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Protein

Thyroxine 5-deiodinase

Gene

Dio3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.By similarity

Catalytic activityi

3,3',5'-triiodo-L-thyronine + iodide + acceptor + H+ = L-thyroxine + reduced acceptor.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1701

GO - Molecular functioni

  • thyroxine 5'-deiodinase activity Source: MGI
  • thyroxine 5-deiodinase activity Source: UniProtKB

GO - Biological processi

  • hormone biosynthetic process Source: UniProtKB-KW
  • positive regulation of multicellular organism growth Source: MGI
  • thyroid hormone catabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Thyroid hormones biosynthesis

Enzyme and pathway databases

BRENDAi1.97.1.11. 3474.
ReactomeiR-MMU-350864. Regulation of thyroid hormone activity.

Names & Taxonomyi

Protein namesi
Recommended name:
Thyroxine 5-deiodinase (EC:1.21.99.3)
Alternative name(s):
5DIII
DIOIII
Type 3 DI
Type III iodothyronine deiodinase
Gene namesi
Name:Dio3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1306782. Dio3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 44CytoplasmicSequence analysisAdd BLAST44
Transmembranei45 – 67Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini68 – 304ExtracellularSequence analysisAdd BLAST237

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001543241 – 304Thyroxine 5-deiodinaseAdd BLAST304

Proteomic databases

PaxDbiQ91ZI8.
PRIDEiQ91ZI8.

PTM databases

PhosphoSitePlusiQ91ZI8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000075707.
CleanExiMM_DIO3.
ExpressionAtlasiQ91ZI8. baseline and differential.

Interactioni

Subunit structurei

Homodimer. May undergo minor heretodimerization with DIO1 and DIO2 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000133920.

Structurei

Secondary structure

1304
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi120 – 122Combined sources3
Beta strandi139 – 141Combined sources3
Turni143 – 145Combined sources3
Beta strandi148 – 150Combined sources3
Helixi151 – 154Combined sources4
Beta strandi161 – 166Combined sources6
Helixi171 – 175Combined sources5
Helixi177 – 187Combined sources11
Turni188 – 190Combined sources3
Beta strandi192 – 197Combined sources6
Beta strandi206 – 208Combined sources3
Helixi222 – 234Combined sources13
Beta strandi241 – 244Combined sources4
Helixi249 – 253Combined sources5
Beta strandi260 – 265Combined sources6
Beta strandi268 – 272Combined sources5
Helixi280 – 297Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TR3X-ray1.90A120-304[»]
4TR4X-ray1.93A120-304[»]
ProteinModelPortaliQ91ZI8.
SMRiQ91ZI8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the iodothyronine deiodinase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IZDN. Eukaryota.
ENOG4112999. LUCA.
GeneTreeiENSGT00820000127068.
HOGENOMiHOG000007088.
HOVERGENiHBG000099.
InParanoidiQ91ZI8.
KOiK07754.
OMAiDFLCIRR.
OrthoDBiEOG091G0V5C.
TreeFamiTF329721.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000643. Iodothyronine_deiodinase.
IPR008261. Iodothyronine_deiodinase_AS.
IPR027252. Iodothyronine_deiodinase_I/III.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11781. PTHR11781. 1 hit.
PfamiPF00837. T4_deiodinase. 1 hit.
[Graphical view]
PIRSFiPIRSF001330. IOD. 1 hit.
PIRSF500144. IODI_III. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS01205. T4_DEIODINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91ZI8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRQAASRLV VGEGEGPPGA SGPAATMLRS LLLHSLRLCA QTASCLVLFP
60 70 80 90 100
RFLGTAFMLW LLDFLCIRKH FLRRRHPDHP EPEVELNSEG EEMPPDDPPI
110 120 130 140 150
CVSDDNRLCT LASLKAVWHG QKLDFFKQAH EGGPAPNSEV VRPDGFQSQR
160 170 180 190 200
ILDYAQGTRP LVLNFGSCTU PPFMARMSAF QRLVTKYQRD VDFLIIYIEE
210 220 230 240 250
AHPSDGWVTT DSPYVIPQHR SLEDRVSAAR VLQQGAPGCA LVLDTMANSS
260 270 280 290 300
SSAYGAYFER LYVIQSGTIM YQGGRGPDGY QVSELRTWLE RYDEQLHGTR

PHRF
Length:304
Mass (Da):34,111
Last modified:May 14, 2014 - v4
Checksum:i877DD709B4104A15
GO

Sequence cautioni

The sequence AAI06848 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAI06849 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAL23960 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE24483 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAE26869 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Non-standard residue

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-standard residuei170Selenocysteine1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF426023 Genomic DNA. Translation: AAL23960.1. Different initiation.
AK140797 mRNA. Translation: BAE24483.1. Different initiation.
AK146060 mRNA. Translation: BAE26869.1. Different initiation.
AL591207 Genomic DNA. No translation available.
BC106847 mRNA. Translation: AAI06848.1. Different initiation.
BC106848 mRNA. Translation: AAI06849.1. Different initiation.
CCDSiCCDS26171.2.
RefSeqiNP_742117.2. NM_172119.2.
UniGeneiMm.154427.

Genome annotation databases

EnsembliENSMUST00000173014; ENSMUSP00000133920; ENSMUSG00000075707.
GeneIDi107585.
KEGGimmu:107585.
UCSCiuc007pbi.1. mouse.

Keywords - Coding sequence diversityi

Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF426023 Genomic DNA. Translation: AAL23960.1. Different initiation.
AK140797 mRNA. Translation: BAE24483.1. Different initiation.
AK146060 mRNA. Translation: BAE26869.1. Different initiation.
AL591207 Genomic DNA. No translation available.
BC106847 mRNA. Translation: AAI06848.1. Different initiation.
BC106848 mRNA. Translation: AAI06849.1. Different initiation.
CCDSiCCDS26171.2.
RefSeqiNP_742117.2. NM_172119.2.
UniGeneiMm.154427.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4TR3X-ray1.90A120-304[»]
4TR4X-ray1.93A120-304[»]
ProteinModelPortaliQ91ZI8.
SMRiQ91ZI8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000133920.

PTM databases

PhosphoSitePlusiQ91ZI8.

Proteomic databases

PaxDbiQ91ZI8.
PRIDEiQ91ZI8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000173014; ENSMUSP00000133920; ENSMUSG00000075707.
GeneIDi107585.
KEGGimmu:107585.
UCSCiuc007pbi.1. mouse.

Organism-specific databases

CTDi1735.
MGIiMGI:1306782. Dio3.

Phylogenomic databases

eggNOGiENOG410IZDN. Eukaryota.
ENOG4112999. LUCA.
GeneTreeiENSGT00820000127068.
HOGENOMiHOG000007088.
HOVERGENiHBG000099.
InParanoidiQ91ZI8.
KOiK07754.
OMAiDFLCIRR.
OrthoDBiEOG091G0V5C.
TreeFamiTF329721.

Enzyme and pathway databases

BRENDAi1.97.1.11. 3474.
ReactomeiR-MMU-350864. Regulation of thyroid hormone activity.

Miscellaneous databases

PROiQ91ZI8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000075707.
CleanExiMM_DIO3.
ExpressionAtlasiQ91ZI8. baseline and differential.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000643. Iodothyronine_deiodinase.
IPR008261. Iodothyronine_deiodinase_AS.
IPR027252. Iodothyronine_deiodinase_I/III.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11781. PTHR11781. 1 hit.
PfamiPF00837. T4_deiodinase. 1 hit.
[Graphical view]
PIRSFiPIRSF001330. IOD. 1 hit.
PIRSF500144. IODI_III. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS01205. T4_DEIODINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIOD3_MOUSE
AccessioniPrimary (citable) accession number: Q91ZI8
Secondary accession number(s): G3UY28, Q3UKD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: May 14, 2014
Last modified: November 2, 2016
This is version 132 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-27 is the initiator.Curated

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.