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Protein

Diencephalon/mesencephalon homeobox protein 1

Gene

Dmbx1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a transcriptional repressor. May repress OTX2-mediated transactivation by forming a heterodimer with OTX2 on the P3C (5'-TAATCCGATTA-3') sequence. Required for brain development, neonatal survival, postnatal growth, and nursing ability.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi71 – 13060HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: MGI
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: UniProtKB
  • transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding Source: NTNU_SB
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

  • adult feeding behavior Source: MGI
  • adult locomotory behavior Source: MGI
  • brain development Source: UniProtKB
  • central nervous system development Source: UniProtKB
  • developmental growth Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Diencephalon/mesencephalon homeobox protein 1
Alternative name(s):
Diencephalon/mesencephalon-expressed brain homeobox gene 1 protein
Orthodenticle homolog 3
Paired-like homeobox protein DMBX1
Paired-type homeobox Atx
Gene namesi
Name:Dmbx1Imported
Synonyms:CdmxImported, Mbx1 Publication, Otx3Imported, PaxBImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2153518. Dmbx1.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotationBy similarity

GO - Cellular componenti

  • nucleus Source: UniProtKB
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 381381Diencephalon/mesencephalon homeobox protein 1PRO_0000262955Add
BLAST

Proteomic databases

PaxDbiQ91ZK4.
PRIDEiQ91ZK4.

PTM databases

iPTMnetiQ91ZK4.
PhosphoSiteiQ91ZK4.

Expressioni

Tissue specificityi

Expressed in adult brain, stomach, and testis. Expressed in the developing diencephalon, midbrain and hindbrain. During limb development, expressed in a temporal pattern with expression being first restricted to the forelimbs and then subsequently to the hindlimbs.4 Publications

Developmental stagei

Detected in embryo from E10.5 to 13.5 dpc. Strongly expressed from 9.5 to 11.5 dpc in the diencephalon, mesencephalon, metencephalon, myeloencephalon, and the developing eye.3 Publications

Gene expression databases

BgeeiQ91ZK4.
CleanExiMM_DMBX1.
GenevisibleiQ91ZK4. MM.

Interactioni

Subunit structurei

Homodimer or heterodimer. Forms heterodimers with OTX2.1 Publication

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi228255. 1 interaction.
MINTiMINT-1347271.
STRINGi10090.ENSMUSP00000120320.

Structurei

3D structure databases

ProteinModelPortaliQ91ZK4.
SMRiQ91ZK4. Positions 78-127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 156156Interacts with OTX2 and is required for repressor activity1 PublicationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi358 – 37114OARPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the paired homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00820000126963.
HOGENOMiHOG000261650.
HOVERGENiHBG061416.
InParanoidiQ91ZK4.
OMAiCQSYYQT.
OrthoDBiEOG7D2FF8.
PhylomeDBiQ91ZK4.
TreeFamiTF351609.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 14 Publications (identifier: Q91ZK4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQHYGVNGYS LHAMNSLSAM YNLHQQAAQQ AQHAPDYRPS VHALTLAERL
60 70 80 90 100
AGCTFQDIIL EARYGSQHRK QRRSRTAFTA QQLEALEKTF QKTHYPDVVM
110 120 130 140 150
RERLAMCTNL PEARVQVWFK NRRAKFRKKQ RSLQKEQLQK QKEAEGSHGE
160 170 180 190 200
GKVEAPASDT QLETEQPPGL PSGDPPAELQ LSLSEQSASE SAPEDQLDRE
210 220 230 240 250
EDSRAEEPKA EKSPGSESKV PGCKRGSPKA DSPGSLAITP AAPGGGLLGP
260 270 280 290 300
SHSYSSSPLS LFRLQEQFRQ HMAATNNLMH YSSFEVGGPA PAAAAAAAAA
310 320 330 340 350
VPYLGVNMAP LSSLHCQSYY QSLSAAAAAH QGVWGSPLLP APPTGLAPAS
360 370 380
AALNSKTTSI ENLRLRAKQH AASLGLDTLP N
Length:381
Mass (Da):41,115
Last modified:December 1, 2001 - v1
Checksum:iF180D3BAE1D2CFAE
GO
Isoform 23 Publications (identifier: Q91ZK4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     52-56: Missing.

Show »
Length:376
Mass (Da):40,578
Checksum:i48959A8120BB1B70
GO

Sequence cautioni

The sequence BAC00919.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti206 – 2061E → G in AAN76724 (PubMed:11914943).Curated
Sequence conflicti279 – 2791M → V in AAL66343 (PubMed:12175514).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei52 – 565Missing in isoform 2. 4 PublicationsVSP_052252

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448118 mRNA. Translation: AAN76724.1.
AF499446 mRNA. Translation: AAM78514.1.
AB037698 mRNA. Translation: BAC00919.1. Different initiation.
AF421857 mRNA. Translation: AAL30508.1.
AF421858 mRNA. Translation: AAL30509.1.
AF463513 mRNA. Translation: AAL68836.1.
AL627105 Genomic DNA. Translation: CAM22665.1.
BC050912 mRNA. Translation: AAH50912.1.
AF461039 mRNA. Translation: AAL66343.1.
CCDSiCCDS18500.1. [Q91ZK4-2]
RefSeqiNP_001020738.1. NM_001025567.1. [Q91ZK4-2]
NP_570935.3. NM_130865.3. [Q91ZK4-1]
UniGeneiMm.213206.
Mm.413175.

Genome annotation databases

EnsembliENSMUST00000064806; ENSMUSP00000134457; ENSMUSG00000028707. [Q91ZK4-2]
ENSMUST00000084338; ENSMUSP00000081366; ENSMUSG00000028707. [Q91ZK4-1]
ENSMUST00000124071; ENSMUSP00000120320; ENSMUSG00000028707. [Q91ZK4-2]
GeneIDi140477.
KEGGimmu:140477.
UCSCiuc008ufp.1. mouse. [Q91ZK4-2]
uc008ufr.1. mouse. [Q91ZK4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF448118 mRNA. Translation: AAN76724.1.
AF499446 mRNA. Translation: AAM78514.1.
AB037698 mRNA. Translation: BAC00919.1. Different initiation.
AF421857 mRNA. Translation: AAL30508.1.
AF421858 mRNA. Translation: AAL30509.1.
AF463513 mRNA. Translation: AAL68836.1.
AL627105 Genomic DNA. Translation: CAM22665.1.
BC050912 mRNA. Translation: AAH50912.1.
AF461039 mRNA. Translation: AAL66343.1.
CCDSiCCDS18500.1. [Q91ZK4-2]
RefSeqiNP_001020738.1. NM_001025567.1. [Q91ZK4-2]
NP_570935.3. NM_130865.3. [Q91ZK4-1]
UniGeneiMm.213206.
Mm.413175.

3D structure databases

ProteinModelPortaliQ91ZK4.
SMRiQ91ZK4. Positions 78-127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228255. 1 interaction.
MINTiMINT-1347271.
STRINGi10090.ENSMUSP00000120320.

PTM databases

iPTMnetiQ91ZK4.
PhosphoSiteiQ91ZK4.

Proteomic databases

PaxDbiQ91ZK4.
PRIDEiQ91ZK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064806; ENSMUSP00000134457; ENSMUSG00000028707. [Q91ZK4-2]
ENSMUST00000084338; ENSMUSP00000081366; ENSMUSG00000028707. [Q91ZK4-1]
ENSMUST00000124071; ENSMUSP00000120320; ENSMUSG00000028707. [Q91ZK4-2]
GeneIDi140477.
KEGGimmu:140477.
UCSCiuc008ufp.1. mouse. [Q91ZK4-2]
uc008ufr.1. mouse. [Q91ZK4-1]

Organism-specific databases

CTDi127343.
MGIiMGI:2153518. Dmbx1.

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00820000126963.
HOGENOMiHOG000261650.
HOVERGENiHBG061416.
InParanoidiQ91ZK4.
OMAiCQSYYQT.
OrthoDBiEOG7D2FF8.
PhylomeDBiQ91ZK4.
TreeFamiTF351609.

Miscellaneous databases

NextBioi369772.
PROiQ91ZK4.
SOURCEiSearch...

Gene expression databases

BgeeiQ91ZK4.
CleanExiMM_DMBX1.
GenevisibleiQ91ZK4. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR003654. OAR_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03826. OAR. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS50803. OAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Embryo1 Publication.
  2. "An orphan PRD class homeobox gene expressed in mouse brain and limb development."
    Takahashi T., Holland P.W.H., Cohn M.J., Shimizu K., Kurokawa M., Hirai H.
    Dev. Genes Evol. 212:293-297(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
    Strain: C57BL/6JImported.
  3. "Identification, tissue expression, and functional characterization of Otx3, a novel member of the Otx family."
    Zhang Y., Miki T., Iwanaga T., Koseki Y., Okuno M., Sunaga Y., Ozaki N., Yano H., Koseki H., Seino S.
    J. Biol. Chem. 277:28065-28069(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, DEVELOPMENTAL STAGE.
  4. "Dmbx1, a novel evolutionarily conserved paired-like homeobox gene expressed in the brain of mouse embryos."
    Ohtoshi A., Nishijima I., Justice M.J., Behringer R.R.
    Mech. Dev. 110:241-244(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: SWR/JImported.
  5. "Dmbx1 is a paired-box containing gene specifically expressed in the caudal most brain structures."
    Broccoli V., Colombo E., Cossu G.
    Mech. Dev. 114:219-223(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE.
    Strain: CD-1Imported.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6JImported.
    Tissue: BrainImported.
  8. "The paired-type homeobox gene Dmbx1 marks the midbrain and pretectum."
    Gogoi R.N., Schubert F.R., Martinez-Barbera J.-P., Acampora D., Simeone A., Lumsden A.
    Mech. Dev. 114:213-217(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-282 (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Embryo1 Publication.
  9. "Neonatal lethality, dwarfism, and abnormal brain development in Dmbx1 mutant mice."
    Ohtoshi A., Behringer R.R.
    Mol. Cell. Biol. 24:7548-7558(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. Cited for: FUNCTION, INTERACTION WITH OTX2, DOMAIN.

Entry informationi

Entry nameiDMBX1_MOUSE
AccessioniPrimary (citable) accession number: Q91ZK4
Secondary accession number(s): B1ASG1
, Q8CH43, Q8K4U6, Q8VHG8, Q91ZK3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.