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Protein

UDP-N-acetylhexosamine pyrophosphorylase

Gene

Uap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts UTP and GlcNAc-1-P into UDP-GlcNAc, and UTP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P (By similarity).By similarity

Catalytic activityi

UTP + N-acetyl-alpha-D-galactosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-galactosamine.
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. UDP-N-acetylhexosamine pyrophosphorylase (Uap1)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei122UTPBy similarity1
Binding sitei196UTPBy similarity1
Binding sitei222UTP; via amide nitrogenBy similarity1
Binding sitei223SubstrateBy similarity1
Binding sitei253UTPBy similarity1
Binding sitei377UTPBy similarity1
Binding sitei407SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00113; UER00533.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylhexosamine pyrophosphorylase
Including the following 2 domains:
UDP-N-acetylgalactosamine pyrophosphorylase (EC:2.7.7.83)
UDP-N-acetylglucosamine pyrophosphorylase (EC:2.7.7.23)
Gene namesi
Name:Uap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1334459. Uap1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001857681 – 522UDP-N-acetylhexosamine pyrophosphorylaseAdd BLAST522

Proteomic databases

PaxDbiQ91YN5.
PeptideAtlasiQ91YN5.
PRIDEiQ91YN5.

PTM databases

iPTMnetiQ91YN5.
PhosphoSitePlusiQ91YN5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026670.
CleanExiMM_UAP1.

Interactioni

Subunit structurei

Monomer and homodimer. Isoform AGX1 is a homodimer. Isoform AGX2 is a monomer (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027981.

Structurei

Secondary structure

1522
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 11Combined sources9
Turni12 – 14Combined sources3
Helixi16 – 19Combined sources4
Helixi22 – 24Combined sources3
Helixi27 – 38Combined sources12
Helixi44 – 56Combined sources13
Turni57 – 61Combined sources5
Helixi65 – 67Combined sources3
Beta strandi68 – 70Combined sources3
Helixi74 – 76Combined sources3
Beta strandi77 – 79Combined sources3
Turni80 – 82Combined sources3
Helixi86 – 99Combined sources14
Beta strandi103 – 108Combined sources6
Turni114 – 117Combined sources4
Beta strandi119 – 121Combined sources3
Helixi122 – 124Combined sources3
Helixi135 – 154Combined sources20
Beta strandi162 – 166Combined sources5
Turni168 – 170Combined sources3
Helixi171 – 180Combined sources10
Helixi182 – 185Combined sources4
Helixi188 – 190Combined sources3
Beta strandi191 – 195Combined sources5
Beta strandi198 – 200Combined sources3
Beta strandi210 – 212Combined sources3
Beta strandi218 – 220Combined sources3
Helixi225 – 232Combined sources8
Helixi235 – 242Combined sources8
Beta strandi246 – 251Combined sources6
Helixi262 – 270Combined sources9
Beta strandi274 – 281Combined sources8
Beta strandi291 – 295Combined sources5
Beta strandi298 – 302Combined sources5
Turni304 – 306Combined sources3
Helixi309 – 313Combined sources5
Beta strandi319 – 323Combined sources5
Beta strandi325 – 334Combined sources10
Helixi335 – 343Combined sources9
Helixi345 – 348Combined sources4
Beta strandi352 – 356Combined sources5
Beta strandi375 – 379Combined sources5
Helixi382 – 388Combined sources7
Beta strandi390 – 397Combined sources8
Helixi399 – 402Combined sources4
Helixi418 – 435Combined sources18
Beta strandi438 – 441Combined sources4
Beta strandi481 – 483Combined sources3
Turni485 – 487Combined sources3
Beta strandi489 – 491Combined sources3
Helixi495 – 498Combined sources4
Beta strandi501 – 503Combined sources3
Beta strandi505 – 510Combined sources6
Beta strandi513 – 516Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VM8X-ray2.50A/B1-522[»]
ProteinModelPortaliQ91YN5.
SMRiQ91YN5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91YN5.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 111UTP bindingBy similarity4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi108 – 111Substrate bindingBy similarity4
Motifi303 – 304Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the UDPGP type 1 family.Curated

Phylogenomic databases

eggNOGiKOG2388. Eukaryota.
COG4284. LUCA.
HOGENOMiHOG000186273.
HOVERGENiHBG018024.
InParanoidiQ91YN5.
KOiK00972.
PhylomeDBiQ91YN5.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_fam.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 2 hits.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform AGX2 (identifier: Q91YN5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNVNDLKQRL SQAGQEHLLQ FWNELSEAQQ VELYMELQAM NFEELNSFFR
60 70 80 90 100
KAIGEFDRSS HQEKVDARME PVPRQVLGSA TRDQEQLQAW ESEGLSQISQ
110 120 130 140 150
NKVAVLLLAG GQGTRLGVSY PKGMYDVGLP SHKTLFQIQA ERILKLQQLA
160 170 180 190 200
EKHHGNKCTI PWYIMTSGRT MESTKEFFTK HKFFGLKKEN VVFFQQGMLP
210 220 230 240 250
AMSFDGKIIL EEKNKVSMAP DGNGGLYRAL AAQNIVEDME QRGICSIHVY
260 270 280 290 300
CVDNILVKVA DPRFIGFCIQ KGADCGAKVV EKTNPTEPVG VVCRVDGVYQ
310 320 330 340 350
VVEYSEISLA TAQRRSSDGR LLFNAGNIAN HFFTVPFLKD VVNVYEPQLQ
360 370 380 390 400
HHVAQKKIPY VDSQGYFIKP DKPNGIKMEK FVFDIFQFAK KFVVYEVLRE
410 420 430 440 450
DEFSPLKNAD SQNGKDNPTT ARHALMSLHH CWVLNAGGHF IDENGSRLPA
460 470 480 490 500
IPRSATNGKS EAITADVNHN LKDANDVPIQ CEISPLISYA GEGLEGYVAD
510 520
KEFHAPLIID ENGVHELVKN GI
Length:522
Mass (Da):58,609
Last modified:December 1, 2001 - v1
Checksum:i121B4ACCCDDD43B5
GO
Isoform AGX1 (identifier: Q91YN5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     454-470: Missing.

Show »
Length:505
Mass (Da):56,898
Checksum:i47E48D1243198FCB
GO
Isoform 3 (identifier: Q91YN5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     454-454: Missing.

Show »
Length:521
Mass (Da):58,522
Checksum:iBA4CC34659D777D9
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti366Y → H in strain: C57BL/6J. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_014525454 – 470Missing in isoform AGX1. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_014524454Missing in isoform 3. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK047566 mRNA. Translation: BAC33089.1.
AK049519 mRNA. Translation: BAC33791.1.
AK081690 mRNA. Translation: BAC38294.1.
BC016406 mRNA. Translation: AAH16406.1.
BC017547 mRNA. Translation: AAH17547.1.
CCDSiCCDS15467.1. [Q91YN5-3]
RefSeqiNP_001291974.1. NM_001305045.1.
NP_001291975.1. NM_001305046.1.
NP_598567.2. NM_133806.5.
XP_006496671.2. XM_006496608.3.
UniGeneiMm.27969.

Genome annotation databases

GeneIDi107652.
KEGGimmu:107652.
UCSCiuc007dlw.2. mouse. [Q91YN5-3]
uc007dly.2. mouse. [Q91YN5-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK047566 mRNA. Translation: BAC33089.1.
AK049519 mRNA. Translation: BAC33791.1.
AK081690 mRNA. Translation: BAC38294.1.
BC016406 mRNA. Translation: AAH16406.1.
BC017547 mRNA. Translation: AAH17547.1.
CCDSiCCDS15467.1. [Q91YN5-3]
RefSeqiNP_001291974.1. NM_001305045.1.
NP_001291975.1. NM_001305046.1.
NP_598567.2. NM_133806.5.
XP_006496671.2. XM_006496608.3.
UniGeneiMm.27969.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VM8X-ray2.50A/B1-522[»]
ProteinModelPortaliQ91YN5.
SMRiQ91YN5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027981.

PTM databases

iPTMnetiQ91YN5.
PhosphoSitePlusiQ91YN5.

Proteomic databases

PaxDbiQ91YN5.
PeptideAtlasiQ91YN5.
PRIDEiQ91YN5.

Protocols and materials databases

DNASUi107652.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi107652.
KEGGimmu:107652.
UCSCiuc007dlw.2. mouse. [Q91YN5-3]
uc007dly.2. mouse. [Q91YN5-2]

Organism-specific databases

CTDi6675.
MGIiMGI:1334459. Uap1.

Phylogenomic databases

eggNOGiKOG2388. Eukaryota.
COG4284. LUCA.
HOGENOMiHOG000186273.
HOVERGENiHBG018024.
InParanoidiQ91YN5.
KOiK00972.
PhylomeDBiQ91YN5.

Enzyme and pathway databases

UniPathwayiUPA00113; UER00533.

Miscellaneous databases

EvolutionaryTraceiQ91YN5.
PROiQ91YN5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026670.
CleanExiMM_UAP1.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR029044. Nucleotide-diphossugar_trans.
IPR002618. UDPGP_fam.
[Graphical view]
PANTHERiPTHR11952. PTHR11952. 2 hits.
PfamiPF01704. UDPGP. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUAP1_MOUSE
AccessioniPrimary (citable) accession number: Q91YN5
Secondary accession number(s): Q8BG76, Q8BXD6, Q8VD59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.