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Protein

Adhesion G protein-coupled receptor E4

Gene

Adgre4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could mediated the cellular interaction between myeloid cells and B-cells.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Ligandi

Calcium

Protein family/group databases

MEROPSiP02.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G protein-coupled receptor E4
Alternative name(s):
EGF-like module receptor 4
EGF-like module-containing mucin-like hormone receptor-like 4
F4/80-like-receptor
Seven-span membrane protein FIRE
Gene namesi
Name:Adgre4
Synonyms:Emr4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1196464. Adgre4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini38 – 350313ExtracellularSequence analysisAdd
BLAST
Transmembranei351 – 37121Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini372 – 3798CytoplasmicSequence analysis
Transmembranei380 – 40021Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini401 – 4077ExtracellularSequence analysis
Transmembranei408 – 42821Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini429 – 45527CytoplasmicSequence analysisAdd
BLAST
Transmembranei456 – 47621Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini477 – 49923ExtracellularSequence analysisAdd
BLAST
Transmembranei500 – 52021Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini521 – 54424CytoplasmicSequence analysisAdd
BLAST
Transmembranei545 – 56521Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini566 – 5705ExtracellularSequence analysis
Transmembranei571 – 59121Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini592 – 68998CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell surface Source: MGI
  • integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3737Sequence analysisAdd
BLAST
Chaini38 – 689652Adhesion G protein-coupled receptor E4PRO_0000012878Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence analysis
Disulfide bondi38 ↔ 47By similarity
Disulfide bondi41 ↔ 53By similarity
Glycosylationi44 – 441N-linked (GlcNAc...)Sequence analysis
Glycosylationi49 – 491N-linked (GlcNAc...)Sequence analysis
Disulfide bondi55 ↔ 75By similarity
Disulfide bondi81 ↔ 94By similarity
Disulfide bondi88 ↔ 103By similarity
Disulfide bondi105 ↔ 126By similarity
Glycosylationi129 – 1291N-linked (GlcNAc...)Sequence analysis
Glycosylationi203 – 2031N-linked (GlcNAc...)Sequence analysis
Glycosylationi238 – 2381N-linked (GlcNAc...)Sequence analysis
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence analysis
Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit.1 Publication
Glycosylated.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei326 – 3272Cleavage1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ91ZE5.
PaxDbiQ91ZE5.
PRIDEiQ91ZE5.

PTM databases

PhosphoSiteiQ91ZE5.

Expressioni

Tissue specificityi

Predominantly expressed in myeloid cells. Predominantly expressed on resident macrophages.2 Publications

Inductioni

Up-regulated following macrophage activation.1 Publication

Gene expression databases

BgeeiQ91ZE5.
GenevisibleiQ91ZE5. MM.

Interactioni

Subunit structurei

Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit).1 Publication

Protein-protein interaction databases

MINTiMINT-4997895.
STRINGi10090.ENSMUSP00000025004.

Structurei

3D structure databases

ProteinModelPortaliQ91ZE5.
SMRiQ91ZE5. Positions 41-134, 215-336, 438-596.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 7639EGF-like 1Add
BLAST
Domaini77 – 12751EGF-like 2; calcium-bindingSequence analysisAdd
BLAST
Domaini289 – 33850GPSPROSITE-ProRule annotationAdd
BLAST

Domaini

The second EGF domain mediates the interaction with the putative ligand.1 Publication

Sequence similaritiesi

Contains 2 EGF-like domains.Curated
Contains 1 GPS domain.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOGENOMiHOG000294115.
HOVERGENiHBG048917.
InParanoidiQ91ZE5.
KOiK08445.
OMAiFVLGCSW.
OrthoDBiEOG7Z0JW1.
PhylomeDBiQ91ZE5.
TreeFamiTF316380.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR018097. EGF_Ca-bd_CS.
IPR017981. GPCR_2-like.
IPR001740. GPCR_2_EMR1-like_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF07645. EGF_CA. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR01128. EMR1HORMONER.
PR00249. GPCRSECRETIN.
SMARTiSM00179. EGF_CA. 1 hit.
SM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91ZE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLMGATRDMG SRCLLHASVP GMLLIWSILQ MMNISASCPQ CNENASCFNS
60 70 80 90 100
THCVCKEGFW TGSENRRIIE PHEKCQDINE CLLKELVCKD VSYCRNKIGT
110 120 130 140 150
YICSCVVKYP LFNWVAGIIN IDHPDCYVNK SKNTGSKTHT LGVLSEFKSK
160 170 180 190 200
EEVAKGATKL LRKVEHHILN ENSDIPKKDE NPLLDIVYET KRCKTMTLLE
210 220 230 240 250
AGNNTMKVDC TSGFKEHNSG GETAVAFIAY KSLGNLLNGS FFSNEEGFQE
260 270 280 290 300
VTLNSHIVSG AIRSEVKPVL SEPVLLTLQN IQPIDSRAEH LCVHWEGSEE
310 320 330 340 350
GGSWSTKGCS HVYTNNSYTI CKCFHLSSFA VLMALPHEED GVLSALSVIT
360 370 380 390 400
YVGLSLSLLC LFLAAITFLL CRPIQNTSTT LHLQLSICLF LADLLFLTGI
410 420 430 440 450
NRTKPKVLCS IIAGMLHYLY LASFMWMFLE GLHLFLTVSN LKVANYSNSG
460 470 480 490 500
RFKKRFMYPV GYGLPAFIVA VSAIAGHKNY GTHNHCWLSL HRGFIWSFLG
510 520 530 540 550
PAAAIILINL VFYFLIIWIL RSKLSSLNKE VSTLQDTKVM TFKAIVQLFV
560 570 580 590 600
LGCSWGIGLF IFIEVGKTVR LIVAYLFTII NVLQGVLIFM VHCLLNRQVR
610 620 630 640 650
MEYKKWFHRL RKEVESESTE VSHSTTHTKM GLSLNLENFC PTGNLHDPSD
660 670 680
SILPSTEVAG VYLSTPRSHM GAEDVNSGTH AYWSRTISD
Length:689
Mass (Da):77,045
Last modified:December 1, 2001 - v1
Checksum:iD9469A095CBC2088
GO

Sequence cautioni

The sequence AAL31879.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti666 – 6661P → H in BAC29816 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY032690 mRNA. Translation: AAK51125.1.
AF396935 mRNA. Translation: AAL31879.1. Different initiation.
AK037483 mRNA. Translation: BAC29816.1.
AK155935 mRNA. Translation: BAE33510.1.
CCDSiCCDS50148.1.
RefSeqiNP_631877.2. NM_139138.3.
UniGeneiMm.210497.

Genome annotation databases

EnsembliENSMUST00000025004; ENSMUSP00000025004; ENSMUSG00000032915.
GeneIDi52614.
KEGGimmu:52614.
UCSCiuc008dae.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY032690 mRNA. Translation: AAK51125.1.
AF396935 mRNA. Translation: AAL31879.1. Different initiation.
AK037483 mRNA. Translation: BAC29816.1.
AK155935 mRNA. Translation: BAE33510.1.
CCDSiCCDS50148.1.
RefSeqiNP_631877.2. NM_139138.3.
UniGeneiMm.210497.

3D structure databases

ProteinModelPortaliQ91ZE5.
SMRiQ91ZE5. Positions 41-134, 215-336, 438-596.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4997895.
STRINGi10090.ENSMUSP00000025004.

Protein family/group databases

MEROPSiP02.005.
GPCRDBiSearch...

PTM databases

PhosphoSiteiQ91ZE5.

Proteomic databases

MaxQBiQ91ZE5.
PaxDbiQ91ZE5.
PRIDEiQ91ZE5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025004; ENSMUSP00000025004; ENSMUSG00000032915.
GeneIDi52614.
KEGGimmu:52614.
UCSCiuc008dae.2. mouse.

Organism-specific databases

CTDi52614.
MGIiMGI:1196464. Adgre4.

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOGENOMiHOG000294115.
HOVERGENiHBG048917.
InParanoidiQ91ZE5.
KOiK08445.
OMAiFVLGCSW.
OrthoDBiEOG7Z0JW1.
PhylomeDBiQ91ZE5.
TreeFamiTF316380.

Miscellaneous databases

PROiQ91ZE5.
SOURCEiSearch...

Gene expression databases

BgeeiQ91ZE5.
GenevisibleiQ91ZE5. MM.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR018097. EGF_Ca-bd_CS.
IPR017981. GPCR_2-like.
IPR001740. GPCR_2_EMR1-like_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF07645. EGF_CA. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR01128. EMR1HORMONER.
PR00249. GPCRSECRETIN.
SMARTiSM00179. EGF_CA. 1 hit.
SM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS01187. EGF_CA. 1 hit.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "EMR4, a novel epidermal growth factor (EGF)-TM7 molecule up-regulated in activated mouse macrophages, binds to a putative cellular ligand on B lymphoma cell line A20."
    Stacey M., Chang G.-W., Sanos S.L., Chittenden L.R., Stubbs L., Gordon S., Lin H.-H.
    J. Biol. Chem. 277:29283-29293(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 327-336, FUNCTION, TISSUE SPECIFICITY, INDUCTION, GLYCOSYLATION.
    Strain: BALB/cJ.
  2. "Molecular cloning of F4/80-like-receptor, a seven-span membrane protein expressed differentially by dendritic cell and monocyte-macrophage subpopulations."
    Caminschi I., Lucas K.M., O'Keeffe M.A., Hochrein H., Laabi Y., Koentgen F., Lew A.M., Shortman K., Wright M.D.
    J. Immunol. 167:3570-3576(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/6J.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Spleen and Thymus.

Entry informationi

Entry nameiAGRE4_MOUSE
AccessioniPrimary (citable) accession number: Q91ZE5
Secondary accession number(s): Q3U1I7, Q8BYX0, Q8VIM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: December 1, 2001
Last modified: June 8, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.