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Protein

Beta-1,4-galactosyltransferase 3

Gene

B4galt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi199ManganeseBy similarity1
Binding sitei228UDP-alpha-D-galactoseBy similarity1
Binding sitei260UDP-alpha-D-galactoseBy similarity1
Metal bindingi293Manganese; via tele nitrogenBy similarity1
Binding sitei305N-acetyl-D-glucosamineBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2022854. Keratan sulfate biosynthesis.
R-MMU-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 3 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 3
Short name:
Beta4Gal-T3
Short name:
b4Gal-T3
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
Including the following 3 domains:
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
Gene namesi
Name:B4galt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1928767. B4galt3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 10CytoplasmicSequence analysis10
Transmembranei11 – 31Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini32 – 395LumenalSequence analysisAdd BLAST364

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805381 – 395Beta-1,4-galactosyltransferase 3Add BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi57N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi79 ↔ 121By similarity
Glycosylationi168N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi192 ↔ 211By similarity
Glycosylationi339N-linked (GlcNAc...)1 Publication1
Glycosylationi387N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ91YY2.
PaxDbiQ91YY2.
PRIDEiQ91YY2.

PTM databases

PhosphoSitePlusiQ91YY2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000052423.
ExpressionAtlasiQ91YY2. baseline and differential.
GenevisibleiQ91YY2. MM.

Interactioni

Protein-protein interaction databases

IntActiQ91YY2. 1 interactor.
MINTiMINT-4122097.
STRINGi10090.ENSMUSP00000066353.

Structurei

3D structure databases

ProteinModelPortaliQ91YY2.
SMRiQ91YY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni132 – 136UDP-alpha-D-galactose bindingBy similarity5
Regioni171 – 173UDP-alpha-D-galactose bindingBy similarity3
Regioni198 – 199UDP-alpha-D-galactose bindingBy similarity2
Regioni262 – 265N-acetyl-D-glucosamine bindingBy similarity4
Regioni293 – 295UDP-alpha-D-galactose bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3916. Eukaryota.
ENOG410ZYYA. LUCA.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ91YY2.
KOiK07968.
OMAiIGFQFAF.
OrthoDBiEOG091G0P66.
PhylomeDBiQ91YY2.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q91YY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DPGPTFDYSH
60 70 80 90 100
PHDVYSNLSH LPAAPGAAGA PPAQALPYCP ERSPFLVGPV SVSFSPVPSL
110 120 130 140 150
AEIVERNPRV ESGGRYRPAG CEPRSRTAII VPHRAREHHL RLLLYHLHPF
160 170 180 190 200
LQRQQLAYGI YVIHQAGNGT FNRAKLLNVG VREALRDEEW DCLFLHDVDL
210 220 230 240 250
LPENDHNLYV CDPRGPRHVA VAMNKFGYSL PYPQYFGGVS ALTPDQYLKM
260 270 280 290 300
NGFPNEYWGW GGEDDDIATR VRLAGMKISR PPTSVGHYKM VKHRGDKGNE
310 320 330 340 350
ENPHRFDLLV RTQNSWTQDG MNSLTYRLLA RELGPLYTNI TADIGTDPRG
360 370 380 390
PRSPSGPRYP PGSSQAFRQE MLQRRPPARP GPLPTANHTA PRGSH
Length:395
Mass (Da):44,084
Last modified:December 1, 2001 - v1
Checksum:i16401B033EEB4B21
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti353S → A in AAF22221 (PubMed:9597550).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142671 mRNA. Translation: AAF22221.1.
BC013619 mRNA. Translation: AAH13619.1.
CCDSiCCDS15486.1.
RefSeqiNP_065604.2. NM_020579.2.
UniGeneiMm.274011.

Genome annotation databases

EnsembliENSMUST00000064272; ENSMUSP00000066353; ENSMUSG00000052423.
ENSMUST00000111313; ENSMUSP00000106945; ENSMUSG00000052423.
GeneIDi57370.
KEGGimmu:57370.
UCSCiuc007dnp.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

b4GalT3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142671 mRNA. Translation: AAF22221.1.
BC013619 mRNA. Translation: AAH13619.1.
CCDSiCCDS15486.1.
RefSeqiNP_065604.2. NM_020579.2.
UniGeneiMm.274011.

3D structure databases

ProteinModelPortaliQ91YY2.
SMRiQ91YY2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91YY2. 1 interactor.
MINTiMINT-4122097.
STRINGi10090.ENSMUSP00000066353.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSitePlusiQ91YY2.

Proteomic databases

MaxQBiQ91YY2.
PaxDbiQ91YY2.
PRIDEiQ91YY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064272; ENSMUSP00000066353; ENSMUSG00000052423.
ENSMUST00000111313; ENSMUSP00000106945; ENSMUSG00000052423.
GeneIDi57370.
KEGGimmu:57370.
UCSCiuc007dnp.2. mouse.

Organism-specific databases

CTDi8703.
MGIiMGI:1928767. B4galt3.

Phylogenomic databases

eggNOGiKOG3916. Eukaryota.
ENOG410ZYYA. LUCA.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ91YY2.
KOiK07968.
OMAiIGFQFAF.
OrthoDBiEOG091G0P66.
PhylomeDBiQ91YY2.
TreeFamiTF312834.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-2022854. Keratan sulfate biosynthesis.
R-MMU-975577. N-Glycan antennae elongation.

Miscellaneous databases

ChiTaRSiB4galt3. mouse.
PROiQ91YY2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052423.
ExpressionAtlasiQ91YY2. baseline and differential.
GenevisibleiQ91YY2. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB4GT3_MOUSE
AccessioniPrimary (citable) accession number: Q91YY2
Secondary accession number(s): Q9QY13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.