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Protein

45 kDa calcium-binding protein

Gene

Sdf4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

A membrane-associated isoform may be involved in the exocytosis of zymogens by pancreatic acini. May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi110 – 121121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi149 – 160122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi208 – 219123PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi245 – 256124PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi290 – 301125PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi326 – 337126PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: BHF-UCL
  • identical protein binding Source: BHF-UCL

GO - Biological processi

  • calcium ion regulated exocytosis Source: BHF-UCL
  • cerebellum development Source: BHF-UCL
  • exocytosis Source: UniProtKB-KW
  • fat cell differentiation Source: BHF-UCL
  • response to ethanol Source: BHF-UCL
  • UV protection Source: BHF-UCL
  • zymogen granule exocytosis Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Exocytosis

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
45 kDa calcium-binding protein
Short name:
Cab45
Alternative name(s):
Stromal cell-derived factor 4
Short name:
SDF-4
Gene namesi
Name:Sdf4
Synonyms:Cab45
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621521. Sdf4.

Subcellular locationi

  • Golgi apparatus lumen By similarity

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • Golgi lumen Source: BHF-UCL
  • late endosome Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence analysisAdd
BLAST
Chaini36 – 36132645 kDa calcium-binding proteinPRO_0000004158Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi39 – 391N-linked (GlcNAc...)Sequence analysis
Modified residuei98 – 981PhosphoserineBy similarity
Modified residuei192 – 1921PhosphothreonineCombined sources
Modified residuei216 – 2161PhosphothreonineBy similarity
Modified residuei264 – 2641PhosphothreonineBy similarity
Modified residuei298 – 2981PhosphothreonineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ91ZS3.
PRIDEiQ91ZS3.

PTM databases

iPTMnetiQ91ZS3.
PhosphoSiteiQ91ZS3.

Expressioni

Tissue specificityi

A membrane-associated isoform is expressed in acini of the pancreas (at protein level). Ubiquitous.2 Publications

Inductioni

Down-regulated by ethanol. Down-regulated during the progression of cerebellum differentiation.1 Publication

Gene expression databases

BgeeiENSRNOG00000019981.

Interactioni

Subunit structurei

A membrane-associated isoform interacts with STX3 and STXBP1.1 Publication

GO - Molecular functioni

  • identical protein binding Source: BHF-UCL

Protein-protein interaction databases

MINTiMINT-4570260.
STRINGi10116.ENSRNOP00000027215.

Structurei

3D structure databases

ProteinModelPortaliQ91ZS3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini97 – 13236EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini136 – 17136EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini196 – 23136EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini232 – 26736EF-hand 4PROSITE-ProRule annotationAdd
BLAST
Domaini277 – 31236EF-hand 5PROSITE-ProRule annotationAdd
BLAST
Domaini313 – 34836EF-hand 6PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni308 – 36154Necessary for intracellular retention in Golgi apparatus lumenBy similarityAdd
BLAST

Domaini

Binds calcium via its EF-hands.By similarity

Sequence similaritiesi

Belongs to the CREC family.Curated
Contains 6 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG4251. Eukaryota.
ENOG410XSH7. LUCA.
HOGENOMiHOG000007329.
HOVERGENiHBG097344.
InParanoidiQ91ZS3.
KOiK19934.
PhylomeDBiQ91ZS3.
TreeFamiTF314849.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR027240. CAB45.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10827:SF51. PTHR10827:SF51. 1 hit.
PfamiPF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 5 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 5 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q91ZS3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVWLVAMTSR QRSLCGLAAH GLWFLGLVLL MDATARPANH SFSRERAANR
60 70 80 90 100
DENEIIPPDH LNGVKLEMDG HLNKDFHQEV FLGKDMDGFD EDSEPRRSRR
110 120 130 140 150
KLMVIFSKVD VNTDRRISAK EMQHWIMEKT AEHFQEAVKE NKLHFRAVDP
160 170 180 190 200
DGDGHVSWDE YKVKFLASKG HNEREIADAI KNHEELKVDE ETQEVLGNLR
210 220 230 240 250
DRWYQADNPP ADLLLTEDEF LSFLHPEHSR GMLKFMVKEI VRDLDQDGDK
260 270 280 290 300
QLSLPEFISL PVGTVENQQG QDIDDNWVKD RKKEFEELID SNHDGIVTME
310 320 330 340 350
ELENYMDPMN EYNALNEAKQ MIAIADENQN HHLEPEEILK YSEFFTGSKL
360
MDYARNVHEE F
Length:361
Mass (Da):42,075
Last modified:December 1, 2001 - v1
Checksum:i1BF74F014B033FC5
GO
Isoform 2 (identifier: Q91ZS3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTSRAPNCAPQTRRIRKPGSPV

Show »
Length:382
Mass (Da):44,350
Checksum:i0D75D4C8CF2C7F9C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51V → A in AAH86996 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 11M → MTSRAPNCAPQTRRIRKPGS PV in isoform 2. 1 PublicationVSP_037452

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF405545 mRNA. Translation: AAL01370.1.
BC086996 mRNA. Translation: AAH86996.1.
RefSeqiNP_569096.2. NM_130412.2.
UniGeneiRn.12964.

Genome annotation databases

GeneIDi155173.
KEGGirno:155173.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF405545 mRNA. Translation: AAL01370.1.
BC086996 mRNA. Translation: AAH86996.1.
RefSeqiNP_569096.2. NM_130412.2.
UniGeneiRn.12964.

3D structure databases

ProteinModelPortaliQ91ZS3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4570260.
STRINGi10116.ENSRNOP00000027215.

PTM databases

iPTMnetiQ91ZS3.
PhosphoSiteiQ91ZS3.

Proteomic databases

PaxDbiQ91ZS3.
PRIDEiQ91ZS3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi155173.
KEGGirno:155173.

Organism-specific databases

CTDi51150.
RGDi621521. Sdf4.

Phylogenomic databases

eggNOGiKOG4251. Eukaryota.
ENOG410XSH7. LUCA.
HOGENOMiHOG000007329.
HOVERGENiHBG097344.
InParanoidiQ91ZS3.
KOiK19934.
PhylomeDBiQ91ZS3.
TreeFamiTF314849.

Miscellaneous databases

PROiQ91ZS3.

Gene expression databases

BgeeiENSRNOG00000019981.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR027240. CAB45.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PANTHERiPTHR10827:SF51. PTHR10827:SF51. 1 hit.
PfamiPF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 5 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
PROSITEiPS00018. EF_HAND_1. 5 hits.
PS50222. EF_HAND_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAB45_RAT
AccessioniPrimary (citable) accession number: Q91ZS3
Secondary accession number(s): Q5PQW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.