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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

Gene

Rpn1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.By similarity

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (EC:2.4.99.18)
Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit
Ribophorin I
Short name:
RPN-I
Ribophorin-1
Gene namesi
Name:Rpn1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:98084. Rpn1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini26 – 440LumenalSequence analysisAdd BLAST415
Transmembranei441 – 458HelicalSequence analysisAdd BLAST18
Topological domaini459 – 608CytoplasmicSequence analysisAdd BLAST150

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: MGI
  • extracellular matrix Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • melanosome Source: UniProtKB-SubCell
  • membrane Source: MGI
  • oligosaccharyltransferase complex Source: GO_Central
  • rough endoplasmic reticulum Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25By similarityAdd BLAST25
ChainiPRO_000002224226 – 608Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1Add BLAST583

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188N6-acetyllysineBy similarity1
Glycosylationi300N-linked (GlcNAc...)Sequence analysis1
Modified residuei539N6-acetyllysine; alternateCombined sources1
Cross-linki539Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ91YQ5.
MaxQBiQ91YQ5.
PaxDbiQ91YQ5.
PeptideAtlasiQ91YQ5.
PRIDEiQ91YQ5.

PTM databases

iPTMnetiQ91YQ5.
PhosphoSitePlusiQ91YQ5.
SwissPalmiQ91YQ5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030062.
CleanExiMM_RPN1.
ExpressionAtlasiQ91YQ5. baseline and differential.
GenevisibleiQ91YQ5. MM.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi222240. 3 interactors.
IntActiQ91YQ5. 5 interactors.
MINTiMINT-1581523.
STRINGi10090.ENSMUSP00000032143.

Structurei

3D structure databases

ProteinModelPortaliQ91YQ5.
SMRiQ91YQ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the OST1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2291. Eukaryota.
ENOG410XQVZ. LUCA.
GeneTreeiENSGT00390000009630.
HOGENOMiHOG000247000.
HOVERGENiHBG012864.
InParanoidiQ91YQ5.
KOiK12666.
OMAiPYPTHIT.
OrthoDBiEOG091G05K5.
PhylomeDBiQ91YQ5.
TreeFamiTF312988.

Family and domain databases

InterProiIPR007676. Ribophorin_I.
[Graphical view]
PANTHERiPTHR21049. PTHR21049. 1 hit.
PfamiPF04597. Ribophorin_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91YQ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESPVALLLL LLLCLGALAP TPGSASSEAP PLVNEDVKRT VDLSSHLAKV
60 70 80 90 100
TAEVVLVHPG GGSTSRASSF VLALEPELES RLAHLGVQIK GEDEEDNNLE
110 120 130 140 150
VRETKIKGKS GRFFTVKLPV ALDPGSKISV VVETVYTHVL HPYPTQITQS
160 170 180 190 200
EKQFVVFEGN HYFYSPYPTK TQTMRVKLAS RNVESYTKLG NPSRSEDVLD
210 220 230 240 250
YGPFKDIPAY SQDTFKVHYE NNSPFLTITS MTRVIEVSHW GNIAVEENVD
260 270 280 290 300
LKHTGAVLKG PFSRYDYQRQ PDSGISSIRS FKTILPAAAQ DVYYRDEIGN
310 320 330 340 350
VSTSHLLILD DSVEMEIRPR FPLFGGWKTH YIVGYNLPSY EYLYNLGDQY
360 370 380 390 400
ALKMRFVDHV FDEQVIDSLT VKIILPEGAK NIQVDSPYDI SRAPDELHYT
410 420 430 440 450
YLDTFGRPVI VAYKKNLVEQ HIQDIVVHYT FNKVLMLQEP LLVVAAFYIL
460 470 480 490 500
FFTVIIYVRL DFSITKDPAA EARMKVACIT EQVLTLVNKR LGLYRHFDET
510 520 530 540 550
VNRYKQSRDI STLNSGKKSL ETEHKAVTSE IAVLQSRLKT EGSDLCDRVS
560 570 580 590 600
EMQKLDAQVK ELVLKSAVEA ERLVAGKLKK DTYLENEKLS SGKRQELVTK

IDHILDAL
Length:608
Mass (Da):68,528
Last modified:December 1, 2001 - v1
Checksum:iCBDFA74C792A496E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK052130 mRNA. Translation: BAC34853.1.
AK132750 mRNA. Translation: BAE21332.1.
AK151899 mRNA. Translation: BAE30782.1.
AK153867 mRNA. Translation: BAE32221.1.
BC016080 mRNA. Translation: AAH16080.1.
CCDSiCCDS20332.1.
RefSeqiNP_598694.3. NM_133933.4.
UniGeneiMm.188544.

Genome annotation databases

EnsembliENSMUST00000032143; ENSMUSP00000032143; ENSMUSG00000030062.
GeneIDi103963.
KEGGimmu:103963.
UCSCiuc009cva.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK052130 mRNA. Translation: BAC34853.1.
AK132750 mRNA. Translation: BAE21332.1.
AK151899 mRNA. Translation: BAE30782.1.
AK153867 mRNA. Translation: BAE32221.1.
BC016080 mRNA. Translation: AAH16080.1.
CCDSiCCDS20332.1.
RefSeqiNP_598694.3. NM_133933.4.
UniGeneiMm.188544.

3D structure databases

ProteinModelPortaliQ91YQ5.
SMRiQ91YQ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi222240. 3 interactors.
IntActiQ91YQ5. 5 interactors.
MINTiMINT-1581523.
STRINGi10090.ENSMUSP00000032143.

PTM databases

iPTMnetiQ91YQ5.
PhosphoSitePlusiQ91YQ5.
SwissPalmiQ91YQ5.

Proteomic databases

EPDiQ91YQ5.
MaxQBiQ91YQ5.
PaxDbiQ91YQ5.
PeptideAtlasiQ91YQ5.
PRIDEiQ91YQ5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032143; ENSMUSP00000032143; ENSMUSG00000030062.
GeneIDi103963.
KEGGimmu:103963.
UCSCiuc009cva.2. mouse.

Organism-specific databases

CTDi6184.
MGIiMGI:98084. Rpn1.

Phylogenomic databases

eggNOGiKOG2291. Eukaryota.
ENOG410XQVZ. LUCA.
GeneTreeiENSGT00390000009630.
HOGENOMiHOG000247000.
HOVERGENiHBG012864.
InParanoidiQ91YQ5.
KOiK12666.
OMAiPYPTHIT.
OrthoDBiEOG091G05K5.
PhylomeDBiQ91YQ5.
TreeFamiTF312988.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

ChiTaRSiRpn1. mouse.
PROiQ91YQ5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030062.
CleanExiMM_RPN1.
ExpressionAtlasiQ91YQ5. baseline and differential.
GenevisibleiQ91YQ5. MM.

Family and domain databases

InterProiIPR007676. Ribophorin_I.
[Graphical view]
PANTHERiPTHR21049. PTHR21049. 1 hit.
PfamiPF04597. Ribophorin_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPN1_MOUSE
AccessioniPrimary (citable) accession number: Q91YQ5
Secondary accession number(s): Q3U985
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: December 1, 2001
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.