Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cation channel sperm-associated protein 1

Gene

Catsper1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Voltage-gated calcium channel that plays a central role in sperm cell hyperactivation. Controls calcium entry to mediate the hyperactivated motility, a step needed for sperm motility which is essential late in the preparation of sperm for fertilization. Activated by intracellular alkalinization.5 Publications

Enzyme regulationi

In contrast to the human ortholog, not activated by progesterone.1 Publication

GO - Molecular functioni

  • calcium activated cation channel activity Source: InterPro
  • voltage-gated calcium channel activity Source: MGI

GO - Biological processi

  • calcium ion transport Source: MGI
  • cell differentiation Source: UniProtKB-KW
  • fusion of sperm to egg plasma membrane Source: MGI
  • multicellular organism development Source: UniProtKB-KW
  • regulation of calcium ion transport Source: MGI
  • regulation of cilium beat frequency involved in ciliary motility Source: MGI
  • spermatogenesis Source: MGI
  • sperm motility Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Calcium channel, Developmental protein, Ion channel, Voltage-gated channel

Keywords - Biological processi

Calcium transport, Differentiation, Ion transport, Spermatogenesis, Transport

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-MMU-1300642. Sperm Motility And Taxes.

Protein family/group databases

TCDBi1.A.1.19.3. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cation channel sperm-associated protein 1
Short name:
CatSper1
Gene namesi
Name:Catsper1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2179947. Catsper1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 349349CytoplasmicSequence analysisAdd
BLAST
Transmembranei350 – 37021Helical; Name=Segment S1Sequence analysisAdd
BLAST
Topological domaini371 – 38313ExtracellularSequence analysisAdd
BLAST
Transmembranei384 – 40421Helical; Name=Segment S2Sequence analysisAdd
BLAST
Topological domaini405 – 41713CytoplasmicSequence analysisAdd
BLAST
Transmembranei418 – 43821Helical; Name=Segment S3Sequence analysisAdd
BLAST
Topological domaini439 – 4479ExtracellularSequence analysis
Transmembranei448 – 46821Helical; Name=Segment S4Sequence analysisAdd
BLAST
Topological domaini469 – 48517CytoplasmicSequence analysisAdd
BLAST
Transmembranei486 – 50621Helical; Name=Segment S5Sequence analysisAdd
BLAST
Topological domaini507 – 52115ExtracellularSequence analysisAdd
BLAST
Intramembranei522 – 54221Helical; Pore-formingSequence analysisAdd
BLAST
Topological domaini543 – 55210ExtracellularSequence analysis
Transmembranei553 – 57321Helical; Name=Segment S6Sequence analysisAdd
BLAST
Topological domaini574 – 686113CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • CatSper complex Source: UniProtKB
  • motile cilium Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cilium, Flagellum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are normal but males are sterile. Male sterility is due to defects in sperm motility unability to fertilize intact eggs. In mice lacking Catsper1, sperm lacks CatSper2 protein, while in mice lacking CatSper1, sperm lacks CatSper1 protein, suggesting that stable expression of CatSper1 protein requires CatSper2 and vice versa.1 Publication

Chemistry

GuidetoPHARMACOLOGYi388.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 686686Cation channel sperm-associated protein 1PRO_0000295675Add
BLAST

Proteomic databases

PaxDbiQ91ZR5.
PRIDEiQ91ZR5.

PTM databases

PhosphoSiteiQ91ZR5.

Expressioni

Tissue specificityi

Testis-specific.1 Publication

Developmental stagei

Detected only after round spermatids are produced at approximatly day 18.2 Publications

Gene expression databases

BgeeiQ91ZR5.
CleanExiMM_CATSPER1.
GenevisibleiQ91ZR5. MM.

Interactioni

Subunit structurei

Heterotetramer; possibly composed of CATSPER1, CATSPER2, CATSPER3 and CATSPER4 (Potential). Interacts with Ca(v)3.3/CACNA1I, leading to suppress T-type calcium channel activity (By similarity). Component of the CatSper complex. Interacts with CATSPER3 and CATSPER4.By similarityCurated4 Publications

Protein-protein interaction databases

BioGridi230434. 6 interactions.
DIPiDIP-60800N.
STRINGi10090.ENSMUSP00000045430.

Structurei

3D structure databases

ProteinModelPortaliQ91ZR5.
SMRiQ91ZR5. Positions 346-578.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi12 – 255244His-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IP4H. Eukaryota.
ENOG410Y55D. LUCA.
GeneTreeiENSGT00830000128247.
HOGENOMiHOG000112051.
InParanoidiQ91ZR5.
KOiK16889.
OMAiNNISHAY.
OrthoDBiEOG7FV3Q9.
PhylomeDBiQ91ZR5.
TreeFamiTF329330.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR028746. CatSper1.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
[Graphical view]
PANTHERiPTHR10037:SF216. PTHR10037:SF216. 3 hits.
PfamiPF00520. Ion_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91ZR5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQSSRRDES YHETHPGSLD PSHQSHPHPH PHPTLHRPNQ GGVYYDSPQH
60 70 80 90 100
GMFQQPYQQH GGFHQQNELQ HLREFSDSHD NAFSHHSYQQ DRAGVSTLPN
110 120 130 140 150
NISHAYGGSH PLAESQHSGG PQSGPRIDPN HHPHQDDPHR PSEPLSHPSS
160 170 180 190 200
TGSHQGTTHQ QYHERSHHLN PQQNRDHADT ISYRSSTRFY RSHAPFSRQE
210 220 230 240 250
RPHLHADHHH EGHHAHSHHG EHPHHKEQRH YHGDHMHHHI HHRSPSASQL
260 270 280 290 300
SHKSHSTLAT SPSHVGSKST ASGARYTFGA RSQIFGKAQS RESLRESASL
310 320 330 340 350
SEGEDHVQKR KKAQRAHKKA HTGNIFQLLW EKISHLLLGL QQMILSLTQS
360 370 380 390 400
LGFETFIFIV VCLNTVILVA QTFTELEIRG EWYFMVLDSI FLSIYVLEAV
410 420 430 440 450
LKLIALGLEY FYDPWNNLDF FIMVMAVLDF VLLQINSLSY SFYNHSLFRI
460 470 480 490 500
LKVFKSMRAL RAIRVLRRLS ILTSLHEVAG TLSGSLPSIT AILTLMFTCL
510 520 530 540 550
FLFSVVLRAL FQDSDPKRFQ NIFTTLFTLF TMLTLDDWSL IYIDNRAQGA
560 570 580 590 600
WYIIPILMIY IVIQYFIFLN LVIAVLVDNF QMALLKGLEK VKLEQAARVH
610 620 630 640 650
EKLLDDSLTD LNKADANAQM TEEALKMQLI EGMFGNMTVK QRVLHFQFLQ
660 670 680
LVAAVEQHQQ KFRSQAYVID ELVDMAFEAG DDDYGK
Length:686
Mass (Da):78,706
Last modified:December 1, 2001 - v1
Checksum:i2E85FFE72B3147BC
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti25 – 251S → SHP in strain: 72.
Natural varianti52 – 521M → R in strain: 2, 47 and 58. 1 Publication
Natural varianti160 – 1601Missing in strain: 47. 1 Publication
Natural varianti270 – 2701T → I in strain: 72. 1 Publication
Natural varianti276 – 2761Y → S in strain: 72. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF407332 mRNA. Translation: AAL14104.1.
DQ021482 Genomic DNA. Translation: AAY63820.1.
DQ021483 Genomic DNA. Translation: AAY63821.1.
DQ021484 Genomic DNA. Translation: AAY63822.1.
DQ021485 Genomic DNA. Translation: AAY63823.1.
DQ021486 Genomic DNA. Translation: AAY63824.1.
DQ021488 Genomic DNA. Translation: AAY63826.1.
DQ021489 Genomic DNA. Translation: AAY63827.1.
DQ021490 Genomic DNA. Translation: AAY63828.1.
DQ021491 Genomic DNA. Translation: AAY63829.1.
DQ021492 Genomic DNA. Translation: AAY63830.1.
CCDSiCCDS29456.1.
RefSeqiNP_647462.1. NM_139301.2.
UniGeneiMm.87321.

Genome annotation databases

EnsembliENSMUST00000043380; ENSMUSP00000045430; ENSMUSG00000038498.
GeneIDi225865.
KEGGimmu:225865.
UCSCiuc008gcp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF407332 mRNA. Translation: AAL14104.1.
DQ021482 Genomic DNA. Translation: AAY63820.1.
DQ021483 Genomic DNA. Translation: AAY63821.1.
DQ021484 Genomic DNA. Translation: AAY63822.1.
DQ021485 Genomic DNA. Translation: AAY63823.1.
DQ021486 Genomic DNA. Translation: AAY63824.1.
DQ021488 Genomic DNA. Translation: AAY63826.1.
DQ021489 Genomic DNA. Translation: AAY63827.1.
DQ021490 Genomic DNA. Translation: AAY63828.1.
DQ021491 Genomic DNA. Translation: AAY63829.1.
DQ021492 Genomic DNA. Translation: AAY63830.1.
CCDSiCCDS29456.1.
RefSeqiNP_647462.1. NM_139301.2.
UniGeneiMm.87321.

3D structure databases

ProteinModelPortaliQ91ZR5.
SMRiQ91ZR5. Positions 346-578.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230434. 6 interactions.
DIPiDIP-60800N.
STRINGi10090.ENSMUSP00000045430.

Chemistry

GuidetoPHARMACOLOGYi388.

Protein family/group databases

TCDBi1.A.1.19.3. the voltage-gated ion channel (vic) superfamily.

PTM databases

PhosphoSiteiQ91ZR5.

Proteomic databases

PaxDbiQ91ZR5.
PRIDEiQ91ZR5.

Protocols and materials databases

DNASUi225865.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043380; ENSMUSP00000045430; ENSMUSG00000038498.
GeneIDi225865.
KEGGimmu:225865.
UCSCiuc008gcp.1. mouse.

Organism-specific databases

CTDi117144.
MGIiMGI:2179947. Catsper1.

Phylogenomic databases

eggNOGiENOG410IP4H. Eukaryota.
ENOG410Y55D. LUCA.
GeneTreeiENSGT00830000128247.
HOGENOMiHOG000112051.
InParanoidiQ91ZR5.
KOiK16889.
OMAiNNISHAY.
OrthoDBiEOG7FV3Q9.
PhylomeDBiQ91ZR5.
TreeFamiTF329330.

Enzyme and pathway databases

ReactomeiR-MMU-1300642. Sperm Motility And Taxes.

Miscellaneous databases

NextBioi377825.
PROiQ91ZR5.
SOURCEiSearch...

Gene expression databases

BgeeiQ91ZR5.
CleanExiMM_CATSPER1.
GenevisibleiQ91ZR5. MM.

Family and domain databases

Gene3Di1.20.120.350. 1 hit.
InterProiIPR028746. CatSper1.
IPR027359. Channel_four-helix_dom.
IPR005821. Ion_trans_dom.
[Graphical view]
PANTHERiPTHR10037:SF216. PTHR10037:SF216. 3 hits.
PfamiPF00520. Ion_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A sperm ion channel required for sperm motility and male fertility."
    Ren D., Navarro B., Perez G., Jackson A.C., Hsu S., Shi Q., Tilly J.L., Clapham D.E.
    Nature 413:603-609(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
  2. "Positive selection for indel substitutions in the rodent sperm protein catsper1."
    Podlaha O., Webb D.M., Tucker P.K., Zhang J.
    Mol. Biol. Evol. 22:1845-1852(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 19-307, VARIANTS HIS-PRO-25 INS; ARG-52; GLN-160 DEL; ILE-270 AND SER-276.
    Strain: 2, 35, 41, 47, 49, 51, 58, 61, 63 and 72.
  3. "CatSper1 required for evoked Ca2+ entry and control of flagellar function in sperm."
    Carlson A.E., Westenbroek R.E., Quill T., Ren D., Clapham D.E., Hille B., Garbers D.L., Babcock D.F.
    Proc. Natl. Acad. Sci. U.S.A. 100:14864-14868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "CatSper gene expression in postnatal development of mouse testis and in subfertile men with deficient sperm motility."
    Nikpoor P., Mowla S.J., Movahedin M., Ziaee S.A.-M., Tiraihi T.
    Hum. Reprod. 19:124-128(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. Cited for: FUNCTION.
  6. "Whole-cell patch-clamp measurements of spermatozoa reveal an alkaline-activated Ca2+ channel."
    Kirichok Y., Navarro B., Clapham D.E.
    Nature 439:737-740(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Contributions of extracellular and intracellular Ca2+ to regulation of sperm motility: release of intracellular stores can hyperactivate CatSper1 and CatSper2 null sperm."
    Marquez B., Ignotz G., Suarez S.S.
    Dev. Biol. 303:214-221(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "CatSperbeta, a novel transmembrane protein in the CatSper channel complex."
    Liu J., Xia J., Cho K.-H., Clapham D.E., Ren D.
    J. Biol. Chem. 282:18945-18952(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH CATSPERB AND HSPA1B.
  9. "Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: relationship to sperm motility."
    Li H.-G., Ding X.-F., Liao A.-H., Kong X.-B., Xiong C.-L.
    Mol. Hum. Reprod. 13:299-306(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  10. "All four CatSper ion channel proteins are required for male fertility and sperm cell hyperactivated motility."
    Qi H., Moran M.M., Navarro B., Chong J.A., Krapivinsky G., Krapivinsky L., Kirichok Y., Ramsey I.S., Quill T.A., Clapham D.E.
    Proc. Natl. Acad. Sci. U.S.A. 104:1219-1223(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CATSPER3 AND CATSPER4.
  11. "A novel, single, transmembrane protein CATSPERG is associated with CATSPER1 channel protein."
    Wang H., Liu J., Cho K.-H., Ren D.
    Biol. Reprod. 81:539-544(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN A COMPLEX WITH CATSPERB; CATSPERG2 AND HSPA1B.
  12. "Progesterone activates the principal Ca2+ channel of human sperm."
    Lishko P.V., Botchkina I.L., Kirichok Y.
    Nature 471:387-391(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF ACTIVATION BY PROGESTERONE AND PGE1.
  13. "A novel gene required for male fertility and functional CATSPER channel formation in spermatozoa."
    Chung J.J., Navarro B., Krapivinsky G., Krapivinsky L., Clapham D.E.
    Nat. Commun. 2:153-153(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CATSPER COMPLEX.
    Strain: C57BL/6J.

Entry informationi

Entry nameiCTSR1_MOUSE
AccessioniPrimary (citable) accession number: Q91ZR5
Secondary accession number(s): Q4PZC3
, Q4PZC4, Q4PZC5, Q4PZC6, Q4PZD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.