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Reviewed, UniProtKB/Swiss-Prot Q91YQ5 (RPN1_MOUSE)

Last modified November 3, 2009. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
    EC=2.4.1.119
Alternative name(s):
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
    Ribophorin-1
    Ribophorin I
      Short name=RPN-I
Gene names
Name: Rpn1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length608 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.

Catalytic activity

Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.

Subunit structure

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Melanosome By similarity.

Sequence similarities

Belongs to the OST1 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 By similarity
Chain26 – 608583Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1
PRO_0000022242

Regions

Topological domain26 – 440415Lumenal Potential
Transmembrane441 – 45818 Potential
Topological domain459 – 608150Cytoplasmic Potential

Amino acid modifications

Modified residue1881N6-acetyllysine By similarity
Glycosylation3001N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
Q91YQ5-1 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: CBDFA74C792A496E

FASTA60868,528
        10         20         30         40         50         60 
MESPVALLLL LLLCLGALAP TPGSASSEAP PLVNEDVKRT VDLSSHLAKV TAEVVLVHPG 

        70         80         90        100        110        120 
GGSTSRASSF VLALEPELES RLAHLGVQIK GEDEEDNNLE VRETKIKGKS GRFFTVKLPV 

       130        140        150        160        170        180 
ALDPGSKISV VVETVYTHVL HPYPTQITQS EKQFVVFEGN HYFYSPYPTK TQTMRVKLAS 

       190        200        210        220        230        240 
RNVESYTKLG NPSRSEDVLD YGPFKDIPAY SQDTFKVHYE NNSPFLTITS MTRVIEVSHW 

       250        260        270        280        290        300 
GNIAVEENVD LKHTGAVLKG PFSRYDYQRQ PDSGISSIRS FKTILPAAAQ DVYYRDEIGN 

       310        320        330        340        350        360 
VSTSHLLILD DSVEMEIRPR FPLFGGWKTH YIVGYNLPSY EYLYNLGDQY ALKMRFVDHV 

       370        380        390        400        410        420 
FDEQVIDSLT VKIILPEGAK NIQVDSPYDI SRAPDELHYT YLDTFGRPVI VAYKKNLVEQ 

       430        440        450        460        470        480 
HIQDIVVHYT FNKVLMLQEP LLVVAAFYIL FFTVIIYVRL DFSITKDPAA EARMKVACIT 

       490        500        510        520        530        540 
EQVLTLVNKR LGLYRHFDET VNRYKQSRDI STLNSGKKSL ETEHKAVTSE IAVLQSRLKT 

       550        560        570        580        590        600 
EGSDLCDRVS EMQKLDAQVK ELVLKSAVEA ERLVAGKLKK DTYLENEKLS SGKRQELVTK 


IDHILDAL

« Hide

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References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Bone marrow, Eye, Testis and Thymus.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

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Cross-references

Sequence databases

AK052130 mRNA. Translation: BAC34853.1.
AK132750 mRNA. Translation: BAE21332.1.
AK151899 mRNA. Translation: BAE30782.1.
AK153867 mRNA. Translation: BAE32221.1.
BC016080 mRNA. Translation: AAH16080.1.
IPIIPI00309035.
UniGeneMm.188544

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActQ91YQ5. 2 interactions.
STRINGQ91YQ5.

PTM databases

PhosphoSiteQ91YQ5.

Proteomic databases

PRIDEQ91YQ5.

Genome annotation databases

EnsemblENSMUST00000032143; ENSMUSP00000032143; ENSMUSG00000030062; Mus musculus. [Genome view]

Organism-specific databases

MGIMGI:98084. Rpn1.

Phylogenomic databases

HOGENOMQ91YQ5.
HOVERGENQ91YQ5.
OMAENNLEVR.

Enzyme and pathway databases

BRENDA2.4.1.119. 244.

Gene expression databases

ArrayExpressQ91YQ5.
BgeeQ91YQ5.
CleanExMM_RPN1.
GenevestigatorQ91YQ5.
GermOnlineENSMUSG00000030062. Mus musculus.

Family and domain databases

InterProIPR007676. Ribophorin_I.
[Graphical view]
PANTHERPTHR21049. Ribophorin_I. 1 hit.
PfamPF04597. Ribophorin_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry nameRPN1_MOUSE
AccessionPrimary (citable) accession number: Q91YQ5
Secondary accession number(s): Q3U985
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: December 1, 2001
Last modified: November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents