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Protein

ELL-associated factor 2

Gene

Eaf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional transactivator of ELL and ELL2 elongation activities (By similarity). Acts as a transcriptional transactivator of TCEA1 elongation activity.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • intrinsic apoptotic signaling pathway Source: MGI
  • negative regulation of cell growth Source: MGI
  • negative regulation of epithelial cell proliferation involved in prostate gland development Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-112382. Formation of RNA Pol II elongation complex.
R-MMU-112387. Elongation arrest and recovery.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-75955. RNA Polymerase II Transcription Elongation.

Names & Taxonomyi

Protein namesi
Recommended name:
ELL-associated factor 2
Alternative name(s):
Ehrlich S-II transcriptional activator factor
Testosterone-regulated apoptosis inducer and tumor suppressor protein
Gene namesi
Name:Eaf2
Synonyms:Festa, Traits
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2146616. Eaf2.

Subcellular locationi

  • Nucleus speckle By similarity

GO - Cellular componenti

  • ELL-EAF complex Source: InterPro
  • nuclear speck Source: UniProtKB-SubCell
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • transcription elongation factor complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001303381 – 262ELL-associated factor 2Add BLAST262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei146PhosphoserineBy similarity1
Modified residuei151PhosphoserineBy similarity1
Modified residuei154PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ91ZD6.
PRIDEiQ91ZD6.

PTM databases

PhosphoSitePlusiQ91ZD6.

Expressioni

Tissue specificityi

Isoform 1 is expressed in ovary, uterus, mammary glands, brain, spleen, liver, lung, thymus, kidney, skeletal muscle, skin and testis. Isoform 2 is expressed in kidney.2 Publications

Developmental stagei

Expressed in brain and spinal cord at 10 dpc. Expressed in brain, spinal cord, cranial and spinal ganglia, lens, retina, cochlea, olfactory epithelium and pituitary at 12 dpc. Expressed in intestine, bladder endothelium, retinal ganglion cells, nephrons, bronchial epithelium, secretory epithelium of submandibular glands, tubular epithelium of the epididymis, ectodermal invaginations of mammary buds and vibrissae follicles, incisors and molars at 15 dpc.1 Publication

Gene expression databases

BgeeiENSMUSG00000022838.
CleanExiMM_EAF2.
ExpressionAtlasiQ91ZD6. baseline and differential.
GenevisibleiQ91ZD6. MM.

Interactioni

Subunit structurei

Component of the super elongation complex (SEC), at least composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF (AFF1 or AFF4), the P-TEFb complex and ELL (ELL, ELL2 or ELL3). Interacts with ELL and ELL2 (By similarity). Isoform 1 and isoform 2 interact with TCEA1.By similarity1 Publication

Protein-protein interaction databases

BioGridi223046. 1 interactor.
IntActiQ91ZD6. 1 interactor.
STRINGi10090.ENSMUSP00000110477.

Structurei

3D structure databases

ProteinModelPortaliQ91ZD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni17 – 104Necessary for interaction with ELLBy similarityAdd BLAST88
Regioni177 – 262Necessary for transactivation activityBy similarityAdd BLAST86
Regioni248 – 262Necessary for interaction with TCEA1 and transactivation activity1 PublicationAdd BLAST15

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi126 – 130Poly-Gln5
Compositional biasi174 – 219Ser-richAdd BLAST46

Sequence similaritiesi

Belongs to the EAF family.Curated

Phylogenomic databases

eggNOGiKOG4795. Eukaryota.
ENOG4111KQJ. LUCA.
GeneTreeiENSGT00390000017724.
HOGENOMiHOG000070137.
HOVERGENiHBG054898.
InParanoidiQ91ZD6.
KOiK15186.
OMAiAPVTVFK.
OrthoDBiEOG091G0MMP.
PhylomeDBiQ91ZD6.
TreeFamiTF320864.

Family and domain databases

InterProiIPR027093. EAF_fam.
IPR019194. Tscrpt_elong_fac_Eaf_N.
[Graphical view]
PANTHERiPTHR15970. PTHR15970. 1 hit.
PfamiPF09816. EAF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91ZD6-1) [UniParc]FASTAAdd to basket
Also known as: FESTA-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGPAGLAYL DRRERVLKLG ESFEKQPRCA FHTVRYDFKP ASIDTSCEGN
60 70 80 90 100
LEVGKGEQVT ITLPNIEGST PPVTVFKGSK RPYLKECILI INHDTGECRL
110 120 130 140 150
EKLSSNITVK KTRVEGSSRI QYRLEQQQQQ MWNLPRTSNL VQHSPSEEKM
160 170 180 190 200
SPTSLMDDIE RELKAEASLM DQMSSCDSSS DSKSSSSSSS EDSSSDSEDD
210 220 230 240 250
DQFSPLGPRK YSSEHPSMSA GPQYRTSEAD ATCHRLQDHS TLLMSTLRSD
260
LQLSESESDS ED
Length:262
Mass (Da):29,187
Last modified:December 1, 2001 - v1
Checksum:i5D072D84816B766A
GO
Isoform 2 (identifier: Q91ZD6-2) [UniParc]FASTAAdd to basket
Also known as: FESTA-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-130: Missing.

Show »
Length:132
Mass (Da):14,534
Checksum:iEB80830042DBD1F2
GO

Sequence cautioni

The sequence AAH04721 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC77525 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0153111 – 130Missing in isoform 2. 2 PublicationsAdd BLAST130

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY049021 mRNA. Translation: AAL12224.1.
AY034479 mRNA. Translation: AAK59701.1.
AB081298 mRNA. Translation: BAC77525.1. Different initiation.
BC004721 mRNA. Translation: AAH04721.1. Different initiation.
BC056626 mRNA. Translation: AAH56626.1.
CCDSiCCDS37335.1. [Q91ZD6-2]
CCDS49843.1. [Q91ZD6-1]
RefSeqiNP_001106872.1. NM_001113401.1. [Q91ZD6-1]
NP_001106876.1. NM_001113405.1. [Q91ZD6-2]
NP_598872.1. NM_134111.2. [Q91ZD6-2]
XP_006521746.1. XM_006521683.1. [Q91ZD6-2]
XP_006521747.1. XM_006521684.3. [Q91ZD6-2]
XP_006521748.1. XM_006521685.3. [Q91ZD6-2]
XP_006521749.1. XM_006521686.2. [Q91ZD6-2]
UniGeneiMm.432078.

Genome annotation databases

EnsembliENSMUST00000075946; ENSMUSP00000075331; ENSMUSG00000022838. [Q91ZD6-2]
ENSMUST00000114825; ENSMUSP00000110473; ENSMUSG00000022838. [Q91ZD6-2]
ENSMUST00000114829; ENSMUSP00000110477; ENSMUSG00000022838. [Q91ZD6-1]
GeneIDi106389.
KEGGimmu:106389.
UCSCiuc007zcx.1. mouse. [Q91ZD6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY049021 mRNA. Translation: AAL12224.1.
AY034479 mRNA. Translation: AAK59701.1.
AB081298 mRNA. Translation: BAC77525.1. Different initiation.
BC004721 mRNA. Translation: AAH04721.1. Different initiation.
BC056626 mRNA. Translation: AAH56626.1.
CCDSiCCDS37335.1. [Q91ZD6-2]
CCDS49843.1. [Q91ZD6-1]
RefSeqiNP_001106872.1. NM_001113401.1. [Q91ZD6-1]
NP_001106876.1. NM_001113405.1. [Q91ZD6-2]
NP_598872.1. NM_134111.2. [Q91ZD6-2]
XP_006521746.1. XM_006521683.1. [Q91ZD6-2]
XP_006521747.1. XM_006521684.3. [Q91ZD6-2]
XP_006521748.1. XM_006521685.3. [Q91ZD6-2]
XP_006521749.1. XM_006521686.2. [Q91ZD6-2]
UniGeneiMm.432078.

3D structure databases

ProteinModelPortaliQ91ZD6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi223046. 1 interactor.
IntActiQ91ZD6. 1 interactor.
STRINGi10090.ENSMUSP00000110477.

PTM databases

PhosphoSitePlusiQ91ZD6.

Proteomic databases

PaxDbiQ91ZD6.
PRIDEiQ91ZD6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000075946; ENSMUSP00000075331; ENSMUSG00000022838. [Q91ZD6-2]
ENSMUST00000114825; ENSMUSP00000110473; ENSMUSG00000022838. [Q91ZD6-2]
ENSMUST00000114829; ENSMUSP00000110477; ENSMUSG00000022838. [Q91ZD6-1]
GeneIDi106389.
KEGGimmu:106389.
UCSCiuc007zcx.1. mouse. [Q91ZD6-1]

Organism-specific databases

CTDi55840.
MGIiMGI:2146616. Eaf2.

Phylogenomic databases

eggNOGiKOG4795. Eukaryota.
ENOG4111KQJ. LUCA.
GeneTreeiENSGT00390000017724.
HOGENOMiHOG000070137.
HOVERGENiHBG054898.
InParanoidiQ91ZD6.
KOiK15186.
OMAiAPVTVFK.
OrthoDBiEOG091G0MMP.
PhylomeDBiQ91ZD6.
TreeFamiTF320864.

Enzyme and pathway databases

ReactomeiR-MMU-112382. Formation of RNA Pol II elongation complex.
R-MMU-112387. Elongation arrest and recovery.
R-MMU-674695. RNA Polymerase II Pre-transcription Events.
R-MMU-75955. RNA Polymerase II Transcription Elongation.

Miscellaneous databases

PROiQ91ZD6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022838.
CleanExiMM_EAF2.
ExpressionAtlasiQ91ZD6. baseline and differential.
GenevisibleiQ91ZD6. MM.

Family and domain databases

InterProiIPR027093. EAF_fam.
IPR019194. Tscrpt_elong_fac_Eaf_N.
[Graphical view]
PANTHERiPTHR15970. PTHR15970. 1 hit.
PfamiPF09816. EAF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEAF2_MOUSE
AccessioniPrimary (citable) accession number: Q91ZD6
Secondary accession number(s): Q7TN80, Q99KD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.