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Protein

Protein THEMIS2

Gene

Themis2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May constitute a control point in macrophage inflammatory response, promoting LPS-induced TLR4-mediated TNF production.1 Publication

GO - Biological processi

  • inflammatory response Source: UniProtKB-KW
  • T cell receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Biological processi

Immunity, Inflammatory response

Names & Taxonomyi

Protein namesi
Recommended name:
Protein THEMIS2
Alternative name(s):
Induced by contact to basement membrane 1 protein
Short name:
Protein ICB-1
Thymocyte-expressed molecule involved in selection protein 2
Gene namesi
Name:Themis2
Synonyms:Icb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2446213. Themis2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi552P → A: Drastic decrease in GRB2-binding, but no effect on VAV-binding; when associated with A-555. Partial loss of enhancement of LPS-induced TNF production; when associated with A-555. 1
Mutagenesisi555P → A: Drastic decrease in GRB2-binding, but no effect on VAV-binding; when associated with A-552. Partial loss of enhancement of LPS-induced TNF production; when associated with A-552. 1
Mutagenesisi660Y → F: Loss of phosphorylation. Loss of LYN-binding. Slight decrease in GRB2-binding. No effect on VAV-binding. Partial loss of enhancement of LPS-induced TNF production. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000841441 – 663Protein THEMIS2Add BLAST663

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei596PhosphothreonineBy similarity1
Modified residuei660Phosphotyrosine1 Publication1

Post-translational modificationi

Phosphorylation at Tyr-660 is induced by LPS.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ91YX0.
PaxDbiQ91YX0.
PeptideAtlasiQ91YX0.
PRIDEiQ91YX0.

PTM databases

iPTMnetiQ91YX0.
PhosphoSitePlusiQ91YX0.

Expressioni

Tissue specificityi

Restricted to B-cells, macrophages and dendritic cells. Down-regulated in splenocytes of mice developing arthritis in a collagen-induced model, not in those of mice failing to develop the disease. Transiently down-regulated in splenocytes of mice infected with influenza virus.1 Publication

Developmental stagei

Up-regulated during the differentiation of bone marrow precursors into macrophages.1 Publication

Inductioni

Up-regulated by pro-inflammatory stimuli, such as IFNG (at protein level). Down-regulated by anti-inflammatory stimuli, such as TGFB1 and dexamethasone (at protein level).

Gene expression databases

BgeeiENSMUSG00000037731.
CleanExiMM_BC013712.
GenevisibleiQ91YX0. MM.

Interactioni

Subunit structurei

When phosphorylated, interacts with LYN. Interacts with VAV1 and GRB2.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036945.

Structurei

3D structure databases

ProteinModelPortaliQ91YX0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 237CABIT 1Add BLAST236
Regioni238 – 515CABIT 2Add BLAST278

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi403 – 419Glu-richAdd BLAST17

Sequence similaritiesi

Belongs to the themis family.Curated

Phylogenomic databases

eggNOGiENOG410IF5X. Eukaryota.
ENOG4110SKT. LUCA.
GeneTreeiENSGT00530000063770.
HOGENOMiHOG000112995.
InParanoidiQ91YX0.
OMAiFMSTHRI.
OrthoDBiEOG091G02V6.
PhylomeDBiQ91YX0.
TreeFamiTF333479.

Family and domain databases

InterProiIPR025946. CABIT_dom.
[Graphical view]
PfamiPF12736. CABIT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91YX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPVPLQDFV SGLDPTSLPR VLRVCSGVYF EGSVYELFGN ECCLSTGDLI
60 70 80 90 100
KVTHVQLQKV VCEYPETGQT LELNPNFTGL FSPLTSLRSY RTLEDLVSAM
110 120 130 140 150
PQNSTRWPIY FKSTQRIVTK ASVVPEDQPL RLEAVEIHHG IRYARCVQVS
160 170 180 190 200
KTKELLHLPL SQKGPFWRCK PSAPQTLHQI LQDPALKDLT LSCPSLPWNS
210 220 230 240 250
VILKPQYMLQ AIMHMRSSIV KIPSTLEVEV EDVTASSQHI HFFKPLRLSE
260 270 280 290 300
VLAGGGPFPL TTEILEVPEG PPVFLSPWVS FLRKGQRLCI YGPASPSWRV
310 320 330 340 350
VASSKSRKVP RYFMLSGAYQ GKLKRRPREF STAYDLLGAL QPGRPLRVVA
360 370 380 390 400
TKDCDGNEEE NPDFSFLAVG DRLEVLRSGQ VCGTKGQDID VLVCQRLSEQ
410 420 430 440 450
SGEEEEDLEE IEDEAEDKEQ ILLPLYLSGS FVEEVNDSRR YNLVDLTAQY
460 470 480 490 500
SLPCEVKVVT KDTRHPTDPL ASFPGLRLEE KLTEPFLVVS LDSQPEMCFE
510 520 530 540 550
IPPRWLDLTV VEAEGQPAQV ARPLSIAPVE ELSEAFYYSL RKLPASESQA
560 570 580 590 600
PPPRPPKSQG INKKQQNIQS CKESSVKPQV VEPQKSCPQP QLKAKTLEAL
610 620 630 640 650
PKNKSNVYSK ISVHKKDRKP NPQTQNSVLS MKPKTSSSLG KHSTMESHLL
660
PDPDMDDHDY EEI
Length:663
Mass (Da):74,378
Last modified:August 30, 2005 - v2
Checksum:iC14C53B493D62A13
GO

Sequence cautioni

The sequence AAH13712 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK150471 mRNA. Translation: BAE29588.1.
AK153536 mRNA. Translation: BAE32073.1.
AK155536 mRNA. Translation: BAE33313.1.
AK171009 mRNA. Translation: BAE42180.1.
AL627130, AL671190 Genomic DNA. Translation: CAM24493.1.
AL671190, AL627130 Genomic DNA. Translation: CAM25193.1.
BC013712 mRNA. Translation: AAH13712.1. Different initiation.
BC151122 mRNA. Translation: AAI51123.1.
BC151126 mRNA. Translation: AAI51127.1.
CCDSiCCDS18735.1.
RefSeqiNP_001028480.1. NM_001033308.2.
UniGeneiMm.290912.

Genome annotation databases

EnsembliENSMUST00000045154; ENSMUSP00000036945; ENSMUSG00000037731.
GeneIDi230787.
KEGGimmu:230787.
UCSCiuc008vbv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK150471 mRNA. Translation: BAE29588.1.
AK153536 mRNA. Translation: BAE32073.1.
AK155536 mRNA. Translation: BAE33313.1.
AK171009 mRNA. Translation: BAE42180.1.
AL627130, AL671190 Genomic DNA. Translation: CAM24493.1.
AL671190, AL627130 Genomic DNA. Translation: CAM25193.1.
BC013712 mRNA. Translation: AAH13712.1. Different initiation.
BC151122 mRNA. Translation: AAI51123.1.
BC151126 mRNA. Translation: AAI51127.1.
CCDSiCCDS18735.1.
RefSeqiNP_001028480.1. NM_001033308.2.
UniGeneiMm.290912.

3D structure databases

ProteinModelPortaliQ91YX0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036945.

PTM databases

iPTMnetiQ91YX0.
PhosphoSitePlusiQ91YX0.

Proteomic databases

MaxQBiQ91YX0.
PaxDbiQ91YX0.
PeptideAtlasiQ91YX0.
PRIDEiQ91YX0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045154; ENSMUSP00000036945; ENSMUSG00000037731.
GeneIDi230787.
KEGGimmu:230787.
UCSCiuc008vbv.1. mouse.

Organism-specific databases

CTDi9473.
MGIiMGI:2446213. Themis2.

Phylogenomic databases

eggNOGiENOG410IF5X. Eukaryota.
ENOG4110SKT. LUCA.
GeneTreeiENSGT00530000063770.
HOGENOMiHOG000112995.
InParanoidiQ91YX0.
OMAiFMSTHRI.
OrthoDBiEOG091G02V6.
PhylomeDBiQ91YX0.
TreeFamiTF333479.

Miscellaneous databases

PROiQ91YX0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037731.
CleanExiMM_BC013712.
GenevisibleiQ91YX0. MM.

Family and domain databases

InterProiIPR025946. CABIT_dom.
[Graphical view]
PfamiPF12736. CABIT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHMS2_MOUSE
AccessioniPrimary (citable) accession number: Q91YX0
Secondary accession number(s): Q3U5K5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: August 30, 2005
Last modified: November 2, 2016
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.