
CTD	68393.
MGI	MGI:1915643. Mogat1.
Phylogenomic databases
HOGENOM	HBG561030.
HOVERGEN	Q91ZV4.
InParanoid	Q91ZV4.
PhylomeDB	Q91ZV4.
Enzyme and pathway databases
BRENDA	2.3.1.22. 244.
Gene expression databases
ArrayExpress	Q91ZV4.
Bgee	Q91ZV4.
CleanEx	MM_MOGAT1.
Genevestigator	Q91ZV4.
GermOnline	ENSMUSG00000012187. Mus musculus.
Family and domain databases
InterPro	IPR007130. DAGAT. [Graphical view]
PANTHER	PTHR12317. DAGAT. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	327104.
SOURCE	Search...

Entry information

Entry name

MOGT1_MOUSE

Accession

Primary (citable) accession number: Q91ZV4
Secondary accession number(s): Q9DCL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	December 1, 2001
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)