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Q91YI6

- GLD2_MOUSE

UniProt

Q91YI6 - GLD2_MOUSE

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Protein

Poly(A) RNA polymerase GLD2

Gene

Papd4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytoplasmic poly(A) RNA polymerase that adds successive AMP monomers to the 3'-end of specific RNAs, forming a poly(A) tail. In contrast to the canonical nuclear poly(A) RNA polymerase, it only adds poly(A) to selected cytoplasmic mRNAs. Does not play a role in replication-dependent histone mRNA degradation (By similarity).By similarity

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi213 – 2131Magnesium or manganese; catalyticBy similarity
Metal bindingi215 – 2151Magnesium or manganese; catalyticBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. hematopoietic progenitor cell differentiation Source: MGI
  2. histone mRNA catabolic process Source: UniProtKB
  3. mRNA processing Source: UniProtKB-KW
  4. RNA polyadenylation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) RNA polymerase GLD2 (EC:2.7.7.19)
Short name:
mGLD-2
Alternative name(s):
PAP-associated domain-containing protein 4
Gene namesi
Name:Papd4
Synonyms:Gld2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:2140950. Papd4.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are normal and healthy. Poly-A tail elongation in oocytes is not affected and mice are fertile.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi215 – 2151D → A: Loss of enzyme activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484Poly(A) RNA polymerase GLD2PRO_0000341550Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei62 – 621PhosphoserineBy similarity
Modified residuei69 – 691PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ91YI6.
PRIDEiQ91YI6.

PTM databases

PhosphoSiteiQ91YI6.

Expressioni

Tissue specificityi

Ubiquitous. In brain, it is highly expressed in the cerebral cortex, cerebellum, hippocampus and olfactory bulb.2 Publications

Developmental stagei

Expressed in oocytes from metaphase I to metaphase II during oocyte maturation.1 Publication

Gene expression databases

BgeeiQ91YI6.
GenevestigatoriQ91YI6.

Interactioni

Subunit structurei

Interacts with CPEB1, CPEB2, CPSF1 and PABPC1.1 Publication

Protein-protein interaction databases

BioGridi221516. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ91YI6.
SMRiQ91YI6. Positions 162-469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini386 – 44055PAP-associatedAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi76 – 9217Nuclear localization signalSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 PAP-associated domain.Curated

Phylogenomic databases

eggNOGiCOG5260.
GeneTreeiENSGT00550000074490.
HOGENOMiHOG000088606.
HOVERGENiHBG097533.
InParanoidiQ91YI6.
KOiK14079.
OMAiCVEFDLN.
OrthoDBiEOG7DRJ2S.
PhylomeDBiQ91YI6.
TreeFamiTF315661.

Family and domain databases

InterProiIPR002058. PAP_assoc.
[Graphical view]
PfamiPF03828. PAP_assoc. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q91YI6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFPNSILGRP PFTPTHQQHN NFFALSPTLY SHQQLIDAQF NFQNVDLSRA
60 70 80 90 100
VSLQPLTYGT VSPIQTSTSP LFRGRKRISD EKAFPLDGKR QRFHSPHQEP
110 120 130 140 150
TIINQLVPLS GDRRYSMPPL FHTHYIPDIV RCVPPLREIP LLEPREITLP
160 170 180 190 200
EAKDKLSQQI LELFETCQQQ ASDLKKKELC RAQLQREIQL LFPQSRLFLV
210 220 230 240 250
GSSLNGFGAR SSDGDLCLVV KEEPCFFQVN QKTEARHILT LVHKHFCTRL
260 270 280 290 300
SGYIERPQLI RAKVPIVKFR DKVSCVEFDL NVNNTVGIRN TFLLRTYAYL
310 320 330 340 350
ENRVRPLVLV IKKWASHHDI NDASRGTLSS YSLVLMVLHY LQTLPEPILP
360 370 380 390 400
SLQKIYPESF STSVQLHLVH HAPCNVPPYL SKNESSLGDL LLGFLKYYAT
410 420 430 440 450
EFDWNTQMIS VREAKAIPRP DDMEWRNKYI CVEEPFDGTN TARAVHEKQK
460 470 480
FDMIKDQFLK SWQRLKNKRD LNSVLPLRAA TLKR
Length:484
Mass (Da):55,934
Last modified:December 1, 2001 - v1
Checksum:iC60D799AA5F14CB6
GO
Isoform 2 (identifier: Q91YI6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     225-228: Missing.

Note: No experimental confirmation available.

Show »
Length:480
Mass (Da):55,408
Checksum:i7CD85BCCF29AB625
GO

Sequence cautioni

The sequence BAC28170.1 differs from that shown. Reason: Frameshift at position 216. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti357 – 3571P → T in BAC28170. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei225 – 2284Missing in isoform 2. 1 PublicationVSP_034325

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM419011 mRNA. Translation: CAL91354.1.
BC016629 mRNA. Translation: AAH16629.1.
AK033141 mRNA. Translation: BAC28170.1. Frameshift.
CCDSiCCDS26686.1. [Q91YI6-1]
RefSeqiNP_598666.1. NM_133905.2. [Q91YI6-1]
XP_006517573.1. XM_006517510.1. [Q91YI6-1]
XP_006517574.1. XM_006517511.1. [Q91YI6-2]
UniGeneiMm.242865.

Genome annotation databases

EnsembliENSMUST00000048702; ENSMUSP00000048124; ENSMUSG00000042167. [Q91YI6-1]
GeneIDi100715.
KEGGimmu:100715.
UCSCiuc007rkz.1. mouse. [Q91YI6-2]
uc007rla.1. mouse. [Q91YI6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM419011 mRNA. Translation: CAL91354.1 .
BC016629 mRNA. Translation: AAH16629.1 .
AK033141 mRNA. Translation: BAC28170.1 . Frameshift.
CCDSi CCDS26686.1. [Q91YI6-1 ]
RefSeqi NP_598666.1. NM_133905.2. [Q91YI6-1 ]
XP_006517573.1. XM_006517510.1. [Q91YI6-1 ]
XP_006517574.1. XM_006517511.1. [Q91YI6-2 ]
UniGenei Mm.242865.

3D structure databases

ProteinModelPortali Q91YI6.
SMRi Q91YI6. Positions 162-469.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 221516. 2 interactions.

PTM databases

PhosphoSitei Q91YI6.

Proteomic databases

MaxQBi Q91YI6.
PRIDEi Q91YI6.

Protocols and materials databases

DNASUi 100715.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000048702 ; ENSMUSP00000048124 ; ENSMUSG00000042167 . [Q91YI6-1 ]
GeneIDi 100715.
KEGGi mmu:100715.
UCSCi uc007rkz.1. mouse. [Q91YI6-2 ]
uc007rla.1. mouse. [Q91YI6-1 ]

Organism-specific databases

CTDi 167153.
MGIi MGI:2140950. Papd4.

Phylogenomic databases

eggNOGi COG5260.
GeneTreei ENSGT00550000074490.
HOGENOMi HOG000088606.
HOVERGENi HBG097533.
InParanoidi Q91YI6.
KOi K14079.
OMAi CVEFDLN.
OrthoDBi EOG7DRJ2S.
PhylomeDBi Q91YI6.
TreeFami TF315661.

Miscellaneous databases

ChiTaRSi Papd4. mouse.
NextBioi 354606.
PROi Q91YI6.
SOURCEi Search...

Gene expression databases

Bgeei Q91YI6.
Genevestigatori Q91YI6.

Family and domain databases

InterProi IPR002058. PAP_assoc.
[Graphical view ]
Pfami PF03828. PAP_assoc. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "XRbm9, a new XGld2-interacting protein, enhances translation in Xenopus oocytes."
    Papin C.
    Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: NIH Swiss.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 147-484.
    Strain: C57BL/6J.
    Tissue: Testis.
  4. Cited for: ENZYME ACTIVITY.
  5. "Vertebrate GLD2 poly(A) polymerases in the germline and the brain."
    Rouhana L., Wang L., Buter N., Kwak J.E., Schiltz C.A., Gonzalez T., Kelley A.E., Landry C.F., Wickens M.
    RNA 11:1117-1130(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Possible role of mouse poly(A) polymerase mGLD-2 during oocyte maturation."
    Nakanishi T., Kubota H., Ishibashi N., Kumagai S., Watanabe H., Yamashita M., Kashiwabara S., Miyado K., Baba T.
    Dev. Biol. 289:115-126(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF ASP-215.
  7. "Disruption of mouse poly(A) polymerase mGLD-2 does not alter polyadenylation status in oocytes and somatic cells."
    Nakanishi T., Kumagai S., Kimura M., Watanabe H., Sakurai T., Kimura M., Kashiwabara S., Baba T.
    Biochem. Biophys. Res. Commun. 364:14-19(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, INTERACTION WITH CPEB1; CPEB2; CPSF1 AND PABPC1.

Entry informationi

Entry nameiGLD2_MOUSE
AccessioniPrimary (citable) accession number: Q91YI6
Secondary accession number(s): A4F5H0, Q8CCH5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: December 1, 2001
Last modified: November 26, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3