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Protein

Hyaluronidase-1

Gene

Hyal1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.1 Publication

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

pH dependencei

Optimum pH is 3.5-4.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei159Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiR-MMU-2024101. CS/DS degradation.
R-MMU-2160916. Hyaluronan uptake and degradation.

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase-1 (EC:3.2.1.35)
Short name:
Hyal-1
Alternative name(s):
Hyaluronoglucosaminidase-1
Gene namesi
Name:Hyal1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:96298. Hyal1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 52Sequence analysisAdd BLAST52
ChainiPRO_000004262453 – 462Hyaluronidase-1Add BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi71 ↔ 361By similarity
Glycosylationi98N-linked (GlcNAc...)Sequence analysis1
Glycosylationi127N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi235 ↔ 249By similarity
Glycosylationi244N-linked (GlcNAc...)Sequence analysis1
Glycosylationi265N-linked (GlcNAc...)Sequence analysis1
Glycosylationi378N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi386 ↔ 397By similarity
Disulfide bondi391 ↔ 446By similarity
Disulfide bondi448 ↔ 457By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ91ZJ9.
PRIDEiQ91ZJ9.

PTM databases

PhosphoSitePlusiQ91ZJ9.

Expressioni

Tissue specificityi

Highly expressed in liver, kidney, lung and skin.2 Publications

Developmental stagei

Detected in embryos of all developmental stages, with high level at the 7 day stage.1 Publication

Gene expression databases

BgeeiENSMUSG00000010051.
CleanExiMM_HYAL1.
ExpressionAtlasiQ91ZJ9. baseline and differential.
GenevisibleiQ91ZJ9. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010195.

Structurei

3D structure databases

ProteinModelPortaliQ91ZJ9.
SMRiQ91ZJ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini446 – 457EGF-likeAdd BLAST12

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated
Contains 1 EGF-like domain.Curated

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

eggNOGiENOG410IECJ. Eukaryota.
ENOG410XPZT. LUCA.
GeneTreeiENSGT00550000074476.
HOVERGENiHBG052053.
InParanoidiQ91ZJ9.
KOiK01197.
OMAiPNYTGQC.
OrthoDBiEOG091G064G.
TreeFamiTF321598.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91ZJ9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLGLTQHAQK VWRMKPFSPE VSPGSSPATA GHLLRISTLF LTLLELAQVC
60 70 80 90 100
RGSVVSNRPF ITVWNGDTHW CLTEYGVDVD VSVFDVVANK EQSFQGSNMT
110 120 130 140 150
IFYREELGTY PYYTPTGEPV FGGLPQNASL VTHLAHTFQD IKAAMPEPDF
160 170 180 190 200
SGLAVIDWEA WRPRWAFNWD SKDIYRQRSM ELVQAEHPDW PETLVEAAAK
210 220 230 240 250
NQFQEAAEAW MAGTLQLGQV LRPRGLWGYY GFPDCYNNDF LSLNYTGQCP
260 270 280 290 300
VFVRDQNDQL GWLWNQSYAL YPSIYLPAAL MGTEKSQMYV RHRVQEALRV
310 320 330 340 350
AIVSRDPHVP VMPYVQIFYE MTDYLLPLEE LEHSLGESAA QGVAGAVLWL
360 370 380 390 400
SSDKTSTKES CQAIKAYMDS TLGPFIVNVT SAALLCSEAL CSGHGRCVRH
410 420 430 440 450
PSYPEALLTL NPASFSIELT HDGRPPSLKG TLSLKDRAQM AMKFRCRCYR
460
GWRGKWCDKR GM
Length:462
Mass (Da):52,109
Last modified:July 27, 2011 - v3
Checksum:iDCE7339D7E0BCB9D
GO
Isoform 2 (identifier: Q91ZJ9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     455-462: KWCDKRGM → GSST

Note: No experimental confirmation available.
Show »
Length:458
Mass (Da):51,436
Checksum:iA510CD568AF4FA5F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16P → S in AAC15949 (PubMed:9503017).Curated1
Sequence conflicti16P → S in AAL17822 (PubMed:11960552).Curated1
Sequence conflicti247G → R in AAC15949 (PubMed:9503017).Curated1
Sequence conflicti284E → G in AAL17822 (PubMed:11960552).Curated1
Sequence conflicti284E → G in AAL54881 (Ref. 3) Curated1
Sequence conflicti284E → G in AAL57173 (Ref. 3) Curated1
Sequence conflicti450R → C in AAL17822 (PubMed:11960552).Curated1
Sequence conflicti450R → C in AAL54881 (Ref. 3) Curated1
Sequence conflicti450R → C in AAL57173 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_015923455 – 462KWCDKRGM → GSST in isoform 2. 1 Publication8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011567 mRNA. Translation: AAC15949.1.
AF422176 mRNA. Translation: AAL17822.1.
AF069741 Genomic DNA. Translation: AAL54881.1.
AF338323 Genomic DNA. Translation: AAL57173.1.
AK028942 mRNA. Translation: BAC26206.1.
AL672219 Genomic DNA. Translation: CAP19319.1.
BC021636 mRNA. Translation: AAH21636.1.
AF417496 mRNA. Translation: AAM14428.1.
AF417497 mRNA. Translation: AAM14430.1.
AF417498 mRNA. Translation: AAM14432.1.
CCDSiCCDS23497.1. [Q91ZJ9-1]
RefSeqiNP_032343.2. NM_008317.5. [Q91ZJ9-1]
UniGeneiMm.475658.

Genome annotation databases

EnsembliENSMUST00000010195; ENSMUSP00000010195; ENSMUSG00000010051. [Q91ZJ9-1]
ENSMUST00000112387; ENSMUSP00000108006; ENSMUSG00000010051. [Q91ZJ9-2]
GeneIDi15586.
KEGGimmu:15586.
UCSCiuc009rlv.1. mouse. [Q91ZJ9-1]
uc009rlw.1. mouse. [Q91ZJ9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF011567 mRNA. Translation: AAC15949.1.
AF422176 mRNA. Translation: AAL17822.1.
AF069741 Genomic DNA. Translation: AAL54881.1.
AF338323 Genomic DNA. Translation: AAL57173.1.
AK028942 mRNA. Translation: BAC26206.1.
AL672219 Genomic DNA. Translation: CAP19319.1.
BC021636 mRNA. Translation: AAH21636.1.
AF417496 mRNA. Translation: AAM14428.1.
AF417497 mRNA. Translation: AAM14430.1.
AF417498 mRNA. Translation: AAM14432.1.
CCDSiCCDS23497.1. [Q91ZJ9-1]
RefSeqiNP_032343.2. NM_008317.5. [Q91ZJ9-1]
UniGeneiMm.475658.

3D structure databases

ProteinModelPortaliQ91ZJ9.
SMRiQ91ZJ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010195.

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

PTM databases

PhosphoSitePlusiQ91ZJ9.

Proteomic databases

PaxDbiQ91ZJ9.
PRIDEiQ91ZJ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010195; ENSMUSP00000010195; ENSMUSG00000010051. [Q91ZJ9-1]
ENSMUST00000112387; ENSMUSP00000108006; ENSMUSG00000010051. [Q91ZJ9-2]
GeneIDi15586.
KEGGimmu:15586.
UCSCiuc009rlv.1. mouse. [Q91ZJ9-1]
uc009rlw.1. mouse. [Q91ZJ9-2]

Organism-specific databases

CTDi3373.
MGIiMGI:96298. Hyal1.

Phylogenomic databases

eggNOGiENOG410IECJ. Eukaryota.
ENOG410XPZT. LUCA.
GeneTreeiENSGT00550000074476.
HOVERGENiHBG052053.
InParanoidiQ91ZJ9.
KOiK01197.
OMAiPNYTGQC.
OrthoDBiEOG091G064G.
TreeFamiTF321598.

Enzyme and pathway databases

ReactomeiR-MMU-2024101. CS/DS degradation.
R-MMU-2160916. Hyaluronan uptake and degradation.

Miscellaneous databases

PROiQ91ZJ9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000010051.
CleanExiMM_HYAL1.
ExpressionAtlasiQ91ZJ9. baseline and differential.
GenevisibleiQ91ZJ9. MM.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHYAL1_MOUSE
AccessioniPrimary (citable) accession number: Q91ZJ9
Secondary accession number(s): B1AV90
, O70229, Q8CE62, Q8QZX3, Q8VBW7, Q8VDK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.