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Q91YQ5

- RPN1_MOUSE

UniProt

Q91YQ5 - RPN1_MOUSE

Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

Gene

Rpn1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarity

    Catalytic activityi

    Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

    Pathwayi

    GO - Molecular functioni

    1. dolichyl-diphosphooligosaccharide-protein glycotransferase activity Source: InterPro

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    ReactomeiREACT_196445. SRP-dependent cotranslational protein targeting to membrane.
    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (EC:2.4.99.18)
    Alternative name(s):
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
    Ribophorin I
    Short name:
    RPN-I
    Ribophorin-1
    Gene namesi
    Name:Rpn1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:98084. Rpn1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: MGI
    2. integral component of membrane Source: UniProtKB-KW
    3. melanosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525By similarityAdd
    BLAST
    Chaini26 – 608583Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1PRO_0000022242Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei188 – 1881N6-acetyllysineBy similarity
    Glycosylationi300 – 3001N-linked (GlcNAc...)Sequence Analysis
    Modified residuei539 – 5391N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Glycoprotein

    Proteomic databases

    MaxQBiQ91YQ5.
    PaxDbiQ91YQ5.
    PRIDEiQ91YQ5.

    PTM databases

    PhosphoSiteiQ91YQ5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ91YQ5.
    BgeeiQ91YQ5.
    CleanExiMM_RPN1.
    GenevestigatoriQ91YQ5.

    Interactioni

    Subunit structurei

    Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex By similarity.By similarity

    Protein-protein interaction databases

    BioGridi222240. 2 interactions.
    IntActiQ91YQ5. 3 interactions.
    MINTiMINT-1581523.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91YQ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini26 – 440415LumenalSequence AnalysisAdd
    BLAST
    Topological domaini459 – 608150CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei441 – 45818HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the OST1 family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG292474.
    GeneTreeiENSGT00390000009630.
    HOGENOMiHOG000247000.
    HOVERGENiHBG012864.
    InParanoidiQ91YQ5.
    KOiK12666.
    OMAiTFKVHYE.
    OrthoDBiEOG7GFB53.
    PhylomeDBiQ91YQ5.
    TreeFamiTF312988.

    Family and domain databases

    InterProiIPR007676. Ribophorin_I.
    [Graphical view]
    PANTHERiPTHR21049. PTHR21049. 1 hit.
    PfamiPF04597. Ribophorin_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q91YQ5-1 [UniParc]FASTAAdd to Basket

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    MESPVALLLL LLLCLGALAP TPGSASSEAP PLVNEDVKRT VDLSSHLAKV    50
    TAEVVLVHPG GGSTSRASSF VLALEPELES RLAHLGVQIK GEDEEDNNLE 100
    VRETKIKGKS GRFFTVKLPV ALDPGSKISV VVETVYTHVL HPYPTQITQS 150
    EKQFVVFEGN HYFYSPYPTK TQTMRVKLAS RNVESYTKLG NPSRSEDVLD 200
    YGPFKDIPAY SQDTFKVHYE NNSPFLTITS MTRVIEVSHW GNIAVEENVD 250
    LKHTGAVLKG PFSRYDYQRQ PDSGISSIRS FKTILPAAAQ DVYYRDEIGN 300
    VSTSHLLILD DSVEMEIRPR FPLFGGWKTH YIVGYNLPSY EYLYNLGDQY 350
    ALKMRFVDHV FDEQVIDSLT VKIILPEGAK NIQVDSPYDI SRAPDELHYT 400
    YLDTFGRPVI VAYKKNLVEQ HIQDIVVHYT FNKVLMLQEP LLVVAAFYIL 450
    FFTVIIYVRL DFSITKDPAA EARMKVACIT EQVLTLVNKR LGLYRHFDET 500
    VNRYKQSRDI STLNSGKKSL ETEHKAVTSE IAVLQSRLKT EGSDLCDRVS 550
    EMQKLDAQVK ELVLKSAVEA ERLVAGKLKK DTYLENEKLS SGKRQELVTK 600
    IDHILDAL 608
    Length:608
    Mass (Da):68,528
    Last modified:December 1, 2001 - v1
    Checksum:iCBDFA74C792A496E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK052130 mRNA. Translation: BAC34853.1.
    AK132750 mRNA. Translation: BAE21332.1.
    AK151899 mRNA. Translation: BAE30782.1.
    AK153867 mRNA. Translation: BAE32221.1.
    BC016080 mRNA. Translation: AAH16080.1.
    CCDSiCCDS20332.1.
    RefSeqiNP_598694.3. NM_133933.4.
    UniGeneiMm.188544.

    Genome annotation databases

    EnsembliENSMUST00000032143; ENSMUSP00000032143; ENSMUSG00000030062.
    GeneIDi103963.
    KEGGimmu:103963.
    UCSCiuc009cva.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK052130 mRNA. Translation: BAC34853.1 .
    AK132750 mRNA. Translation: BAE21332.1 .
    AK151899 mRNA. Translation: BAE30782.1 .
    AK153867 mRNA. Translation: BAE32221.1 .
    BC016080 mRNA. Translation: AAH16080.1 .
    CCDSi CCDS20332.1.
    RefSeqi NP_598694.3. NM_133933.4.
    UniGenei Mm.188544.

    3D structure databases

    ProteinModelPortali Q91YQ5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 222240. 2 interactions.
    IntActi Q91YQ5. 3 interactions.
    MINTi MINT-1581523.

    PTM databases

    PhosphoSitei Q91YQ5.

    Proteomic databases

    MaxQBi Q91YQ5.
    PaxDbi Q91YQ5.
    PRIDEi Q91YQ5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032143 ; ENSMUSP00000032143 ; ENSMUSG00000030062 .
    GeneIDi 103963.
    KEGGi mmu:103963.
    UCSCi uc009cva.2. mouse.

    Organism-specific databases

    CTDi 6184.
    MGIi MGI:98084. Rpn1.

    Phylogenomic databases

    eggNOGi NOG292474.
    GeneTreei ENSGT00390000009630.
    HOGENOMi HOG000247000.
    HOVERGENi HBG012864.
    InParanoidi Q91YQ5.
    KOi K12666.
    OMAi TFKVHYE.
    OrthoDBi EOG7GFB53.
    PhylomeDBi Q91YQ5.
    TreeFami TF312988.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_196445. SRP-dependent cotranslational protein targeting to membrane.

    Miscellaneous databases

    ChiTaRSi RPN1. mouse.
    NextBioi 356253.
    PROi Q91YQ5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q91YQ5.
    Bgeei Q91YQ5.
    CleanExi MM_RPN1.
    Genevestigatori Q91YQ5.

    Family and domain databases

    InterProi IPR007676. Ribophorin_I.
    [Graphical view ]
    PANTHERi PTHR21049. PTHR21049. 1 hit.
    Pfami PF04597. Ribophorin_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Bone marrow, Eye, Testis and Thymus.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-539, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiRPN1_MOUSE
    AccessioniPrimary (citable) accession number: Q91YQ5
    Secondary accession number(s): Q3U985
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2004
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3