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Protein

Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'

Gene

Pde6c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971cGMPBy similarity
Binding sitei116 – 1161cGMPBy similarity
Binding sitei176 – 1761cGMPBy similarity
Active sitei562 – 5621Proton donorBy similarity
Metal bindingi566 – 5661Divalent metal cation 1By similarity
Metal bindingi602 – 6021Divalent metal cation 1By similarity
Metal bindingi603 – 6031Divalent metal cation 1By similarity
Metal bindingi603 – 6031Divalent metal cation 2By similarity
Metal bindingi723 – 7231Divalent metal cation 1By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi169 – 1724cGMPBy similarity

GO - Molecular functioni

  1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
  2. cGMP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. phototransduction, visible light Source: MGI
  2. retinal cone cell development Source: MGI
  3. sensory perception of light stimulus Source: MGI
  4. visual perception Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

cGMP, cGMP-binding, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha' (EC:3.1.4.35)
Alternative name(s):
cGMP phosphodiesterase 6C
Gene namesi
Name:Pde6c
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 19

Organism-specific databases

MGIiMGI:105956. Pde6c.

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 858858Cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha'PRO_0000226068Add
BLAST
Propeptidei859 – 8613Removed in mature formSequence AnalysisPRO_0000370789

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei858 – 8581Cysteine methyl esterSequence Analysis
Lipidationi858 – 8581S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

PRIDEiQ91ZQ1.

PTM databases

PhosphoSiteiQ91ZQ1.

Expressioni

Gene expression databases

BgeeiQ91ZQ1.
GenevestigatoriQ91ZQ1.

Interactioni

Subunit structurei

Composed of two alpha' subunits that are associated with 3 smaller proteins of 11, 13, and 15 kDa.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ91ZQ1.
SMRiQ91ZQ1. Positions 24-818.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini75 – 224150GAF 1Add
BLAST
Domaini256 – 433178GAF 2Add
BLAST

Sequence similaritiesi

Contains 2 GAF domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG290744.
GeneTreeiENSGT00760000119066.
HOGENOMiHOG000007069.
HOVERGENiHBG053539.
InParanoidiQ91ZQ1.
KOiK13757.
OMAiIVMGKEV.
OrthoDBiEOG7BGHK1.
PhylomeDBiQ91ZQ1.
TreeFamiTF316499.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q91ZQ1-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGEISQEAVE RYLEKNPCFA KEYFDKKLRV EALGVIFKNS HAGVQTGLSL
60 70 80 90 100
PEMTQVEESA VCLELLQCMQ DEAGSAEQMA HRALQRLAQL LQADCCSMFS
110 120 130 140 150
CRARNGIPEV ASRLLNVTPT SKFEDNLVAP DREVVFPLDI GIVGWVAHVK
160 170 180 190 200
KALNVSDVKK NSHFSDFMDK QTGYVTRNLL AVPIVAGKEV LAVVMAVNKI
210 220 230 240 250
SAPEFSKQDE EVFSKYLSFV AVALRLQHTS YLYSVESRRS QILMWSANKV
260 270 280 290 300
FEELTDVERQ FHKALYTIRT YLNCDRYSIG LLDMTKEKEF YDEWPIKLGE
310 320 330 340 350
VEPYKGPKTP DGREIIFYKI IDYILHGKEE INVIPSPPAD HWTLVSGLPT
360 370 380 390 400
YVAENGFICN MLNAPADEYF TFQKGPVDET GWVIKNVLSL PIVNKKEDIV
410 420 430 440 450
GVATFYNRKD GKPFDEHDEH ITETLTQFLG WSLLNTDTYE RVNKLESRKD
460 470 480 490 500
IAQEMVMNLT KATPDEISSI LKFKEKLNVE VIEECEERQL LAILKEDLPD
510 520 530 540 550
PRTADLYEFC FSDFPITEHE LVKCGLRLFL EINVVEKFKV PVEVLTRWMY
560 570 580 590 600
TVRKGYRPVT YHNWRHGFNV GQTMFTLLMT GRLKKYYTDL EAFAMLAAAF
610 620 630 640 650
CHDIDHRGTN NLYQMKSTSP LARLHGTSIL ERHHLEYSKT LLQDESLNIF
660 670 680 690 700
QNLNKRQFET VIHLFEVAII ATDLALYFKK RTMFQKIVDT CEQMQSEEET
710 720 730 740 750
IKYVTSDPTK KEVIMAMMMT ACDLSAITKP WEVQSQVALL VANEFWEQGD
760 770 780 790 800
LERTVLQQQP IPMMDRSKKD ELPKLQVGFI DFVCTFVYKE FSRFHGEITP
810 820 830 840 850
MLNGLQNNRV EWKSLAEEYE AKVKVTEEEA GKQEEEASDG KAATDLGGSA
860
EDKKSKTCLM L
Length:861
Mass (Da):98,785
Last modified:December 1, 2001 - v1
Checksum:iC582D78114652B5B
GO
Isoform 2 (identifier: Q91ZQ1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     737-761: Missing.

Note: No experimental confirmation available.

Show »
Length:836
Mass (Da):95,906
Checksum:iA8D60FAAFE6C9D9E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei737 – 76125Missing in isoform 2. 1 PublicationVSP_017422Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411063 mRNA. Translation: AAK96254.1.
BC027050 mRNA. Translation: AAH27050.1.
CCDSiCCDS37971.1. [Q91ZQ1-1]
CCDS50431.1. [Q91ZQ1-2]
RefSeqiNP_001164430.1. NM_001170959.1. [Q91ZQ1-2]
NP_291092.1. NM_033614.2. [Q91ZQ1-1]
UniGeneiMm.450141.

Genome annotation databases

EnsembliENSMUST00000025956; ENSMUSP00000025956; ENSMUSG00000024992. [Q91ZQ1-1]
ENSMUST00000112329; ENSMUSP00000107948; ENSMUSG00000024992. [Q91ZQ1-2]
GeneIDi110855.
KEGGimmu:110855.
UCSCiuc008hjf.2. mouse. [Q91ZQ1-1]
uc012blg.1. mouse. [Q91ZQ1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF411063 mRNA. Translation: AAK96254.1.
BC027050 mRNA. Translation: AAH27050.1.
CCDSiCCDS37971.1. [Q91ZQ1-1]
CCDS50431.1. [Q91ZQ1-2]
RefSeqiNP_001164430.1. NM_001170959.1. [Q91ZQ1-2]
NP_291092.1. NM_033614.2. [Q91ZQ1-1]
UniGeneiMm.450141.

3D structure databases

ProteinModelPortaliQ91ZQ1.
SMRiQ91ZQ1. Positions 24-818.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ91ZQ1.

Proteomic databases

PRIDEiQ91ZQ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025956; ENSMUSP00000025956; ENSMUSG00000024992. [Q91ZQ1-1]
ENSMUST00000112329; ENSMUSP00000107948; ENSMUSG00000024992. [Q91ZQ1-2]
GeneIDi110855.
KEGGimmu:110855.
UCSCiuc008hjf.2. mouse. [Q91ZQ1-1]
uc012blg.1. mouse. [Q91ZQ1-2]

Organism-specific databases

CTDi5146.
MGIiMGI:105956. Pde6c.

Phylogenomic databases

eggNOGiNOG290744.
GeneTreeiENSGT00760000119066.
HOGENOMiHOG000007069.
HOVERGENiHBG053539.
InParanoidiQ91ZQ1.
KOiK13757.
OMAiIVMGKEV.
OrthoDBiEOG7BGHK1.
PhylomeDBiQ91ZQ1.
TreeFamiTF316499.

Miscellaneous databases

ChiTaRSiPde6c. mouse.
NextBioi364793.
PROiQ91ZQ1.
SOURCEiSearch...

Gene expression databases

BgeeiQ91ZQ1.
GenevestigatoriQ91ZQ1.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A sequence alteration in Pde6c gene causes cone photoreceptor function loss (cpfl1) in mice."
    Chang B., Hawes N.L., Hurd R.E., Davisson M.T., Nusinowitz S., Heckenlively J.R.
    Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Retina.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Eye.

Entry informationi

Entry nameiPDE6C_MOUSE
AccessioniPrimary (citable) accession number: Q91ZQ1
Secondary accession number(s): Q8R0D4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: December 1, 2001
Last modified: January 7, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.