Q91Z53 (GRHPR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyoxylate reductase/hydroxypyruvate reductase
EC=1.1.1.79
EC=1.1.1.81

Gene names

Name:	Grhpr
Synonyms:	Glxr

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Enzyme with hydroxy-pyruvate reductase, glyoxylate reductase and D-glycerate dehydrogenase enzymatic activities. Reduces hydroxypyruvate to D-glycerate, glyoxylate to glycolate oxidizes D-glycerate to hydroxypyruvate By similarity.
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	excretion Inferred from electronic annotation. Source: Compara protein oligomerization Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro NADPH binding Inferred from electronic annotation. Source: Compara glycerate dehydrogenase activity Inferred from electronic annotation. Source: Compara glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: EC hydroxypyruvate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Glyoxylate reductase/hydroxypyruvate reductase		PRO_0000075945
Regions
	Nucleotide binding	162 – 164	3	NADP By similarity
	Nucleotide binding	185 – 188	4	NADP By similarity
	Region	83 – 84	2	Substrate binding By similarity
	Region	293 – 296	4	Substrate binding By similarity
Sites
	Active site	293	1	Proton donor By similarity
	Binding site	217	1	NADP By similarity
	Binding site	243	1	NADP; via carbonyl oxygen By similarity
	Binding site	245	1	Substrate By similarity
	Binding site	269	1	Substrate By similarity
	Binding site	295	1	NADP; via amide nitrogen By similarity
	Site	274	1	Raises pKa of active site His By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q91Z53 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: BDEC1ADEC1E18153	FASTA	328	35,329
	10 20 30 40 50 60 MKPARLMKVF VTGPLPAEGR AALAQAADCE VEQWNSDDPI PRKDLEQGVV GAHGLLCRLS 70 80 90 100 110 120 DRVDKKLLDA AGANLRVIST LSVGVDHLAL DEIKKRGIRV GYTPGVLTDA TAELAVSLLL 130 140 150 160 170 180 TTCRRLPEAI EEVKNGGWSS WSPLWMCGYG LSQSTVGIVG LGRIGQAIAR RLKPFGVQRF 190 200 210 220 230 240 LYTGRQPRPQ EAAEFQAEFV PIAQLAAESD FIVVSCSLTP DTMGLCSKDF FQKMKNTAIF 250 260 270 280 290 300 INISRGDVVN QEDLYQALAS GQIAAAGLDV TTPEPLPPSH PLLTLKNCVI LPHIGSATYK 310 320 TRNTMSLLAA NNLLAGLRGE AMPSELKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the mouse GRHPR cDNA from liver." Cramer S.D. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129/SvEv. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary tumor.
[3]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 44-58 AND 303-318, MASS SPECTROMETRY. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY113690 mRNA. Translation: AAM52985.1. BC010194 mRNA. Translation: AAH10194.1.
IPI	IPI00130530.
RefSeq	NP_525028.1. NM_080289.1.
UniGene	Mm.196574.
3D structure databases
ProteinModelPortal	Q91Z53.
SMR	Q91Z53. Positions 5-328.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1868690.
PTM databases
PhosphoSite	Q91Z53.
2D gel databases
REPRODUCTION-2DPAGE	Q91Z53.
Proteomic databases
PaxDb	Q91Z53.
PRIDE	Q91Z53.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000045078; ENSMUSP00000047218; ENSMUSG00000035637.
GeneID	76238.
KEGG	mmu:76238.
UCSC	uc008ssb.1. mouse.
Organism-specific databases
CTD	9380.
MGI	MGI:1923488. Grhpr.
Phylogenomic databases
eggNOG	COG1052.
GeneTree	ENSGT00510000046913.
HOGENOM	HOG000136700.
HOVERGEN	HBG051838.
InParanoid	Q91Z53.
KO	K00049.
OMA	KTRNTMS.
OrthoDB	EOG4GHZPX.
Gene expression databases
ArrayExpress	Q91Z53.
Bgee	Q91Z53.
CleanEx	MM_GRHPR.
Genevestigator	Q91Z53.
GermOnline	ENSMUSG00000035637. Mus musculus.
Family and domain databases
Gene3D	3.40.50.720. 2 hits.
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	344835.
SOURCE	Search...

Entry information

Entry name

GRHPR_MOUSE

Accession

Primary (citable) accession number: Q91Z53

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	December 1, 2001
Last modified:	May 29, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program