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Q91YW3

- DNJC3_MOUSE

UniProt

Q91YW3 - DNJC3_MOUSE

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Protein

DnaJ homolog subfamily C member 3

Gene

Dnajc3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.2 Publications

GO - Molecular functioni

  1. chaperone binding Source: BHF-UCL
  2. misfolded protein binding Source: BHF-UCL
  3. protein kinase inhibitor activity Source: MGI

GO - Biological processi

  1. endoplasmic reticulum unfolded protein response Source: BHF-UCL
  2. negative regulation of protein kinase activity Source: MGI
  3. proteolysis involved in cellular protein catabolic process Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Unfolded protein response

Enzyme and pathway databases

ReactomeiREACT_106572. XBP1(S) activates chaperone genes.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 3
Alternative name(s):
Interferon-induced, double-stranded RNA-activated protein kinase inhibitor
Protein kinase inhibitor of 58 kDa
Short name:
Protein kinase inhibitor p58
Gene namesi
Name:Dnajc3
Synonyms:P58ipk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:107373. Dnajc3.

Subcellular locationi

Endoplasmic reticulum 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytosol Source: BHF-UCL
  3. endoplasmic reticulum lumen Source: BHF-UCL
  4. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi48 – 481L → D: Reduces binding affinity for misfolded proteins by 40%. 1 Publication
Mutagenesisi71 – 711Y → A: Reduces binding affinity for misfolded proteins by 40%. 1 Publication
Mutagenesisi75 – 751Y → H: Reduces binding affinity for misfolded proteins by 40%. 1 Publication
Mutagenesisi104 – 1041F → A: Reduces binding affinity for misfolded proteins by 40%. 1 Publication
Mutagenesisi186 – 1861W → A: Doesn't affect binding of misfolded proteins. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 504473DnaJ homolog subfamily C member 3PRO_0000071046Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi248 ↔ 258By similarity
Disulfide bondi313 ↔ 329By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ91YW3.
PaxDbiQ91YW3.
PRIDEiQ91YW3.

2D gel databases

REPRODUCTION-2DPAGEQ91YW3.

PTM databases

PhosphoSiteiQ91YW3.

Expressioni

Tissue specificityi

Widely expressed, with high level in the liver.1 Publication

Inductioni

Up-regulated during an endoplasmic reticulum stress.1 Publication

Gene expression databases

BgeeiQ91YW3.
ExpressionAtlasiQ91YW3. baseline and differential.
GenevestigatoriQ91YW3.

Interactioni

Subunit structurei

Interacts with EIF2AK2. Forms a trimeric complex with DNAJB1 and HSPA8. Interacts with PRKRIR/P52RIPK (By similarity). Interacts with EIF2AK3.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
HSPA5P110212EBI-8381770,EBI-354921From a different organism.

Protein-protein interaction databases

BioGridi781938. 1 interaction.
IntActiQ91YW3. 4 interactions.
MINTiMINT-1838284.

Structurei

Secondary structure

1
504
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi36 – 4914
Helixi53 – 6614
Helixi71 – 8414
Helixi87 – 10014
Helixi105 – 11814
Helixi121 – 13212
Helixi138 – 16629
Helixi170 – 18314
Helixi188 – 20013
Helixi204 – 21512
Helixi222 – 23514
Helixi238 – 25114
Helixi256 – 28025
Helixi284 – 29714
Helixi302 – 31817
Helixi322 – 33514
Helixi340 – 35213
Helixi356 – 36712
Helixi374 – 39118

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3IEGX-ray2.51A/B35-393[»]
ProteinModelPortaliQ91YW3.
SMRiQ91YW3. Positions 35-455.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91YW3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati37 – 7034TPR 1Add
BLAST
Repeati72 – 10433TPR 2Add
BLAST
Repeati105 – 13834TPR 3Add
BLAST
Repeati154 – 18734TPR 4Add
BLAST
Repeati188 – 22134TPR 5Add
BLAST
Repeati222 – 25534TPR 6Add
BLAST
Repeati268 – 30134TPR 7Add
BLAST
Repeati306 – 33934TPR 8Add
BLAST
Repeati340 – 37334TPR 9Add
BLAST
Domaini394 – 46269JPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni375 – 39319Flexible linkerBy similarityAdd
BLAST

Domaini

The J domain mediates interaction with HSPA8.By similarity
Binding to misfolded proteins is mediated by a hydrophobic patch forming a large groove within the first two TPR repeats.

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation
Contains 9 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiCOG0484.
GeneTreeiENSGT00720000108760.
HOGENOMiHOG000193351.
HOVERGENiHBG053820.
InParanoidiQ91YW3.
KOiK09523.
OMAiQSERRDY.
OrthoDBiEOG70KGPH.
PhylomeDBiQ91YW3.
TreeFamiTF105162.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
1.25.40.10. 3 hits.
InterProiIPR001623. DnaJ_domain.
IPR026901. DNAJC3.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERiPTHR24078:SF165. PTHR24078:SF165. 1 hit.
PfamiPF00226. DnaJ. 1 hit.
PF00515. TPR_1. 2 hits.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
SM00028. TPR. 7 hits.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
PS50005. TPR. 8 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91YW3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVAPGSVGSR LGAVFPFLLV LVDLQYEGAE CGVNADVEKH LELGKKLLAA
60 70 80 90 100
GQLADALSQF HAAVDGDPDN YIAYYRRATV FLAMGKSKAA LPDLTKVIAL
110 120 130 140 150
KMDFTAARLQ RGHLLLKQGK LDEAEDDFKK VLKSNPSEQE EKEAESQLVK
160 170 180 190 200
ADEMQRLRSQ ALDAFDGADY TAAITFLDKI LEVCVWDAEL RELRAECFIK
210 220 230 240 250
EGEPRKAISD LKAASKLKSD NTEAFYKIST LYYQLGDHEL SLSEVRECLK
260 270 280 290 300
LDQDHKRCFA HYKQVKKLNK LIESAEELIR DGRYTDATSK YESVMKTEPS
310 320 330 340 350
VAEYTVRSKE RICHCFSKDE KPVEAIRICS EVLQMEPDNV NALKDRAEAY
360 370 380 390 400
LIEEMYDEAI QDYEAAQEHN ENDQQIREGL EKAQRLLKQS QKRDYYKILG
410 420 430 440 450
VKRNAKKQEI IKAYRKLALQ WHPDNFQNEE EKKKAEKKFI DIAAAKEVLS
460 470 480 490 500
DPEMRKKFDD GEDPLDAESQ QGGGGNPFHR SWNSWQGFNP FSSGGPFRFK

FHFN
Length:504
Mass (Da):57,464
Last modified:December 1, 2001 - v1
Checksum:i510C27782C6EDF66
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 913AAL → RRV in AAC52592. (PubMed:8666242)Curated
Sequence conflicti134 – 1341S → C in AAC52592. (PubMed:8666242)Curated
Sequence conflicti405 – 4051A → T in AAC52592. (PubMed:8666242)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U28423 mRNA. Translation: AAC52592.1.
BC013766 mRNA. Translation: AAH13766.1.
CCDSiCCDS37012.1.
RefSeqiNP_032955.2. NM_008929.3.
UniGeneiMm.12616.

Genome annotation databases

EnsembliENSMUST00000022734; ENSMUSP00000022734; ENSMUSG00000022136.
GeneIDi100037258.
KEGGimmu:100037258.
UCSCiuc007uzd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U28423 mRNA. Translation: AAC52592.1 .
BC013766 mRNA. Translation: AAH13766.1 .
CCDSi CCDS37012.1.
RefSeqi NP_032955.2. NM_008929.3.
UniGenei Mm.12616.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3IEG X-ray 2.51 A/B 35-393 [» ]
ProteinModelPortali Q91YW3.
SMRi Q91YW3. Positions 35-455.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 781938. 1 interaction.
IntActi Q91YW3. 4 interactions.
MINTi MINT-1838284.

PTM databases

PhosphoSitei Q91YW3.

2D gel databases

REPRODUCTION-2DPAGE Q91YW3.

Proteomic databases

MaxQBi Q91YW3.
PaxDbi Q91YW3.
PRIDEi Q91YW3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022734 ; ENSMUSP00000022734 ; ENSMUSG00000022136 .
GeneIDi 100037258.
KEGGi mmu:100037258.
UCSCi uc007uzd.2. mouse.

Organism-specific databases

CTDi 5611.
MGIi MGI:107373. Dnajc3.

Phylogenomic databases

eggNOGi COG0484.
GeneTreei ENSGT00720000108760.
HOGENOMi HOG000193351.
HOVERGENi HBG053820.
InParanoidi Q91YW3.
KOi K09523.
OMAi QSERRDY.
OrthoDBi EOG70KGPH.
PhylomeDBi Q91YW3.
TreeFami TF105162.

Enzyme and pathway databases

Reactomei REACT_106572. XBP1(S) activates chaperone genes.

Miscellaneous databases

EvolutionaryTracei Q91YW3.
NextBioi 444137.
PROi Q91YW3.
SOURCEi Search...

Gene expression databases

Bgeei Q91YW3.
ExpressionAtlasi Q91YW3. baseline and differential.
Genevestigatori Q91YW3.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
1.25.40.10. 3 hits.
InterProi IPR001623. DnaJ_domain.
IPR026901. DNAJC3.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view ]
PANTHERi PTHR24078:SF165. PTHR24078:SF165. 1 hit.
Pfami PF00226. DnaJ. 1 hit.
PF00515. TPR_1. 2 hits.
[Graphical view ]
PRINTSi PR00625. JDOMAIN.
SMARTi SM00271. DnaJ. 1 hit.
SM00028. TPR. 7 hits.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
PROSITEi PS50076. DNAJ_2. 1 hit.
PS50005. TPR. 8 hits.
PS50293. TPR_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression, and cellular localization of the oncogenic 58-kDa inhibitor of the RNA-activated human and mouse protein kinase."
    Korth M.J., Lyons C.N., Wambach M., Katze M.G.
    Gene 170:181-188(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor.
  3. "Control of PERK eIF2alpha kinase activity by the endoplasmic reticulum stress-induced molecular chaperone P58IPK."
    Yan W., Frank C.L., Korth M.J., Sopher B.L., Novoa I., Ron D., Katze M.G.
    Proc. Natl. Acad. Sci. U.S.A. 99:15920-15925(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH EIF2AK3, INDUCTION.
  4. "Crystal structure of P58(IPK) TPR fragment reveals the mechanism for its molecular chaperone activity in UPR."
    Tao J., Petrova K., Ron D., Sha B.
    J. Mol. Biol. 397:1307-1315(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.51 ANGSTROMS) OF 35-393, FUNCTION, MUTAGENESIS OF LEU-48; TYR-71; TYR-75; PHE-104 AND TRP-186.

Entry informationi

Entry nameiDNJC3_MOUSE
AccessioniPrimary (citable) accession number: Q91YW3
Secondary accession number(s): Q60873
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: December 1, 2001
Last modified: October 29, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3