Q91YE9 (5NT1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic 5'-nucleotidase 1B
Short name=cN1B
EC=3.1.3.5

Alternative name(s):
Autoimmune infertility-related protein
Cytosolic 5'-nucleotidase IB
Short name=cN-IB

Gene names

Name:	Nt5c1b
Synonyms:	Airp

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides. Helps to regulate adenosine levels. Ref.1
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Cofactor	Magnesium.
Enzyme regulation	Activated by ADP.
Subcellular location	Cytoplasm.
Tissue specificity	Highly expressed in testis and brain. Detected at lower levels in skeletal muscle, heart and kidney. Ref.1
Sequence similarities	Belongs to the 5'-nucleotidase type 3 family.
Sequence caution	The sequence AAK39109.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleoside metabolic process Inferred from direct assay Ref.1. Source: MGI nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from direct assay Ref.1. Source: MGI
Molecular_function	5'-nucleotidase activity Inferred from direct assay Ref.1. Source: MGI magnesium ion binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q91YE9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q91YE9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     88-89: Missing.
     247-262: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 573	573	Cytosolic 5'-nucleotidase 1B		PRO_0000144797
Sites
	Active site	428	1	Nucleophile By similarity
Natural variations
	Alternative sequence	88 – 89	2	Missing in isoform 2.		VSP_010204
	Alternative sequence	247 – 262	16	Missing in isoform 2.		VSP_010205
Experimental info
	Sequence conflict	103	1	S → F in AAK39109. Ref.2
	Sequence conflict	121	1	I → T in CAC44364. Ref.1
	Sequence conflict	121	1	I → T in AAK39109. Ref.2
	Sequence conflict	162	1	A → V in CAC44364. Ref.1
	Sequence conflict	185	1	E → Q in CAC44364. Ref.1
	Sequence conflict	228	1	Q → H in CAC44364. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 28, 2011. Version 2. Checksum: 948868F505D6B965	FASTA	573	65,002
	10 20 30 40 50 60 MSQTSLKHKK KNEPGMRYSK ESLDAEKRKD SDKTGARLST QGSQELPLHN TDSRGYVVRN 70 80 90 100 110 120 QWSRTSRSPS TGAPSVDEPR SRNTAIKVEA PNSSTTSRTS SASPSQHETS PPPQTSEKSS 130 140 150 160 170 180 IQQTPQNRPI TQLESQPPTP PETEPNSRRT SAKMYTGSDP WAHRENREPR DLQLRDYAYS 190 200 210 220 230 240 CDSREGMPKT REYPRTPPTE WKPYAQRRLQ YGTSVDMEPE YISDGPQQRQ RQQTEEDEVD 250 260 270 280 290 300 EAYWTSVSML YEKIPSCARP RPPKPKHAIT IAVSSRALFN MVDDRKIYEE EGLEKYMEYQ 310 320 330 340 350 360 LTNENVILTP GPAFRFVKAL QHVNSRLRDL YPDEQDLFDI VLMTNNHAQV GVRLINSVNH 370 380 390 400 410 420 YGLLIDRFCL TGGKSPIGYL KAYLTNLYLS ADSEKVQEAI KEGIASATMY AGAKDMAYCD 430 440 450 460 470 480 TQLRVAFDGD AVLFSDESEH IAKDHGLDKF FQHETLFENK PLAQGPLKSF LEDLGKLQKK 490 500 510 520 530 540 FYAKDERLLC PIRTYLVTAR SAASSGARVL KTLRRWGLEI DEALFLAGAP KGPILVKIRP 550 560 570 HIFFDDQMFH IESAQKFGTI TAHVPYGIAQ KRN
	Isoform 2 [UniParc]. Checksum: 99417F747816E36C	FASTA	555	62,945
	10 20 30 40 50 60 MSQTSLKHKK KNEPGMRYSK ESLDAEKRKD SDKTGARLST QGSQELPLHN TDSRGYVVRN 70 80 90 100 110 120 QWSRTSRSPS TGAPSVDEPR SRNTAIKAPN SSTTSRTSSA SPSQHETSPP PQTSEKSSIQ 130 140 150 160 170 180 QTPQNRPITQ LESQPPTPPE TEPNSRRTSA KMYTGSDPWA HRENREPRDL QLRDYAYSCD 190 200 210 220 230 240 SREGMPKTRE YPRTPPTEWK PYAQRRLQYG TSVDMEPEYI SDGPQQRQRQ QTEEDEVDEA 250 260 270 280 290 300 YWTSPKPKHA ITIAVSSRAL FNMVDDRKIY EEEGLEKYME YQLTNENVIL TPGPAFRFVK 310 320 330 340 350 360 ALQHVNSRLR DLYPDEQDLF DIVLMTNNHA QVGVRLINSV NHYGLLIDRF CLTGGKSPIG 370 380 390 400 410 420 YLKAYLTNLY LSADSEKVQE AIKEGIASAT MYAGAKDMAY CDTQLRVAFD GDAVLFSDES 430 440 450 460 470 480 EHIAKDHGLD KFFQHETLFE NKPLAQGPLK SFLEDLGKLQ KKFYAKDERL LCPIRTYLVT 490 500 510 520 530 540 ARSAASSGAR VLKTLRRWGL EIDEALFLAG APKGPILVKI RPHIFFDDQM FHIESAQKFG 550 TITAHVPYGI AQKRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a mouse cytosolic 5'-nucleotidase-I identifies a new gene related to human autoimmune infertility-related protein." Sala-Newby G.B., Newby A.C. Biochim. Biophys. Acta 1521:12-18(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY. Tissue: Testis.
[2]	"Isolation of a novel human testis cDNA encoding a polypeptide related to autoimmune infertility." Setiady Y.Y., Pujianto D.A., Ekowati A.L., Harahap A., Marzuki S. Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: CD-1. Tissue: Testis.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ295253 mRNA. Translation: CAC44364.1. AF356186 mRNA. Translation: AAK39109.1. Different initiation. AC157570 Genomic DNA. No translation available.
IPI	IPI00406365. IPI00410846.
RefSeq	NP_081864.2. NM_027588.3.
UniGene	Mm.272843.
3D structure databases
ProteinModelPortal	Q91YE9.
ModBase	Search...
PTM databases
PhosphoSite	Q91YE9.
Proteomic databases
PRIDE	Q91YE9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000002456; ENSMUSP00000002456; ENSMUSG00000020622. ENSMUST00000118657; ENSMUSP00000112694; ENSMUSG00000020622.
GeneID	70881.
KEGG	mmu:70881.
UCSC	uc007nap.2. mouse.
Organism-specific databases
CTD	93034.
MGI	MGI:1918131. Nt5c1b.
Phylogenomic databases
eggNOG	NOG46880.
GeneTree	ENSGT00390000017767.
HOGENOM	HOG000243802.
HOVERGEN	HBG050426.
InParanoid	Q91YE9.
KO	K01081.
OMA	PKHAITI.
OrthoDB	EOG44XJGN.
Enzyme and pathway databases
BRENDA	3.1.3.5. 244.
SABIO-RK	Q91YE9.
Gene expression databases
ArrayExpress	Q91YE9.
Bgee	Q91YE9.
CleanEx	MM_NT5C1B.
Genevestigator	Q91YE9.
GermOnline	ENSMUSG00000020622. Mus musculus.
Family and domain databases
InterPro	IPR010394. 5-nucleotidase. [Graphical view]
Pfam	PF06189. 5-nucleotidase. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NT5C1B. mouse.
NextBio	332486.
SOURCE	Search...

Entry information

Entry name

5NT1B_MOUSE

Accession

Primary (citable) accession number: Q91YE9
Secondary accession number(s): E9QNH2, Q91Y48

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	June 28, 2011
Last modified:	May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program