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Protein

Adhesion G protein-coupled receptor E4

Gene

Adgre4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May mediate the cellular interaction between myeloid cells and B-cells.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Ligandi

Calcium

Protein family/group databases

MEROPSiP02.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor E4
Alternative name(s):
EGF-like module receptor 4
EGF-like module-containing mucin-like hormone receptor-like 4
F4/80-like-receptor
Seven-span membrane protein FIRE
Gene namesi
Name:Adgre4
Synonyms:Emr4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1196464. Adgre4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini38 – 350ExtracellularSequence analysisAdd BLAST313
Transmembranei351 – 371Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini372 – 379CytoplasmicSequence analysis8
Transmembranei380 – 400Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini401 – 407ExtracellularSequence analysis7
Transmembranei408 – 428Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini429 – 455CytoplasmicSequence analysisAdd BLAST27
Transmembranei456 – 476Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini477 – 499ExtracellularSequence analysisAdd BLAST23
Transmembranei500 – 520Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini521 – 544CytoplasmicSequence analysisAdd BLAST24
Transmembranei545 – 565Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini566 – 570ExtracellularSequence analysis5
Transmembranei571 – 591Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini592 – 689CytoplasmicSequence analysisAdd BLAST98

GO - Cellular componenti

  • cell surface Source: MGI
  • integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Sequence analysisAdd BLAST37
ChainiPRO_000001287838 – 689Adhesion G protein-coupled receptor E4Add BLAST652

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi38 ↔ 47By similarity
Disulfide bondi41 ↔ 53By similarity
Glycosylationi44N-linked (GlcNAc...)Sequence analysis1
Glycosylationi49N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi55 ↔ 75By similarity
Disulfide bondi81 ↔ 94By similarity
Disulfide bondi88 ↔ 103By similarity
Disulfide bondi105 ↔ 126By similarity
Glycosylationi129N-linked (GlcNAc...)Sequence analysis1
Glycosylationi203N-linked (GlcNAc...)Sequence analysis1
Glycosylationi238N-linked (GlcNAc...)Sequence analysis1
Glycosylationi315N-linked (GlcNAc...)Sequence analysis1
Glycosylationi401N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.1 Publication
Glycosylated.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei326 – 327Cleavage1 Publication2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ91ZE5.
PRIDEiQ91ZE5.

PTM databases

PhosphoSitePlusiQ91ZE5.

Expressioni

Tissue specificityi

Predominantly expressed in myeloid cells. Predominantly expressed on resident macrophages.2 Publications

Inductioni

Up-regulated following macrophage activation.1 Publication

Gene expression databases

BgeeiENSMUSG00000032915.
GenevisibleiQ91ZE5. MM.

Interactioni

Subunit structurei

Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit).1 Publication

Protein-protein interaction databases

MINTiMINT-4997895.
STRINGi10090.ENSMUSP00000025004.

Structurei

3D structure databases

ProteinModelPortaliQ91ZE5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 76EGF-like 1Add BLAST39
Domaini77 – 127EGF-like 2; calcium-bindingSequence analysisAdd BLAST51
Domaini289 – 338GPSPROSITE-ProRule annotationAdd BLAST50

Domaini

The second EGF domain mediates the interaction with the putative ligand.1 Publication

Sequence similaritiesi

Contains 2 EGF-like domains.Curated
Contains 1 GPS domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOGENOMiHOG000294115.
HOVERGENiHBG048917.
InParanoidiQ91ZE5.
KOiK08445.
OMAiFVLGCSW.
OrthoDBiEOG091G0404.
PhylomeDBiQ91ZE5.
TreeFamiTF316380.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR018097. EGF_Ca-bd_CS.
IPR017981. GPCR_2-like.
IPR001740. GPCR_2_EMR1-like_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF07645. EGF_CA. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR01128. EMR1HORMONER.
PR00249. GPCRSECRETIN.
SMARTiSM00179. EGF_CA. 1 hit.
SM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91ZE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLMGATRDMG SRCLLHASVP GMLLIWSILQ MMNISASCPQ CNENASCFNS
60 70 80 90 100
THCVCKEGFW TGSENRRIIE PHEKCQDINE CLLKELVCKD VSYCRNKIGT
110 120 130 140 150
YICSCVVKYP LFNWVAGIIN IDHPDCYVNK SKNTGSKTHT LGVLSEFKSK
160 170 180 190 200
EEVAKGATKL LRKVEHHILN ENSDIPKKDE NPLLDIVYET KRCKTMTLLE
210 220 230 240 250
AGNNTMKVDC TSGFKEHNSG GETAVAFIAY KSLGNLLNGS FFSNEEGFQE
260 270 280 290 300
VTLNSHIVSG AIRSEVKPVL SEPVLLTLQN IQPIDSRAEH LCVHWEGSEE
310 320 330 340 350
GGSWSTKGCS HVYTNNSYTI CKCFHLSSFA VLMALPHEED GVLSALSVIT
360 370 380 390 400
YVGLSLSLLC LFLAAITFLL CRPIQNTSTT LHLQLSICLF LADLLFLTGI
410 420 430 440 450
NRTKPKVLCS IIAGMLHYLY LASFMWMFLE GLHLFLTVSN LKVANYSNSG
460 470 480 490 500
RFKKRFMYPV GYGLPAFIVA VSAIAGHKNY GTHNHCWLSL HRGFIWSFLG
510 520 530 540 550
PAAAIILINL VFYFLIIWIL RSKLSSLNKE VSTLQDTKVM TFKAIVQLFV
560 570 580 590 600
LGCSWGIGLF IFIEVGKTVR LIVAYLFTII NVLQGVLIFM VHCLLNRQVR
610 620 630 640 650
MEYKKWFHRL RKEVESESTE VSHSTTHTKM GLSLNLENFC PTGNLHDPSD
660 670 680
SILPSTEVAG VYLSTPRSHM GAEDVNSGTH AYWSRTISD
Length:689
Mass (Da):77,045
Last modified:December 1, 2001 - v1
Checksum:iD9469A095CBC2088
GO

Sequence cautioni

The sequence AAL31879 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti666P → H in BAC29816 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY032690 mRNA. Translation: AAK51125.1.
AF396935 mRNA. Translation: AAL31879.1. Different initiation.
AK037483 mRNA. Translation: BAC29816.1.
AK155935 mRNA. Translation: BAE33510.1.
CCDSiCCDS50148.1.
RefSeqiNP_631877.2. NM_139138.3.
UniGeneiMm.210497.

Genome annotation databases

EnsembliENSMUST00000025004; ENSMUSP00000025004; ENSMUSG00000032915.
GeneIDi52614.
KEGGimmu:52614.
UCSCiuc008dae.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY032690 mRNA. Translation: AAK51125.1.
AF396935 mRNA. Translation: AAL31879.1. Different initiation.
AK037483 mRNA. Translation: BAC29816.1.
AK155935 mRNA. Translation: BAE33510.1.
CCDSiCCDS50148.1.
RefSeqiNP_631877.2. NM_139138.3.
UniGeneiMm.210497.

3D structure databases

ProteinModelPortaliQ91ZE5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4997895.
STRINGi10090.ENSMUSP00000025004.

Protein family/group databases

MEROPSiP02.005.
GPCRDBiSearch...

PTM databases

PhosphoSitePlusiQ91ZE5.

Proteomic databases

PaxDbiQ91ZE5.
PRIDEiQ91ZE5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025004; ENSMUSP00000025004; ENSMUSG00000032915.
GeneIDi52614.
KEGGimmu:52614.
UCSCiuc008dae.2. mouse.

Organism-specific databases

CTDi52614.
MGIiMGI:1196464. Adgre4.

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOGENOMiHOG000294115.
HOVERGENiHBG048917.
InParanoidiQ91ZE5.
KOiK08445.
OMAiFVLGCSW.
OrthoDBiEOG091G0404.
PhylomeDBiQ91ZE5.
TreeFamiTF316380.

Miscellaneous databases

PROiQ91ZE5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032915.
GenevisibleiQ91ZE5. MM.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR018097. EGF_Ca-bd_CS.
IPR017981. GPCR_2-like.
IPR001740. GPCR_2_EMR1-like_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF07645. EGF_CA. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR01128. EMR1HORMONER.
PR00249. GPCRSECRETIN.
SMARTiSM00179. EGF_CA. 1 hit.
SM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGRE4_MOUSE
AccessioniPrimary (citable) accession number: Q91ZE5
Secondary accession number(s): Q3U1I7, Q8BYX0, Q8VIM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.