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Protein

45 kDa calcium-binding protein

Gene

Sdf4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

A membrane-associated isoform may be involved in the exocytosis of zymogens by pancreatic acini. May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi110 – 1211PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi149 – 1602PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi208 – 2193PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi245 – 2564PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi290 – 3015PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi326 – 3376PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium ion binding Source: BHF-UCL
  • identical protein binding Source: BHF-UCL

GO - Biological processi

  • calcium ion regulated exocytosis Source: BHF-UCL
  • cerebellum development Source: BHF-UCL
  • exocytosis Source: UniProtKB-KW
  • fat cell differentiation Source: BHF-UCL
  • response to ethanol Source: BHF-UCL
  • UV protection Source: BHF-UCL
  • zymogen granule exocytosis Source: BHF-UCL

Keywordsi

Biological processExocytosis
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
45 kDa calcium-binding protein
Short name:
Cab45
Alternative name(s):
Stromal cell-derived factor 4
Short name:
SDF-4
Gene namesi
Name:Sdf4
Synonyms:Cab45
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621521 Sdf4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Sequence analysisAdd BLAST35
ChainiPRO_000000415836 – 36145 kDa calcium-binding proteinAdd BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi39N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei98PhosphoserineBy similarity1
Modified residuei192PhosphothreonineCombined sources1
Modified residuei216PhosphothreonineBy similarity1
Modified residuei264PhosphothreonineBy similarity1
Modified residuei298PhosphothreonineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ91ZS3
PRIDEiQ91ZS3

PTM databases

iPTMnetiQ91ZS3
PhosphoSitePlusiQ91ZS3

Expressioni

Tissue specificityi

A membrane-associated isoform is expressed in acini of the pancreas (at protein level). Ubiquitous.2 Publications

Inductioni

Down-regulated by ethanol. Down-regulated during the progression of cerebellum differentiation.1 Publication

Interactioni

Subunit structurei

A membrane-associated isoform interacts with STX3 and STXBP1.1 Publication

GO - Molecular functioni

  • identical protein binding Source: BHF-UCL

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027215

Structurei

3D structure databases

ProteinModelPortaliQ91ZS3
SMRiQ91ZS3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 132EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini136 – 171EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini196 – 231EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini232 – 267EF-hand 4PROSITE-ProRule annotationAdd BLAST36
Domaini277 – 312EF-hand 5PROSITE-ProRule annotationAdd BLAST36
Domaini313 – 348EF-hand 6PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni308 – 361Necessary for intracellular retention in Golgi apparatus lumenBy similarityAdd BLAST54

Domaini

Binds calcium via its EF-hands.By similarity

Sequence similaritiesi

Belongs to the CREC family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG4251 Eukaryota
ENOG410XSH7 LUCA
HOGENOMiHOG000007329
HOVERGENiHBG097344
InParanoidiQ91ZS3
KOiK19934
PhylomeDBiQ91ZS3
TreeFamiTF314849

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13202 EF-hand_5, 1 hit
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00054 EFh, 5 hits
SUPFAMiSSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 5 hits
PS50222 EF_HAND_2, 5 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q91ZS3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVWLVAMTSR QRSLCGLAAH GLWFLGLVLL MDATARPANH SFSRERAANR
60 70 80 90 100
DENEIIPPDH LNGVKLEMDG HLNKDFHQEV FLGKDMDGFD EDSEPRRSRR
110 120 130 140 150
KLMVIFSKVD VNTDRRISAK EMQHWIMEKT AEHFQEAVKE NKLHFRAVDP
160 170 180 190 200
DGDGHVSWDE YKVKFLASKG HNEREIADAI KNHEELKVDE ETQEVLGNLR
210 220 230 240 250
DRWYQADNPP ADLLLTEDEF LSFLHPEHSR GMLKFMVKEI VRDLDQDGDK
260 270 280 290 300
QLSLPEFISL PVGTVENQQG QDIDDNWVKD RKKEFEELID SNHDGIVTME
310 320 330 340 350
ELENYMDPMN EYNALNEAKQ MIAIADENQN HHLEPEEILK YSEFFTGSKL
360
MDYARNVHEE F
Length:361
Mass (Da):42,075
Last modified:December 1, 2001 - v1
Checksum:i1BF74F014B033FC5
GO
Isoform 2 (identifier: Q91ZS3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTSRAPNCAPQTRRIRKPGSPV

Show »
Length:382
Mass (Da):44,350
Checksum:i0D75D4C8CF2C7F9C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5V → A in AAH86996 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0374521M → MTSRAPNCAPQTRRIRKPGS PV in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF405545 mRNA Translation: AAL01370.1
BC086996 mRNA Translation: AAH86996.1
RefSeqiNP_569096.2, NM_130412.2
UniGeneiRn.12964

Genome annotation databases

GeneIDi155173
KEGGirno:155173

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCAB45_RAT
AccessioniPrimary (citable) accession number: Q91ZS3
Secondary accession number(s): Q5PQW3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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