Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q91YY2

- B4GT3_MOUSE

UniProt

Q91YY2 - B4GT3_MOUSE

Protein

Beta-1,4-galactosyltransferase 3

Gene

B4galt3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

    Catalytic activityi

    UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
    UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi199 – 1991ManganeseBy similarity
    Binding sitei228 – 2281UDP-alpha-D-galactoseBy similarity
    Binding sitei260 – 2601UDP-alpha-D-galactoseBy similarity
    Metal bindingi293 – 2931Manganese; via tele nitrogenBy similarity
    Binding sitei305 – 3051N-acetyl-D-glucosamineBy similarity

    GO - Molecular functioni

    1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. N-acetyllactosamine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198565. N-Glycan antennae elongation.
    REACT_198578. Keratan sulfate biosynthesis.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 3 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 3
    Short name:
    Beta4Gal-T3
    Short name:
    b4Gal-T3
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
    Including the following 3 domains:
    N-acetyllactosamine synthase (EC:2.4.1.90)
    Alternative name(s):
    Nal synthase
    Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
    Gene namesi
    Name:B4galt3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1928767. B4galt3.

    Subcellular locationi

    Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Trans cisternae of Golgi stack.By similarity

    GO - Cellular componenti

    1. Golgi cisterna membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 395395Beta-1,4-galactosyltransferase 3PRO_0000080538Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi57 – 571N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi79 ↔ 121By similarity
    Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi192 ↔ 211By similarity
    Glycosylationi339 – 3391N-linked (GlcNAc...)1 Publication
    Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ91YY2.
    PRIDEiQ91YY2.

    PTM databases

    PhosphoSiteiQ91YY2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ91YY2.
    BgeeiQ91YY2.
    GenevestigatoriQ91YY2.

    Interactioni

    Protein-protein interaction databases

    IntActiQ91YY2. 1 interaction.
    MINTiMINT-4122097.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91YY2.
    SMRiQ91YY2. Positions 76-346.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1010CytoplasmicSequence Analysis
    Topological domaini32 – 395364LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei11 – 3121Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni132 – 1365UDP-alpha-D-galactose bindingBy similarity
    Regioni171 – 1733UDP-alpha-D-galactose bindingBy similarity
    Regioni198 – 1992UDP-alpha-D-galactose bindingBy similarity
    Regioni262 – 2654N-acetyl-D-glucosamine bindingBy similarity
    Regioni293 – 2953UDP-alpha-D-galactose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG327897.
    GeneTreeiENSGT00750000117412.
    HOGENOMiHOG000231027.
    HOVERGENiHBG058334.
    InParanoidiQ91YY2.
    KOiK07968.
    OMAiALFGREQ.
    OrthoDBiEOG7060R0.
    PhylomeDBiQ91YY2.
    TreeFamiTF312834.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q91YY2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DPGPTFDYSH    50
    PHDVYSNLSH LPAAPGAAGA PPAQALPYCP ERSPFLVGPV SVSFSPVPSL 100
    AEIVERNPRV ESGGRYRPAG CEPRSRTAII VPHRAREHHL RLLLYHLHPF 150
    LQRQQLAYGI YVIHQAGNGT FNRAKLLNVG VREALRDEEW DCLFLHDVDL 200
    LPENDHNLYV CDPRGPRHVA VAMNKFGYSL PYPQYFGGVS ALTPDQYLKM 250
    NGFPNEYWGW GGEDDDIATR VRLAGMKISR PPTSVGHYKM VKHRGDKGNE 300
    ENPHRFDLLV RTQNSWTQDG MNSLTYRLLA RELGPLYTNI TADIGTDPRG 350
    PRSPSGPRYP PGSSQAFRQE MLQRRPPARP GPLPTANHTA PRGSH 395
    Length:395
    Mass (Da):44,084
    Last modified:December 1, 2001 - v1
    Checksum:i16401B033EEB4B21
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti353 – 3531S → A in AAF22221. (PubMed:9597550)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF142671 mRNA. Translation: AAF22221.1.
    BC013619 mRNA. Translation: AAH13619.1.
    CCDSiCCDS15486.1.
    RefSeqiNP_065604.2. NM_020579.2.
    XP_006497014.1. XM_006496951.1.
    UniGeneiMm.274011.

    Genome annotation databases

    EnsembliENSMUST00000064272; ENSMUSP00000066353; ENSMUSG00000052423.
    ENSMUST00000111313; ENSMUSP00000106945; ENSMUSG00000052423.
    GeneIDi57370.
    KEGGimmu:57370.
    UCSCiuc007dnp.2. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    b4GalT3

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF142671 mRNA. Translation: AAF22221.1 .
    BC013619 mRNA. Translation: AAH13619.1 .
    CCDSi CCDS15486.1.
    RefSeqi NP_065604.2. NM_020579.2.
    XP_006497014.1. XM_006496951.1.
    UniGenei Mm.274011.

    3D structure databases

    ProteinModelPortali Q91YY2.
    SMRi Q91YY2. Positions 76-346.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q91YY2. 1 interaction.
    MINTi MINT-4122097.

    Protein family/group databases

    CAZyi GT7. Glycosyltransferase Family 7.

    PTM databases

    PhosphoSitei Q91YY2.

    Proteomic databases

    PaxDbi Q91YY2.
    PRIDEi Q91YY2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000064272 ; ENSMUSP00000066353 ; ENSMUSG00000052423 .
    ENSMUST00000111313 ; ENSMUSP00000106945 ; ENSMUSG00000052423 .
    GeneIDi 57370.
    KEGGi mmu:57370.
    UCSCi uc007dnp.2. mouse.

    Organism-specific databases

    CTDi 8703.
    MGIi MGI:1928767. B4galt3.

    Phylogenomic databases

    eggNOGi NOG327897.
    GeneTreei ENSGT00750000117412.
    HOGENOMi HOG000231027.
    HOVERGENi HBG058334.
    InParanoidi Q91YY2.
    KOi K07968.
    OMAi ALFGREQ.
    OrthoDBi EOG7060R0.
    PhylomeDBi Q91YY2.
    TreeFami TF312834.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_198565. N-Glycan antennae elongation.
    REACT_198578. Keratan sulfate biosynthesis.

    Miscellaneous databases

    NextBioi 313744.
    PROi Q91YY2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q91YY2.
    Bgeei Q91YY2.
    Genevestigatori Q91YY2.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR19300. PTHR19300. 1 hit.
    Pfami PF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view ]
    PRINTSi PR02050. B14GALTRFASE.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The expanding beta 4-galactosyltransferase gene family: messages from the databanks."
      Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L.
      Glycobiology 8:517-526(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    3. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-339.

    Entry informationi

    Entry nameiB4GT3_MOUSE
    AccessioniPrimary (citable) accession number: Q91YY2
    Secondary accession number(s): Q9QY13
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3