Q91ZE5 (EMR4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
EGF-like module-containing mucin-like hormone receptor-like 4

Alternative name(s):
EGF-like module receptor 4
F4/80-like-receptor
Seven-span membrane protein FIRE

Gene names

Name:

Emr4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Could mediated the cellular interaction between myeloid cells and B-cells.
Subunit structure	Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Predominantly expressed in myeloid cells.
Domain	The second EGF domain mediates the interaction with the putative ligand.
Post-translational modification	Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. Contains 2 EGF-like domains. Contains 1 GPS domain.
Sequence caution	The sequence AAL31879.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	EGF-like domain Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	neuropeptide signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	cell surface Inferred from direct assay Ref.1. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 37	37	Potential
	Chain	38 – 689	652	EGF-like module-containing mucin-like hormone receptor-like 4		PRO_0000012878
Regions
	Topological domain	38 – 350	313	Extracellular Potential
	Transmembrane	351 – 371	21	Helical; Name=1; Potential
	Topological domain	372 – 379	8	Cytoplasmic Potential
	Transmembrane	380 – 400	21	Helical; Name=2; Potential
	Topological domain	401 – 407	7	Extracellular Potential
	Transmembrane	408 – 428	21	Helical; Name=3; Potential
	Topological domain	429 – 455	27	Cytoplasmic Potential
	Transmembrane	456 – 476	21	Helical; Name=4; Potential
	Topological domain	477 – 499	23	Extracellular Potential
	Transmembrane	500 – 520	21	Helical; Name=5; Potential
	Topological domain	521 – 544	24	Cytoplasmic Potential
	Transmembrane	545 – 565	21	Helical; Name=6; Potential
	Topological domain	566 – 570	5	Extracellular Potential
	Transmembrane	571 – 591	21	Helical; Name=7; Potential
	Topological domain	592 – 689	98	Cytoplasmic Potential
	Domain	38 – 76	39	EGF-like 1
	Domain	77 – 127	51	EGF-like 2; calcium-binding Potential
	Domain	289 – 338	50	GPS
Sites
	Site	326 – 327	2	Cleavage
Amino acid modifications
	Glycosylation	33	1	N-linked (GlcNAc...) Potential
	Glycosylation	44	1	N-linked (GlcNAc...) Potential
	Glycosylation	49	1	N-linked (GlcNAc...) Potential
	Glycosylation	129	1	N-linked (GlcNAc...) Potential
	Glycosylation	203	1	N-linked (GlcNAc...) Potential
	Glycosylation	238	1	N-linked (GlcNAc...) Potential
	Glycosylation	315	1	N-linked (GlcNAc...) Potential
	Glycosylation	401	1	N-linked (GlcNAc...) Potential
	Disulfide bond	38 ↔ 47		By similarity
	Disulfide bond	41 ↔ 53		By similarity
	Disulfide bond	55 ↔ 75		By similarity
	Disulfide bond	81 ↔ 94		By similarity
	Disulfide bond	88 ↔ 103		By similarity
	Disulfide bond	105 ↔ 126		By similarity
Experimental info
	Sequence conflict	666	1	P → H in BAC29816. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q91ZE5 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: D9469A095CBC2088	FASTA	689	77,045
	10 20 30 40 50 60 MLMGATRDMG SRCLLHASVP GMLLIWSILQ MMNISASCPQ CNENASCFNS THCVCKEGFW 70 80 90 100 110 120 TGSENRRIIE PHEKCQDINE CLLKELVCKD VSYCRNKIGT YICSCVVKYP LFNWVAGIIN 130 140 150 160 170 180 IDHPDCYVNK SKNTGSKTHT LGVLSEFKSK EEVAKGATKL LRKVEHHILN ENSDIPKKDE 190 200 210 220 230 240 NPLLDIVYET KRCKTMTLLE AGNNTMKVDC TSGFKEHNSG GETAVAFIAY KSLGNLLNGS 250 260 270 280 290 300 FFSNEEGFQE VTLNSHIVSG AIRSEVKPVL SEPVLLTLQN IQPIDSRAEH LCVHWEGSEE 310 320 330 340 350 360 GGSWSTKGCS HVYTNNSYTI CKCFHLSSFA VLMALPHEED GVLSALSVIT YVGLSLSLLC 370 380 390 400 410 420 LFLAAITFLL CRPIQNTSTT LHLQLSICLF LADLLFLTGI NRTKPKVLCS IIAGMLHYLY 430 440 450 460 470 480 LASFMWMFLE GLHLFLTVSN LKVANYSNSG RFKKRFMYPV GYGLPAFIVA VSAIAGHKNY 490 500 510 520 530 540 GTHNHCWLSL HRGFIWSFLG PAAAIILINL VFYFLIIWIL RSKLSSLNKE VSTLQDTKVM 550 560 570 580 590 600 TFKAIVQLFV LGCSWGIGLF IFIEVGKTVR LIVAYLFTII NVLQGVLIFM VHCLLNRQVR 610 620 630 640 650 660 MEYKKWFHRL RKEVESESTE VSHSTTHTKM GLSLNLENFC PTGNLHDPSD SILPSTEVAG 670 680 VYLSTPRSHM GAEDVNSGTH AYWSRTISD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"EMR4, a novel epidermal growth factor (EGF)-TM7 molecule up-regulated in activated mouse macrophages, binds to a putative cellular ligand on B lymphoma cell line A20." Stacey M., Chang G.-W., Sanos S.L., Chittenden L.R., Stubbs L., Gordon S., Lin H.-H. J. Biol. Chem. 277:29283-29293(2002) [PubMed: 12023293] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 327-336. Strain: BALB/c.
[2]	"Molecular cloning of F4/80-like-receptor, a seven-span membrane protein expressed differentially by dendritic cell and monocyte-macrophage subpopulations." Caminschi I., Lucas K.M., O'Keeffe M.A., Hochrein H., Laabi Y., Koentgen F., Lew A.M., Shortman K., Wright M.D. J. Immunol. 167:3570-3576(2001) [PubMed: 11564768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Spleen and Thymus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY032690 mRNA. Translation: AAK51125.1. AF396935 mRNA. Translation: AAL31879.1. Different initiation. AK037483 mRNA. Translation: BAC29816.1. AK155935 mRNA. Translation: BAE33510.1.
IPI	IPI00131116.
RefSeq	NP_631877.2. NM_139138.3.
UniGene	Mm.210497.
3D structure databases
ProteinModelPortal	Q91ZE5.
SMR	Q91ZE5. Positions 9-112.
ModBase	Search...
Protein-protein interaction databases
STRING	Q91ZE5.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	Q91ZE5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000025004; ENSMUSP00000025004; ENSMUSG00000032915.
GeneID	52614.
KEGG	mmu:52614.
UCSC	uc008dae.2. mouse.
Organism-specific databases
CTD	52614.
MGI	MGI:1196464. Emr4.
Phylogenomic databases
GeneTree	ENSGT00600000084176.
HOGENOM	HBG506068.
HOVERGEN	HBG048917.
InParanoid	Q91ZE5.
OMA	KCNETSE.
OrthoDB	EOG4H463Q.
PhylomeDB	Q91ZE5.
Gene expression databases
ArrayExpress	Q91ZE5.
Bgee	Q91ZE5.
Genevestigator	Q91ZE5.
GermOnline	ENSMUSG00000032915. Mus musculus.
Family and domain databases
InterPro	IPR001881. EGF-like_Ca-bd. IPR018097. EGF_Ca-bd_CS. IPR017981. GPCR_2-like. IPR001740. GPCR_2_EMR1_rcpt. IPR000832. GPCR_2_secretin-like. IPR017983. GPCR_2_secretin-like_CS. IPR000203. GPS_dom. [Graphical view]
KO	K08445.
Pfam	PF00002. 7tm_2. 1 hit. PF07645. EGF_CA. 1 hit. PF01825. GPS. 1 hit. [Graphical view]
PRINTS	PR01128. EMR1HORMONER. PR00249. GPCRSECRETIN.
SMART	SM00303. GPS. 1 hit. [Graphical view]
PROSITE	PS00010. ASX_HYDROXYL. 1 hit. PS50026. EGF_3. False negative. PS01187. EGF_CA. 1 hit. PS00650. G_PROTEIN_RECEP_F2_2. 1 hit. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. PS50221. GPS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	309213.
SOURCE	Search...

Entry information

Entry name

EMR4_MOUSE

Accession

Primary (citable) accession number: Q91ZE5
Secondary accession number(s): Q3U1I7, Q8BYX0, Q8VIM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	December 1, 2001
Last modified:	November 16, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program