Q91ZE9 (BMF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bcl-2-modifying factor

Gene names

Name:

Bmf

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in apoptosis.
Subunit structure	Interacts with MCL1, BCL2, BCL2L1/BCL-Xl, BCL2A1 and BCL2L2/BCL-w. Interacts with the myosin V actin motor complex through its binding to DLC2. Ref.1
Tissue specificity	Widely expressed with an abundant expression in pancreas, liver kidney and hematopoietic tissues. Ref.4
Sequence similarities	Belongs to the Bcl-2 family.
Sequence caution	The sequence BAC39243.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Apoptosis
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from direct assay Ref.1. Source: UniProtKB positive regulation of protein homooligomerization Inferred from direct assay. Source: BHF-UCL positive regulation of release of cytochrome c from mitochondria Inferred from genetic interaction. Source: BHF-UCL
Cellular component	myosin complex Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction Ref.1. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCL2	P10415	2	EBI-708032,EBI-77694	From a different organism.
BCL2L2	Q92843	2	EBI-708032,EBI-707714	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 185

185

Bcl-2-modifying factor

PRO_0000143112

Regions

Region

67 – 75

9

Interaction with DLC2

Motif

134 – 148

15

BH3

Experimental info

Mutagenesis

67

1

D → A: Loss of interaction with DLC2. Ref.1

Mutagenesis

68

1

K → A: Loss of interaction with DLC2. Ref.1

Mutagenesis

69

1

A → P: Loss of interaction with DLC2. Ref.1

Mutagenesis

138

1

L → A: Decrease in the interaction with BCL2 and BCL2L2.

Secondary structure

1

.

.

.

185

Helix Strand Turn

Details...

Helix

130 – 149

20

Sequences

	Sequence		Length	Mass (Da)
	Q91ZE9 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: DAE6EA080F3EA06B	FASTA	185	20,682
	10 20 30 40 50 60 MEPPQCVEEL EDDVFQSEDG EPGTQPGGLL SADLFAQSQL DCPLSRLQLF PLTHCCGPGL 70 80 90 100 110 120 RPISQEDKAT QTLSPASPSQ GVMLPCGVTE EPQRLFYGNA GYRLPLPASF PAGSPLGEQP 130 140 150 160 170 180 PEGQFLQHRA EVQIARKLQC IADQFHRLHT QQHQQNRDRA WWQVFLFLQN LALNRQENRE GVGPW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis." Puthalakath H., Villunger A., O'Reilly L.A., Beaumont J.G., Coultas L., Cheney R.E., Huang D.C.S., Strasser A. Science 293:1829-1832(2001) [PubMed: 11546872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH MCL1; BCL2; BCL2L1; BCL2L2 AND DLC2, MUTAGENESIS OF ASP-67; LYS-68 AND ALA-69. Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Heart and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"Expression and transcriptional regulation of functionally distinct Bmf isoforms in B-chronic lymphocytic leukemia cells." Morales A.A., Olsson A., Celsing F., Oesterborg A., Jondal M., Osorio L.M. Leukemia 18:41-47(2004) [PubMed: 14574334] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Structural plasticity underpins promiscuous binding of the prosurvival protein A1." Smits C., Czabotar P.E., Hinds M.G., Day C.L. Structure 16:818-829(2008) [PubMed: 18462686] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 126-152 IN COMPLEX WITH BCL2A1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY029253 mRNA. Translation: AAK38747.1.
AK084658 mRNA. Translation: BAC39243.1. Different initiation.
AK040622 mRNA. Translation: BAC30649.1.
BC079650 mRNA. Translation: AAH79650.1.

IPI

IPI00624659.

RefSeq

NP_612186.2. NM_138313.3.

UniGene

Mm.210125.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VOG	X-ray	1.90	B	126-152	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29806N.

IntAct

Q91ZE9. 5 interactions.

MINT

MINT-209586.

STRING

Q91ZE9.

PTM databases

PhosphoSite

Q91ZE9.

Proteomic databases

PRIDE

Q91ZE9.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

171543.

KEGG

mmu:171543.

Organism-specific databases

CTD

90427.

MGI

MGI:2176433. Bmf.

Phylogenomic databases

GeneTree

ENSGT00390000017896.

HOVERGEN

HBG050697.

InParanoid

Q91ZE9.

OrthoDB

EOG4FR0SM.

Gene expression databases

ArrayExpress

Q91ZE9.

Bgee

Q91ZE9.

CleanEx

MM_BMF.

Genevestigator

Q91ZE9.

GermOnline

ENSMUSG00000040093. Mus musculus.

Family and domain databases

PROSITE

PS50062. BCL2_FAMILY. False negative.
PS01259. BH3. False negative.
[Graphical view]

ProtoNet

Search...

Other

NextBio

371060.

SOURCE

Search...

Entry information

Entry name

BMF_MOUSE

Accession

Primary (citable) accession number: Q91ZE9
Secondary accession number(s): Q8BUK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	December 1, 2001
Last modified:	September 21, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program