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Protein

Glutamate receptor ionotropic, NMDA 3B

Gene

Grin3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NMDA receptor subtype of glutamate-gated ion channels with reduced single-channel conductance, low calcium permeability and low voltage-dependent sensitivity to magnesium. Mediated by glycine.

GO - Molecular functioni

GO - Biological processi

  • calcium ion transmembrane transport Source: GOC
  • protein insertion into membrane Source: UniProtKB
  • regulation of calcium ion transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Keywords - Ligandi

Calcium, Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor ionotropic, NMDA 3B
Short name:
GluN3B
Alternative name(s):
N-methyl-D-aspartate receptor subunit NR3B
Short name:
NMDAR3B
Short name:
NR3B
NMDA receptor 4
Short name:
Nr4
Gene namesi
Name:Grin3b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2150393. Grin3b.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
  • Cell junctionsynapsepostsynaptic cell membrane 1 Publication

  • Note: Requires the presence of GRIN1 to be targeted at the plasma membrane.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 564540ExtracellularSequence analysisAdd
BLAST
Transmembranei565 – 58521HelicalSequence analysisAdd
BLAST
Topological domaini586 – 64863CytoplasmicSequence analysisAdd
BLAST
Transmembranei649 – 66921HelicalSequence analysisAdd
BLAST
Topological domaini670 – 826157ExtracellularSequence analysisAdd
BLAST
Transmembranei827 – 84721HelicalSequence analysisAdd
BLAST
Topological domaini848 – 1003156CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • neuronal cell body Source: UniProtKB
  • NMDA selective glutamate receptor complex Source: UniProtKB
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 1003979Glutamate receptor ionotropic, NMDA 3BPRO_0000011571Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi69 – 691N-linked (GlcNAc...)Sequence analysis
Glycosylationi212 – 2121N-linked (GlcNAc...)Sequence analysis
Glycosylationi344 – 3441N-linked (GlcNAc...)Sequence analysis
Glycosylationi451 – 4511N-linked (GlcNAc...)Sequence analysis
Glycosylationi465 – 4651N-linked (GlcNAc...)Sequence analysis
Glycosylationi786 – 7861N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ91ZU9.
PRIDEiQ91ZU9.

PTM databases

PhosphoSiteiQ91ZU9.

Expressioni

Tissue specificityi

Expressed in the facial nucleus and the ambiguus nucleus of the brainstem, pons, medulla, spinal cord and cerebellum.3 Publications

Gene expression databases

BgeeiQ91ZU9.
CleanExiMM_GRIN3B.
ExpressionAtlasiQ91ZU9. baseline and differential.
GenevisibleiQ91ZU9. MM.

Interactioni

Subunit structurei

Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Does not form functional homomeric channels. Found in a complex with GRIN1 and GRIN2A or GRIN2B.2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048576.

Structurei

3D structure databases

ProteinModelPortaliQ91ZU9.
SMRiQ91ZU9. Positions 414-562, 676-808.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni952 – 98534Involved in the trafficking and surface expression of NMDARsAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili947 – 98640Sequence analysisAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1053. Eukaryota.
ENOG410XNUR. LUCA.
GeneTreeiENSGT00760000119186.
HOGENOMiHOG000231528.
HOVERGENiHBG052634.
InParanoidiQ91ZU9.
KOiK05214.
OMAiHLQLDWA.
OrthoDBiEOG7QK0B4.
PhylomeDBiQ91ZU9.
TreeFamiTF314731.

Family and domain databases

InterProiIPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
[Graphical view]
PfamiPF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91ZU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MECVQTLWLS LALALARGSW VVRGHPQPCG VPTRAGASVR LAALLPRAPA
60 70 80 90 100
ARARVLAALA TPSPRLPHNL SLELVAVASP TRDPASLARG LCQVLAPPGV
110 120 130 140 150
VASITFPEAR PELRLLQFLA AATETPVLSV LRREVRAPLG APTPFHLQLD
160 170 180 190 200
WASPLETILD VLVSLVRAHA WEDIALVLCR VRDPSGLVTL WTSRASQAPK
210 220 230 240 250
FVLDLSQLDS GNDSLRATLA LLGTLEGGGT PVSAAVLLGC STAHAHEVLE
260 270 280 290 300
AAPPGPQWLL GTPLPAEALP KTGLPPGVLV LGETGQPSLE AAVHDMVELV
310 320 330 340 350
ARALSSMALM HPERALLPAA VNCEDLKTGG SESTARTLAR FLSNTSFQGR
360 370 380 390 400
TGAVWVAGSS QVHVSRHFKV WSLRRDPLGA PAWATVGSWQ DGQLDFQPGA
410 420 430 440 450
AALRVPSPSG TQARPKLRVV TLVEHPFVFT RESDEDGQCP AGQLCLDPGT
460 470 480 490 500
NDSARLDALF TALENGSVPR TLRRCCYGYC IDLLERLAED LAFDFELYIV
510 520 530 540 550
GDGKYGALRD GRWTGLVGDL LAGRAHMAVT SFSINSARSQ VVDFTSPFFS
560 570 580 590 600
TSLGIMVRTR DTASPIGAFM WPLHWSMWVG VFAALHLTAL FLTLYEWRSP
610 620 630 640 650
YGLTPRGRNR GTVFSYSSAL NLCYAILFGR TVSSKTPKCP TGRFLMNLWA
660 670 680 690 700
IFCLLVLSSY TANLAAVMVG DKTFEELSGI HDPKLHHPSQ GFRFGTVWES
710 720 730 740 750
SAEAYIKASF PEMHAHMRRH SAPTTPHGVA MLTSDPPKLN AFIMDKSLLD
760 770 780 790 800
YEVSIDADCK LLTVGKPFAI EGYGIGLPQN SPLTSNLSEF ISRYKSSGFI
810 820 830 840 850
DLLHDKWYKM VPCGKRVFAV TETLQMGVYH LSGLFVLLCL GLGSALLTSL
860 870 880 890 900
GEHVFYRLVL PRIRRGNKLQ YWLHTSQKIH RALNTGPPEG QQERAEQECS
910 920 930 940 950
GPKEEQPAAD GAGRWRRVRR AVVERERRVR FLLEPGEAGG DHPWLCSNGP
960 970 980 990 1000
GVQAELRELE LRIEAARERL RSALLRRGEL RAQLGDGTRL RPLRLLHAAP

AES
Length:1,003
Mass (Da):109,158
Last modified:December 1, 2001 - v1
Checksum:i911CBF784E8B7A16
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti674 – 6741F → S in AAL40418 (PubMed:11735224).Curated
Sequence conflicti700 – 7012SS → GN in AAL40418 (PubMed:11735224).Curated
Sequence conflicti798 – 7981G → D in AAL40418 (PubMed:11735224).Curated
Sequence conflicti900 – 9001S → R in AAL40418 (PubMed:11735224).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF396649 mRNA. Translation: AAL10018.1.
AY062435 mRNA. Translation: AAL38983.1.
AF373861 mRNA. Translation: AAL40418.1.
CCDSiCCDS24001.1.
RefSeqiNP_569722.1. NM_130455.2.
UniGeneiMm.391566.

Genome annotation databases

EnsembliENSMUST00000045085; ENSMUSP00000048576; ENSMUSG00000035745.
GeneIDi170483.
KEGGimmu:170483.
UCSCiuc007gau.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF396649 mRNA. Translation: AAL10018.1.
AY062435 mRNA. Translation: AAL38983.1.
AF373861 mRNA. Translation: AAL40418.1.
CCDSiCCDS24001.1.
RefSeqiNP_569722.1. NM_130455.2.
UniGeneiMm.391566.

3D structure databases

ProteinModelPortaliQ91ZU9.
SMRiQ91ZU9. Positions 414-562, 676-808.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048576.

PTM databases

PhosphoSiteiQ91ZU9.

Proteomic databases

PaxDbiQ91ZU9.
PRIDEiQ91ZU9.

Protocols and materials databases

DNASUi170483.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045085; ENSMUSP00000048576; ENSMUSG00000035745.
GeneIDi170483.
KEGGimmu:170483.
UCSCiuc007gau.1. mouse.

Organism-specific databases

CTDi116444.
MGIiMGI:2150393. Grin3b.

Phylogenomic databases

eggNOGiKOG1053. Eukaryota.
ENOG410XNUR. LUCA.
GeneTreeiENSGT00760000119186.
HOGENOMiHOG000231528.
HOVERGENiHBG052634.
InParanoidiQ91ZU9.
KOiK05214.
OMAiHLQLDWA.
OrthoDBiEOG7QK0B4.
PhylomeDBiQ91ZU9.
TreeFamiTF314731.

Miscellaneous databases

NextBioi370111.
PROiQ91ZU9.
SOURCEiSearch...

Gene expression databases

BgeeiQ91ZU9.
CleanExiMM_GRIN3B.
ExpressionAtlasiQ91ZU9. baseline and differential.
GenevisibleiQ91ZU9. MM.

Family and domain databases

InterProiIPR019594. Glu/Gly-bd.
IPR001508. Iono_rcpt_met.
IPR001320. Iontro_rcpt.
[Graphical view]
PfamiPF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Motoneuron-specific expression of NR3B, a novel NMDA-type glutamate receptor subunit that works in a dominant-negative manner."
    Nishi M., Hinds H., Lu H.-P., Kawata M., Hayashi Y.
    J. Neurosci. 21:RC185.1-RC185.6(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, TISSUE SPECIFICITY.
    Tissue: Spinal cord.
  2. "Cloning and characterization of a novel NMDA receptor subunit NR3B: a dominant subunit that reduces calcium permeability."
    Matsuda K., Kamiya Y., Matsuda S., Yuzaki M.
    Brain Res. Mol. Brain Res. 100:43-52(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, TISSUE SPECIFICITY, IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN2A OR GRIN2B.
    Strain: ICR.
    Tissue: Cerebellum.
  3. "Nucleotide sequence, genomic organization, and chromosomal localization of genes encoding the human NMDA receptor subunits NR3A and NR3B."
    Andersson O., Stenqvist A., Attersand A., von Euler G.
    Genomics 78:178-184(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 360-900.
    Strain: BALB/cJ.
    Tissue: Brain.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 513-538 AND 631-643, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  5. "Specific assembly with the NMDA receptor 3B subunit controls surface expression and calcium permeability of NMDA receptors."
    Matsuda K., Fletcher M., Kamiya Y., Yuzaki M.
    J. Neurosci. 23:10064-10073(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH GRIN1 AND GRIN2A OR GRIN2B.

Entry informationi

Entry nameiNMD3B_MOUSE
AccessioniPrimary (citable) accession number: Q91ZU9
Secondary accession number(s): Q8VHV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.