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Protein

Cytosolic 5'-nucleotidase 1B

Gene

Nt5c1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides. Helps to regulate adenosine levels.1 Publication

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Enzyme regulationi

Activated by ADP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei428 – 4281NucleophileBy similarity

GO - Molecular functioni

  1. 5'-nucleotidase activity Source: MGI
  2. magnesium ion binding Source: InterPro
  3. nucleotide binding Source: InterPro

GO - Biological processi

  1. adenosine metabolic process Source: Ensembl
  2. dephosphorylation Source: GOC
  3. nucleoside metabolic process Source: MGI
  4. nucleotide metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

ReactomeiREACT_277717. Purine catabolism.
SABIO-RKQ91YE9.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic 5'-nucleotidase 1B (EC:3.1.3.5)
Short name:
cN1B
Alternative name(s):
Autoimmune infertility-related protein
Cytosolic 5'-nucleotidase IB
Short name:
cN-IB
Gene namesi
Name:Nt5c1b
Synonyms:Airp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1918131. Nt5c1b.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 573573Cytosolic 5'-nucleotidase 1BPRO_0000144797Add
BLAST

Proteomic databases

PRIDEiQ91YE9.

PTM databases

PhosphoSiteiQ91YE9.

Expressioni

Tissue specificityi

Highly expressed in testis and brain. Detected at lower levels in skeletal muscle, heart and kidney.1 Publication

Gene expression databases

BgeeiQ91YE9.
CleanExiMM_NT5C1B.
ExpressionAtlasiQ91YE9. baseline.
GenevestigatoriQ91YE9.

Structurei

3D structure databases

ProteinModelPortaliQ91YE9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 5'-nucleotidase type 3 family.Curated

Phylogenomic databases

eggNOGiNOG46880.
GeneTreeiENSGT00390000017767.
HOGENOMiHOG000243802.
HOVERGENiHBG050426.
InParanoidiQ91YE9.
KOiK01081.
OMAiPKPKHAI.
OrthoDBiEOG79KPF1.
TreeFamiTF329831.

Family and domain databases

InterProiIPR010394. 5-nucleotidase.
[Graphical view]
PfamiPF06189. 5-nucleotidase. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91YE9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQTSLKHKK KNEPGMRYSK ESLDAEKRKD SDKTGARLST QGSQELPLHN
60 70 80 90 100
TDSRGYVVRN QWSRTSRSPS TGAPSVDEPR SRNTAIKVEA PNSSTTSRTS
110 120 130 140 150
SASPSQHETS PPPQTSEKSS IQQTPQNRPI TQLESQPPTP PETEPNSRRT
160 170 180 190 200
SAKMYTGSDP WAHRENREPR DLQLRDYAYS CDSREGMPKT REYPRTPPTE
210 220 230 240 250
WKPYAQRRLQ YGTSVDMEPE YISDGPQQRQ RQQTEEDEVD EAYWTSVSML
260 270 280 290 300
YEKIPSCARP RPPKPKHAIT IAVSSRALFN MVDDRKIYEE EGLEKYMEYQ
310 320 330 340 350
LTNENVILTP GPAFRFVKAL QHVNSRLRDL YPDEQDLFDI VLMTNNHAQV
360 370 380 390 400
GVRLINSVNH YGLLIDRFCL TGGKSPIGYL KAYLTNLYLS ADSEKVQEAI
410 420 430 440 450
KEGIASATMY AGAKDMAYCD TQLRVAFDGD AVLFSDESEH IAKDHGLDKF
460 470 480 490 500
FQHETLFENK PLAQGPLKSF LEDLGKLQKK FYAKDERLLC PIRTYLVTAR
510 520 530 540 550
SAASSGARVL KTLRRWGLEI DEALFLAGAP KGPILVKIRP HIFFDDQMFH
560 570
IESAQKFGTI TAHVPYGIAQ KRN
Length:573
Mass (Da):65,002
Last modified:June 28, 2011 - v2
Checksum:i948868F505D6B965
GO
Isoform 2 (identifier: Q91YE9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     88-89: Missing.
     247-262: Missing.

Note: No experimental confirmation available.

Show »
Length:555
Mass (Da):62,945
Checksum:i99417F747816E36C
GO

Sequence cautioni

The sequence AAK39109.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti103 – 1031S → F in AAK39109 (Ref. 2) Curated
Sequence conflicti121 – 1211I → T in CAC44364 (PubMed:11690631).Curated
Sequence conflicti121 – 1211I → T in AAK39109 (Ref. 2) Curated
Sequence conflicti162 – 1621A → V in CAC44364 (PubMed:11690631).Curated
Sequence conflicti185 – 1851E → Q in CAC44364 (PubMed:11690631).Curated
Sequence conflicti228 – 2281Q → H in CAC44364 (PubMed:11690631).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei88 – 892Missing in isoform 2. 1 PublicationVSP_010204
Alternative sequencei247 – 26216Missing in isoform 2. 1 PublicationVSP_010205Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ295253 mRNA. Translation: CAC44364.1.
AF356186 mRNA. Translation: AAK39109.1. Different initiation.
AC157570 Genomic DNA. No translation available.
CCDSiCCDS25810.1. [Q91YE9-1]
RefSeqiNP_081864.2. NM_027588.3. [Q91YE9-1]
UniGeneiMm.272843.

Genome annotation databases

EnsembliENSMUST00000002456; ENSMUSP00000002456; ENSMUSG00000020622. [Q91YE9-1]
ENSMUST00000118657; ENSMUSP00000112694; ENSMUSG00000020622. [Q91YE9-2]
GeneIDi70881.
KEGGimmu:70881.
UCSCiuc007nap.2. mouse. [Q91YE9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ295253 mRNA. Translation: CAC44364.1.
AF356186 mRNA. Translation: AAK39109.1. Different initiation.
AC157570 Genomic DNA. No translation available.
CCDSiCCDS25810.1. [Q91YE9-1]
RefSeqiNP_081864.2. NM_027588.3. [Q91YE9-1]
UniGeneiMm.272843.

3D structure databases

ProteinModelPortaliQ91YE9.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ91YE9.

Proteomic databases

PRIDEiQ91YE9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002456; ENSMUSP00000002456; ENSMUSG00000020622. [Q91YE9-1]
ENSMUST00000118657; ENSMUSP00000112694; ENSMUSG00000020622. [Q91YE9-2]
GeneIDi70881.
KEGGimmu:70881.
UCSCiuc007nap.2. mouse. [Q91YE9-1]

Organism-specific databases

CTDi93034.
MGIiMGI:1918131. Nt5c1b.

Phylogenomic databases

eggNOGiNOG46880.
GeneTreeiENSGT00390000017767.
HOGENOMiHOG000243802.
HOVERGENiHBG050426.
InParanoidiQ91YE9.
KOiK01081.
OMAiPKPKHAI.
OrthoDBiEOG79KPF1.
TreeFamiTF329831.

Enzyme and pathway databases

ReactomeiREACT_277717. Purine catabolism.
SABIO-RKQ91YE9.

Miscellaneous databases

ChiTaRSiNt5c1b. mouse.
NextBioi332486.
PROiQ91YE9.
SOURCEiSearch...

Gene expression databases

BgeeiQ91YE9.
CleanExiMM_NT5C1B.
ExpressionAtlasiQ91YE9. baseline.
GenevestigatoriQ91YE9.

Family and domain databases

InterProiIPR010394. 5-nucleotidase.
[Graphical view]
PfamiPF06189. 5-nucleotidase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a mouse cytosolic 5'-nucleotidase-I identifies a new gene related to human autoimmune infertility-related protein."
    Sala-Newby G.B., Newby A.C.
    Biochim. Biophys. Acta 1521:12-18(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "Isolation of a novel human testis cDNA encoding a polypeptide related to autoimmune infertility."
    Setiady Y.Y., Pujianto D.A., Ekowati A.L., Harahap A., Marzuki S.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: CD-1.
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry namei5NT1B_MOUSE
AccessioniPrimary (citable) accession number: Q91YE9
Secondary accession number(s): E9QNH2, Q91Y48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 28, 2011
Last modified: April 1, 2015
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.