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Q91YM2

- RHG35_MOUSE

UniProt

Q91YM2 - RHG35_MOUSE

Protein

Rho GTPase-activating protein 35

Gene

Arhgap35

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 3 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor By similarity.By similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. GTPase activator activity Source: MGI
    3. GTP binding Source: InterPro

    GO - Biological processi

    1. camera-type eye development Source: MGI
    2. forebrain development Source: MGI
    3. integrin-mediated signaling pathway Source: MGI
    4. negative regulation of transcription, DNA-templated Source: Ensembl
    5. negative regulation of vascular permeability Source: MGI
    6. neural tube closure Source: MGI
    7. positive regulation of GTPase activity Source: GOC
    8. regulation of cell shape Source: MGI
    9. small GTPase mediated signal transduction Source: InterPro
    10. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    GTPase activation, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_210090. Rho GTPase cycle.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rho GTPase-activating protein 35
    Alternative name(s):
    Glucocorticoid receptor DNA-binding factor 1
    Gene namesi
    Name:Arhgap35
    Synonyms:Grlf1,Kiaa1722
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1929494. Arhgap35.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. actin cytoskeleton Source: MGI
    2. cytoplasm Source: MGI
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Keywords - Diseasei

    Tumor suppressor

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14991499Rho GTPase-activating protein 35PRO_0000056731Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei308 – 3081PhosphotyrosineBy similarity
    Modified residuei589 – 5891PhosphoserineBy similarity
    Modified residuei773 – 7731PhosphoserineBy similarity
    Modified residuei970 – 9701PhosphoserineBy similarity
    Modified residuei975 – 9751PhosphoserineBy similarity
    Modified residuei1072 – 10721PhosphoserineBy similarity
    Modified residuei1087 – 10871PhosphotyrosineBy similarity
    Modified residuei1105 – 11051Phosphotyrosine; by ABL2 and PTK63 Publications
    Modified residuei1134 – 11341PhosphoserineBy similarity
    Modified residuei1150 – 11501PhosphoserineBy similarity
    Modified residuei1179 – 11791PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation of Tyr-1105 by PTK6 promotes the association with RASA1, inactivating RHOA while activating RAS. Phosphorylation at Tyr-308 by PDGFRA inhibits binding to GTF2I By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ91YM2.
    PaxDbiQ91YM2.
    PRIDEiQ91YM2.

    PTM databases

    PhosphoSiteiQ91YM2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ91YM2.
    BgeeiQ91YM2.
    CleanExiMM_GRLF1.
    GenevestigatoriQ91YM2.

    Interactioni

    Subunit structurei

    Interacts with the general transcription factor GTF2I, the interaction sequesters GTF2I in the cytoplasm By similarity. Interacts with RASA1.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi231317. 2 interactions.
    IntActiQ91YM2. 1 interaction.
    MINTiMINT-4096884.
    STRINGi10090.ENSMUSP00000075242.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91YM2.
    SMRiQ91YM2. Positions 13-249, 267-331, 1242-1437.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini270 – 32758FF 1Add
    BLAST
    Domaini368 – 42255FF 2Add
    BLAST
    Domaini429 – 48355FF 3Add
    BLAST
    Domaini485 – 55066FF 4Add
    BLAST
    Domaini1249 – 1436188Rho-GAPPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1440 – 148748Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 4 FF domains.Curated
    Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG241832.
    GeneTreeiENSGT00740000114884.
    HOVERGENiHBG051844.
    InParanoidiQ91YM2.
    KOiK05732.
    OMAiVLYRTRC.
    OrthoDBiEOG779NWX.
    PhylomeDBiQ91YM2.
    TreeFamiTF324451.

    Family and domain databases

    Gene3Di1.10.555.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR002713. FF_domain.
    IPR027417. P-loop_NTPase.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR001806. Small_GTPase.
    [Graphical view]
    PfamiPF01846. FF. 1 hit.
    PF00071. Ras. 1 hit.
    PF00620. RhoGAP. 1 hit.
    [Graphical view]
    SMARTiSM00441. FF. 4 hits.
    SM00324. RhoGAP. 1 hit.
    [Graphical view]
    SUPFAMiSSF48350. SSF48350. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF81698. SSF81698. 1 hit.
    PROSITEiPS51676. FF. 4 hits.
    PS50238. RHOGAP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q91YM2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMMARKQDVR IPTYNISVVG LSGTEKEKGQ CGIGKSCLCN RFVRPSADEF     50
    HLDHTSVLST SDFGGRVVNN DHFLYWGEVS RSLEDCVECK MHIVEQTEFI 100
    DDQTFQPHRS TALQPYIKRA AATKLASAEK LMYFCTDQLG LEQDFEQKQM 150
    PDGKLLVDGF LLGIDVSRGM NRNFDDQLKF VSNLYNQLAK TKKPIVVVLT 200
    KCDEGVERYI RDAHTFALSK KNLQVVETSA RSNVNVDLAF STLVQLIDKS 250
    RGKTKIIPYF EALKQQSQQI ATAKDKYEWL VSRIVKNHNE NWPSVSRKMQ 300
    ASPEYQDYVY LEGTQKAKKL FLQHIHRLKH EHIERRRKLY LAALPLAFEA 350
    LIPNLDEVDH LSCIKAKKLL ETKPEFLKWF VVLEETPWDA TSHIDNMENE 400
    RIPFDLMDTV PAEQLYETHL EKLRNERKRA EMRRAFKENL ETSPFITPGK 450
    PWEEARSFIM NEDFYQWLEE SVYMDIYGKH QKQIIDRAKE EFQELLLEYS 500
    ELFYELELDA KPSKEKMGVI QDVLGEEQRF KALQKLQAER DALILKHIHF 550
    VYHPTKETCP SCPACVDAKI EHLISSRFIR PSDRNQKNSL SDLNIDRINL 600
    VILGKDGLAR ELANEIRALC TNDDKYVIDG KMYELSLRPI EGNVRLPVNS 650
    FQTPTFQPHG CLCLYNSKES LSYVVESIEK SRESTLGRRD NHLVHLPLTL 700
    ILVNKRGDTS GETLHSLIQQ GQQIASKLQC VFLDPASAGI GYGRNINEKQ 750
    ISQVLKGLLD SKRNLNLVSS TASIKDLADV DLRIVMCLMC GDPFSADDVL 800
    SPVLQSQTCK SSHCGSSNSV LLELPIGLHK KRIELSVLSY HSSFSIRKSR 850
    LVHGYIVFYS AKRKASLAML RAFLCEVQDI IPIQLVALTD GAIDVLDNDL 900
    SREQLTEGEE IAQEIDGRFT SIPCSQPQHK LELFHPFFKD VVEKKNIIEA 950
    THMYDNVAEA CSTTEEVFNS PRAGSPLCNS NLQDSEEDVE PPSYHLFRED 1000
    ATLPSLSKDH SKFSMELEGN DGLSFIMSNF ESKLNNKVPP PVKPKPPVHF 1050
    DITKDLSYLD QGHREGQRKS MSSSPWMPQD GFDPSDYAEP MDAVVKPRNE 1100
    EENIYSVPHD STQGKIITIR NINKAQSNGS GNGSDSEMDT SSLERGRKVS 1150
    AVSKPVLYRT RCTRLGRFAS YRTSFSVGSD DELGPIRKKE EDQASQGYKG 1200
    DNAVIPYETD EDPRRRNILR SLRRNTKKPK PKPRPSITKA TWESNYFGVP 1250
    LTTVVTPEKP IPIFIERCIE YIEATGLSTE GIYRVSGNKS EMESLQRQFD 1300
    QDHNLDLAEK DFTVNTVAGA MKSFFSELPD PLVPYSMQID LVEAHKINDR 1350
    EQKLHALKEV LKKFPKENHE VFKYVISHLN KVSHNNKVNL MTSENLSICF 1400
    WPTLMRPDFS SMDALTATRS YQTIIELFIQ QCPFFFYNRP ISEPPGAAPG 1450
    SPSAMAPTVP FLTSTPATSQ PSPPQSPPPT PQSPMQPLLS SQLQAEHTL 1499
    Length:1,499
    Mass (Da):170,393
    Last modified:January 9, 2007 - v3
    Checksum:i7E13EC38257CE950
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK147326 mRNA. Translation: BAE27848.1.
    AK147688 mRNA. Translation: BAE28076.1.
    AK173242 Transcribed RNA. Translation: BAD32520.1.
    CCDSiCCDS20851.1.
    RefSeqiNP_766327.3. NM_172739.4.
    XP_006539871.1. XM_006539808.1.
    XP_006539872.1. XM_006539809.1.
    XP_006539873.1. XM_006539810.1.
    UniGeneiMm.28646.
    Mm.400358.

    Genome annotation databases

    EnsembliENSMUST00000075845; ENSMUSP00000075242; ENSMUSG00000058230.
    ENSMUST00000171937; ENSMUSP00000127379; ENSMUSG00000058230.
    GeneIDi232906.
    KEGGimmu:232906.
    UCSCiuc009fhw.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK147326 mRNA. Translation: BAE27848.1 .
    AK147688 mRNA. Translation: BAE28076.1 .
    AK173242 Transcribed RNA. Translation: BAD32520.1 .
    CCDSi CCDS20851.1.
    RefSeqi NP_766327.3. NM_172739.4.
    XP_006539871.1. XM_006539808.1.
    XP_006539872.1. XM_006539809.1.
    XP_006539873.1. XM_006539810.1.
    UniGenei Mm.28646.
    Mm.400358.

    3D structure databases

    ProteinModelPortali Q91YM2.
    SMRi Q91YM2. Positions 13-249, 267-331, 1242-1437.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 231317. 2 interactions.
    IntActi Q91YM2. 1 interaction.
    MINTi MINT-4096884.
    STRINGi 10090.ENSMUSP00000075242.

    PTM databases

    PhosphoSitei Q91YM2.

    Proteomic databases

    MaxQBi Q91YM2.
    PaxDbi Q91YM2.
    PRIDEi Q91YM2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000075845 ; ENSMUSP00000075242 ; ENSMUSG00000058230 .
    ENSMUST00000171937 ; ENSMUSP00000127379 ; ENSMUSG00000058230 .
    GeneIDi 232906.
    KEGGi mmu:232906.
    UCSCi uc009fhw.1. mouse.

    Organism-specific databases

    CTDi 2909.
    MGIi MGI:1929494. Arhgap35.

    Phylogenomic databases

    eggNOGi NOG241832.
    GeneTreei ENSGT00740000114884.
    HOVERGENi HBG051844.
    InParanoidi Q91YM2.
    KOi K05732.
    OMAi VLYRTRC.
    OrthoDBi EOG779NWX.
    PhylomeDBi Q91YM2.
    TreeFami TF324451.

    Enzyme and pathway databases

    Reactomei REACT_210090. Rho GTPase cycle.

    Miscellaneous databases

    NextBioi 381321.
    PROi Q91YM2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q91YM2.
    Bgeei Q91YM2.
    CleanExi MM_GRLF1.
    Genevestigatori Q91YM2.

    Family and domain databases

    Gene3Di 1.10.555.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR002713. FF_domain.
    IPR027417. P-loop_NTPase.
    IPR008936. Rho_GTPase_activation_prot.
    IPR000198. RhoGAP_dom.
    IPR001806. Small_GTPase.
    [Graphical view ]
    Pfami PF01846. FF. 1 hit.
    PF00071. Ras. 1 hit.
    PF00620. RhoGAP. 1 hit.
    [Graphical view ]
    SMARTi SM00441. FF. 4 hits.
    SM00324. RhoGAP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48350. SSF48350. 1 hit.
    SSF52540. SSF52540. 2 hits.
    SSF81698. SSF81698. 1 hit.
    PROSITEi PS51676. FF. 4 hits.
    PS50238. RHOGAP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    2. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
      DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 163-1499.
      Tissue: Brain.
    3. "Integrin signaling through Arg activates p190RhoGAP by promoting its binding to p120RasGAP and recruitment to the membrane."
      Bradley W.D., Hernandez S.E., Settleman J., Koleske A.J.
      Mol. Biol. Cell 17:4827-4836(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT TYR-1105, INTERACTION WITH RASA1, FUNCTION.
    4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    5. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
      Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
      J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1105, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Mast cell.
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1105, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiRHG35_MOUSE
    AccessioniPrimary (citable) accession number: Q91YM2
    Secondary accession number(s): Q3UGY1, Q69ZC4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2003
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 120 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3