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Q91YI6

- GLD2_MOUSE

UniProt

Q91YI6 - GLD2_MOUSE

Protein

Poly(A) RNA polymerase GLD2

Gene

Papd4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation By similarity.By similarity

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).1 Publication

    Cofactori

    Magnesium or manganese.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi213 – 2131Magnesium or manganese; catalyticBy similarity
    Metal bindingi215 – 2151Magnesium or manganese; catalyticBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. polynucleotide adenylyltransferase activity Source: UniProtKB-EC
    4. protein binding Source: MGI

    GO - Biological processi

    1. hematopoietic progenitor cell differentiation Source: MGI
    2. histone mRNA catabolic process Source: UniProtKB
    3. mRNA processing Source: UniProtKB-KW
    4. RNA polyadenylation Source: Ensembl

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) RNA polymerase GLD2 (EC:2.7.7.19)
    Short name:
    mGLD-2
    Alternative name(s):
    PAP-associated domain-containing protein 4
    Gene namesi
    Name:Papd4
    Synonyms:Gld2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:2140950. Papd4.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice are normal and healthy. Poly-A tail elongation in oocytes is not affected and mice are fertile.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi215 – 2151D → A: Loss of enzyme activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 484484Poly(A) RNA polymerase GLD2PRO_0000341550Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei62 – 621PhosphoserineBy similarity
    Modified residuei69 – 691PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ91YI6.
    PRIDEiQ91YI6.

    PTM databases

    PhosphoSiteiQ91YI6.

    Expressioni

    Tissue specificityi

    Ubiquitous. In brain, it is highly expressed in the cerebral cortex, cerebellum, hippocampus and olfactory bulb.2 Publications

    Developmental stagei

    Expressed in oocytes from metaphase I to metaphase II during oocyte maturation.1 Publication

    Gene expression databases

    BgeeiQ91YI6.
    GenevestigatoriQ91YI6.

    Interactioni

    Subunit structurei

    Interacts with CPEB1, CPEB2, CPSF1 and PABPC1.1 Publication

    Protein-protein interaction databases

    BioGridi221516. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91YI6.
    SMRiQ91YI6. Positions 162-469.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini386 – 44055PAP-associatedAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi76 – 9217Nuclear localization signalSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PAP-associated domain.Curated

    Phylogenomic databases

    eggNOGiCOG5260.
    GeneTreeiENSGT00550000074490.
    HOGENOMiHOG000088606.
    HOVERGENiHBG097533.
    InParanoidiQ91YI6.
    KOiK14079.
    OMAiCVEFDLN.
    OrthoDBiEOG7DRJ2S.
    PhylomeDBiQ91YI6.
    TreeFamiTF315661.

    Family and domain databases

    InterProiIPR002058. PAP_assoc.
    [Graphical view]
    PfamiPF03828. PAP_assoc. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q91YI6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFPNSILGRP PFTPTHQQHN NFFALSPTLY SHQQLIDAQF NFQNVDLSRA    50
    VSLQPLTYGT VSPIQTSTSP LFRGRKRISD EKAFPLDGKR QRFHSPHQEP 100
    TIINQLVPLS GDRRYSMPPL FHTHYIPDIV RCVPPLREIP LLEPREITLP 150
    EAKDKLSQQI LELFETCQQQ ASDLKKKELC RAQLQREIQL LFPQSRLFLV 200
    GSSLNGFGAR SSDGDLCLVV KEEPCFFQVN QKTEARHILT LVHKHFCTRL 250
    SGYIERPQLI RAKVPIVKFR DKVSCVEFDL NVNNTVGIRN TFLLRTYAYL 300
    ENRVRPLVLV IKKWASHHDI NDASRGTLSS YSLVLMVLHY LQTLPEPILP 350
    SLQKIYPESF STSVQLHLVH HAPCNVPPYL SKNESSLGDL LLGFLKYYAT 400
    EFDWNTQMIS VREAKAIPRP DDMEWRNKYI CVEEPFDGTN TARAVHEKQK 450
    FDMIKDQFLK SWQRLKNKRD LNSVLPLRAA TLKR 484
    Length:484
    Mass (Da):55,934
    Last modified:December 1, 2001 - v1
    Checksum:iC60D799AA5F14CB6
    GO
    Isoform 2 (identifier: Q91YI6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         225-228: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:480
    Mass (Da):55,408
    Checksum:i7CD85BCCF29AB625
    GO

    Sequence cautioni

    The sequence BAC28170.1 differs from that shown. Reason: Frameshift at position 216.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti357 – 3571P → T in BAC28170. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei225 – 2284Missing in isoform 2. 1 PublicationVSP_034325

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM419011 mRNA. Translation: CAL91354.1.
    BC016629 mRNA. Translation: AAH16629.1.
    AK033141 mRNA. Translation: BAC28170.1. Frameshift.
    CCDSiCCDS26686.1. [Q91YI6-1]
    RefSeqiNP_598666.1. NM_133905.2. [Q91YI6-1]
    XP_006517573.1. XM_006517510.1. [Q91YI6-1]
    XP_006517574.1. XM_006517511.1. [Q91YI6-2]
    UniGeneiMm.242865.

    Genome annotation databases

    EnsembliENSMUST00000048702; ENSMUSP00000048124; ENSMUSG00000042167. [Q91YI6-1]
    GeneIDi100715.
    KEGGimmu:100715.
    UCSCiuc007rkz.1. mouse. [Q91YI6-2]
    uc007rla.1. mouse. [Q91YI6-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM419011 mRNA. Translation: CAL91354.1 .
    BC016629 mRNA. Translation: AAH16629.1 .
    AK033141 mRNA. Translation: BAC28170.1 . Frameshift.
    CCDSi CCDS26686.1. [Q91YI6-1 ]
    RefSeqi NP_598666.1. NM_133905.2. [Q91YI6-1 ]
    XP_006517573.1. XM_006517510.1. [Q91YI6-1 ]
    XP_006517574.1. XM_006517511.1. [Q91YI6-2 ]
    UniGenei Mm.242865.

    3D structure databases

    ProteinModelPortali Q91YI6.
    SMRi Q91YI6. Positions 162-469.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 221516. 2 interactions.

    PTM databases

    PhosphoSitei Q91YI6.

    Proteomic databases

    MaxQBi Q91YI6.
    PRIDEi Q91YI6.

    Protocols and materials databases

    DNASUi 100715.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000048702 ; ENSMUSP00000048124 ; ENSMUSG00000042167 . [Q91YI6-1 ]
    GeneIDi 100715.
    KEGGi mmu:100715.
    UCSCi uc007rkz.1. mouse. [Q91YI6-2 ]
    uc007rla.1. mouse. [Q91YI6-1 ]

    Organism-specific databases

    CTDi 167153.
    MGIi MGI:2140950. Papd4.

    Phylogenomic databases

    eggNOGi COG5260.
    GeneTreei ENSGT00550000074490.
    HOGENOMi HOG000088606.
    HOVERGENi HBG097533.
    InParanoidi Q91YI6.
    KOi K14079.
    OMAi CVEFDLN.
    OrthoDBi EOG7DRJ2S.
    PhylomeDBi Q91YI6.
    TreeFami TF315661.

    Miscellaneous databases

    ChiTaRSi PAPD4. mouse.
    NextBioi 354606.
    PROi Q91YI6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q91YI6.
    Genevestigatori Q91YI6.

    Family and domain databases

    InterProi IPR002058. PAP_assoc.
    [Graphical view ]
    Pfami PF03828. PAP_assoc. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "XRbm9, a new XGld2-interacting protein, enhances translation in Xenopus oocytes."
      Papin C.
      Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: NIH Swiss.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Mammary tumor.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 147-484.
      Strain: C57BL/6J.
      Tissue: Testis.
    4. Cited for: ENZYME ACTIVITY.
    5. "Vertebrate GLD2 poly(A) polymerases in the germline and the brain."
      Rouhana L., Wang L., Buter N., Kwak J.E., Schiltz C.A., Gonzalez T., Kelley A.E., Landry C.F., Wickens M.
      RNA 11:1117-1130(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "Possible role of mouse poly(A) polymerase mGLD-2 during oocyte maturation."
      Nakanishi T., Kubota H., Ishibashi N., Kumagai S., Watanabe H., Yamashita M., Kashiwabara S., Miyado K., Baba T.
      Dev. Biol. 289:115-126(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF ASP-215.
    7. "Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells."
      Nakanishi T., Kumagai S., Kimura M., Watanabe H., Sakurai T., Kimura M., Kashiwabara S., Baba T.
      Biochem. Biophys. Res. Commun. 364:14-19(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, INTERACTION WITH CPEB1; CPEB2; CPSF1 AND PABPC1.

    Entry informationi

    Entry nameiGLD2_MOUSE
    AccessioniPrimary (citable) accession number: Q91YI6
    Secondary accession number(s): A4F5H0, Q8CCH5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 10, 2008
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3