Q91Z85 (CP17A_PERLE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase
EC=1.14.99.9
EC=4.1.2.30

Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name=Cytochrome P450c17

Gene names

Name:	Cyp17a1
Synonyms:	Cyp17

Organism

Peromyscus leucopus (White-footed mouse)

Taxonomic identifier

10041 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Neotominae › Peromyscus

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty By similarity.
Catalytic activity	A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O. 17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Membrane Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Lyase Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological_process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro steroid 17-alpha-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	Steroid 17-alpha-hydroxylase/17,20 lyase		PRO_0000051939
Sites
	Metal binding	440	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q91Z85 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: E1BAB018B7A6661E	FASTA	509	57,722
	10 20 30 40 50 60 MWELVCLLLL ILAYLFWPKP KTSDAKFPRS LPFLPLVGSL PFLPRHGHMH VNFFKLQEKY 70 80 90 100 110 120 GPIYSLRLGT IPTVIIGQYQ LAREVLIKKG KEFSGRPQMV TLGLLSNQGK GIAFADSDDA 130 140 150 160 170 180 WQLHRKLALS SFALFRDGDQ RLETIICQEA SSLCDLLLTH NEESVDLSQP IFMSVTNIIC 190 200 210 220 230 240 AICFSTSYEN RDPILTTIQT FTEGILNCLD NENLVDIFPW LTIFPNKTLE KIKRHVKVRD 250 260 270 280 290 300 EVLIDMLEKC KEKFNNDSIS NLTDILIQAK MNADNNNTTD HQGSFSDSHI LSTVGDFFGA 310 320 330 340 350 360 GIETTTSVLC WIVAFLLHNP EVKKKIQKEI DQNIGFSRTP TFNDRNHLLM LEATIREVLR 370 380 390 400 410 420 IRPVAPMLIP HKANIDSSIG EFTIPKDTHV VINLWALHHN EKEWDQPDRF MPERFLDATG 430 440 450 460 470 480 SHLITPSLSY LPFGAGPRAC IGEVLARQVL FLYVACLLQR FDLDVPDGAP MPCLKGDPKV 490 500 VFLIDPFKVK VTVRQAWKDA QAEVGTWRP

References

[1]	"Genomic sequence and adrenal expression of Cyp17 during reproductive development in Peromyscus leucopus." Gleason K.K., Hurst F.P., Powers M.A., Riddle S.W., Seashols S., Bradley E.L. Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY054747 Genomic DNA. Translation: AAL12229.1.
3D structure databases
ProteinModelPortal	Q91Z85.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG106944.
Enzyme and pathway databases
UniPathway	UPA00062.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP17A_PERLE

Accession

Primary (citable) accession number: Q91Z85

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program