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Q91YW3

- DNJC3_MOUSE

UniProt

Q91YW3 - DNJC3_MOUSE

Protein

DnaJ homolog subfamily C member 3

Gene

Dnajc3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.2 Publications

    GO - Molecular functioni

    1. chaperone binding Source: BHF-UCL
    2. misfolded protein binding Source: BHF-UCL
    3. protein binding Source: IntAct
    4. protein kinase inhibitor activity Source: MGI

    GO - Biological processi

    1. endoplasmic reticulum unfolded protein response Source: BHF-UCL
    2. negative regulation of protein kinase activity Source: MGI
    3. proteolysis involved in cellular protein catabolic process Source: BHF-UCL

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Unfolded protein response

    Enzyme and pathway databases

    ReactomeiREACT_106572. XBP1(S) activates chaperone genes.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DnaJ homolog subfamily C member 3
    Alternative name(s):
    Interferon-induced, double-stranded RNA-activated protein kinase inhibitor
    Protein kinase inhibitor of 58 kDa
    Short name:
    Protein kinase inhibitor p58
    Gene namesi
    Name:Dnajc3
    Synonyms:P58ipk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:107373. Dnajc3.

    Subcellular locationi

    Endoplasmic reticulum 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. cytosol Source: BHF-UCL
    3. endoplasmic reticulum lumen Source: BHF-UCL

    Keywords - Cellular componenti

    Endoplasmic reticulum

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi48 – 481L → D: Reduces binding affinity for misfolded proteins by 40%. 1 Publication
    Mutagenesisi71 – 711Y → A: Reduces binding affinity for misfolded proteins by 40%. 1 Publication
    Mutagenesisi75 – 751Y → H: Reduces binding affinity for misfolded proteins by 40%. 1 Publication
    Mutagenesisi104 – 1041F → A: Reduces binding affinity for misfolded proteins by 40%. 1 Publication
    Mutagenesisi186 – 1861W → A: Doesn't affect binding of misfolded proteins. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131Sequence AnalysisAdd
    BLAST
    Chaini32 – 504473DnaJ homolog subfamily C member 3PRO_0000071046Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi248 ↔ 258By similarity
    Disulfide bondi313 ↔ 329By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiQ91YW3.
    PaxDbiQ91YW3.
    PRIDEiQ91YW3.

    2D gel databases

    REPRODUCTION-2DPAGEQ91YW3.

    PTM databases

    PhosphoSiteiQ91YW3.

    Expressioni

    Tissue specificityi

    Widely expressed, with high level in the liver.1 Publication

    Inductioni

    Up-regulated during an endoplasmic reticulum stress.1 Publication

    Gene expression databases

    ArrayExpressiQ91YW3.
    BgeeiQ91YW3.
    GenevestigatoriQ91YW3.

    Interactioni

    Subunit structurei

    Interacts with EIF2AK2. Forms a trimeric complex with DNAJB1 and HSPA8. Interacts with PRKRIR/P52RIPK By similarity. Interacts with EIF2AK3.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HSPA5P110212EBI-8381770,EBI-354921From a different organism.

    Protein-protein interaction databases

    BioGridi781938. 1 interaction.
    IntActiQ91YW3. 4 interactions.
    MINTiMINT-1838284.

    Structurei

    Secondary structure

    1
    504
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi36 – 4914
    Helixi53 – 6614
    Helixi71 – 8414
    Helixi87 – 10014
    Helixi105 – 11814
    Helixi121 – 13212
    Helixi138 – 16629
    Helixi170 – 18314
    Helixi188 – 20013
    Helixi204 – 21512
    Helixi222 – 23514
    Helixi238 – 25114
    Helixi256 – 28025
    Helixi284 – 29714
    Helixi302 – 31817
    Helixi322 – 33514
    Helixi340 – 35213
    Helixi356 – 36712
    Helixi374 – 39118

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3IEGX-ray2.51A/B35-393[»]
    ProteinModelPortaliQ91YW3.
    SMRiQ91YW3. Positions 35-455.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ91YW3.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati37 – 7034TPR 1Add
    BLAST
    Repeati72 – 10433TPR 2Add
    BLAST
    Repeati105 – 13834TPR 3Add
    BLAST
    Repeati154 – 18734TPR 4Add
    BLAST
    Repeati188 – 22134TPR 5Add
    BLAST
    Repeati222 – 25534TPR 6Add
    BLAST
    Repeati268 – 30134TPR 7Add
    BLAST
    Repeati306 – 33934TPR 8Add
    BLAST
    Repeati340 – 37334TPR 9Add
    BLAST
    Domaini394 – 46269JPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni375 – 39319Flexible linkerBy similarityAdd
    BLAST

    Domaini

    The J domain mediates interaction with HSPA8.By similarity
    Binding to misfolded proteins is mediated by a hydrophobic patch forming a large groove within the first two TPR repeats.

    Sequence similaritiesi

    Contains 1 J domain.PROSITE-ProRule annotation
    Contains 9 TPR repeats.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal, TPR repeat

    Phylogenomic databases

    eggNOGiCOG0484.
    GeneTreeiENSGT00720000108760.
    HOGENOMiHOG000193351.
    HOVERGENiHBG053820.
    InParanoidiQ91YW3.
    KOiK09523.
    OMAiQSERRDY.
    OrthoDBiEOG70KGPH.
    PhylomeDBiQ91YW3.
    TreeFamiTF105162.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    1.25.40.10. 3 hits.
    InterProiIPR001623. DnaJ_domain.
    IPR026901. DNAJC3.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    IPR001440. TPR_1.
    IPR019734. TPR_repeat.
    [Graphical view]
    PANTHERiPTHR24078:SF165. PTHR24078:SF165. 1 hit.
    PfamiPF00226. DnaJ. 1 hit.
    PF00515. TPR_1. 2 hits.
    [Graphical view]
    PRINTSiPR00625. JDOMAIN.
    SMARTiSM00271. DnaJ. 1 hit.
    SM00028. TPR. 7 hits.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    PROSITEiPS50076. DNAJ_2. 1 hit.
    PS50005. TPR. 8 hits.
    PS50293. TPR_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q91YW3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVAPGSVGSR LGAVFPFLLV LVDLQYEGAE CGVNADVEKH LELGKKLLAA    50
    GQLADALSQF HAAVDGDPDN YIAYYRRATV FLAMGKSKAA LPDLTKVIAL 100
    KMDFTAARLQ RGHLLLKQGK LDEAEDDFKK VLKSNPSEQE EKEAESQLVK 150
    ADEMQRLRSQ ALDAFDGADY TAAITFLDKI LEVCVWDAEL RELRAECFIK 200
    EGEPRKAISD LKAASKLKSD NTEAFYKIST LYYQLGDHEL SLSEVRECLK 250
    LDQDHKRCFA HYKQVKKLNK LIESAEELIR DGRYTDATSK YESVMKTEPS 300
    VAEYTVRSKE RICHCFSKDE KPVEAIRICS EVLQMEPDNV NALKDRAEAY 350
    LIEEMYDEAI QDYEAAQEHN ENDQQIREGL EKAQRLLKQS QKRDYYKILG 400
    VKRNAKKQEI IKAYRKLALQ WHPDNFQNEE EKKKAEKKFI DIAAAKEVLS 450
    DPEMRKKFDD GEDPLDAESQ QGGGGNPFHR SWNSWQGFNP FSSGGPFRFK 500
    FHFN 504
    Length:504
    Mass (Da):57,464
    Last modified:December 1, 2001 - v1
    Checksum:i510C27782C6EDF66
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti89 – 913AAL → RRV in AAC52592. (PubMed:8666242)Curated
    Sequence conflicti134 – 1341S → C in AAC52592. (PubMed:8666242)Curated
    Sequence conflicti405 – 4051A → T in AAC52592. (PubMed:8666242)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U28423 mRNA. Translation: AAC52592.1.
    BC013766 mRNA. Translation: AAH13766.1.
    CCDSiCCDS37012.1.
    RefSeqiNP_032955.2. NM_008929.3.
    UniGeneiMm.12616.

    Genome annotation databases

    EnsembliENSMUST00000022734; ENSMUSP00000022734; ENSMUSG00000022136.
    GeneIDi100037258.
    KEGGimmu:100037258.
    UCSCiuc007uzd.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U28423 mRNA. Translation: AAC52592.1 .
    BC013766 mRNA. Translation: AAH13766.1 .
    CCDSi CCDS37012.1.
    RefSeqi NP_032955.2. NM_008929.3.
    UniGenei Mm.12616.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3IEG X-ray 2.51 A/B 35-393 [» ]
    ProteinModelPortali Q91YW3.
    SMRi Q91YW3. Positions 35-455.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 781938. 1 interaction.
    IntActi Q91YW3. 4 interactions.
    MINTi MINT-1838284.

    PTM databases

    PhosphoSitei Q91YW3.

    2D gel databases

    REPRODUCTION-2DPAGE Q91YW3.

    Proteomic databases

    MaxQBi Q91YW3.
    PaxDbi Q91YW3.
    PRIDEi Q91YW3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022734 ; ENSMUSP00000022734 ; ENSMUSG00000022136 .
    GeneIDi 100037258.
    KEGGi mmu:100037258.
    UCSCi uc007uzd.2. mouse.

    Organism-specific databases

    CTDi 5611.
    MGIi MGI:107373. Dnajc3.

    Phylogenomic databases

    eggNOGi COG0484.
    GeneTreei ENSGT00720000108760.
    HOGENOMi HOG000193351.
    HOVERGENi HBG053820.
    InParanoidi Q91YW3.
    KOi K09523.
    OMAi QSERRDY.
    OrthoDBi EOG70KGPH.
    PhylomeDBi Q91YW3.
    TreeFami TF105162.

    Enzyme and pathway databases

    Reactomei REACT_106572. XBP1(S) activates chaperone genes.

    Miscellaneous databases

    EvolutionaryTracei Q91YW3.
    NextBioi 444137.
    PROi Q91YW3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q91YW3.
    Bgeei Q91YW3.
    Genevestigatori Q91YW3.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    1.25.40.10. 3 hits.
    InterProi IPR001623. DnaJ_domain.
    IPR026901. DNAJC3.
    IPR013026. TPR-contain_dom.
    IPR011990. TPR-like_helical.
    IPR001440. TPR_1.
    IPR019734. TPR_repeat.
    [Graphical view ]
    PANTHERi PTHR24078:SF165. PTHR24078:SF165. 1 hit.
    Pfami PF00226. DnaJ. 1 hit.
    PF00515. TPR_1. 2 hits.
    [Graphical view ]
    PRINTSi PR00625. JDOMAIN.
    SMARTi SM00271. DnaJ. 1 hit.
    SM00028. TPR. 7 hits.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    PROSITEi PS50076. DNAJ_2. 1 hit.
    PS50005. TPR. 8 hits.
    PS50293. TPR_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression, and cellular localization of the oncogenic 58-kDa inhibitor of the RNA-activated human and mouse protein kinase."
      Korth M.J., Lyons C.N., Wambach M., Katze M.G.
      Gene 170:181-188(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary tumor.
    3. "Control of PERK eIF2alpha kinase activity by the endoplasmic reticulum stress-induced molecular chaperone P58IPK."
      Yan W., Frank C.L., Korth M.J., Sopher B.L., Novoa I., Ron D., Katze M.G.
      Proc. Natl. Acad. Sci. U.S.A. 99:15920-15925(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH EIF2AK3, INDUCTION.
    4. "Crystal structure of P58(IPK) TPR fragment reveals the mechanism for its molecular chaperone activity in UPR."
      Tao J., Petrova K., Ron D., Sha B.
      J. Mol. Biol. 397:1307-1315(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.51 ANGSTROMS) OF 35-393, FUNCTION, MUTAGENESIS OF LEU-48; TYR-71; TYR-75; PHE-104 AND TRP-186.

    Entry informationi

    Entry nameiDNJC3_MOUSE
    AccessioniPrimary (citable) accession number: Q91YW3
    Secondary accession number(s): Q60873
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2005
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3