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Protein

Bcl-2-modifying factor

Gene

Bmf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in apoptosis.

GO - Biological processi

  • anoikis Source: MGI
  • negative regulation of apoptotic signaling pathway Source: MGI
  • positive regulation of apoptotic signaling pathway Source: MGI
  • positive regulation of protein homooligomerization Source: BHF-UCL
  • positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl-2-modifying factor
Gene namesi
Name:Bmf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:2176433. Bmf.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • cytoplasm Source: MGI
  • myosin complex Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi67 – 671D → A: Loss of interaction with DLC2. 1 Publication
Mutagenesisi68 – 681K → A: Loss of interaction with DLC2. 1 Publication
Mutagenesisi69 – 691A → P: Loss of interaction with DLC2. 1 Publication
Mutagenesisi138 – 1381L → A: Decrease in the interaction with BCL2 and BCL2L2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185Bcl-2-modifying factorPRO_0000143112Add
BLAST

Proteomic databases

PaxDbiQ91ZE9.
PRIDEiQ91ZE9.

PTM databases

iPTMnetiQ91ZE9.
PhosphoSiteiQ91ZE9.

Expressioni

Tissue specificityi

Widely expressed with an abundant expression in pancreas, liver kidney and hematopoietic tissues.1 Publication

Gene expression databases

BgeeiQ91ZE9.
CleanExiMM_BMF.

Interactioni

Subunit structurei

Interacts with MCL1, BCL2, BCL2L1/BCL-Xl, BCL2A1 and BCL2L2/BCL-w. Interacts with the myosin V actin motor complex through its binding to DLC2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BCL2P104152EBI-708032,EBI-77694From a different organism.
BCL2L2Q928432EBI-708032,EBI-707714From a different organism.

Protein-protein interaction databases

DIPiDIP-29806N.
IntActiQ91ZE9. 5 interactions.
MINTiMINT-209586.
STRINGi10090.ENSMUSP00000087686.

Structurei

Secondary structure

1
185
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi130 – 14920Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VOGX-ray1.90B126-152[»]
DisProtiDP00645.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91ZE9.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 759Interaction with DLC2

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi134 – 14815BH3Add
BLAST

Sequence similaritiesi

Belongs to the Bcl-2 family.Curated

Phylogenomic databases

eggNOGiENOG410IHFT. Eukaryota.
ENOG410YIXY. LUCA.
HOVERGENiHBG050697.
InParanoidiQ91ZE9.
KOiK17460.

Family and domain databases

InterProiIPR028192. BMF.
[Graphical view]
PANTHERiPTHR32014. PTHR32014. 1 hit.
PfamiPF15185. BMF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91ZE9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPPQCVEEL EDDVFQSEDG EPGTQPGGLL SADLFAQSQL DCPLSRLQLF
60 70 80 90 100
PLTHCCGPGL RPISQEDKAT QTLSPASPSQ GVMLPCGVTE EPQRLFYGNA
110 120 130 140 150
GYRLPLPASF PAGSPLGEQP PEGQFLQHRA EVQIARKLQC IADQFHRLHT
160 170 180
QQHQQNRDRA WWQVFLFLQN LALNRQENRE GVGPW
Length:185
Mass (Da):20,682
Last modified:December 1, 2001 - v1
Checksum:iDAE6EA080F3EA06B
GO

Sequence cautioni

The sequence BAC39243.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY029253 mRNA. Translation: AAK38747.1.
AK084658 mRNA. Translation: BAC39243.1. Different initiation.
AK040622 mRNA. Translation: BAC30649.1.
BC079650 mRNA. Translation: AAH79650.1.
RefSeqiNP_612186.2. NM_138313.3.
XP_006498918.1. XM_006498855.1.
XP_006498919.1. XM_006498856.2.
UniGeneiMm.210125.

Genome annotation databases

GeneIDi171543.
KEGGimmu:171543.
UCSCiuc008lsb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY029253 mRNA. Translation: AAK38747.1.
AK084658 mRNA. Translation: BAC39243.1. Different initiation.
AK040622 mRNA. Translation: BAC30649.1.
BC079650 mRNA. Translation: AAH79650.1.
RefSeqiNP_612186.2. NM_138313.3.
XP_006498918.1. XM_006498855.1.
XP_006498919.1. XM_006498856.2.
UniGeneiMm.210125.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VOGX-ray1.90B126-152[»]
DisProtiDP00645.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29806N.
IntActiQ91ZE9. 5 interactions.
MINTiMINT-209586.
STRINGi10090.ENSMUSP00000087686.

PTM databases

iPTMnetiQ91ZE9.
PhosphoSiteiQ91ZE9.

Proteomic databases

PaxDbiQ91ZE9.
PRIDEiQ91ZE9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi171543.
KEGGimmu:171543.
UCSCiuc008lsb.1. mouse.

Organism-specific databases

CTDi90427.
MGIiMGI:2176433. Bmf.

Phylogenomic databases

eggNOGiENOG410IHFT. Eukaryota.
ENOG410YIXY. LUCA.
HOVERGENiHBG050697.
InParanoidiQ91ZE9.
KOiK17460.

Miscellaneous databases

EvolutionaryTraceiQ91ZE9.
PROiQ91ZE9.
SOURCEiSearch...

Gene expression databases

BgeeiQ91ZE9.
CleanExiMM_BMF.

Family and domain databases

InterProiIPR028192. BMF.
[Graphical view]
PANTHERiPTHR32014. PTHR32014. 1 hit.
PfamiPF15185. BMF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis."
    Puthalakath H., Villunger A., O'Reilly L.A., Beaumont J.G., Coultas L., Cheney R.E., Huang D.C.S., Strasser A.
    Science 293:1829-1832(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH MCL1; BCL2; BCL2L1; BCL2L2 AND DLC2, MUTAGENESIS OF ASP-67; LYS-68 AND ALA-69.
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Heart and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "Expression and transcriptional regulation of functionally distinct Bmf isoforms in B-chronic lymphocytic leukemia cells."
    Morales A.A., Olsson A., Celsing F., Oesterborg A., Jondal M., Osorio L.M.
    Leukemia 18:41-47(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Structural plasticity underpins promiscuous binding of the prosurvival protein A1."
    Smits C., Czabotar P.E., Hinds M.G., Day C.L.
    Structure 16:818-829(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 126-152 IN COMPLEX WITH BCL2A1.

Entry informationi

Entry nameiBMF_MOUSE
AccessioniPrimary (citable) accession number: Q91ZE9
Secondary accession number(s): Q8BUK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.