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Protein

Glyoxylate reductase/hydroxypyruvate reductase

Gene

Grhpr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme with hydroxy-pyruvate reductase, glyoxylate reductase and D-glycerate dehydrogenase enzymatic activities. Reduces hydroxypyruvate to D-glycerate, glyoxylate to glycolate oxidizes D-glycerate to hydroxypyruvate (By similarity).By similarity

Catalytic activityi

Glycolate + NADP+ = glyoxylate + NADPH.
D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei217 – 2171NADPBy similarity
Binding sitei243 – 2431NADP; via carbonyl oxygenBy similarity
Binding sitei245 – 2451SubstrateBy similarity
Binding sitei269 – 2691SubstrateBy similarity
Sitei274 – 2741Raises pKa of active site HisBy similarity
Active sitei293 – 2931Proton donorBy similarity
Binding sitei295 – 2951NADP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi162 – 1643NADPBy similarity
Nucleotide bindingi185 – 1884NADPBy similarity

GO - Molecular functioni

  1. carboxylic acid binding Source: Ensembl
  2. glycerate dehydrogenase activity Source: MGI
  3. glyoxylate reductase (NADP) activity Source: MGI
  4. hydroxypyruvate reductase activity Source: MGI
  5. NAD binding Source: InterPro
  6. NADPH binding Source: MGI
  7. protein homodimerization activity Source: MGI

GO - Biological processi

  1. dicarboxylic acid metabolic process Source: Ensembl
  2. excretion Source: MGI
  3. oxidation-reduction process Source: MGI
  4. protein oligomerization Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_291032. Glyoxylate metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyoxylate reductase/hydroxypyruvate reductase (EC:1.1.1.79, EC:1.1.1.81)
Gene namesi
Name:Grhpr
Synonyms:Glxr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1923488. Grhpr.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytosol Source: Ensembl
  3. extracellular vesicular exosome Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Glyoxylate reductase/hydroxypyruvate reductasePRO_0000075945Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361PhosphoserineBy similarity
Modified residuei298 – 2981PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ91Z53.
PaxDbiQ91Z53.
PRIDEiQ91Z53.

2D gel databases

REPRODUCTION-2DPAGEQ91Z53.

PTM databases

PhosphoSiteiQ91Z53.

Expressioni

Gene expression databases

BgeeiQ91Z53.
CleanExiMM_GRHPR.
ExpressionAtlasiQ91Z53. baseline and differential.
GenevestigatoriQ91Z53.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ91Z53. 4 interactions.
MINTiMINT-1868690.

Structurei

3D structure databases

ProteinModelPortaliQ91Z53.
SMRiQ91Z53. Positions 5-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni83 – 842Substrate bindingBy similarity
Regioni293 – 2964Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1052.
GeneTreeiENSGT00510000046913.
HOGENOMiHOG000136700.
HOVERGENiHBG051838.
InParanoidiQ91Z53.
KOiK00049.
OMAiKSIGPDW.
OrthoDBiEOG7K3TMJ.
PhylomeDBiQ91Z53.
TreeFamiTF324791.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
PROSITEiPS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91Z53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPARLMKVF VTGPLPAEGR AALAQAADCE VEQWNSDDPI PRKDLEQGVV
60 70 80 90 100
GAHGLLCRLS DRVDKKLLDA AGANLRVIST LSVGVDHLAL DEIKKRGIRV
110 120 130 140 150
GYTPGVLTDA TAELAVSLLL TTCRRLPEAI EEVKNGGWSS WSPLWMCGYG
160 170 180 190 200
LSQSTVGIVG LGRIGQAIAR RLKPFGVQRF LYTGRQPRPQ EAAEFQAEFV
210 220 230 240 250
PIAQLAAESD FIVVSCSLTP DTMGLCSKDF FQKMKNTAIF INISRGDVVN
260 270 280 290 300
QEDLYQALAS GQIAAAGLDV TTPEPLPPSH PLLTLKNCVI LPHIGSATYK
310 320
TRNTMSLLAA NNLLAGLRGE AMPSELKL
Length:328
Mass (Da):35,329
Last modified:December 1, 2001 - v1
Checksum:iBDEC1ADEC1E18153
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY113690 mRNA. Translation: AAM52985.1.
BC010194 mRNA. Translation: AAH10194.1.
CCDSiCCDS18128.1.
RefSeqiNP_525028.1. NM_080289.1.
UniGeneiMm.196574.

Genome annotation databases

EnsembliENSMUST00000045078; ENSMUSP00000047218; ENSMUSG00000035637.
GeneIDi76238.
KEGGimmu:76238.
UCSCiuc008ssb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY113690 mRNA. Translation: AAM52985.1.
BC010194 mRNA. Translation: AAH10194.1.
CCDSiCCDS18128.1.
RefSeqiNP_525028.1. NM_080289.1.
UniGeneiMm.196574.

3D structure databases

ProteinModelPortaliQ91Z53.
SMRiQ91Z53. Positions 5-328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91Z53. 4 interactions.
MINTiMINT-1868690.

PTM databases

PhosphoSiteiQ91Z53.

2D gel databases

REPRODUCTION-2DPAGEQ91Z53.

Proteomic databases

MaxQBiQ91Z53.
PaxDbiQ91Z53.
PRIDEiQ91Z53.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045078; ENSMUSP00000047218; ENSMUSG00000035637.
GeneIDi76238.
KEGGimmu:76238.
UCSCiuc008ssb.1. mouse.

Organism-specific databases

CTDi9380.
MGIiMGI:1923488. Grhpr.

Phylogenomic databases

eggNOGiCOG1052.
GeneTreeiENSGT00510000046913.
HOGENOMiHOG000136700.
HOVERGENiHBG051838.
InParanoidiQ91Z53.
KOiK00049.
OMAiKSIGPDW.
OrthoDBiEOG7K3TMJ.
PhylomeDBiQ91Z53.
TreeFamiTF324791.

Enzyme and pathway databases

ReactomeiREACT_291032. Glyoxylate metabolism.

Miscellaneous databases

NextBioi344835.
PROiQ91Z53.
SOURCEiSearch...

Gene expression databases

BgeeiQ91Z53.
CleanExiMM_GRHPR.
ExpressionAtlasiQ91Z53. baseline and differential.
GenevestigatoriQ91Z53.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
PROSITEiPS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the mouse GRHPR cDNA from liver."
    Cramer S.D.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129/SvEv.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.
  3. Lubec G., Klug S.
    Submitted (MAR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 44-58 AND 303-318, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Hippocampus.

Entry informationi

Entry nameiGRHPR_MOUSE
AccessioniPrimary (citable) accession number: Q91Z53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: December 1, 2001
Last modified: April 1, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.