Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pre-mRNA-processing factor 6

Gene

Prpf6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-processing factor 6
Alternative name(s):
PRP6 homolog
U5 snRNP-associated 102 kDa protein
Short name:
U5-102 kDa protein
Gene namesi
Name:Prpf6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1922946. Prpf6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002057601 – 941Pre-mRNA-processing factor 6Add BLAST941

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei143PhosphoserineBy similarity1
Modified residuei180PhosphothreonineBy similarity1
Modified residuei266PhosphothreonineBy similarity1
Modified residuei275PhosphothreonineCombined sources1
Modified residuei279PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ91YR7.
PaxDbiQ91YR7.
PeptideAtlasiQ91YR7.
PRIDEiQ91YR7.

PTM databases

iPTMnetiQ91YR7.
PhosphoSitePlusiQ91YR7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000002455.
CleanExiMM_PRPF6.
ExpressionAtlasiQ91YR7. baseline and differential.
GenevisibleiQ91YR7. MM.

Interactioni

Subunit structurei

Associates with the U5 snRNP particle. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39, LSm proteins LSm2-8 and Sm proteins. Interacts with ARAF1. Identified in the spliceosome C complex (By similarity). Interacts with AR and NR3C1, but not ESR1, independently of the presence of hormones (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213097. 3 interactors.
IntActiQ91YR7. 3 interactors.
MINTiMINT-4108694.
STRINGi10090.ENSMUSP00000002529.

Structurei

3D structure databases

ProteinModelPortaliQ91YR7.
SMRiQ91YR7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati384 – 416HAT 1Add BLAST33
Repeati418 – 444HAT 2Add BLAST27
Repeati445 – 476HAT 3Add BLAST32
Repeati554 – 586HAT 4Add BLAST33
Repeati588 – 620HAT 5Add BLAST33
Repeati622 – 654HAT 6Add BLAST33
Repeati689 – 721HAT 7Add BLAST33
Repeati723 – 755HAT 8Add BLAST33
Repeati855 – 887HAT 9Add BLAST33

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi67 – 72Poly-Asp6

Sequence similaritiesi

Contains 9 HAT repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0495. Eukaryota.
ENOG410XRKD. LUCA.
GeneTreeiENSGT00550000075016.
HOGENOMiHOG000116247.
HOVERGENiHBG023330.
InParanoidiQ91YR7.
KOiK12855.
OMAiRQRFYAV.
OrthoDBiEOG091G01F5.
PhylomeDBiQ91YR7.
TreeFamiTF105743.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
InterProiIPR003107. HAT.
IPR010491. PRP1_N.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF06424. PRP1_N. 1 hit.
[Graphical view]
SMARTiSM00386. HAT. 13 hits.
SM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 4 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91YR7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNKKKKPFLG MPAPLGYVPG LGRGATGFTT RSDIGPARDA NDPVDDRHAP
60 70 80 90 100
PGKRTVGDQM KKNQAADDDD EDLNDTNYDE FNGYAGSLFS SGPYEKDDEE
110 120 130 140 150
ADAIYAALDK RMDERRKERR EQREKEEIEK YRMERPKIQQ QFSDLKRKLA
160 170 180 190 200
EVTEEEWLSI PEVGDARNKR QRNPRYEKLT PVPDSFFAKH LQTGENHTSV
210 220 230 240 250
DPRQTQFGGL NTPYPGGLNT PYPGGMTPGL MTPGTGELDM RKIGQARNTL
260 270 280 290 300
MDMRLSQVSD SVSGQTVVDP KGYLTDLNSM IPTHGGDIND IKKARLLLKS
310 320 330 340 350
VRETNPHHPP AWIASARLEE VTGKLQVARN LIMKGTEMCP KSEDVWLEAA
360 370 380 390 400
RLQPGDTAKA VVAQAVRHLP QSVRIYIRAA ELETDIRAKK RVLRKALEHV
410 420 430 440 450
PNSVRLWKAA VELEEPEDAR IMLSRAVECC PTSVELWLAL ARLETYENAR
460 470 480 490 500
KVLNKARENI PTDRHIWITA AKLEEANGNT QMVEKIIDRA ITSLRANGVE
510 520 530 540 550
INREQWIQDA EECDRAGSVA TCQAVMRAVI GIGIEEEDRK HTWMEDADSC
560 570 580 590 600
VAHNALECAR AIYAYALQVF PSKKSVWLRA AYFEKNHGTR ESLEALLQRA
610 620 630 640 650
VAHCPKAEVL WLMGAKSKWL AGDVPAARSI LALAFQANPN SEEIWLAAVK
660 670 680 690 700
LESENNEYER ARRLLAKARS SAPTARVFMK SVKLEWVLGN ISAAQELCEE
710 720 730 740 750
ALRHYEDFPK LWMMKGQIEE QGELMEKARE AYNQGLKKCP HSTPLWLLLS
760 770 780 790 800
RLEEKIGQLT RARAILEKSR LKNPKNPGLW LESVRLEYRA GLKNIANTLM
810 820 830 840 850
AKALQECPNS GILWSEAVFL EARPQRKTKS VDALKKCEHD PHVLLAVAKL
860 870 880 890 900
FWSERKITKA REWFHRTVKI DSDLGDAWAF FYKFELQHGT EEQQEEVRKR
910 920 930 940
CENAEPRHGE LWCAVSKDIT NWQRKIGEIL VLVAARIKNT F
Length:941
Mass (Da):106,722
Last modified:December 1, 2001 - v1
Checksum:iA08C3AC49AE1B9E9
GO
Isoform 2 (identifier: Q91YR7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     550-570: CVAHNALECARAIYAYALQVF → VSFLACFPACSLDRNSGPINL
     571-941: Missing.

Note: No experimental confirmation available.
Show »
Length:570
Mass (Da):63,960
Checksum:i16E5B26A91A0C60B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti232T → A in BAE26451 (PubMed:16141072).Curated1
Sequence conflicti390K → R in BAE26451 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002063550 – 570CVAHN…ALQVF → VSFLACFPACSLDRNSGPIN L in isoform 2. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_002064571 – 941Missing in isoform 2. 1 PublicationAdd BLAST371

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC005801 mRNA. Translation: AAH05801.1.
BC014869 mRNA. Translation: AAH14869.1.
BC023691 mRNA. Translation: AAH23691.2.
BC025030 mRNA. Translation: AAH25030.1.
AK011639 mRNA. Translation: BAB27751.1.
AK145461 mRNA. Translation: BAE26451.1.
AK081998 mRNA. Translation: BAC38390.1.
AL844529 Genomic DNA. Translation: CAX15563.1.
CH466626 Genomic DNA. Translation: EDL07438.1.
CCDSiCCDS38381.1. [Q91YR7-1]
RefSeqiNP_598462.1. NM_133701.2. [Q91YR7-1]
UniGeneiMm.292001.

Genome annotation databases

EnsembliENSMUST00000002529; ENSMUSP00000002529; ENSMUSG00000002455. [Q91YR7-1]
ENSMUST00000136481; ENSMUSP00000121340; ENSMUSG00000002455. [Q91YR7-1]
GeneIDi68879.
KEGGimmu:68879.
UCSCiuc008omy.1. mouse. [Q91YR7-2]
uc008omz.1. mouse. [Q91YR7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC005801 mRNA. Translation: AAH05801.1.
BC014869 mRNA. Translation: AAH14869.1.
BC023691 mRNA. Translation: AAH23691.2.
BC025030 mRNA. Translation: AAH25030.1.
AK011639 mRNA. Translation: BAB27751.1.
AK145461 mRNA. Translation: BAE26451.1.
AK081998 mRNA. Translation: BAC38390.1.
AL844529 Genomic DNA. Translation: CAX15563.1.
CH466626 Genomic DNA. Translation: EDL07438.1.
CCDSiCCDS38381.1. [Q91YR7-1]
RefSeqiNP_598462.1. NM_133701.2. [Q91YR7-1]
UniGeneiMm.292001.

3D structure databases

ProteinModelPortaliQ91YR7.
SMRiQ91YR7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213097. 3 interactors.
IntActiQ91YR7. 3 interactors.
MINTiMINT-4108694.
STRINGi10090.ENSMUSP00000002529.

PTM databases

iPTMnetiQ91YR7.
PhosphoSitePlusiQ91YR7.

Proteomic databases

EPDiQ91YR7.
PaxDbiQ91YR7.
PeptideAtlasiQ91YR7.
PRIDEiQ91YR7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002529; ENSMUSP00000002529; ENSMUSG00000002455. [Q91YR7-1]
ENSMUST00000136481; ENSMUSP00000121340; ENSMUSG00000002455. [Q91YR7-1]
GeneIDi68879.
KEGGimmu:68879.
UCSCiuc008omy.1. mouse. [Q91YR7-2]
uc008omz.1. mouse. [Q91YR7-1]

Organism-specific databases

CTDi24148.
MGIiMGI:1922946. Prpf6.

Phylogenomic databases

eggNOGiKOG0495. Eukaryota.
ENOG410XRKD. LUCA.
GeneTreeiENSGT00550000075016.
HOGENOMiHOG000116247.
HOVERGENiHBG023330.
InParanoidiQ91YR7.
KOiK12855.
OMAiRQRFYAV.
OrthoDBiEOG091G01F5.
PhylomeDBiQ91YR7.
TreeFamiTF105743.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72165. mRNA Splicing - Minor Pathway.

Miscellaneous databases

PROiQ91YR7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002455.
CleanExiMM_PRPF6.
ExpressionAtlasiQ91YR7. baseline and differential.
GenevisibleiQ91YR7. MM.

Family and domain databases

Gene3Di1.25.40.10. 4 hits.
InterProiIPR003107. HAT.
IPR010491. PRP1_N.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF06424. PRP1_N. 1 hit.
[Graphical view]
SMARTiSM00386. HAT. 13 hits.
SM00028. TPR. 4 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 4 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPRP6_MOUSE
AccessioniPrimary (citable) accession number: Q91YR7
Secondary accession number(s): Q3ULJ7
, Q542P0, Q8CIK9, Q8R3M8, Q99JN1, Q9CSZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.