Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Liprin-alpha-4

Gene

Ppfia4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates (By similarity).By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
Liprin-alpha-4
Alternative name(s):
Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-4
Short name:
PTPRF-interacting protein alpha-4
Gene namesi
Name:Ppfia4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620055. Ppfia4.

Subcellular locationi

  • Cytoplasm By similarity
  • Cell surface By similarity

  • Note: Colocalizes with PTPRF at the cell surface.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – 1043›1043Liprin-alpha-4PRO_0000191033Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei500 – 5001PhosphoserineCombined sources
Modified residuei541 – 5411PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ91Z80.
PRIDEiQ91Z80.

PTM databases

PhosphoSiteiQ91Z80.
SwissPalmiQ91Z80.

Interactioni

Subunit structurei

Forms homodimers and heterodimers with liprins-alpha and liprins-beta. Interacts with the second PTPase domain of PTPRD, PTPRF and PTPRS. Binds RIMS1, RIMS2, GIT1, KIF1A and GRIP1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Gria2P194913EBI-8276907,EBI-77718
Grip1P978797EBI-8276907,EBI-936113
PtprkQ9WUL32EBI-8276907,EBI-8277319

Protein-protein interaction databases

IntActiQ91Z80. 4 interactions.
MINTiMINT-261271.
STRINGi10116.ENSRNOP00000033209.

Structurei

3D structure databases

ProteinModelPortaliQ91Z80.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini688 – 75467SAM 1PROSITE-ProRule annotationAdd
BLAST
Domaini803 – 86765SAM 2PROSITE-ProRule annotationAdd
BLAST
Domaini891 – 96070SAM 3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili24 – 332309Sequence analysisAdd
BLAST
Coiled coili426 – 47045Sequence analysisAdd
BLAST
Coiled coili864 – 89027Sequence analysisAdd
BLAST

Domaini

The N-terminal coiled coil regions mediate homodimerization preferentially and heterodimerization type alpha/alpha. The C-terminal, non-coiled coil regions mediate heterodimerization type alpha/beta and interaction with PTPRD, PTPRF and PTPRS (By similarity).By similarity

Sequence similaritiesi

Belongs to the liprin family. Liprin-alpha subfamily.Curated
Contains 3 SAM (sterile alpha motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0249. Eukaryota.
ENOG410XP8Z. LUCA.
HOGENOMiHOG000017513.
HOVERGENiHBG052330.
InParanoidiQ91Z80.
PhylomeDBiQ91Z80.

Family and domain databases

Gene3Di1.10.150.50. 3 hits.
InterProiIPR029515. Liprin.
IPR030439. PPFIA4.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PANTHERiPTHR12587. PTHR12587. 2 hits.
PTHR12587:SF5. PTHR12587:SF5. 2 hits.
PfamiPF00536. SAM_1. 2 hits.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 3 hits.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 3 hits.
PROSITEiPS50105. SAM_DOMAIN. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Q91Z80-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
DVSSEVEVLK ALKSLFEHHK ALDEKVRERL RAALERVTTL EEQLAGAHQQ
60 70 80 90 100
VSALQQGAGI RDGVAEEEET VDLGPKRLWK DDTGRVEELQ GLLEKQNYEL
110 120 130 140 150
SQARERLVTL SATVTELEED LGTARRDLIK SEELSGKHQR DLREALAQKE
160 170 180 190 200
DMEERITTLE KRYLAAQREA TSIHDLNDKL ENELANKESL HRQCEEKARH
210 220 230 240 250
LQELLEVAEQ KLQQTMRKAE TLPEVEAELS QRIAALTKAE ERHGNIEEHL
260 270 280 290 300
RQLEGQLEEK NQELARVRQR EKMNEDHNKR LSDTVDRLLS ESNERLQLHL
310 320 330 340 350
KERMAALEEK GRLSEEIEKL RQEVDQLKGR GGPFVDGIHS RSHVGSTTDV
360 370 380 390 400
RFSLSTAAHV PPGLHRRYTA LREESAKDWK PAPLPGVLAA TTTPALTVTL
410 420 430 440 450
RSPMWTRMSL GAWWALRLMS SHLVAILDAQ TLAMMLQEQL DAINQEIRMI
460 470 480 490 500
QEEKESTELR AEEIETRVTS GSMEALNLTQ LRKRGSIPTS LTALSLASAS
510 520 530 540 550
PPLSGRSTPK LTSRSAAQDL DRMGVMTLPS DLRKHRRKLL SPVSREENRE
560 570 580 590 600
DKATIKCETS PPSSPRTLRL EKLGHPTLSQ EEGKSALEGQ DSNPSSSNSS
610 620 630 640 650
QDSLHKGAKR KGIKSSIGRL FGKKEKGRLI HLSRDATGHV LLTDSELSLQ
660 670 680 690 700
EPMVPAKLGT QAEKDRRLKK KHQLLEDARR KGMPFAQWDG PTVVSWLELW
710 720 730 740 750
VGMPAWYVAA CRANVKSGAI MSALSDTEIQ REIGISNALH RLKLRLAIQE
760 770 780 790 800
MVSLTSPSAP PTSRTSSGNV WVTHEEMETL ATSTKTDSEE GSWAQTLAYG
810 820 830 840 850
DMNHEWIGNE WLPSLGLPQY RSYFMECLVD ARMLDHLTKK DLRVHLKMVD
860 870 880 890 900
SFHRTSLQYG IMCLKRLNYD RKELEKRREE SQHEIKDVLV WTNDQVVHWV
910 920 930 940 950
QSIGLRDYAG NLHESGVHGA LLALDENFDH NTLALVLQIP TQNTQARQVM
960 970 980 990 1000
EREFNNLLAL GTDRKLDDGE EKVFRRAPSW RKRFRPRDHH SGGMLGTSAE
1010 1020 1030 1040
TLPAGFRVST LGPLQPPPAP PNKIMPEAHS HYLYGHMLSA FRD
Length:1,043
Mass (Da):118,004
Last modified:December 1, 2001 - v1
Checksum:iC7C119B83EDA5C88
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY057064 mRNA. Translation: AAL23695.1.
RefSeqiNP_536334.1. NM_080409.1.
UniGeneiRn.105913.

Genome annotation databases

GeneIDi140592.
KEGGirno:140592.
UCSCiRGD:620055. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY057064 mRNA. Translation: AAL23695.1.
RefSeqiNP_536334.1. NM_080409.1.
UniGeneiRn.105913.

3D structure databases

ProteinModelPortaliQ91Z80.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91Z80. 4 interactions.
MINTiMINT-261271.
STRINGi10116.ENSRNOP00000033209.

PTM databases

PhosphoSiteiQ91Z80.
SwissPalmiQ91Z80.

Proteomic databases

PaxDbiQ91Z80.
PRIDEiQ91Z80.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi140592.
KEGGirno:140592.
UCSCiRGD:620055. rat.

Organism-specific databases

CTDi8497.
RGDi620055. Ppfia4.

Phylogenomic databases

eggNOGiKOG0249. Eukaryota.
ENOG410XP8Z. LUCA.
HOGENOMiHOG000017513.
HOVERGENiHBG052330.
InParanoidiQ91Z80.
PhylomeDBiQ91Z80.

Miscellaneous databases

NextBioi620560.

Family and domain databases

Gene3Di1.10.150.50. 3 hits.
InterProiIPR029515. Liprin.
IPR030439. PPFIA4.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PANTHERiPTHR12587. PTHR12587. 2 hits.
PTHR12587:SF5. PTHR12587:SF5. 2 hits.
PfamiPF00536. SAM_1. 2 hits.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 3 hits.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 3 hits.
PROSITEiPS50105. SAM_DOMAIN. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mukherjee K., Wang Y., Suedhof T.C.
    Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "RIM1alpha forms a protein scaffold for regulating neurotransmitter release at the active zone."
    Schoch S., Castillo P.E., Jo T., Mukherjee K., Geppert M., Wang Y., Schmitz F., Malenka R.C., Suedhof T.C.
    Nature 415:321-326(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RIMS1 AND RIMS2.
    Tissue: Brain.
  3. "Interaction between GRIP and liprin-alpha/SYD2 is required for AMPA receptor targeting."
    Wyszynski M., Kim E., Dunah A.W., Passafaro M., Valtschanoff J.G., Serra-Pages C., Streuli M., Weinberg R.J., Sheng M.
    Neuron 34:39-52(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRIP1.
  4. "Interaction between liprin-alpha and GIT1 is required for AMPA receptor targeting."
    Ko J., Kim S., Valtschanoff J.G., Shin H., Lee J.R., Sheng M., Premont R.T., Weinberg R.J., Kim E.
    J. Neurosci. 23:1667-1677(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GIT1.
  5. "Association of the kinesin motor KIF1A with the multimodular protein liprin-alpha."
    Shin H., Wyszynski M., Huh K.H., Valtschanoff J.G., Lee J.R., Ko J., Streuli M., Weinberg R.J., Sheng M., Kim E.
    J. Biol. Chem. 278:11393-11401(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KIF1A.
  6. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-500 AND SER-541, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiLIPA4_RAT
AccessioniPrimary (citable) accession number: Q91Z80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: December 1, 2001
Last modified: February 17, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.