Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Crossover junction endonuclease MUS81

Gene

Mus81

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.3 Publications

Cofactori

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-5693568. Resolution of D-loop Structures through Holliday Junction Intermediates.
R-MMU-6783310. Fanconi Anemia Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endonuclease MUS81 (EC:3.1.22.-)
Gene namesi
Name:Mus81
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1918961. Mus81.

Subcellular locationi

  • Nucleusnucleolus

  • Note: Recruited to foci of DNA damage in S-phase cells.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 551551Crossover junction endonuclease MUS81PRO_0000198859Add
BLAST

Proteomic databases

EPDiQ91ZJ0.
PaxDbiQ91ZJ0.
PRIDEiQ91ZJ0.

PTM databases

PhosphoSiteiQ91ZJ0.

Expressioni

Gene expression databases

BgeeiQ91ZJ0.
CleanExiMM_MUS81.
ExpressionAtlasiQ91ZJ0. baseline and differential.
GenevisibleiQ91ZJ0. MM.

Interactioni

Subunit structurei

May self-associate. Interacts with CHEK2. Interacts with BLM, and this interaction may stimulate the endonuclease activity of MUS81. Interacts with EME2. Interacts with SLX4/BTBD12; this interaction is direct and links the MUS81-EME1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair. Interacts with DCLRE1B/Apollo (By similarity). Interacts with EME1.By similarity1 Publication

Protein-protein interaction databases

BioGridi214873. 17 interactions.
IntActiQ91ZJ0. 15 interactions.
STRINGi10090.ENSMUSP00000114895.

Structurei

Secondary structure

1
551
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi20 – 3516Combined sources
Helixi40 – 5213Combined sources
Helixi60 – 645Combined sources
Turni67 – 693Combined sources
Helixi71 – 8717Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KP7NMR-A11-90[»]
ProteinModelPortaliQ91ZJ0.
SMRiQ91ZJ0. Positions 11-90, 130-228, 256-551.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91ZJ0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini270 – 372103ERCC4Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 244120Interaction with BLMBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the XPF family.Curated
Contains 1 ERCC4 domain.Curated

Phylogenomic databases

eggNOGiKOG2379. Eukaryota.
COG1948. LUCA.
GeneTreeiENSGT00390000005498.
HOGENOMiHOG000113699.
HOVERGENiHBG052538.
InParanoidiQ91ZJ0.
KOiK08991.
OMAiVLRTHQP.
OrthoDBiEOG7TXKGV.
PhylomeDBiQ91ZJ0.
TreeFamiTF315113.

Family and domain databases

Gene3Di1.10.8.310. 1 hit.
3.40.50.10130. 1 hit.
InterProiIPR010996. DNA_pol_b-like_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR033309. Mus81.
IPR027420. PolB_N.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PANTHERiPTHR13451:SF0. PTHR13451:SF0. 1 hit.
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

Q91ZJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEPVRLGRK RPLPVCPNPL FVRWLTEWRD EAASRGRHTR FVFQKALRSL
60 70 80 90 100
QRYPLPLRSG KEAKILQHFG DRLCRMLDEK LKQHLASGGD HAPSSPSGKK
110 120 130 140 150
GASKGPPEQV QDSSMPVPTQ PQAGSTSVGY WPAQNSGARE ILLQLYREHL
160 170 180 190 200
NSDGHSFLTK EELLQKCAQK TPRVVPGSSK PWPALRSLLH RNLILGTHRP
210 220 230 240 250
ARYALTPEGL ELAQKLAEAE GLSTRHAGFR PEEHHGEDSA VPEALSEPGT
260 270 280 290 300
TEGAVQQRPL ELRPSEYRVL LCVDIGETRG AGHRPEMLRE LQRLRVPHTV
310 320 330 340 350
RKLHVGDFVW VAQETRPRDP ERPGELVLDH IVERKRLDDL CSSIIDGRFR
360 370 380 390 400
EQKFRLKRCG LGHRVYLVEE HGSVHNLSLP ESTLLQAVTN TQVIDGFFVK
410 420 430 440 450
RTMDIKESAG YLALLTKGLE RLYQGHTLRS RPWGAPGAAE SEAKPSTNPL
460 470 480 490 500
CSLLTFSDFN AEAVKNKAQS VREVFARQLM QVRGLSGEKA AAVVDRYSTP
510 520 530 540 550
ASLLAAYDAC ATAKEQEMLL STIKCGRLQR NLGPALSRTL YQLYCSHSPL

S
Length:551
Mass (Da):61,531
Last modified:February 5, 2008 - v2
Checksum:i98A5EE72E656C684
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41P → R in AAH26560 (PubMed:15489334).Curated
Sequence conflicti227 – 2271A → D in AAH26560 (PubMed:15489334).Curated
Sequence conflicti285 – 2851P → L in AAL28066 (PubMed:11741546).Curated
Sequence conflicti409 – 4091A → V in AAL28066 (PubMed:11741546).Curated
Sequence conflicti513 – 5131A → T in AAH26560 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF425647 mRNA. Translation: AAL28066.1.
BC026560 mRNA. Translation: AAH26560.1.
CCDSiCCDS29467.1.
RefSeqiNP_082153.3. NM_027877.3.
UniGeneiMm.27697.

Genome annotation databases

EnsembliENSMUST00000124334; ENSMUSP00000114895; ENSMUSG00000024906.
GeneIDi71711.
KEGGimmu:71711.
UCSCiuc008gdn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF425647 mRNA. Translation: AAL28066.1.
BC026560 mRNA. Translation: AAH26560.1.
CCDSiCCDS29467.1.
RefSeqiNP_082153.3. NM_027877.3.
UniGeneiMm.27697.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KP7NMR-A11-90[»]
ProteinModelPortaliQ91ZJ0.
SMRiQ91ZJ0. Positions 11-90, 130-228, 256-551.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214873. 17 interactions.
IntActiQ91ZJ0. 15 interactions.
STRINGi10090.ENSMUSP00000114895.

PTM databases

PhosphoSiteiQ91ZJ0.

Proteomic databases

EPDiQ91ZJ0.
PaxDbiQ91ZJ0.
PRIDEiQ91ZJ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000124334; ENSMUSP00000114895; ENSMUSG00000024906.
GeneIDi71711.
KEGGimmu:71711.
UCSCiuc008gdn.2. mouse.

Organism-specific databases

CTDi80198.
MGIiMGI:1918961. Mus81.

Phylogenomic databases

eggNOGiKOG2379. Eukaryota.
COG1948. LUCA.
GeneTreeiENSGT00390000005498.
HOGENOMiHOG000113699.
HOVERGENiHBG052538.
InParanoidiQ91ZJ0.
KOiK08991.
OMAiVLRTHQP.
OrthoDBiEOG7TXKGV.
PhylomeDBiQ91ZJ0.
TreeFamiTF315113.

Enzyme and pathway databases

ReactomeiR-MMU-5693568. Resolution of D-loop Structures through Holliday Junction Intermediates.
R-MMU-6783310. Fanconi Anemia Pathway.

Miscellaneous databases

EvolutionaryTraceiQ91ZJ0.
NextBioi334299.
PROiQ91ZJ0.
SOURCEiSearch...

Gene expression databases

BgeeiQ91ZJ0.
CleanExiMM_MUS81.
ExpressionAtlasiQ91ZJ0. baseline and differential.
GenevisibleiQ91ZJ0. MM.

Family and domain databases

Gene3Di1.10.8.310. 1 hit.
3.40.50.10130. 1 hit.
InterProiIPR010996. DNA_pol_b-like_N.
IPR020819. DNA_repair_nuc_XPF/helicase.
IPR006166. ERCC4_domain.
IPR033309. Mus81.
IPR027420. PolB_N.
IPR011335. Restrct_endonuc-II-like.
[Graphical view]
PANTHERiPTHR13451:SF0. PTHR13451:SF0. 1 hit.
PfamiPF02732. ERCC4. 1 hit.
[Graphical view]
SMARTiSM00891. ERCC4. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52980. SSF52980. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  3. Cited for: FUNCTION, INTERACTION WITH EME1.
  4. Cited for: FUNCTION.
  5. "Disruption of murine Mus81 increases genomic instability and DNA damage sensitivity but does not promote tumorigenesis."
    Dendouga N., Gao H., Moechars D., Janicot M., Vialard J., McGowan C.H.
    Mol. Cell. Biol. 25:7569-7579(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiMUS81_MOUSE
AccessioniPrimary (citable) accession number: Q91ZJ0
Secondary accession number(s): Q8R356
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: February 5, 2008
Last modified: May 11, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.