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Protein

Beta-1,4-galactosyltransferase 3

Gene

B4galt3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi199 – 1991ManganeseBy similarity
Binding sitei228 – 2281UDP-alpha-D-galactoseBy similarity
Binding sitei260 – 2601UDP-alpha-D-galactoseBy similarity
Metal bindingi293 – 2931Manganese; via tele nitrogenBy similarity
Binding sitei305 – 3051N-acetyl-D-glucosamineBy similarity

GO - Molecular functioni

  1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. N-acetyllactosamine synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198565. N-Glycan antennae elongation.
REACT_198578. Keratan sulfate biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 3 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 3
Short name:
Beta4Gal-T3
Short name:
b4Gal-T3
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
Including the following 3 domains:
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
Gene namesi
Name:B4galt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1928767. B4galt3.

Subcellular locationi

Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
Note: Trans cisternae of Golgi stack.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010CytoplasmicSequence Analysis
Transmembranei11 – 3121Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini32 – 395364LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. extracellular vesicular exosome Source: MGI
  3. Golgi apparatus Source: MGI
  4. Golgi cisterna membrane Source: UniProtKB-SubCell
  5. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Beta-1,4-galactosyltransferase 3PRO_0000080538Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi79 ↔ 121By similarity
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi192 ↔ 211By similarity
Glycosylationi339 – 3391N-linked (GlcNAc...)1 Publication
Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ91YY2.
PaxDbiQ91YY2.
PRIDEiQ91YY2.

PTM databases

PhosphoSiteiQ91YY2.

Expressioni

Gene expression databases

BgeeiQ91YY2.
ExpressionAtlasiQ91YY2. baseline and differential.
GenevestigatoriQ91YY2.

Interactioni

Protein-protein interaction databases

IntActiQ91YY2. 1 interaction.
MINTiMINT-4122097.

Structurei

3D structure databases

ProteinModelPortaliQ91YY2.
SMRiQ91YY2. Positions 76-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni132 – 1365UDP-alpha-D-galactose bindingBy similarity
Regioni171 – 1733UDP-alpha-D-galactose bindingBy similarity
Regioni198 – 1992UDP-alpha-D-galactose bindingBy similarity
Regioni262 – 2654N-acetyl-D-glucosamine bindingBy similarity
Regioni293 – 2953UDP-alpha-D-galactose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ91YY2.
KOiK07968.
OMAiLEPLYTN.
OrthoDBiEOG7060R0.
PhylomeDBiQ91YY2.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q91YY2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DPGPTFDYSH
60 70 80 90 100
PHDVYSNLSH LPAAPGAAGA PPAQALPYCP ERSPFLVGPV SVSFSPVPSL
110 120 130 140 150
AEIVERNPRV ESGGRYRPAG CEPRSRTAII VPHRAREHHL RLLLYHLHPF
160 170 180 190 200
LQRQQLAYGI YVIHQAGNGT FNRAKLLNVG VREALRDEEW DCLFLHDVDL
210 220 230 240 250
LPENDHNLYV CDPRGPRHVA VAMNKFGYSL PYPQYFGGVS ALTPDQYLKM
260 270 280 290 300
NGFPNEYWGW GGEDDDIATR VRLAGMKISR PPTSVGHYKM VKHRGDKGNE
310 320 330 340 350
ENPHRFDLLV RTQNSWTQDG MNSLTYRLLA RELGPLYTNI TADIGTDPRG
360 370 380 390
PRSPSGPRYP PGSSQAFRQE MLQRRPPARP GPLPTANHTA PRGSH
Length:395
Mass (Da):44,084
Last modified:December 1, 2001 - v1
Checksum:i16401B033EEB4B21
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti353 – 3531S → A in AAF22221. (PubMed:9597550)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142671 mRNA. Translation: AAF22221.1.
BC013619 mRNA. Translation: AAH13619.1.
CCDSiCCDS15486.1.
RefSeqiNP_065604.2. NM_020579.2.
XP_006497014.1. XM_006496951.1.
UniGeneiMm.274011.

Genome annotation databases

EnsembliENSMUST00000064272; ENSMUSP00000066353; ENSMUSG00000052423.
ENSMUST00000111313; ENSMUSP00000106945; ENSMUSG00000052423.
GeneIDi57370.
KEGGimmu:57370.
UCSCiuc007dnp.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

b4GalT3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142671 mRNA. Translation: AAF22221.1.
BC013619 mRNA. Translation: AAH13619.1.
CCDSiCCDS15486.1.
RefSeqiNP_065604.2. NM_020579.2.
XP_006497014.1. XM_006496951.1.
UniGeneiMm.274011.

3D structure databases

ProteinModelPortaliQ91YY2.
SMRiQ91YY2. Positions 76-346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ91YY2. 1 interaction.
MINTiMINT-4122097.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSiteiQ91YY2.

Proteomic databases

MaxQBiQ91YY2.
PaxDbiQ91YY2.
PRIDEiQ91YY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064272; ENSMUSP00000066353; ENSMUSG00000052423.
ENSMUST00000111313; ENSMUSP00000106945; ENSMUSG00000052423.
GeneIDi57370.
KEGGimmu:57370.
UCSCiuc007dnp.2. mouse.

Organism-specific databases

CTDi8703.
MGIiMGI:1928767. B4galt3.

Phylogenomic databases

eggNOGiNOG327897.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ91YY2.
KOiK07968.
OMAiLEPLYTN.
OrthoDBiEOG7060R0.
PhylomeDBiQ91YY2.
TreeFamiTF312834.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_198565. N-Glycan antennae elongation.
REACT_198578. Keratan sulfate biosynthesis.

Miscellaneous databases

ChiTaRSiB4galt3. mouse.
NextBioi313744.
PROiQ91YY2.
SOURCEiSearch...

Gene expression databases

BgeeiQ91YY2.
ExpressionAtlasiQ91YY2. baseline and differential.
GenevestigatoriQ91YY2.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The expanding beta 4-galactosyltransferase gene family: messages from the databanks."
    Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L.
    Glycobiology 8:517-526(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-339.

Entry informationi

Entry nameiB4GT3_MOUSE
AccessioniPrimary (citable) accession number: Q91YY2
Secondary accession number(s): Q9QY13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: December 1, 2001
Last modified: February 4, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.