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Protein

Polypyrimidine tract-binding protein 2

Gene

Ptbp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. May antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. In addition to its function in pre-mRNA splicing, plays also a role in the regulation of translation.2 Publications

GO - Molecular functioni

  • mRNA binding Source: MGI
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: MGI
  • pre-mRNA binding Source: Ensembl

GO - Biological processi

  • cerebellum development Source: Ensembl
  • mRNA splice site selection Source: MGI
  • negative regulation of RNA splicing Source: MGI
  • regulation of neural precursor cell proliferation Source: MGI
  • spinal cord development Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polypyrimidine tract-binding protein 2
Alternative name(s):
Brain-enriched polypyrimidine tract-binding protein
Short name:
Brain-enriched PTB
Neural polypyrimidine tract-binding protein
RRM-type RNA-binding protein brPTB
Gene namesi
Name:Ptbp2
Synonyms:Brptb, Nptb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1860489. Ptbp2.

Subcellular locationi

GO - Cellular componenti

  • growth cone Source: Ensembl
  • neuronal cell body Source: Ensembl
  • spliceosomal complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 531531Polypyrimidine tract-binding protein 2PRO_0000232929Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei26 – 261PhosphoserineCombined sources
Modified residuei27 – 271PhosphoserineCombined sources
Modified residuei308 – 3081PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ91Z31.
PaxDbiQ91Z31.
PeptideAtlasiQ91Z31.
PRIDEiQ91Z31.

PTM databases

iPTMnetiQ91Z31.
PhosphoSiteiQ91Z31.

Expressioni

Tissue specificityi

Mainly expressed in brain, including cerebellum, brainstem, spinal cord, and hypothalamus. Also expressed in the peripheral nervous system and neural crest derivatives, including the dorsal root and trigeminal ganglia, the cochlear spiral and intestinal ganglion cells, and the adrenal medulla. Also detected to a lower extent in testis, heart, liver, lung, skeletal muscle and thymus (at protein level).2 Publications

Developmental stagei

Expressed in whole brain tissues from E11.5 to adulthood.2 Publications

Gene expression databases

BgeeiENSMUSG00000028134.
CleanExiMM_PTBP2.
ExpressionAtlasiQ91Z31. baseline and differential.
GenevisibleiQ91Z31. MM.

Interactioni

Subunit structurei

Monomer. Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Part of a ternary complex containing KHSRP and HNRPH1 (By similarity). Interacts with NOVA1; the interaction is direct. Interacts with NOVA2; the interaction is direct.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AicdaQ9WVE04EBI-647632,EBI-3835567

Protein-protein interaction databases

BioGridi207834. 3 interactions.
IntActiQ91Z31. 3 interactions.
MINTiMINT-4130609.
STRINGi10090.ENSMUSP00000029780.

Structurei

3D structure databases

ProteinModelPortaliQ91Z31.
SMRiQ91Z31. Positions 51-138, 177-281, 325-531.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini59 – 13375RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini181 – 25777RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini338 – 41275RRM 3PROSITE-ProRule annotationAdd
BLAST
Domaini455 – 52975RRM 4PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi315 – 32410Poly-Ala

Sequence similaritiesi

Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1190. Eukaryota.
ENOG410XPMZ. LUCA.
GeneTreeiENSGT00550000074508.
HOGENOMiHOG000231699.
HOVERGENiHBG069548.
InParanoidiQ91Z31.
KOiK14948.
OMAiNLGESHH.
OrthoDBiEOG091G0Z5R.
PhylomeDBiQ91Z31.
TreeFamiTF319824.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L/PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91Z31-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGIVTEVAV GVKRGSDELL SGSVLSSPNS NMSGMVVTAN GNDSKKFKGE
60 70 80 90 100
DKMDGAPSRV LHIRKLPGEV TETEVIALGL PFGKVTNILM LKGKNQAFLE
110 120 130 140 150
LATEEAAITM VNYYSAVTPH LRNQPIYIQY SNHKELKTDN TLNQRAQVVL
160 170 180 190 200
QAVTAVQTAN TPLSGTTVSE SAVTPAQSPV LRIIIDNMYY PVTLDVLHQI
210 220 230 240 250
FSKFGAVLKI ITFTKNNQFQ ALLQYGDPVN AQQAKLALDG QNIYNACCTL
260 270 280 290 300
RIDFSKLVNL NVKYNNDKSR DYTRPDLPSG DGQPALDPAI AAAFAKETSL
310 320 330 340 350
LAVPGALSPL AIPNAAAAAA AAAAGRVGMP GVSAGGNTVL LVSNLNEEMV
360 370 380 390 400
TPQSLFTLFG VYGDVQRVKI LYNKKDSALI QMADGNQSQL AMNHLNGQKM
410 420 430 440 450
YGKIIRVTLS KHQTVQLPRE GLDDQGLTKD FGNSPLHRFK KPGSKNFQNI
460 470 480 490 500
FPPSATLHLS NIPPSVAEED LRTLFANTGG TVKAFKFFQD HKMALLQMAT
510 520 530
VEEAIQALID LHNYNLGENH HLRVSFSKST I
Length:531
Mass (Da):57,489
Last modified:April 18, 2006 - v2
Checksum:i3A93CA4E3E1D6AA3
GO
Isoform 2 (identifier: Q91Z31-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     489-489: Q → QR

Show »
Length:532
Mass (Da):57,645
Checksum:i732A3CC8C6D47512
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti93 – 931G → E in AAH10255 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei489 – 4891Q → QR in isoform 2. 2 PublicationsVSP_018019

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095718 mRNA. Translation: AAF21807.2.
AK013734 mRNA. Translation: BAB28977.1.
AK137348 mRNA. Translation: BAE23317.1.
BC010255 mRNA. Translation: AAH10255.1.
AY333752 Genomic DNA. Translation: AAQ01150.1.
BK000527 mRNA. Translation: DAA00061.1.
CCDSiCCDS38616.1. [Q91Z31-2]
CCDS80010.1. [Q91Z31-1]
RefSeqiNP_001297640.1. NM_001310711.1. [Q91Z31-1]
NP_062423.1. NM_019550.2. [Q91Z31-2]
UniGeneiMm.29966.

Genome annotation databases

EnsembliENSMUST00000029780; ENSMUSP00000029780; ENSMUSG00000028134. [Q91Z31-2]
ENSMUST00000200097; ENSMUSP00000143510; ENSMUSG00000028134. [Q91Z31-1]
GeneIDi56195.
KEGGimmu:56195.
UCSCiuc008rdi.1. mouse. [Q91Z31-1]
uc008rdk.2. mouse. [Q91Z31-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF095718 mRNA. Translation: AAF21807.2.
AK013734 mRNA. Translation: BAB28977.1.
AK137348 mRNA. Translation: BAE23317.1.
BC010255 mRNA. Translation: AAH10255.1.
AY333752 Genomic DNA. Translation: AAQ01150.1.
BK000527 mRNA. Translation: DAA00061.1.
CCDSiCCDS38616.1. [Q91Z31-2]
CCDS80010.1. [Q91Z31-1]
RefSeqiNP_001297640.1. NM_001310711.1. [Q91Z31-1]
NP_062423.1. NM_019550.2. [Q91Z31-2]
UniGeneiMm.29966.

3D structure databases

ProteinModelPortaliQ91Z31.
SMRiQ91Z31. Positions 51-138, 177-281, 325-531.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207834. 3 interactions.
IntActiQ91Z31. 3 interactions.
MINTiMINT-4130609.
STRINGi10090.ENSMUSP00000029780.

PTM databases

iPTMnetiQ91Z31.
PhosphoSiteiQ91Z31.

Proteomic databases

MaxQBiQ91Z31.
PaxDbiQ91Z31.
PeptideAtlasiQ91Z31.
PRIDEiQ91Z31.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029780; ENSMUSP00000029780; ENSMUSG00000028134. [Q91Z31-2]
ENSMUST00000200097; ENSMUSP00000143510; ENSMUSG00000028134. [Q91Z31-1]
GeneIDi56195.
KEGGimmu:56195.
UCSCiuc008rdi.1. mouse. [Q91Z31-1]
uc008rdk.2. mouse. [Q91Z31-2]

Organism-specific databases

CTDi58155.
MGIiMGI:1860489. Ptbp2.

Phylogenomic databases

eggNOGiKOG1190. Eukaryota.
ENOG410XPMZ. LUCA.
GeneTreeiENSGT00550000074508.
HOGENOMiHOG000231699.
HOVERGENiHBG069548.
InParanoidiQ91Z31.
KOiK14948.
OMAiNLGESHH.
OrthoDBiEOG091G0Z5R.
PhylomeDBiQ91Z31.
TreeFamiTF319824.

Miscellaneous databases

ChiTaRSiPtbp2. mouse.
PROiQ91Z31.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028134.
CleanExiMM_PTBP2.
ExpressionAtlasiQ91Z31. baseline and differential.
GenevisibleiQ91Z31. MM.

Family and domain databases

Gene3Di3.30.70.330. 4 hits.
InterProiIPR006536. HnRNP-L/PTB.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 4 hits.
TIGRFAMsiTIGR01649. hnRNP-L_PTB. 1 hit.
PROSITEiPS50102. RRM. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTBP2_MOUSE
AccessioniPrimary (citable) accession number: Q91Z31
Secondary accession number(s): Q7TNW7
, Q9CUW2, Q9QYC2, Q9R0V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: April 18, 2006
Last modified: September 7, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.