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Protein

FYVE, RhoGEF and PH domain-containing protein 4

Gene

Fgd4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Activates MAPK8 (By similarity). Plays a role in regulating the actin cytoskeleton and cell shape. Promotes the formation of lamellipodia.By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri559 – 61961FYVE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • activation of JUN kinase activity Source: MGI
  • lamellipodium assembly Source: MGI
  • microspike assembly Source: MGI
  • regulation of Rho protein signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Ligandi

Actin-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-193648. NRAGE signals death through JNK.
R-MMU-194840. Rho GTPase cycle.
R-MMU-416482. G alpha (12/13) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
FYVE, RhoGEF and PH domain-containing protein 4
Alternative name(s):
Actin filament-binding protein frabin
FGD1-related F-actin-binding protein
Gene namesi
Name:Fgd4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2183747. Fgd4.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • cytoplasm Source: UniProtKB-KW
  • filopodium Source: MGI
  • lamellipodium Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 766766FYVE, RhoGEF and PH domain-containing protein 4PRO_0000080948Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei702 – 7021PhosphoserineBy similarity
Modified residuei716 – 7161PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ91ZT5.
PaxDbiQ91ZT5.
PeptideAtlasiQ91ZT5.
PRIDEiQ91ZT5.

PTM databases

iPTMnetiQ91ZT5.
PhosphoSiteiQ91ZT5.

Expressioni

Tissue specificityi

Detected in thymus, lung, heart, skeletal muscle, small intestine, liver, kidney, spleen and testis. Expressed in all parts of the brain and in the spinal cord at embryonic, postnatal, and adult stages. Levels of expression are lower in postnatal and adult tissues than in embryonic tissues.2 Publications

Gene expression databases

BgeeiENSMUSG00000022788.
CleanExiMM_FGD4.
ExpressionAtlasiQ91ZT5. baseline and differential.
GenevisibleiQ91ZT5. MM.

Interactioni

Subunit structurei

Homooligomer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ91ZT5. 2 interactions.
STRINGi10090.ENSMUSP00000125174.

Structurei

3D structure databases

ProteinModelPortaliQ91ZT5.
SMRiQ91ZT5. Positions 210-522, 549-619, 646-738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini206 – 393188DHPROSITE-ProRule annotationAdd
BLAST
Domaini422 – 521100PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini643 – 74098PH 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 150150Actin filament-bindingBy similarityAdd
BLAST

Domaini

The part of the protein spanning the actin filament-binding domain together with the DH domain and the first PH domain is necessary and sufficient for microspike formation. Activation of MAPK8 requires the presence of all domains with the exception of the actin filament-binding domain (By similarity).By similarity

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation
Contains 2 PH domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri559 – 61961FYVE-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG4424. Eukaryota.
ENOG410XRXV. LUCA.
GeneTreeiENSGT00750000117280.
HOVERGENiHBG007506.
InParanoidiQ91ZT5.
KOiK05723.
OMAiQTCVANG.
OrthoDBiEOG091G03FU.
PhylomeDBiQ91ZT5.
TreeFamiTF316247.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000219. DH-domain.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF00169. PH. 2 hits.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50729. SSF50729. 2 hits.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91ZT5-1) [UniParc]FASTAAdd to basket
Also known as: Frabin alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEESNPAPTS CTSKGKHSKV SDLISHFEGG SVLSSYIDLQ KDSTMNLNIP
60 70 80 90 100
QTLGQPGLTS SPPRKFLPQH SPQKQENDPD QTQGQHGCLA NGVVAAQNQM
110 120 130 140 150
ECEDEKETTL SPEMAIQTAA ASPDTHVLNG ERNETITDSA SSIANSHDEN
160 170 180 190 200
ASDSSCRTPG TDLGLPSKEG EPGMDAELQE RENGVNTMGL DTLDQHHEVK
210 220 230 240 250
ETNEQKLHKI ATELLLTERA YVSRLDLLDQ VFYCKLLEEA NRGSFPAEMV
260 270 280 290 300
NKIFSNISSI NAFHSKFLLP ELEKRMQEWE TTPRIGDILQ KLAPFLKMYG
310 320 330 340 350
EYVKGFDNAV ELVKTMTERV PQFKSVTEEI QKQKICGSLT LQHHMLEPIQ
360 370 380 390 400
RIPRYEMLLK DYLKKLSPDS PDWNDAKKSL EIISTAASHS NSAIRKMENL
410 420 430 440 450
KKLLEIYEML GEEEDIVNPS NELIKEGQIL KLAARNTSAQ ERYLFLFNNM
460 470 480 490 500
LLYCVPRFSL VGSKFTVRTR VGIDGMKIVE THNEEYPHTF QISGKERTLE
510 520 530 540 550
LQASSEQDKE EWIKALQESI DAFHQRHETF RNAIAKENDI PLEVSTAELG
560 570 580 590 600
KRAPRWIRDN EVTMCMKCKE SFNALTRRRH HCRACGHVVC WKCSDYKAQL
610 620 630 640 650
EYDGGRLNKV CKDCYQIISG FTDSEEKKRR GILEIESAEV SGNSEVCSFL
660 670 680 690 700
QYMEKSKPWQ KIWCVIPKQD PLVLYMYGAP QDVRAQATIP LLGYVVDDMP
710 720 730 740 750
KSADLPHSFK LTQSKSVHSF AADNEELKQK WLKIILLAVT GETPDGPSEH
760
LATLNNLPGP KKKSEC
Length:766
Mass (Da):86,541
Last modified:December 1, 2001 - v1
Checksum:i06BC1C01C6FCA274
GO
Isoform 2 (identifier: Q91ZT5-2) [UniParc]FASTAAdd to basket
Also known as: Frabin beta

The sequence of this isoform differs from the canonical sequence as follows:
     589-603: VCWKCSDYKAQLEYD → SEASSLSQLLEMVYR
     604-766: Missing.

Show »
Length:603
Mass (Da):68,210
Checksum:i645ED49AD97DC901
GO
Isoform 3 (identifier: Q91ZT5-3) [UniParc]FASTAAdd to basket
Also known as: Frabin gamma

The sequence of this isoform differs from the canonical sequence as follows:
     504-504: S → R
     505-766: Missing.

Show »
Length:504
Mass (Da):56,697
Checksum:i107EE4B4ECB43CE5
GO
Isoform 4 (identifier: Q91ZT5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-44: Missing.
     504-504: S → R
     505-766: Missing.

Note: No experimental confirmation available.
Show »
Length:460
Mass (Da):52,016
Checksum:iC1CF8B468DBA4718
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti36 – 361Y → C in BAC35703 (PubMed:16141072).Curated
Sequence conflicti49 – 491I → V in BAC35703 (PubMed:16141072).Curated
Sequence conflicti278 – 2781E → K in BAE28995 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4444Missing in isoform 4. 1 PublicationVSP_013083Add
BLAST
Alternative sequencei504 – 5041S → R in isoform 3 and isoform 4. 2 PublicationsVSP_013084
Alternative sequencei505 – 766262Missing in isoform 3 and isoform 4. 2 PublicationsVSP_013085Add
BLAST
Alternative sequencei589 – 60315VCWKC…QLEYD → SEASSLSQLLEMVYR in isoform 2. 1 PublicationVSP_013086Add
BLAST
Alternative sequencei604 – 766163Missing in isoform 2. 1 PublicationVSP_013087Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF402611 mRNA. Translation: AAL05631.1.
AF402612 mRNA. Translation: AAL05632.1.
AF402613 mRNA. Translation: AAL05633.1.
AK034504 mRNA. Translation: BAC28733.1.
AK054242 mRNA. Translation: BAC35703.2.
AK149626 mRNA. Translation: BAE28995.1.
BC129902 mRNA. Translation: AAI29903.1.
BC129903 mRNA. Translation: AAI29904.1.
CCDSiCCDS27986.1. [Q91ZT5-1]
RefSeqiNP_001288747.1. NM_001301818.1. [Q91ZT5-1]
NP_631978.1. NM_139232.3. [Q91ZT5-1]
XP_006522090.1. XM_006522027.2. [Q91ZT5-1]
XP_006522092.1. XM_006522029.1. [Q91ZT5-1]
UniGeneiMm.256131.

Genome annotation databases

EnsembliENSMUST00000161188; ENSMUSP00000123763; ENSMUSG00000022788. [Q91ZT5-2]
ENSMUST00000161861; ENSMUSP00000125174; ENSMUSG00000022788. [Q91ZT5-1]
ENSMUST00000162671; ENSMUSP00000125736; ENSMUSG00000022788. [Q91ZT5-1]
GeneIDi224014.
KEGGimmu:224014.
UCSCiuc007yip.2. mouse. [Q91ZT5-1]
uc007yis.1. mouse. [Q91ZT5-2]
uc007yit.3. mouse. [Q91ZT5-3]
uc007yiu.3. mouse. [Q91ZT5-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF402611 mRNA. Translation: AAL05631.1.
AF402612 mRNA. Translation: AAL05632.1.
AF402613 mRNA. Translation: AAL05633.1.
AK034504 mRNA. Translation: BAC28733.1.
AK054242 mRNA. Translation: BAC35703.2.
AK149626 mRNA. Translation: BAE28995.1.
BC129902 mRNA. Translation: AAI29903.1.
BC129903 mRNA. Translation: AAI29904.1.
CCDSiCCDS27986.1. [Q91ZT5-1]
RefSeqiNP_001288747.1. NM_001301818.1. [Q91ZT5-1]
NP_631978.1. NM_139232.3. [Q91ZT5-1]
XP_006522090.1. XM_006522027.2. [Q91ZT5-1]
XP_006522092.1. XM_006522029.1. [Q91ZT5-1]
UniGeneiMm.256131.

3D structure databases

ProteinModelPortaliQ91ZT5.
SMRiQ91ZT5. Positions 210-522, 549-619, 646-738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91ZT5. 2 interactions.
STRINGi10090.ENSMUSP00000125174.

PTM databases

iPTMnetiQ91ZT5.
PhosphoSiteiQ91ZT5.

Proteomic databases

MaxQBiQ91ZT5.
PaxDbiQ91ZT5.
PeptideAtlasiQ91ZT5.
PRIDEiQ91ZT5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000161188; ENSMUSP00000123763; ENSMUSG00000022788. [Q91ZT5-2]
ENSMUST00000161861; ENSMUSP00000125174; ENSMUSG00000022788. [Q91ZT5-1]
ENSMUST00000162671; ENSMUSP00000125736; ENSMUSG00000022788. [Q91ZT5-1]
GeneIDi224014.
KEGGimmu:224014.
UCSCiuc007yip.2. mouse. [Q91ZT5-1]
uc007yis.1. mouse. [Q91ZT5-2]
uc007yit.3. mouse. [Q91ZT5-3]
uc007yiu.3. mouse. [Q91ZT5-4]

Organism-specific databases

CTDi121512.
MGIiMGI:2183747. Fgd4.

Phylogenomic databases

eggNOGiKOG4424. Eukaryota.
ENOG410XRXV. LUCA.
GeneTreeiENSGT00750000117280.
HOVERGENiHBG007506.
InParanoidiQ91ZT5.
KOiK05723.
OMAiQTCVANG.
OrthoDBiEOG091G03FU.
PhylomeDBiQ91ZT5.
TreeFamiTF316247.

Enzyme and pathway databases

ReactomeiR-MMU-193648. NRAGE signals death through JNK.
R-MMU-194840. Rho GTPase cycle.
R-MMU-416482. G alpha (12/13) signalling events.

Miscellaneous databases

PROiQ91ZT5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022788.
CleanExiMM_FGD4.
ExpressionAtlasiQ91ZT5. baseline and differential.
GenevisibleiQ91ZT5. MM.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR000219. DH-domain.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
PF00169. PH. 2 hits.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
SM00233. PH. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50729. SSF50729. 2 hits.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFGD4_MOUSE
AccessioniPrimary (citable) accession number: Q91ZT5
Secondary accession number(s): A1L355
, Q3UEB6, Q8BW60, Q8BZI7, Q91ZT3, Q91ZT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.