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Protein

Melanoma inhibitory activity protein 2

Gene

Mia2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. Isoform 1 plays a role in the secretion of lipoproteins, pre-chylomicrons and pre-VLDLs, by participating in their export from the endoplasmic reticulum (By similarity). Thereby, may play a role in cholesterol and triglyceride homoestasis (PubMed:21807889).By similarity1 Publication

GO - Biological processi

  • cargo loading into vesicle Source: UniProtKB
  • cholesterol homeostasis Source: MGI
  • ER to Golgi vesicle-mediated transport Source: UniProtKB
  • lipoprotein transport Source: UniProtKB
  • triglyceride homeostasis Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma inhibitory activity protein 2Curated
Gene namesi
Name:Mia2Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2159614. Mia2.

Subcellular locationi

Isoform 1 :
  • Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein 1 Publication

  • Note: Endoplasmic reticulum exit sites.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 604582LumenalCuratedAdd
BLAST
Intramembranei605 – 62521Sequence analysisAdd
BLAST
Topological domaini626 – 6283LumenalCurated
Transmembranei629 – 64921HelicalSequence analysisAdd
BLAST
Topological domaini650 – 1396747CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi90 – 901F → S: Decreases expression of isoform 1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 13961374Melanoma inhibitory activity protein 2Sequence analysisPRO_0000019032Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi59 – 591N-linked (GlcNAc...)Sequence analysis
Glycosylationi366 – 3661N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ91ZV0.
PeptideAtlasiQ91ZV0.
PRIDEiQ91ZV0.

PTM databases

PhosphoSiteiQ91ZV0.

Expressioni

Tissue specificityi

Isoform 1 is expressed in liver (at protein level) (PubMed:21807889). Isoform 2 is highly expressed in liver and weakly in testis (PubMed:12586826).2 Publications

Developmental stagei

Expression is seen at E12.5 and E14.5 embryonic stages.1 Publication

Gene expression databases

BgeeiENSMUSG00000035349.
CleanExiMM_MIA2.
GenevisibleiQ91ZV0. MM.

Interactioni

Subunit structurei

Isoform 1 may interact with APOB and MIA3 (By similarity). Isoform 1 may interact with the COPII coat subunits SEC23A and SEC24C (PubMed:21807889).By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ91ZV0.
SMRiQ91ZV0. Positions 27-117.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 10163SH3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1105 – 1396292Proline-rich domain (PRD); probably mediates interaction with COPII coat subunits1 PublicationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili693 – 867175Sequence analysisAdd
BLAST
Coiled coili914 – 1082169Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1269 – 1391123Pro-richPROSITE-ProRule annotationAdd
BLAST

Domaini

The proline-rich domain (PRD) probably mediates interaction with the COPII coat subunits.1 Publication

Sequence similaritiesi

Belongs to the MIA/OTOR family.Curated
Contains 1 SH3 domain.Curated

Keywords - Domaini

Coiled coil, SH3 domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIF2. Eukaryota.
ENOG41118PB. LUCA.
GeneTreeiENSGT00530000063635.
HOGENOMiHOG000220907.
HOVERGENiHBG049225.
InParanoidiQ91ZV0.
OMAiGDLECEX.
OrthoDBiEOG091G0PEA.
PhylomeDBiQ91ZV0.
TreeFamiTF332724.

Family and domain databases

InterProiIPR029782. MIA2.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23158:SF42. PTHR23158:SF42. 1 hit.
PfamiPF07653. SH3_2. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91ZV0-1) [UniParc]FASTAAdd to basket
Also known as: Mia2 p2401 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEVSVQRIL LLVVSLAKCL EGTKLLAHLK KCGDLECETL ISRVLALRDY
60 70 80 90 100
TGPDCRYLNF TTGEEISVYV KLGGDREDLW AGSKGKDFGF FPRDAVEIEE
110 120 130 140 150
VFISEEVEMP TKSDFLCLLG EGYIFGSEQS ELNSEDDEEH MYPYEKDEDQ
160 170 180 190 200
NYNIYEGDFQ PEPDLYAAAE GTLLEDQIPA SEAPDDFRFS SEWKAWEGAG
210 220 230 240 250
SQGGGEQDYT ADSDQDLPSL SKPERQGWFG LGTEEAEEKV FESDTEPTQE
260 270 280 290 300
LALEEESDLE KLHSGEPQVE LEQEPKSETL EFSSVPDEEY ELESETESIL
310 320 330 340 350
KPQASGWFGE GLTSYLGFGN EEAGLELLSK ESNPPLQDIP SSVPPDEEVP
360 370 380 390 400
APCREISTDK EDAVINDSSV LSPSWFYYGF GMLGFTNADE DNIVSDKGEN
410 420 430 440 450
EDGEVDNLKH PIGSDFDPEK EQERKIVTVE TEDQAGTESV LEKTDESGSM
460 470 480 490 500
QYLKKFFDNP WGFQSLPEDT ELPFSKKMLD QDDIVENDKI EELSTENSPT
510 520 530 540 550
GSMKDPVMLA SRYVLSDIDS EVELPMEEHE GVHFKPSSSK RNEDDSNSWA
560 570 580 590 600
DPEELSVAQT DGSAEGALLD TQLVSPKEHA ADFQLLKYLL QIDVYGFMSS
610 620 630 640 650
ALSPIEILLE SVVAALPEDM RADFNPSGFS LELAVCVLSV GLLAVVLFLW
660 670 680 690 700
RGFRSIRSRF YVGREKKLAL ELSALIEEKC KLLDKVSIVQ KEYEGLESSL
710 720 730 740 750
KEASFEKEST EAQSLEFVEG SQISEATYEN LEQSKSKLED EILLLEEKLE
760 770 780 790 800
EERAKHSEQD ELMADISKRI QSLEDESKSL KSQVAEAKTT FRIFEINEER
810 820 830 840 850
LKGAIKDALN ENSQLQESQK QLLQETEMMK EQVNDLDKQK VALEESRAQA
860 870 880 890 900
EQALSEKESQ IETLVTSLLK MKDWAAVLGE ADDGNLDLDM KSGLENTAAL
910 920 930 940 950
DNQPKGALKK LIYAAKLNAS LKALEGERNQ VYTQLSEVDQ VKEDLTEHIK
960 970 980 990 1000
SLESKQASLQ SEKTEFESES QKLQQKLKVI TELYQENEMK LHRKLTVEEN
1010 1020 1030 1040 1050
YRLEKEEKLS KVDEKISHAT EELETCRQRA KDLEEELERT IHSYQGQVIS
1060 1070 1080 1090 1100
HEKKAHDNWL AARTLERNLN DLRKENAHNR QKLTETEFKF ELLEKDPYAL
1110 1120 1130 1140 1150
DVPNTAFGRE HSPYGPSPLG RPPSETRAFL SPPTLLEGPL RLSPLLPGGG
1160 1170 1180 1190 1200
GRGSRGPENL LDHQMNTERG ESSYDRLSDA PRAPSDRSLS PPWEQDRRMT
1210 1220 1230 1240 1250
AHPPPGQPYS DPALQRQDRF YPNSGRLSGP AELRSYNMPS LDKVDGPVPS
1260 1270 1280 1290 1300
EMESSGNGTK DNLGNSNVPD SPIPAECEAA GRGFFPPPFP PVRDPLFPVD
1310 1320 1330 1340 1350
PRSQFMRRGP SFPPPPPGSI YAAPRDYFPP RDFPGPPLPP FPGRTVYAPR
1360 1370 1380 1390
GFPPYLPPRA GFFPPPPHPE SRSELPPDLI PPSKEPAADP PETQEA
Note: Readthrough transcript producing a functional fusion protein MIA2-CTAGE5 with similarity to MIA3.1 Publication
Length:1,396
Mass (Da):156,461
Last modified:September 7, 2016 - v3
Checksum:i152ED0302432C438
GO
Isoform 2 (identifier: Q91ZV0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     517-517: D → G
     518-1396: Missing.

Show »
Length:517
Mass (Da):57,777
Checksum:iDCD459082827A781
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei517 – 5171D → G in isoform 2. VSP_058474
Alternative sequencei518 – 1396879Missing in isoform 2. VSP_058475Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF390177 mRNA. Translation: AAL26992.2.
CCDSiCCDS25934.1.
RefSeqiNP_796295.1. NM_177321.2.
UniGeneiMm.213215.

Genome annotation databases

EnsembliENSMUST00000021384; ENSMUSP00000021384; ENSMUSG00000035349.
GeneIDi338320.
KEGGimmu:338320.
UCSCiuc007nqc.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF390177 mRNA. Translation: AAL26992.2.
CCDSiCCDS25934.1.
RefSeqiNP_796295.1. NM_177321.2.
UniGeneiMm.213215.

3D structure databases

ProteinModelPortaliQ91ZV0.
SMRiQ91ZV0. Positions 27-117.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ91ZV0.

Proteomic databases

PaxDbiQ91ZV0.
PeptideAtlasiQ91ZV0.
PRIDEiQ91ZV0.

Protocols and materials databases

DNASUi338320.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021384; ENSMUSP00000021384; ENSMUSG00000035349.
GeneIDi338320.
KEGGimmu:338320.
UCSCiuc007nqc.1. mouse.

Organism-specific databases

CTDi117153.
MGIiMGI:2159614. Mia2.

Phylogenomic databases

eggNOGiENOG410IIF2. Eukaryota.
ENOG41118PB. LUCA.
GeneTreeiENSGT00530000063635.
HOGENOMiHOG000220907.
HOVERGENiHBG049225.
InParanoidiQ91ZV0.
OMAiGDLECEX.
OrthoDBiEOG091G0PEA.
PhylomeDBiQ91ZV0.
TreeFamiTF332724.

Miscellaneous databases

PROiQ91ZV0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035349.
CleanExiMM_MIA2.
GenevisibleiQ91ZV0. MM.

Family and domain databases

InterProiIPR029782. MIA2.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR23158:SF42. PTHR23158:SF42. 1 hit.
PfamiPF07653. SH3_2. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIA2_MOUSE
AccessioniPrimary (citable) accession number: Q91ZV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: September 7, 2016
Last modified: September 7, 2016
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is unclear whether MIA2 and CTAGE5 constitute 2 distinct genes or a single one similar to MIA3.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.