ID   S35A4_RAT               Reviewed;         324 AA.
AC   Q91ZR7; Q5RKL6;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   24-JAN-2024, entry version 110.
DE   RecName: Full=Probable UDP-sugar transporter protein SLC35A4 {ECO:0000305};
DE   AltName: Full=Complex leucine repeat protein {ECO:0000303|PubMed:12124754};
DE   AltName: Full=Solute carrier family 35 member A4 {ECO:0000312|RGD:628792};
GN   Name=Slc35a4 {ECO:0000312|RGD:628792};
GN   Synonyms=Clrp {ECO:0000303|PubMed:12124754};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RX   PubMed=12124754; DOI=10.1002/neu.10076;
RA   Perez-Marquez J., Reguillo B., Paniagua R.;
RT   "Cloning of the cDNA and mRNA expression of CLRP, a complex leucine repeat
RT   protein of the Golgi apparatus expressed by specific neurons of the rat
RT   brain.";
RL   J. Neurobiol. 52:166-173(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Heart;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Mediates the transport of CDP-ribitol (By similarity). Does
CC       not exhibit CMP-sialic acid, UDP-galactose and UDP-N-acetylglucosamine
CC       transport activity (By similarity). {ECO:0000250|UniProtKB:Q96G79}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=CDP(out) + CDP-L-ribitol(in) = CDP(in) + CDP-L-ribitol(out);
CC         Xref=Rhea:RHEA:71579, ChEBI:CHEBI:57608, ChEBI:CHEBI:58069;
CC         Evidence={ECO:0000250|UniProtKB:Q96G79};
CC   -!- SUBUNIT: Found in a complex with SLC35A2 and SLC35A3.
CC       {ECO:0000250|UniProtKB:Q96G79}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000269|PubMed:12124754}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Expressed in the kidney, lung, testis, and
CC       prostate. Expressed in the brain by sets of neurons, such as the
CC       pyramidal cells of the cortex, the Purkinje cells of the cerebellum,
CC       and the motoneurons of the brainstem. {ECO:0000269|PubMed:12124754}.
CC   -!- DEVELOPMENTAL STAGE: Expressed at 15 dpc. Expression is maintained
CC       until postnatal day 10 and decreases in adults.
CC       {ECO:0000269|PubMed:12124754}.
CC   -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35A
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AF406814; AAK96221.1; -; mRNA.
DR   EMBL; BC085695; AAH85695.1; -; mRNA.
DR   EMBL; BC086986; AAH86986.1; -; mRNA.
DR   RefSeq; NP_671481.2; NM_147140.2.
DR   RefSeq; XP_006254590.1; XM_006254528.2.
DR   RefSeq; XP_006254591.1; XM_006254529.3.
DR   RefSeq; XP_008770241.1; XM_008772019.2.
DR   AlphaFoldDB; Q91ZR7; -.
DR   SMR; Q91ZR7; -.
DR   STRING; 10116.ENSRNOP00000003878; -.
DR   PhosphoSitePlus; Q91ZR7; -.
DR   PaxDb; 10116-ENSRNOP00000003878; -.
DR   Ensembl; ENSRNOT00000003878.5; ENSRNOP00000003878.4; ENSRNOG00000002908.6.
DR   Ensembl; ENSRNOT00000100525.1; ENSRNOP00000096880.1; ENSRNOG00000002908.6.
DR   Ensembl; ENSRNOT00000109501.1; ENSRNOP00000079324.1; ENSRNOG00000002908.6.
DR   Ensembl; ENSRNOT00055039827; ENSRNOP00055032328; ENSRNOG00055023180.
DR   Ensembl; ENSRNOT00055039834; ENSRNOP00055032332; ENSRNOG00055023180.
DR   Ensembl; ENSRNOT00055039847; ENSRNOP00055032335; ENSRNOG00055023180.
DR   Ensembl; ENSRNOT00060035896; ENSRNOP00060029538; ENSRNOG00060020729.
DR   Ensembl; ENSRNOT00060035934; ENSRNOP00060029567; ENSRNOG00060020729.
DR   Ensembl; ENSRNOT00060035981; ENSRNOP00060029608; ENSRNOG00060020729.
DR   Ensembl; ENSRNOT00065021506; ENSRNOP00065016656; ENSRNOG00065013117.
DR   Ensembl; ENSRNOT00065021513; ENSRNOP00065016663; ENSRNOG00065013117.
DR   Ensembl; ENSRNOT00065021523; ENSRNOP00065016672; ENSRNOG00065013117.
DR   GeneID; 257647; -.
DR   KEGG; rno:257647; -.
DR   UCSC; RGD:628792; rat.
DR   AGR; RGD:628792; -.
DR   CTD; 113829; -.
DR   RGD; 628792; Slc35a4.
DR   eggNOG; KOG2234; Eukaryota.
DR   GeneTree; ENSGT00950000182827; -.
DR   HOGENOM; CLU_024645_5_1_1; -.
DR   InParanoid; Q91ZR7; -.
DR   OMA; SSCVVMI; -.
DR   OrthoDB; 4641608at2759; -.
DR   PhylomeDB; Q91ZR7; -.
DR   TreeFam; TF315345; -.
DR   Proteomes; UP000002494; Chromosome 18.
DR   Bgee; ENSRNOG00000002908; Expressed in skeletal muscle tissue and 19 other cell types or tissues.
DR   GO; GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
DR   GO; GO:0000139; C:Golgi membrane; ISS:UniProtKB.
DR   GO; GO:0015165; F:pyrimidine nucleotide-sugar transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR   GO; GO:0032056; P:positive regulation of translation in response to stress; ISO:RGD.
DR   InterPro; IPR007271; Nuc_sug_transpt.
DR   PANTHER; PTHR10231; NUCLEOTIDE-SUGAR TRANSMEMBRANE TRANSPORTER; 1.
DR   PANTHER; PTHR10231:SF96; UDP-SUGAR TRANSPORTER PROTEIN SLC35A4-RELATED; 1.
DR   Pfam; PF04142; Nuc_sug_transp; 1.
DR   PIRSF; PIRSF005799; UDP-gal_transpt; 1.
DR   SUPFAM; SSF103481; Multidrug resistance efflux transporter EmrE; 1.
DR   Genevisible; Q91ZR7; RN.
PE   2: Evidence at transcript level;
KW   Golgi apparatus; Membrane; Reference proteome; Sugar transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..324
FT                   /note="Probable UDP-sugar transporter protein SLC35A4"
FT                   /id="PRO_0000337752"
FT   TOPO_DOM        1..18
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q96G79"
FT   TRANSMEM        19..39
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        40..52
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250|UniProtKB:Q96G79"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        74..85
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q96G79"
FT   TRANSMEM        86..106
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        107..142
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250|UniProtKB:Q96G79"
FT   TRANSMEM        143..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        164..180
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q96G79"
FT   TRANSMEM        181..201
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        202..214
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250|UniProtKB:Q96G79"
FT   TRANSMEM        215..235
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        236..248
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q96G79"
FT   TRANSMEM        249..271
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        272..279
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000250|UniProtKB:Q96G79"
FT   TRANSMEM        280..300
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        301..324
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250|UniProtKB:Q96G79"
FT   CONFLICT        130
FT                   /note="Y -> C (in Ref. 1; AAK96221)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        137
FT                   /note="R -> C (in Ref. 1; AAK96221)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        260
FT                   /note="Q -> H (in Ref. 1; AAK96221)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        275
FT                   /note="S -> I (in Ref. 1; AAK96221)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   324 AA;  34705 MW;  F66D814CD7CB77C6 CRC64;
     MSVEDGGMPG LARPKQARWT LMLFLSTAMY GAHAPFLALC HVDGRVPFRP SSAVLLTELT
     KLLLCAFSLL VGWQTWPQGT PPWRQAAPFA LSALLYGANN NLVIYLQRYM DPSTYQVLSN
     LKIGSTALLY CLCLGHRLSA RQGLALLLLM AAGACYASGG FQEPGNTLPG PRSAAGARPM
     PLHITPLGLL LLILYCLISG LSSVYTELIM KRQRLPLALQ NLFLYTFGVI LNLGLYAGSG
     PGPGFLEGFS GWAVLVVLNQ AVNGLLMSAV MKHGSSITRL FIVSCSLVVN AVLSAVLLQL
     QLTATFFLAA LLIGLAVCLY YGSP
//