Rho GTPase-activating protein 35 - Mus musculus (Mouse)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Rho GTPase-activating protein 35

Alternative name(s):
Glucocorticoid receptor DNA-binding factor 1

Gene names

Name:	Arhgap35
Synonyms:	Grlf1,Kiaa1722

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1499 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor By similarity. Ref.7
Subunit structure	Interacts with RASA1. Ref.7
Subcellular location	Nucleus Potential.
Post-translational modification	Phosphorylation of Tyr-1105 by PTK6 promotes the association with RASA1, inactivating RHOA while activating RAS By similarity.
Sequence similarities	Contains 4 FF domains. Contains 1 Rho-GAP domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Disease	Tumor suppressor
Domain	Repeat
Ligand	DNA-binding
Molecular function	GTPase activation Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	camera-type eye development Inferred from mutant phenotype PubMed 11044403. Source: MGI forebrain development Inferred from mutant phenotype PubMed 11044403. Source: MGI integrin-mediated signaling pathway Traceable author statement PubMed 11044403. Source: MGI negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara negative regulation of vascular permeability Inferred from mutant phenotype PubMed 17562701. Source: MGI neural tube closure Inferred from mutant phenotype PubMed 11044403. Source: MGI regulation of cell shape Inferred from direct assay PubMed 11044403. Source: MGI small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	actin cytoskeleton Inferred from direct assay PubMed 11044403. Source: MGI cytoplasm Inferred from direct assay PubMed 11044403. Source: MGI nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: InterPro GTPase activator activity Traceable author statement PubMed 11044403. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1499

1499

Rho GTPase-activating protein 35

PRO_0000056731

Regions

Domain

270 – 327

58

FF 1

Domain

369 – 422

54

FF 2

Domain

429 – 483

55

FF 3

Domain

485 – 539

55

FF 4

Domain

1249 – 1436

188

Rho-GAP

Compositional bias

1440 – 1487

48

Pro-rich

Amino acid modifications

Modified residue

589

1

Phosphoserine By similarity

Modified residue

773

1

Phosphoserine Ref.6

Modified residue

970

1

Phosphoserine By similarity

Modified residue

975

1

Phosphoserine By similarity

Modified residue

980

1

Phosphoserine Ref.6

Modified residue

1072

1

Phosphoserine By similarity

Modified residue

1087

1

Phosphotyrosine Ref.5

Modified residue

1105

1

Phosphotyrosine; by ABL2 and PTK6 Ref.3 Ref.4 Ref.5 Ref.7

Modified residue

1134

1

Phosphoserine By similarity

Modified residue

1150

1

Phosphoserine By similarity

Modified residue

1179

1

Phosphoserine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q91YM2 [UniParc].

Last modified January 9, 2007. Version 3.
Checksum: 7E13EC38257CE950
Show »

FASTA

1,499

170,393

        10         20         30         40         50         60 
MMMARKQDVR IPTYNISVVG LSGTEKEKGQ CGIGKSCLCN RFVRPSADEF HLDHTSVLST 

        70         80         90        100        110        120 
SDFGGRVVNN DHFLYWGEVS RSLEDCVECK MHIVEQTEFI DDQTFQPHRS TALQPYIKRA 

       130        140        150        160        170        180 
AATKLASAEK LMYFCTDQLG LEQDFEQKQM PDGKLLVDGF LLGIDVSRGM NRNFDDQLKF 

       190        200        210        220        230        240 
VSNLYNQLAK TKKPIVVVLT KCDEGVERYI RDAHTFALSK KNLQVVETSA RSNVNVDLAF 

       250        260        270        280        290        300 
STLVQLIDKS RGKTKIIPYF EALKQQSQQI ATAKDKYEWL VSRIVKNHNE NWPSVSRKMQ 

       310        320        330        340        350        360 
ASPEYQDYVY LEGTQKAKKL FLQHIHRLKH EHIERRRKLY LAALPLAFEA LIPNLDEVDH 

       370        380        390        400        410        420 
LSCIKAKKLL ETKPEFLKWF VVLEETPWDA TSHIDNMENE RIPFDLMDTV PAEQLYETHL 

       430        440        450        460        470        480 
EKLRNERKRA EMRRAFKENL ETSPFITPGK PWEEARSFIM NEDFYQWLEE SVYMDIYGKH 

       490        500        510        520        530        540 
QKQIIDRAKE EFQELLLEYS ELFYELELDA KPSKEKMGVI QDVLGEEQRF KALQKLQAER 

       550        560        570        580        590        600 
DALILKHIHF VYHPTKETCP SCPACVDAKI EHLISSRFIR PSDRNQKNSL SDLNIDRINL 

       610        620        630        640        650        660 
VILGKDGLAR ELANEIRALC TNDDKYVIDG KMYELSLRPI EGNVRLPVNS FQTPTFQPHG 

       670        680        690        700        710        720 
CLCLYNSKES LSYVVESIEK SRESTLGRRD NHLVHLPLTL ILVNKRGDTS GETLHSLIQQ 

       730        740        750        760        770        780 
GQQIASKLQC VFLDPASAGI GYGRNINEKQ ISQVLKGLLD SKRNLNLVSS TASIKDLADV 

       790        800        810        820        830        840 
DLRIVMCLMC GDPFSADDVL SPVLQSQTCK SSHCGSSNSV LLELPIGLHK KRIELSVLSY 

       850        860        870        880        890        900 
HSSFSIRKSR LVHGYIVFYS AKRKASLAML RAFLCEVQDI IPIQLVALTD GAIDVLDNDL 

       910        920        930        940        950        960 
SREQLTEGEE IAQEIDGRFT SIPCSQPQHK LELFHPFFKD VVEKKNIIEA THMYDNVAEA 

       970        980        990       1000       1010       1020 
CSTTEEVFNS PRAGSPLCNS NLQDSEEDVE PPSYHLFRED ATLPSLSKDH SKFSMELEGN 

      1030       1040       1050       1060       1070       1080 
DGLSFIMSNF ESKLNNKVPP PVKPKPPVHF DITKDLSYLD QGHREGQRKS MSSSPWMPQD 

      1090       1100       1110       1120       1130       1140 
GFDPSDYAEP MDAVVKPRNE EENIYSVPHD STQGKIITIR NINKAQSNGS GNGSDSEMDT 

      1150       1160       1170       1180       1190       1200 
SSLERGRKVS AVSKPVLYRT RCTRLGRFAS YRTSFSVGSD DELGPIRKKE EDQASQGYKG 

      1210       1220       1230       1240       1250       1260 
DNAVIPYETD EDPRRRNILR SLRRNTKKPK PKPRPSITKA TWESNYFGVP LTTVVTPEKP 

      1270       1280       1290       1300       1310       1320 
IPIFIERCIE YIEATGLSTE GIYRVSGNKS EMESLQRQFD QDHNLDLAEK DFTVNTVAGA 

      1330       1340       1350       1360       1370       1380 
MKSFFSELPD PLVPYSMQID LVEAHKINDR EQKLHALKEV LKKFPKENHE VFKYVISHLN 

      1390       1400       1410       1420       1430       1440 
KVSHNNKVNL MTSENLSICF WPTLMRPDFS SMDALTATRS YQTIIELFIQ QCPFFFYNRP 

      1450       1460       1470       1480       1490 
ISEPPGAAPG SPSAMAPTVP FLTSTPATSQ PSPPQSPPPT PQSPMQPLLS SQLQAEHTL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 163-1499. Tissue: Brain.
[3]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1105, MASS SPECTROMETRY. Tissue: Brain.
[4]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1105, MASS SPECTROMETRY. Tissue: Mast cell.
[5]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-1087 AND TYR-1105, MASS SPECTROMETRY. Tissue: Brain.
[6]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-773 AND SER-980, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Integrin signaling through Arg activates p190RhoGAP by promoting its binding to p120RasGAP and recruitment to the membrane." Bradley W.D., Hernandez S.E., Settleman J., Koleske A.J. Mol. Biol. Cell 17:4827-4836(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT TYR-1105, INTERACTION WITH RASA1, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK147326 mRNA. Translation: BAE27848.1. AK147688 mRNA. Translation: BAE28076.1. AK173242 Transcribed RNA. Translation: BAD32520.1.
IPI	IPI00480321.
RefSeq	NP_766327.3. NM_172739.4.
UniGene	Mm.28646. Mm.400358.
3D structure databases
ProteinModelPortal	Q91YM2.
SMR	Q91YM2. Positions 13-249, 267-331, 1242-1437.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000075242.
PTM databases
PhosphoSite	Q91YM2.
Proteomic databases
PaxDb	Q91YM2.
PRIDE	Q91YM2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000075845; ENSMUSP00000075242; ENSMUSG00000058230. ENSMUST00000171937; ENSMUSP00000127379; ENSMUSG00000058230.
GeneID	232906.
KEGG	mmu:232906.
Organism-specific databases
CTD	232906.
MGI	MGI:1929494. Grlf1.
Phylogenomic databases
eggNOG	NOG241832.
GeneTree	ENSGT00690000101648.
HOVERGEN	HBG051844.
InParanoid	Q91YM2.
KO	K05732.
OMA	LYRTRCS.
OrthoDB	EOG4DZ1TC.
Gene expression databases
ArrayExpress	Q91YM2.
Bgee	Q91YM2.
CleanEx	MM_GRLF1.
Genevestigator	Q91YM2.
GermOnline	ENSMUSG00000058230. Mus musculus.
Family and domain databases
Gene3D	1.10.555.10. 1 hit.
InterPro	IPR002713. FF_domain. IPR008936. Rho_GTPase_activation_prot. IPR000198. RhoGAP_dom. IPR001806. Small_GTPase. [Graphical view]
Pfam	PF01846. FF. 1 hit. PF00071. Ras. 1 hit. PF00620. RhoGAP. 1 hit. [Graphical view]
SMART	SM00441. FF. 4 hits. SM00324. RhoGAP. 1 hit. [Graphical view]
SUPFAM	SSF48350. Rho_GAP. 1 hit.
PROSITE	PS50238. RHOGAP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	381321.
SOURCE	Search...

Entry information

Entry name

RHG35_MOUSE

Accession

Primary (citable) accession number: Q91YM2
Secondary accession number(s): Q3UGY1, Q69ZC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2003
Last sequence update:	January 9, 2007
Last modified:	April 3, 2013
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents