Reviewed, UniProtKB/Swiss-Prot Q91ZB4 (H6ST1_CRIGR)

Names and origin

Protein names

Recommended name:
    Heparan-sulfate 6-O-sulfotransferase 1
      Short name=HS6ST-1
    EC=2.8.2.-

Gene names

Name:	HS6ST1
Synonyms:	6OST1

Organism

Cricetulus griseus (Chinese hamster)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate. Also transfers sulfate to CDSNS-heparin and performs the crucial step modification in the biosynthesis of anticoagulant heparan sulfate (HSact).
Catalytic activity	3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 6-sulfate.
Enzyme regulation	Inhibited by dithiothreitol and stimulated by protamine.
Subcellular location	Membrane; Single-pass type II membrane proteinPotential.
Post-translational modification	N-glycosylated.
Sequence similarities	Belongs to the sulfotransferase 6 family.
Biophysicochemical properties	Kinetic parameters: KM=0.44 µM for PAPS pH dependence: Optimum pH is 6.3.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Heparan-sulfate 6-O-sulfotransferase 1		PRO_0000190800
Regions
	Topological domain	1 – 4	4	Cytoplasmic Potential
	Transmembrane	5 – 27	23	Signal-anchor for type II membrane protein Potential
	Topological domain	28 – 401	374	Lumenal Potential
	Region	86 – 92	7	5'-phosphosulfate-binding Potential
	Region	175 – 183	9	3'-phosphate binding Potential
Amino acid modifications
	Glycosylation	254	1	N-linked (GlcNAc...) Potential
	Glycosylation	310	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	172 – 174	3	TLL → ILV in BAA25761. Ref.2
	Sequence conflict	261 – 263	3	SKR → RAA in BAA25761. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q91ZB4-1 [UniParc]. Last modified June 26, 2007. Version 3. Checksum: 31FDB48A6FC4FB25	FASTA	401	47,080
	10 20 30 40 50 60 MVERASKFVL VVAGSACFML ILYQYAGPGL SLGAPGGRAP PDDLDLFPTP DPHYEKKYYF 70 80 90 100 110 120 PVRELERSLR FDMKGDDVIV FLHIQKTGGT TFGRHLVQNV RLEVPCDCRP GQKKCTCYRP 130 140 150 160 170 180 NRRETWLFSR FSTGWSCGLH ADWTELTNCV PGVLDRRDPA GLRSPRKFYY ITLLRDPVSR 190 200 210 220 230 240 YLSEWRHVQR GATWKTSLHM CDGRTPTPEE LPPCYEGTDW SGCTLQEFMD CPYNLANNRQ 250 260 270 280 290 300 VRMLADLSLV GCYNLSFIPE SKRAQLLLES AKKNLRGMAF FGLTEFQRKT QYLFERTFNL 310 320 330 340 350 360 KFIRPFMQYN STRAGGVEVD EDTIRHIEEL NDLDMQLYDY AKDLFQQRYQ YKRQLERREQ 370 380 390 400 RLRNREERLL HRSKEALPRE DTEEPGRVPT EDYMSHIIEK W

References

[1]	"6-O-sulfotransferase-1 represents a critical enzyme in the anticoagulant heparan sulfate biosynthetic pathway." Zhang L., Beeler D.L., Lawrence R., Lech M., Liu J., Davis J.C., Shriver Z., Sasisekharan R., Rosenberg R.D. J. Biol. Chem. 276:42311-42321(2001) [PubMed: 11551899] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Ovary.
[2]	"Molecular characterization and expression of heparan-sulfate 6-sulfotransferase." Habuchi H., Kobayashi M., Kimata K. J. Biol. Chem. 273:9208-9213(1998) [PubMed: 9535912] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-263, PROTEIN SEQUENCE OF 28-40; 58-65; 95-99; 124-128; 158-163 AND 230-236. Tissue: Ovary.
[3]	"Purification and characterization of heparan sulfate 6-sulfotransferase from the culture medium of Chinese hamster ovary cells." Habuchi H., Habuchi O., Kimata K. J. Biol. Chem. 270:4172-4179(1995) [PubMed: 7876170] [Abstract] Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, GLYCOSYLATION. Tissue: Ovary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY043180 mRNA. Translation: AAL05593.1. AB006180 mRNA. Translation: BAA25761.1.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q91ZB4.
Family and domain databases
InterPro	IPR010635. HS6ST. [Graphical view]
PANTHER	PTHR12812. HS6ST. 1 hit.
Pfam	PF06765. HS6ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

H6ST1_CRIGR

Accession

Primary (citable) accession number: Q91ZB4
Secondary accession number(s): O70158

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	June 26, 2007
Last modified:	November 4, 2008
This is version 28 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)