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Protein

DnaJ homolog subfamily C member 3

Gene

Dnajc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions (PubMed:25329545). Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity (By similarity).By similarity3 Publications

GO - Molecular functioni

  • chaperone binding Source: BHF-UCL
  • misfolded protein binding Source: BHF-UCL
  • protein kinase binding Source: UniProtKB
  • protein kinase inhibitor activity Source: UniProtKB

GO - Biological processi

  • cellular response to cold Source: UniProtKB
  • endoplasmic reticulum unfolded protein response Source: BHF-UCL
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation Source: UniProtKB
  • negative regulation of protein kinase activity Source: MGI
  • positive regulation of translation initiation in response to endoplasmic reticulum stress Source: UniProtKB
  • proteolysis involved in cellular protein catabolic process Source: BHF-UCL

Keywordsi

Molecular functionChaperone, Repressor
Biological processStress response, Translation regulation, Unfolded protein response

Enzyme and pathway databases

ReactomeiR-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
R-MMU-6798695. Neutrophil degranulation.
R-MMU-8957275. Post-translational protein phosphorylation.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily C member 3
Alternative name(s):
Interferon-induced, double-stranded RNA-activated protein kinase inhibitor
Protein kinase inhibitor of 58 kDa
Short name:
Protein kinase inhibitor p58
Gene namesi
Name:Dnajc3
Synonyms:P58ipk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:107373. Dnajc3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum lumen Source: BHF-UCL
  • extracellular exosome Source: MGI
  • extracellular vesicle Source: MGI
  • membrane Source: MGI
  • smooth endoplasmic reticulum Source: Ensembl

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi48L → D: Reduces binding affinity for misfolded proteins by 40%. 1 Publication1
Mutagenesisi71Y → A: Reduces binding affinity for misfolded proteins by 40%. 1 Publication1
Mutagenesisi75Y → H: Reduces binding affinity for misfolded proteins by 40%. 1 Publication1
Mutagenesisi104F → A: Reduces binding affinity for misfolded proteins by 40%. 1 Publication1
Mutagenesisi186W → A: Doesn't affect binding of misfolded proteins. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Sequence analysisAdd BLAST31
ChainiPRO_000007104632 – 504DnaJ homolog subfamily C member 3Add BLAST473

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi248 ↔ 258By similarity
Modified residuei274PhosphoserineBy similarity1
Disulfide bondi313 ↔ 329By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiQ91YW3.
MaxQBiQ91YW3.
PaxDbiQ91YW3.
PeptideAtlasiQ91YW3.
PRIDEiQ91YW3.

2D gel databases

REPRODUCTION-2DPAGEiQ91YW3.

PTM databases

iPTMnetiQ91YW3.
PhosphoSitePlusiQ91YW3.

Expressioni

Tissue specificityi

Widely expressed, with high level in the liver.1 Publication

Inductioni

Up-regulated during an endoplasmic reticulum stress.1 Publication

Gene expression databases

BgeeiENSMUSG00000022136.
ExpressionAtlasiQ91YW3. baseline and differential.
GenevisibleiQ91YW3. MM.

Interactioni

Subunit structurei

Interacts with EIF2AK4/GCN2; this interaction occurs under endoplasmic reticulum (ER) stress, hypothermic and amino acid starving stress conditions and inhibits EIF2AK4/GCN2 kinase activity (PubMed:25329545). Interacts with EIF2AK3 (PubMed:12446838). Interacts with EIF2AK2. Forms a trimeric complex with DNAJB1 and HSPA8. Interacts with THAP12 (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HSPA5P110212EBI-8381770,EBI-354921From Homo sapiens.

GO - Molecular functioni

  • chaperone binding Source: BHF-UCL
  • misfolded protein binding Source: BHF-UCL
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi781938. 1 interactor.
IntActiQ91YW3. 4 interactors.
MINTiMINT-1838284.
STRINGi10090.ENSMUSP00000022734.

Structurei

Secondary structure

1504
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi36 – 49Combined sources14
Helixi53 – 66Combined sources14
Helixi71 – 84Combined sources14
Helixi87 – 100Combined sources14
Helixi105 – 118Combined sources14
Helixi121 – 132Combined sources12
Helixi138 – 166Combined sources29
Helixi170 – 183Combined sources14
Helixi188 – 200Combined sources13
Helixi204 – 215Combined sources12
Helixi222 – 235Combined sources14
Helixi238 – 251Combined sources14
Helixi256 – 280Combined sources25
Helixi284 – 297Combined sources14
Helixi302 – 318Combined sources17
Helixi322 – 335Combined sources14
Helixi340 – 352Combined sources13
Helixi356 – 367Combined sources12
Helixi374 – 391Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3IEGX-ray2.51A/B35-393[»]
ProteinModelPortaliQ91YW3.
SMRiQ91YW3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91YW3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 70TPR 1Add BLAST34
Repeati72 – 104TPR 2Add BLAST33
Repeati105 – 138TPR 3Add BLAST34
Repeati154 – 187TPR 4Add BLAST34
Repeati188 – 221TPR 5Add BLAST34
Repeati222 – 255TPR 6Add BLAST34
Repeati268 – 301TPR 7Add BLAST34
Repeati306 – 339TPR 8Add BLAST34
Repeati340 – 373TPR 9Add BLAST34
Domaini394 – 462JPROSITE-ProRule annotationAdd BLAST69

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni375 – 393Flexible linkerBy similarityAdd BLAST19

Domaini

The J domain mediates interaction with HSPA8.By similarity
Binding to misfolded proteins is mediated by a hydrophobic patch forming a large groove within the first two TPR repeats.

Keywords - Domaini

Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiKOG0624. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00890000139418.
HOGENOMiHOG000193351.
HOVERGENiHBG053820.
InParanoidiQ91YW3.
KOiK09523.
OMAiGAECGVN.
OrthoDBiEOG091G0783.
PhylomeDBiQ91YW3.
TreeFamiTF105162.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
1.25.40.10. 4 hits.
InterProiView protein in InterPro
IPR001623. DnaJ_domain.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR018704. TPR_21.
IPR019734. TPR_repeat.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF09976. TPR_21. 1 hit.
PF13181. TPR_8. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SM00028. TPR. 7 hits.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF48452. SSF48452. 3 hits.
PROSITEiView protein in PROSITE
PS50076. DNAJ_2. 1 hit.
PS50005. TPR. 8 hits.
PS50293. TPR_REGION. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91YW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAPGSVGSR LGAVFPFLLV LVDLQYEGAE CGVNADVEKH LELGKKLLAA
60 70 80 90 100
GQLADALSQF HAAVDGDPDN YIAYYRRATV FLAMGKSKAA LPDLTKVIAL
110 120 130 140 150
KMDFTAARLQ RGHLLLKQGK LDEAEDDFKK VLKSNPSEQE EKEAESQLVK
160 170 180 190 200
ADEMQRLRSQ ALDAFDGADY TAAITFLDKI LEVCVWDAEL RELRAECFIK
210 220 230 240 250
EGEPRKAISD LKAASKLKSD NTEAFYKIST LYYQLGDHEL SLSEVRECLK
260 270 280 290 300
LDQDHKRCFA HYKQVKKLNK LIESAEELIR DGRYTDATSK YESVMKTEPS
310 320 330 340 350
VAEYTVRSKE RICHCFSKDE KPVEAIRICS EVLQMEPDNV NALKDRAEAY
360 370 380 390 400
LIEEMYDEAI QDYEAAQEHN ENDQQIREGL EKAQRLLKQS QKRDYYKILG
410 420 430 440 450
VKRNAKKQEI IKAYRKLALQ WHPDNFQNEE EKKKAEKKFI DIAAAKEVLS
460 470 480 490 500
DPEMRKKFDD GEDPLDAESQ QGGGGNPFHR SWNSWQGFNP FSSGGPFRFK

FHFN
Length:504
Mass (Da):57,464
Last modified:December 1, 2001 - v1
Checksum:i510C27782C6EDF66
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89 – 91AAL → RRV in AAC52592 (PubMed:8666242).Curated3
Sequence conflicti134S → C in AAC52592 (PubMed:8666242).Curated1
Sequence conflicti405A → T in AAC52592 (PubMed:8666242).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28423 mRNA. Translation: AAC52592.1.
BC013766 mRNA. Translation: AAH13766.1.
CCDSiCCDS37012.1.
RefSeqiNP_032955.2. NM_008929.3.
UniGeneiMm.12616.

Genome annotation databases

EnsembliENSMUST00000022734; ENSMUSP00000022734; ENSMUSG00000022136.
GeneIDi100037258.
KEGGimmu:100037258.
UCSCiuc007uzd.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiDNJC3_MOUSE
AccessioniPrimary (citable) accession number: Q91YW3
Secondary accession number(s): Q60873
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: December 1, 2001
Last modified: August 30, 2017
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references