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Protein

Pre-mRNA-processing factor 6

Gene

Prpf6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163 mRNA Splicing - Major Pathway
R-MMU-72165 mRNA Splicing - Minor Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing factor 6
Alternative name(s):
PRP6 homolog
U5 snRNP-associated 102 kDa protein
Short name:
U5-102 kDa protein
Gene namesi
Name:Prpf6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1922946 Prpf6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002057601 – 941Pre-mRNA-processing factor 6Add BLAST941

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei143PhosphoserineBy similarity1
Modified residuei180PhosphothreonineBy similarity1
Modified residuei266PhosphothreonineBy similarity1
Modified residuei275PhosphothreonineCombined sources1
Modified residuei279PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ91YR7
PaxDbiQ91YR7
PeptideAtlasiQ91YR7
PRIDEiQ91YR7

PTM databases

iPTMnetiQ91YR7
PhosphoSitePlusiQ91YR7

Expressioni

Gene expression databases

BgeeiENSMUSG00000002455
CleanExiMM_PRPF6
ExpressionAtlasiQ91YR7 baseline and differential
GenevisibleiQ91YR7 MM

Interactioni

Subunit structurei

Associates with the U5 snRNP particle. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, LSm proteins LSm2-8 and Sm proteins. Interacts with ARAF1. Identified in the spliceosome C complex (By similarity). Interacts with AR and NR3C1, but not ESR1, independently of the presence of hormones (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213097, 4 interactors
IntActiQ91YR7, 4 interactors
MINTiQ91YR7
STRINGi10090.ENSMUSP00000002529

Structurei

3D structure databases

ProteinModelPortaliQ91YR7
SMRiQ91YR7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati384 – 416HAT 1Add BLAST33
Repeati418 – 444HAT 2Add BLAST27
Repeati445 – 476HAT 3Add BLAST32
Repeati554 – 586HAT 4Add BLAST33
Repeati588 – 620HAT 5Add BLAST33
Repeati622 – 654HAT 6Add BLAST33
Repeati689 – 721HAT 7Add BLAST33
Repeati723 – 755HAT 8Add BLAST33
Repeati855 – 887HAT 9Add BLAST33

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi67 – 72Poly-Asp6

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0495 Eukaryota
ENOG410XRKD LUCA
GeneTreeiENSGT00550000075016
HOGENOMiHOG000116247
HOVERGENiHBG023330
InParanoidiQ91YR7
KOiK12855
OMAiMKSARLE
OrthoDBiEOG091G01F5
PhylomeDBiQ91YR7
TreeFamiTF105743

Family and domain databases

Gene3Di1.25.40.10, 5 hits
InterProiView protein in InterPro
IPR003107 HAT
IPR010491 PRP1_N
IPR027108 Prp6/Prp1/STA1
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
PANTHERiPTHR11246:SF1 PTHR11246:SF1, 1 hit
PfamiView protein in Pfam
PF06424 PRP1_N, 1 hit
SMARTiView protein in SMART
SM00386 HAT, 13 hits
SM00028 TPR, 4 hits
SUPFAMiSSF48452 SSF48452, 4 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91YR7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNKKKKPFLG MPAPLGYVPG LGRGATGFTT RSDIGPARDA NDPVDDRHAP
60 70 80 90 100
PGKRTVGDQM KKNQAADDDD EDLNDTNYDE FNGYAGSLFS SGPYEKDDEE
110 120 130 140 150
ADAIYAALDK RMDERRKERR EQREKEEIEK YRMERPKIQQ QFSDLKRKLA
160 170 180 190 200
EVTEEEWLSI PEVGDARNKR QRNPRYEKLT PVPDSFFAKH LQTGENHTSV
210 220 230 240 250
DPRQTQFGGL NTPYPGGLNT PYPGGMTPGL MTPGTGELDM RKIGQARNTL
260 270 280 290 300
MDMRLSQVSD SVSGQTVVDP KGYLTDLNSM IPTHGGDIND IKKARLLLKS
310 320 330 340 350
VRETNPHHPP AWIASARLEE VTGKLQVARN LIMKGTEMCP KSEDVWLEAA
360 370 380 390 400
RLQPGDTAKA VVAQAVRHLP QSVRIYIRAA ELETDIRAKK RVLRKALEHV
410 420 430 440 450
PNSVRLWKAA VELEEPEDAR IMLSRAVECC PTSVELWLAL ARLETYENAR
460 470 480 490 500
KVLNKARENI PTDRHIWITA AKLEEANGNT QMVEKIIDRA ITSLRANGVE
510 520 530 540 550
INREQWIQDA EECDRAGSVA TCQAVMRAVI GIGIEEEDRK HTWMEDADSC
560 570 580 590 600
VAHNALECAR AIYAYALQVF PSKKSVWLRA AYFEKNHGTR ESLEALLQRA
610 620 630 640 650
VAHCPKAEVL WLMGAKSKWL AGDVPAARSI LALAFQANPN SEEIWLAAVK
660 670 680 690 700
LESENNEYER ARRLLAKARS SAPTARVFMK SVKLEWVLGN ISAAQELCEE
710 720 730 740 750
ALRHYEDFPK LWMMKGQIEE QGELMEKARE AYNQGLKKCP HSTPLWLLLS
760 770 780 790 800
RLEEKIGQLT RARAILEKSR LKNPKNPGLW LESVRLEYRA GLKNIANTLM
810 820 830 840 850
AKALQECPNS GILWSEAVFL EARPQRKTKS VDALKKCEHD PHVLLAVAKL
860 870 880 890 900
FWSERKITKA REWFHRTVKI DSDLGDAWAF FYKFELQHGT EEQQEEVRKR
910 920 930 940
CENAEPRHGE LWCAVSKDIT NWQRKIGEIL VLVAARIKNT F
Length:941
Mass (Da):106,722
Last modified:December 1, 2001 - v1
Checksum:iA08C3AC49AE1B9E9
GO
Isoform 2 (identifier: Q91YR7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     550-570: CVAHNALECARAIYAYALQVF → VSFLACFPACSLDRNSGPINL
     571-941: Missing.

Note: No experimental confirmation available.
Show »
Length:570
Mass (Da):63,960
Checksum:i16E5B26A91A0C60B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232T → A in BAE26451 (PubMed:16141072).Curated1
Sequence conflicti390K → R in BAE26451 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002063550 – 570CVAHN…ALQVF → VSFLACFPACSLDRNSGPIN L in isoform 2. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_002064571 – 941Missing in isoform 2. 1 PublicationAdd BLAST371

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC005801 mRNA Translation: AAH05801.1
BC014869 mRNA Translation: AAH14869.1
BC023691 mRNA Translation: AAH23691.2
BC025030 mRNA Translation: AAH25030.1
AK011639 mRNA Translation: BAB27751.1
AK145461 mRNA Translation: BAE26451.1
AK081998 mRNA Translation: BAC38390.1
AL844529 Genomic DNA Translation: CAX15563.1
CH466626 Genomic DNA Translation: EDL07438.1
CCDSiCCDS38381.1 [Q91YR7-1]
RefSeqiNP_598462.1, NM_133701.2 [Q91YR7-1]
UniGeneiMm.292001

Genome annotation databases

EnsembliENSMUST00000002529; ENSMUSP00000002529; ENSMUSG00000002455 [Q91YR7-1]
ENSMUST00000136481; ENSMUSP00000121340; ENSMUSG00000002455 [Q91YR7-1]
GeneIDi68879
KEGGimmu:68879
UCSCiuc008omy.1 mouse [Q91YR7-2]
uc008omz.1 mouse [Q91YR7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPRP6_MOUSE
AccessioniPrimary (citable) accession number: Q91YR7
Secondary accession number(s): Q3ULJ7
, Q542P0, Q8CIK9, Q8R3M8, Q99JN1, Q9CSZ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: December 1, 2001
Last modified: May 23, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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