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Protein

Polypyrimidine tract-binding protein 2

Gene

Ptbp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. In addition to its function in pre-mRNA splicing, plays also a role in the regulation of translation.2 Publications

GO - Molecular functioni

  • mRNA binding Source: MGI
  • pre-mRNA binding Source: Ensembl

GO - Biological processi

  • cerebellum development Source: Ensembl
  • mRNA splice site selection Source: MGI
  • negative regulation of RNA splicing Source: MGI
  • regulation of neural precursor cell proliferation Source: MGI
  • spinal cord development Source: Ensembl

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Names & Taxonomyi

Protein namesi
Recommended name:
Polypyrimidine tract-binding protein 2
Alternative name(s):
Brain-enriched polypyrimidine tract-binding protein
Short name:
Brain-enriched PTB
Neural polypyrimidine tract-binding protein
RRM-type RNA-binding protein brPTB
Gene namesi
Name:Ptbp2
Synonyms:Brptb, Nptb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1860489 Ptbp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002329291 – 531Polypyrimidine tract-binding protein 2Add BLAST531

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei26PhosphoserineCombined sources1
Modified residuei27PhosphoserineCombined sources1
Modified residuei308PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ91Z31
PeptideAtlasiQ91Z31
PRIDEiQ91Z31

PTM databases

iPTMnetiQ91Z31
PhosphoSitePlusiQ91Z31

Expressioni

Tissue specificityi

Mainly expressed in brain, including cerebellum, brainstem, spinal cord, and hypothalamus. Also expressed in the peripheral nervous system and neural crest derivatives, including the dorsal root and trigeminal ganglia, the cochlear spiral and intestinal ganglion cells, and the adrenal medulla. Also detected to a lower extent in testis, heart, liver, lung, skeletal muscle and thymus (at protein level).2 Publications

Developmental stagei

Expressed in whole brain tissues from E11.5 to adulthood.2 Publications

Gene expression databases

BgeeiENSMUSG00000028134
CleanExiMM_PTBP2
ExpressionAtlasiQ91Z31 baseline and differential
GenevisibleiQ91Z31 MM

Interactioni

Subunit structurei

Monomer. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Part of a ternary complex containing KHSRP and HNRPH1 (By similarity). Interacts with NOVA1; the interaction is direct. Interacts with NOVA2; the interaction is direct.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AicdaQ9WVE04EBI-647632,EBI-3835567

Protein-protein interaction databases

BioGridi207834, 3 interactors
CORUMiQ91Z31
IntActiQ91Z31, 3 interactors
MINTiQ91Z31
STRINGi10090.ENSMUSP00000029780

Structurei

3D structure databases

ProteinModelPortaliQ91Z31
SMRiQ91Z31
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini59 – 133RRM 1PROSITE-ProRule annotationAdd BLAST75
Domaini181 – 257RRM 2PROSITE-ProRule annotationAdd BLAST77
Domaini338 – 412RRM 3PROSITE-ProRule annotationAdd BLAST75
Domaini455 – 529RRM 4PROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi315 – 324Poly-Ala10

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1190 Eukaryota
ENOG410XPMZ LUCA
GeneTreeiENSGT00550000074508
HOGENOMiHOG000231699
HOVERGENiHBG069548
InParanoidiQ91Z31
KOiK14948
OMAiAQQAKLX
OrthoDBiEOG091G0Z5R
PhylomeDBiQ91Z31
TreeFamiTF319824

Family and domain databases

CDDicd12778 RRM1_PTBP2, 1 hit
cd12693 RRM2_PTBP1_like, 1 hit
cd12696 RRM3_PTBP2, 1 hit
cd12702 RRM4_PTBP2, 1 hit
Gene3Di3.30.70.330, 4 hits
InterProiView protein in InterPro
IPR006536 HnRNP-L/PTB
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR034798 PTBP1/2/3_RRM2
IPR035002 PTBP2_RRM1
IPR034799 PTBP2_RRM3
IPR034800 PTBP2_RRM4
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PfamiView protein in Pfam
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00360 RRM, 4 hits
SUPFAMiSSF54928 SSF54928, 4 hits
TIGRFAMsiTIGR01649 hnRNP-L_PTB, 1 hit
PROSITEiView protein in PROSITE
PS50102 RRM, 4 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91Z31-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGIVTEVAV GVKRGSDELL SGSVLSSPNS NMSGMVVTAN GNDSKKFKGE
60 70 80 90 100
DKMDGAPSRV LHIRKLPGEV TETEVIALGL PFGKVTNILM LKGKNQAFLE
110 120 130 140 150
LATEEAAITM VNYYSAVTPH LRNQPIYIQY SNHKELKTDN TLNQRAQVVL
160 170 180 190 200
QAVTAVQTAN TPLSGTTVSE SAVTPAQSPV LRIIIDNMYY PVTLDVLHQI
210 220 230 240 250
FSKFGAVLKI ITFTKNNQFQ ALLQYGDPVN AQQAKLALDG QNIYNACCTL
260 270 280 290 300
RIDFSKLVNL NVKYNNDKSR DYTRPDLPSG DGQPALDPAI AAAFAKETSL
310 320 330 340 350
LAVPGALSPL AIPNAAAAAA AAAAGRVGMP GVSAGGNTVL LVSNLNEEMV
360 370 380 390 400
TPQSLFTLFG VYGDVQRVKI LYNKKDSALI QMADGNQSQL AMNHLNGQKM
410 420 430 440 450
YGKIIRVTLS KHQTVQLPRE GLDDQGLTKD FGNSPLHRFK KPGSKNFQNI
460 470 480 490 500
FPPSATLHLS NIPPSVAEED LRTLFANTGG TVKAFKFFQD HKMALLQMAT
510 520 530
VEEAIQALID LHNYNLGENH HLRVSFSKST I
Length:531
Mass (Da):57,489
Last modified:April 18, 2006 - v2
Checksum:i3A93CA4E3E1D6AA3
GO
Isoform 2 (identifier: Q91Z31-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     489-489: Q → QR

Show »
Length:532
Mass (Da):57,645
Checksum:i732A3CC8C6D47512
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93G → E in AAH10255 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018019489Q → QR in isoform 2. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095718 mRNA Translation: AAF21807.2
AK013734 mRNA Translation: BAB28977.1
AK137348 mRNA Translation: BAE23317.1
BC010255 mRNA Translation: AAH10255.1
AY333752 Genomic DNA Translation: AAQ01150.1
BK000527 mRNA Translation: DAA00061.1
CCDSiCCDS38616.1 [Q91Z31-2]
CCDS80010.1 [Q91Z31-1]
RefSeqiNP_001297640.1, NM_001310711.1 [Q91Z31-1]
NP_062423.1, NM_019550.2 [Q91Z31-2]
UniGeneiMm.29966

Genome annotation databases

EnsembliENSMUST00000029780; ENSMUSP00000029780; ENSMUSG00000028134 [Q91Z31-2]
ENSMUST00000200097; ENSMUSP00000143510; ENSMUSG00000028134 [Q91Z31-1]
GeneIDi56195
KEGGimmu:56195
UCSCiuc008rdi.1 mouse [Q91Z31-1]
uc008rdk.2 mouse [Q91Z31-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPTBP2_MOUSE
AccessioniPrimary (citable) accession number: Q91Z31
Secondary accession number(s): Q7TNW7
, Q9CUW2, Q9QYC2, Q9R0V9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: April 18, 2006
Last modified: March 28, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health