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Protein

Neurolysin, mitochondrial

Gene

Nln

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A.By similarity

Catalytic activityi

Preferential cleavage in neurotensin: 10-Pro-|-Tyr-11.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi497Zinc; catalyticPROSITE-ProRule annotation1
Active sitei498PROSITE-ProRule annotation1
Metal bindingi501Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi504Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: GO_Central
  • peptidase activity Source: MGI
  • peptide binding Source: MGI

GO - Biological processi

  • peptide metabolic process Source: GO_Central
  • regulation of gluconeogenesis Source: MGI
  • regulation of skeletal muscle fiber differentiation Source: MGI

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-375276 Peptide ligand-binding receptors

Protein family/group databases

MEROPSiM03.002

Names & Taxonomyi

Protein namesi
Recommended name:
Neurolysin, mitochondrial (EC:3.4.24.16)
Alternative name(s):
Microsomal endopeptidase
Short name:
MEP
Mitochondrial oligopeptidase M
Neurotensin endopeptidase
Gene namesi
Name:Nln
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1923055 Nln

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 37MitochondrionAdd BLAST37
ChainiPRO_000031904638 – 704Neurolysin, mitochondrialAdd BLAST667

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei664N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ91YP2
MaxQBiQ91YP2
PaxDbiQ91YP2
PeptideAtlasiQ91YP2
PRIDEiQ91YP2

PTM databases

iPTMnetiQ91YP2
PhosphoSitePlusiQ91YP2
SwissPalmiQ91YP2

Expressioni

Gene expression databases

BgeeiENSMUSG00000021710
CleanExiMM_NLN
GenevisibleiQ91YP2 MM

Interactioni

Protein-protein interaction databases

IntActiQ91YP2, 1 interactor
MINTiQ91YP2
STRINGi10090.ENSMUSP00000104938

Structurei

3D structure databases

ProteinModelPortaliQ91YP2
SMRiQ91YP2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2089 Eukaryota
COG0339 LUCA
GeneTreeiENSGT00550000074738
HOGENOMiHOG000245985
HOVERGENiHBG000238
InParanoidiQ91YP2
KOiK01393
OMAiENWVWDI
OrthoDBiEOG091G0325
PhylomeDBiQ91YP2
TreeFamiTF300459

Family and domain databases

Gene3Di1.10.1370.10, 2 hits
1.20.1050.40, 1 hit
3.40.390.10, 1 hit
InterProiView protein in InterPro
IPR024079 MetalloPept_cat_dom_sf
IPR024077 Neurolysin/TOP_dom2
IPR024080 Neurolysin/TOP_N
IPR001567 Pept_M3A_M3B
PfamiView protein in Pfam
PF01432 Peptidase_M3, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91YP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MITLCLSALR GLHRAGGSRI RLRMTLGREA ASPLQAMSSY TAAGRNVLRW
60 70 80 90 100
DLSPEQIRTR TEELIAQTKQ VYDTVGTINL EDVTYENCLQ VLADIEVKYI
110 120 130 140 150
VERTMLDFPQ HVSSDREVRA ASTEADKRLS RFDIEMSMRE DVFQRIVHLQ
160 170 180 190 200
ETCDLEKIKP EARRYLEKSI KMGKRNGLHL PEHVKNEIKS MKKRMSELCI
210 220 230 240 250
DFNKNLNEDD TSLVFSKAEL GALPDDFIDS LEKTDEDKYK VTLKYPHYFP
260 270 280 290 300
VMKKCCVPET RRKMEMAFHT RCKEENTIIL QQLLPLRAQV AKLLGYNTHA
310 320 330 340 350
DFVLELNTAK STSHVATFLD DLSQKLKPLG EAEREFILSL KKKECEERGF
360 370 380 390 400
AYDGKINAWD LHYYMTQTEE LKYSVDQESL KEYFPIEVVT EGLLSIYQEL
410 420 430 440 450
LGLSFEQVAD AHVWNKSVSL YTVKDKATGE VLGQFYLDLY PREGKYNHAA
460 470 480 490 500
CFGLQPGCLL PDGSRMMSVA ALVVNFSQPI AGRPSLLRHD EVRTYFHEFG
510 520 530 540 550
HVMHQICAQT DFARFSGTNV ETDFVEVPSQ MLENWVWDID SLRKLSKHYR
560 570 580 590 600
DGHPITDELL EKLVASRLVN TGLLTLRQIV LSKVDQSLHT NASLDAASEY
610 620 630 640 650
AKYCTEILGV AATPGTNMPA TFGHLAGGYD GQYYGYLWSE VFSMDMFHSC
660 670 680 690 700
FRKEGIMNPE VGMKYRNLIL KPGGSLDGMD MLQNFLQREP NQKAFLMSRG

LNAS
Length:704
Mass (Da):80,429
Last modified:December 1, 2001 - v1
Checksum:iE4F342E346AB0E07
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44G → V in BAE27114 (PubMed:16141072).Curated1
Sequence conflicti329 – 331LGE → HAS in AAH25520 (PubMed:15489334).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK079090 mRNA Translation: BAC37533.1
AK146364 mRNA Translation: BAE27114.1
BC016224 mRNA Translation: AAH16224.1
BC025520 mRNA Translation: AAH25520.1
CCDSiCCDS36772.1
RefSeqiNP_083723.1, NM_029447.2
UniGeneiMm.127692

Genome annotation databases

EnsembliENSMUST00000109315; ENSMUSP00000104938; ENSMUSG00000021710
GeneIDi75805
KEGGimmu:75805
UCSCiuc007rsp.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNEUL_MOUSE
AccessioniPrimary (citable) accession number: Q91YP2
Secondary accession number(s): Q3UJP1, Q8R3F4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: December 1, 2001
Last modified: March 28, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health