Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein
Submitted name:

Flap endonuclease 1

Gene

Fen1

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.SAAS annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

EndonucleaseImported, Hydrolase, Nuclease

Enzyme and pathway databases

ReactomeiR-MMU-110362. POLB-Dependent Long Patch Base Excision Repair.
R-MMU-174437. Removal of the Flap Intermediate from the C-strand.
R-MMU-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-MMU-5685939. HDR through MMEJ (alt-NHEJ).
R-MMU-69166. Removal of the Flap Intermediate.

Names & Taxonomyi

Protein namesi
Submitted name:
Flap endonuclease 1Imported
Submitted name:
Flap structure specific endonuclease 1Imported
Submitted name:
Flap structure specific endonuclease 1, isoform CRA_cImported
Gene namesi
Name:Fen1Imported
ORF Names:mCG_1942Imported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:102779. Fen1.

Subcellular locationi

  • Mitochondrion SAAS annotation
  • Nucleusnucleoplasm SAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

MitochondrionSAAS annotation, NucleusSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025651.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 107107XPGNInterPro annotationAdd
BLAST
Domaini146 – 21873XPGIInterPro annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili97 – 12428Sequence analysisAdd
BLAST

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiKOG2519. Eukaryota.
COG0258. LUCA.
GeneTreeiENSGT00640000091478.
HOVERGENiHBG000844.
KOiK04799.
OMAiGSQDYDS.
TreeFamiTF105701.

Family and domain databases

Gene3Di3.40.50.1010. 1 hit.
HAMAPiMF_00614. Fen.
InterProiIPR020045. 5-3_exonuclease_C.
IPR023426. Flap_endonuc.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081. PTHR11081. 1 hit.
PfamiPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSiPR00853. XPGRADSUPER.
SMARTiSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 1 hit.
PROSITEiPS00841. XPG_1. 1 hit.
PS00842. XPG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91Z50-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIHGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG
60 70 80 90 100
DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR
110 120 130 140 150
SERRAEAEKQ LQQAQEAGME EEVEKFTKRL VKVTKQHNDE CKHLLSLMGI
160 170 180 190 200
PYLDAPSEAE ASCAALAKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK
210 220 230 240 250
KLPIQEFHLS RVLQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI
260 270 280 290 300
QKHKSIEEIV RRLDPSKYPV PENWLHKEAQ QLFLEPEVLD PESVELKWSE
310 320 330 340 350
PNEEELVKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL
360 370 380
SSAKRKEPEP KGPAKKKAKT GGAGKFRRGK
Length:380
Mass (Da):42,623
Last modified:December 1, 2001 - v1
Checksum:i1BE903288B46520D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC132148 Genomic DNA. No translation available.
BC010203 mRNA. Translation: AAH10203.1.
AK046256 mRNA. Translation: BAC32660.1.
AK078778 mRNA. Translation: BAC37390.1.
AB191468 Genomic DNA. Translation: BAD52443.1.
AK153889 mRNA. Translation: BAE32236.1.
AK161047 mRNA. Translation: BAE36169.1.
AK166948 mRNA. Translation: BAE39135.1.
CH466534 Genomic DNA. Translation: EDL41342.1.
RefSeqiNP_001258543.1. NM_001271614.1.
NP_001258544.1. NM_001271615.1.
NP_032025.2. NM_007999.4.
XP_006526724.1. XM_006526661.2.
XP_006526725.1. XM_006526662.2.
XP_006526726.1. XM_006526663.2.
XP_006526727.1. XM_006526664.2.
UniGeneiMm.2952.

Genome annotation databases

EnsembliENSMUST00000025651; ENSMUSP00000025651; ENSMUSG00000024742.
ENSMUST00000116542; ENSMUSP00000112241; ENSMUSG00000024742.
ENSMUST00000142241; ENSMUSP00000119221; ENSMUSG00000024742.
ENSMUST00000156291; ENSMUSP00000117246; ENSMUSG00000024742.
GeneIDi14156.
KEGGimmu:14156.
UCSCiuc008gpf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC132148 Genomic DNA. No translation available.
BC010203 mRNA. Translation: AAH10203.1.
AK046256 mRNA. Translation: BAC32660.1.
AK078778 mRNA. Translation: BAC37390.1.
AB191468 Genomic DNA. Translation: BAD52443.1.
AK153889 mRNA. Translation: BAE32236.1.
AK161047 mRNA. Translation: BAE36169.1.
AK166948 mRNA. Translation: BAE39135.1.
CH466534 Genomic DNA. Translation: EDL41342.1.
RefSeqiNP_001258543.1. NM_001271614.1.
NP_001258544.1. NM_001271615.1.
NP_032025.2. NM_007999.4.
XP_006526724.1. XM_006526661.2.
XP_006526725.1. XM_006526662.2.
XP_006526726.1. XM_006526663.2.
XP_006526727.1. XM_006526664.2.
UniGeneiMm.2952.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025651.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025651; ENSMUSP00000025651; ENSMUSG00000024742.
ENSMUST00000116542; ENSMUSP00000112241; ENSMUSG00000024742.
ENSMUST00000142241; ENSMUSP00000119221; ENSMUSG00000024742.
ENSMUST00000156291; ENSMUSP00000117246; ENSMUSG00000024742.
GeneIDi14156.
KEGGimmu:14156.
UCSCiuc008gpf.2. mouse.

Organism-specific databases

CTDi2237.
MGIiMGI:102779. Fen1.

Phylogenomic databases

eggNOGiKOG2519. Eukaryota.
COG0258. LUCA.
GeneTreeiENSGT00640000091478.
HOVERGENiHBG000844.
KOiK04799.
OMAiGSQDYDS.
TreeFamiTF105701.

Enzyme and pathway databases

ReactomeiR-MMU-110362. POLB-Dependent Long Patch Base Excision Repair.
R-MMU-174437. Removal of the Flap Intermediate from the C-strand.
R-MMU-5651801. PCNA-Dependent Long Patch Base Excision Repair.
R-MMU-5685939. HDR through MMEJ (alt-NHEJ).
R-MMU-69166. Removal of the Flap Intermediate.

Miscellaneous databases

ChiTaRSiFen1. mouse.
NextBioi285276.
SOURCEiSearch...

Family and domain databases

Gene3Di3.40.50.1010. 1 hit.
HAMAPiMF_00614. Fen.
InterProiIPR020045. 5-3_exonuclease_C.
IPR023426. Flap_endonuc.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081. PTHR11081. 1 hit.
PfamiPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSiPR00853. XPGRADSUPER.
SMARTiSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 1 hit.
PROSITEiPS00841. XPG_1. 1 hit.
PS00842. XPG_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "High-efficiency full-length cDNA cloning."
    Carninci P., Hayashizaki Y.
    Methods Enzymol. 303:19-44(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported and NODImported.
    Tissue: Corpora quadrigeminaImported, SkinImported, TestisImported and ThymusImported.
  2. "Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes."
    Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M., Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.
    Genome Res. 10:1617-1630(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported and NODImported.
    Tissue: Corpora quadrigeminaImported, SkinImported, TestisImported and ThymusImported.
  3. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported and NODImported.
    Tissue: Corpora quadrigeminaImported, SkinImported, TestisImported and ThymusImported.
  4. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported and NODImported.
    Tissue: Corpora quadrigeminaImported, SkinImported, TestisImported and ThymusImported.
  5. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: Corpora quadrigeminaImported.
  6. "Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs."
    The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team
    Nature 420:563-573(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported and NODImported.
    Tissue: Corpora quadrigeminaImported, SkinImported, TestisImported and ThymusImported.
  7. "A comparison of whole-genome shotgun-derived mouse chromosome 16 and the human genome."
    Mural R.J., Adams M.D., Myers E.W., Smith H.O., Miklos G.L., Wides R., Halpern A., Li P.W., Sutton G.G., Nadeau J., Salzberg S.L., Holt R.A., Kodira C.D., Lu F., Chen L., Deng Z., Evangelista C.C., Gan W.
    , Heiman T.J., Li J., Li Z., Merkulov G.V., Milshina N.V., Naik A.K., Qi R., Shue B.C., Wang A., Wang J., Wang X., Yan X., Ye J., Yooseph S., Zhao Q., Zheng L., Zhu S.C., Biddick K., Bolanos R., Delcher A.L., Dew I.M., Fasulo D., Flanigan M.J., Huson D.H., Kravitz S.A., Miller J.R., Mobarry C.M., Reinert K., Remington K.A., Zhang Q., Zheng X.H., Nusskern D.R., Lai Z., Lei Y., Zhong W., Yao A., Guan P., Ji R.R., Gu Z., Wang Z.Y., Zhong F., Xiao C., Chiang C.C., Yandell M., Wortman J.R., Amanatides P.G., Hladun S.L., Pratts E.C., Johnson J.E., Dodson K.L., Woodford K.J., Evans C.A., Gropman B., Rusch D.B., Venter E., Wang M., Smith T.J., Houck J.T., Tompkins D.E., Haynes C., Jacob D., Chin S.H., Allen D.R., Dahlke C.E., Sanders R., Li K., Liu X., Levitsky A.A., Majoros W.H., Chen Q., Xia A.C., Lopez J.R., Donnelly M.T., Newman M.H., Glodek A., Kraft C.L., Nodell M., Ali F., An H.J., Baldwin-Pitts D., Beeson K.Y., Cai S., Carnes M., Carver A., Caulk P.M., Center A., Chen Y.H., Cheng M.L., Coyne M.D., Crowder M., Danaher S., Davenport L.B., Desilets R., Dietz S.M., Doup L., Dullaghan P., Ferriera S., Fosler C.R., Gire H.C., Gluecksmann A., Gocayne J.D., Gray J., Hart B., Haynes J., Hoover J., Howland T., Ibegwam C., Jalali M., Johns D., Kline L., Ma D.S., MacCawley S., Magoon A., Mann F., May D., McIntosh T.C., Mehta S., Moy L., Moy M.C., Murphy B.J., Murphy S.D., Nelson K.A., Nuri Z., Parker K.A., Prudhomme A.C., Puri V.N., Qureshi H., Raley J.C., Reardon M.S., Regier M.A., Rogers Y.H., Romblad D.L., Schutz J., Scott J.L., Scott R., Sitter C.D., Smallwood M., Sprague A.C., Stewart E., Strong R.V., Suh E., Sylvester K., Thomas R., Tint N.N., Tsonis C., Wang G., Wang G., Williams M.S., Williams S.M., Windsor S.M., Wolfe K., Wu M.M., Zaveri J., Chaturvedi K., Gabrielian A.E., Ke Z., Sun J., Subramanian G., Venter J.C., Pfannkoch C.M., Barnstead M., Stephenson L.D.
    Science 296:1661-1671(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MixedImported.
  8. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: TestisImported.
  9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Gerhard D.S., Wagner L., Feingold E.A., Shenmen C.M., Grouse L.H., Schuler G., Klein S.L., Old S., Rasooly R., Good P., Guyer M., Peck A.M., Derge J.G., Lipman D., Collins F.S., Jang W., Sherry S., Feolo M.
    , Misquitta L., Lee E., Rotmistrovsky K., Greenhut S.F., Schaefer C.F., Buetow K., Bonner T.I., Haussler D., Kent J., Kiekhaus M., Furey T., Brent M., Prange C., Schreiber K., Shapiro N., Bhat N.K., Hopkins R.F., Hsie F., Driscoll T., Soares M.B., Casavant T.L., Scheetz T.E., Brown-stein M.J., Usdin T.B., Toshiyuki S., Carninci P., Piao Y., Dudekula D.B., Ko M.S., Kawakami K., Suzuki Y., Sugano S., Gruber C.E., Smith M.R., Simmons B., Moore T., Waterman R., Johnson S.L., Ruan Y., Wei C.L., Mathavan S., Gunaratne P.H., Wu J., Garcia A.M., Hulyk S.W., Fuh E., Yuan Y., Sneed A., Kowis C., Hodgson A., Muzny D.M., McPherson J., Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Madari A., Young A.C., Wetherby K.D., Granite S.J., Kwong P.N., Brinkley C.P., Pearson R.L., Bouffard G.G., Blakesly R.W., Green E.D., Dickson M.C., Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M., Butterfield Y.S., Griffith M., Griffith O.L., Krzywinski M.I., Liao N., Morin R., Morrin R., Palmquist D., Petrescu A.S., Skalska U., Smailus D.E., Stott J.M., Schnerch A., Schein J.E., Jones S.J., Holt R.A., Baross A., Marra M.A., Clifton S., Makowski K.A., Bosak S., Malek J.
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/NImported.
    Tissue: Mammary tumor. C3Imported.
  10. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: NODImported.
    Tissue: ThymusImported.
  11. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: SkinImported.
  12. "Structure and transcription promoter activity of mouse flap endonuclease 1 gene: alternative splicing and bidirectional promoter."
    Emoto M., Miki M., Sarker A.H., Nakamura T., Seki Y., Seki S., Ikeda S.
    Gene 357:47-54(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: LeukocyteImported.
  13. "The Transcriptional Landscape of the Mammalian Genome."
    The FANTOM Consortium, Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group)
    Science 309:1559-1563(2005)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported and NODImported.
    Tissue: Corpora quadrigeminaImported, SkinImported, TestisImported and ThymusImported.
  14. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported and NODImported.
    Tissue: Corpora quadrigeminaImported, SkinImported, TestisImported and ThymusImported.
  15. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: MixedImported.
  16. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6JImported.
  17. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  18. Ensembl
    Submitted (FEB-2012) to UniProtKB
    Cited for: IDENTIFICATION.
    Strain: C57BL/6JImported.
  19. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiQ91Z50_MOUSE
AccessioniPrimary (citable) accession number: Q91Z50
Entry historyi
Integrated into UniProtKB/TrEMBL: December 1, 2001
Last sequence update: December 1, 2001
Last modified: May 11, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.