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Protein

Phospholipase ABHD3

Gene

Abhd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phospholipase that may play a role in phospholipids remodeling. May selectively cleave myristate (C14)-containing phosphatidylcholines through its predominant phospholipase 1 activity, cleaving preferentially acyl groups in sn1 position. In parallel, may have a minor phospholipase 2 activity acting on acyl groups in position sn2. In addition to (C14)-containing phosphatidylcholines, may also act on other medium-chain-containing and oxidatively truncated phospholipids.1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.By similarity
Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei220 – 2201Charge relay systemBy similarity
Active sitei346 – 3461Charge relay systemBy similarity
Active sitei375 – 3751Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

  • phosphatidylcholine metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Keywords - Biological processi

Lipid metabolism, Phospholipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-1483191. Synthesis of PC.

Protein family/group databases

ESTHERimouse-abhd3. abh_upf0017.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase ABHD3Curated (EC:3.1.1.32By similarity, EC:3.1.1.4By similarity)
Alternative name(s):
Abhydrolase domain-containing protein 3Imported
Lung alpha/beta hydrolase 31 Publication
Short name:
MmLABH31 Publication
Gene namesi
Name:Abhd3Imported
Synonyms:Labh31 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2147183. Abhd3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei25 – 4521Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice lacking Abhd3 are viable, fertile and have normal behavior.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Phospholipase ABHD3PRO_0000280209Add
BLAST

Proteomic databases

EPDiQ91ZH7.
MaxQBiQ91ZH7.
PaxDbiQ91ZH7.
PRIDEiQ91ZH7.

PTM databases

iPTMnetiQ91ZH7.
PhosphoSiteiQ91ZH7.

Expressioni

Tissue specificityi

Widely expressed with higher expression in liver.1 Publication

Developmental stagei

Detected in embryos from E7 to E17.1 Publication

Gene expression databases

BgeeiQ91ZH7.
CleanExiMM_ABHD3.
ExpressionAtlasiQ91ZH7. baseline and differential.
GenevisibleiQ91ZH7. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000002549.

Structurei

3D structure databases

ProteinModelPortaliQ91ZH7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1838. Eukaryota.
COG0429. LUCA.
GeneTreeiENSGT00530000062981.
HOGENOMiHOG000230596.
HOVERGENiHBG053994.
InParanoidiQ91ZH7.
KOiK13696.
OMAiILHMVQQ.
PhylomeDBiQ91ZH7.
TreeFamiTF313195.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000952. AB_hydrolase_4_CS.
IPR012020. ABHD4.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005211. Ab_hydro_YheT. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS01133. UPF0017. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91ZH7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQRLAMDLRV LSRELALYLE HQVRVGFFGS GVGLSLILGF SVAYACYYLS
60 70 80 90 100
SIAKKPQLVI GGESFSRFLQ DHCPVVTETY YPTVWCWESR GQTLLRPFIT
110 120 130 140 150
SKPPVQYRNE LIKTADGGQI SLDWFDNNNS AYYVDASTRP TILLLPGLTG
160 170 180 190 200
TSKESYILHM IHLSEELGYR CVVFNNRGVA GESLLTPRTY CCANTEDLEA
210 220 230 240 250
VVHHVHSLYP GAPFLAAGVS MGGMLLLNYL GKIGSKTPLM AAATFSVGWN
260 270 280 290 300
TFACSESLER PLNWLLFNYY LTTCLQSSVK KHRHMFVEQI DMDQVMKAKS
310 320 330 340 350
IREFDKRFTA VMFGYRTLDD YYTDASPNRR LKSVGIPVLC LNATDDVFSP
360 370 380 390 400
SHAIPIETAK QNPNVALVLT AYGGHIGFLE GIWPRQCTYM DRVFKQFVQA
410
MVEHGHELSN M
Length:411
Mass (Da):46,232
Last modified:December 1, 2001 - v1
Checksum:iA9B89D164214CC24
GO
Isoform 2 (identifier: Q91ZH7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     353-356: Missing.

Note: No experimental confirmation available.
Show »
Length:407
Mass (Da):45,838
Checksum:i39B7DBBC390C723D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 881E → A in BAE25790 (PubMed:16141072).Curated
Sequence conflicti169 – 1691Y → H in BAE25790 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei353 – 3564Missing in isoform 2. 1 PublicationVSP_023564

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF429302 mRNA. Translation: AAL27565.1.
AK134676 mRNA. Translation: BAE22236.1.
AK144238 mRNA. Translation: BAE25790.1.
BC026770 mRNA. Translation: AAH26770.1.
CCDSiCCDS29057.1. [Q91ZH7-1]
RefSeqiNP_598891.1. NM_134130.1. [Q91ZH7-1]
UniGeneiMm.273108.

Genome annotation databases

EnsembliENSMUST00000002549; ENSMUSP00000002549; ENSMUSG00000002475. [Q91ZH7-1]
GeneIDi106861.
KEGGimmu:106861.
UCSCiuc008eba.1. mouse. [Q91ZH7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF429302 mRNA. Translation: AAL27565.1.
AK134676 mRNA. Translation: BAE22236.1.
AK144238 mRNA. Translation: BAE25790.1.
BC026770 mRNA. Translation: AAH26770.1.
CCDSiCCDS29057.1. [Q91ZH7-1]
RefSeqiNP_598891.1. NM_134130.1. [Q91ZH7-1]
UniGeneiMm.273108.

3D structure databases

ProteinModelPortaliQ91ZH7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000002549.

Protein family/group databases

ESTHERimouse-abhd3. abh_upf0017.

PTM databases

iPTMnetiQ91ZH7.
PhosphoSiteiQ91ZH7.

Proteomic databases

EPDiQ91ZH7.
MaxQBiQ91ZH7.
PaxDbiQ91ZH7.
PRIDEiQ91ZH7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002549; ENSMUSP00000002549; ENSMUSG00000002475. [Q91ZH7-1]
GeneIDi106861.
KEGGimmu:106861.
UCSCiuc008eba.1. mouse. [Q91ZH7-1]

Organism-specific databases

CTDi171586.
MGIiMGI:2147183. Abhd3.

Phylogenomic databases

eggNOGiKOG1838. Eukaryota.
COG0429. LUCA.
GeneTreeiENSGT00530000062981.
HOGENOMiHOG000230596.
HOVERGENiHBG053994.
InParanoidiQ91ZH7.
KOiK13696.
OMAiILHMVQQ.
PhylomeDBiQ91ZH7.
TreeFamiTF313195.

Enzyme and pathway databases

ReactomeiR-MMU-1483191. Synthesis of PC.

Miscellaneous databases

NextBioi358434.
PROiQ91ZH7.
SOURCEiSearch...

Gene expression databases

BgeeiQ91ZH7.
CleanExiMM_ABHD3.
ExpressionAtlasiQ91ZH7. baseline and differential.
GenevisibleiQ91ZH7. MM.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000952. AB_hydrolase_4_CS.
IPR012020. ABHD4.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005211. Ab_hydro_YheT. 1 hit.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS01133. UPF0017. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and tissue distribution of three murine alpha/beta hydrolase fold protein cDNAs."
    Edgar A.J., Polak J.M.
    Biochem. Biophys. Res. Commun. 292:617-625(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: BALB/cJ.
    Tissue: Lung.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Medulla oblongata.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Kidney.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain and Liver.
  5. "Metabolomics annotates ABHD3 as a physiologic regulator of medium-chain phospholipids."
    Long J.Z., Cisar J.S., Milliken D., Niessen S., Wang C., Trauger S.A., Siuzdak G., Cravatt B.F.
    Nat. Chem. Biol. 7:763-765(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiABHD3_MOUSE
AccessioniPrimary (citable) accession number: Q91ZH7
Secondary accession number(s): Q3UNF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.