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Q91YY2

- B4GT3_MOUSE

UniProt

Q91YY2 - B4GT3_MOUSE

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Protein

Beta-1,4-galactosyltransferase 3

Gene

B4galt3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi199 – 1991ManganeseBy similarity
Binding sitei228 – 2281UDP-alpha-D-galactoseBy similarity
Binding sitei260 – 2601UDP-alpha-D-galactoseBy similarity
Metal bindingi293 – 2931Manganese; via tele nitrogenBy similarity
Binding sitei305 – 3051N-acetyl-D-glucosamineBy similarity

GO - Molecular functioni

  1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. N-acetyllactosamine synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198565. N-Glycan antennae elongation.
REACT_198578. Keratan sulfate biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 3 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 3
Short name:
Beta4Gal-T3
Short name:
b4Gal-T3
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
Including the following 3 domains:
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
Gene namesi
Name:B4galt3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1928767. B4galt3.

Subcellular locationi

Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
Note: Trans cisternae of Golgi stack.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1010CytoplasmicSequence Analysis
Transmembranei11 – 3121Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini32 – 395364LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. Golgi apparatus Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Beta-1,4-galactosyltransferase 3PRO_0000080538Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi79 ↔ 121By similarity
Glycosylationi168 – 1681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi192 ↔ 211By similarity
Glycosylationi339 – 3391N-linked (GlcNAc...)1 Publication
Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ91YY2.
PaxDbiQ91YY2.
PRIDEiQ91YY2.

PTM databases

PhosphoSiteiQ91YY2.

Expressioni

Gene expression databases

BgeeiQ91YY2.
ExpressionAtlasiQ91YY2. baseline and differential.
GenevestigatoriQ91YY2.

Interactioni

Protein-protein interaction databases

IntActiQ91YY2. 1 interaction.
MINTiMINT-4122097.

Structurei

3D structure databases

ProteinModelPortaliQ91YY2.
SMRiQ91YY2. Positions 76-346.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni132 – 1365UDP-alpha-D-galactose bindingBy similarity
Regioni171 – 1733UDP-alpha-D-galactose bindingBy similarity
Regioni198 – 1992UDP-alpha-D-galactose bindingBy similarity
Regioni262 – 2654N-acetyl-D-glucosamine bindingBy similarity
Regioni293 – 2953UDP-alpha-D-galactose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ91YY2.
KOiK07968.
OMAiALFGREQ.
OrthoDBiEOG7060R0.
PhylomeDBiQ91YY2.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q91YY2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DPGPTFDYSH
60 70 80 90 100
PHDVYSNLSH LPAAPGAAGA PPAQALPYCP ERSPFLVGPV SVSFSPVPSL
110 120 130 140 150
AEIVERNPRV ESGGRYRPAG CEPRSRTAII VPHRAREHHL RLLLYHLHPF
160 170 180 190 200
LQRQQLAYGI YVIHQAGNGT FNRAKLLNVG VREALRDEEW DCLFLHDVDL
210 220 230 240 250
LPENDHNLYV CDPRGPRHVA VAMNKFGYSL PYPQYFGGVS ALTPDQYLKM
260 270 280 290 300
NGFPNEYWGW GGEDDDIATR VRLAGMKISR PPTSVGHYKM VKHRGDKGNE
310 320 330 340 350
ENPHRFDLLV RTQNSWTQDG MNSLTYRLLA RELGPLYTNI TADIGTDPRG
360 370 380 390
PRSPSGPRYP PGSSQAFRQE MLQRRPPARP GPLPTANHTA PRGSH
Length:395
Mass (Da):44,084
Last modified:December 1, 2001 - v1
Checksum:i16401B033EEB4B21
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti353 – 3531S → A in AAF22221. (PubMed:9597550)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142671 mRNA. Translation: AAF22221.1.
BC013619 mRNA. Translation: AAH13619.1.
CCDSiCCDS15486.1.
RefSeqiNP_065604.2. NM_020579.2.
XP_006497014.1. XM_006496951.1.
UniGeneiMm.274011.

Genome annotation databases

EnsembliENSMUST00000064272; ENSMUSP00000066353; ENSMUSG00000052423.
ENSMUST00000111313; ENSMUSP00000106945; ENSMUSG00000052423.
GeneIDi57370.
KEGGimmu:57370.
UCSCiuc007dnp.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

b4GalT3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142671 mRNA. Translation: AAF22221.1 .
BC013619 mRNA. Translation: AAH13619.1 .
CCDSi CCDS15486.1.
RefSeqi NP_065604.2. NM_020579.2.
XP_006497014.1. XM_006496951.1.
UniGenei Mm.274011.

3D structure databases

ProteinModelPortali Q91YY2.
SMRi Q91YY2. Positions 76-346.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q91YY2. 1 interaction.
MINTi MINT-4122097.

Protein family/group databases

CAZyi GT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSitei Q91YY2.

Proteomic databases

MaxQBi Q91YY2.
PaxDbi Q91YY2.
PRIDEi Q91YY2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000064272 ; ENSMUSP00000066353 ; ENSMUSG00000052423 .
ENSMUST00000111313 ; ENSMUSP00000106945 ; ENSMUSG00000052423 .
GeneIDi 57370.
KEGGi mmu:57370.
UCSCi uc007dnp.2. mouse.

Organism-specific databases

CTDi 8703.
MGIi MGI:1928767. B4galt3.

Phylogenomic databases

eggNOGi NOG327897.
GeneTreei ENSGT00760000119140.
HOGENOMi HOG000231027.
HOVERGENi HBG058334.
InParanoidi Q91YY2.
KOi K07968.
OMAi ALFGREQ.
OrthoDBi EOG7060R0.
PhylomeDBi Q91YY2.
TreeFami TF312834.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_198565. N-Glycan antennae elongation.
REACT_198578. Keratan sulfate biosynthesis.

Miscellaneous databases

ChiTaRSi B4galt3. mouse.
NextBioi 313744.
PROi Q91YY2.
SOURCEi Search...

Gene expression databases

Bgeei Q91YY2.
ExpressionAtlasi Q91YY2. baseline and differential.
Genevestigatori Q91YY2.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR19300. PTHR19300. 1 hit.
Pfami PF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view ]
PRINTSi PR02050. B14GALTRFASE.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The expanding beta 4-galactosyltransferase gene family: messages from the databanks."
    Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L.
    Glycobiology 8:517-526(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-339.

Entry informationi

Entry nameiB4GT3_MOUSE
AccessioniPrimary (citable) accession number: Q91YY2
Secondary accession number(s): Q9QY13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: December 1, 2001
Last modified: November 26, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3