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Q91YE9

- 5NT1B_MOUSE

UniProt

Q91YE9 - 5NT1B_MOUSE

Protein

Cytosolic 5'-nucleotidase 1B

Gene

Nt5c1b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides. Helps to regulate adenosine levels.1 Publication

    Catalytic activityi

    A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

    Cofactori

    Magnesium.

    Enzyme regulationi

    Activated by ADP.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei428 – 4281NucleophileBy similarity

    GO - Molecular functioni

    1. 5'-nucleotidase activity Source: MGI
    2. magnesium ion binding Source: InterPro
    3. nucleotide binding Source: InterPro

    GO - Biological processi

    1. adenosine metabolic process Source: Ensembl
    2. dephosphorylation Source: GOC
    3. nucleoside metabolic process Source: MGI
    4. nucleotide metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Nucleotide metabolism

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BRENDAi3.1.3.5. 244.
    SABIO-RKQ91YE9.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosolic 5'-nucleotidase 1B (EC:3.1.3.5)
    Short name:
    cN1B
    Alternative name(s):
    Autoimmune infertility-related protein
    Cytosolic 5'-nucleotidase IB
    Short name:
    cN-IB
    Gene namesi
    Name:Nt5c1b
    Synonyms:Airp
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:1918131. Nt5c1b.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: MGI

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 573573Cytosolic 5'-nucleotidase 1BPRO_0000144797Add
    BLAST

    Proteomic databases

    PRIDEiQ91YE9.

    PTM databases

    PhosphoSiteiQ91YE9.

    Expressioni

    Tissue specificityi

    Highly expressed in testis and brain. Detected at lower levels in skeletal muscle, heart and kidney.1 Publication

    Gene expression databases

    BgeeiQ91YE9.
    CleanExiMM_NT5C1B.
    GenevestigatoriQ91YE9.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91YE9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the 5'-nucleotidase type 3 family.Curated

    Phylogenomic databases

    eggNOGiNOG46880.
    GeneTreeiENSGT00390000017767.
    HOGENOMiHOG000243802.
    HOVERGENiHBG050426.
    InParanoidiQ91YE9.
    KOiK01081.
    OMAiPKHAITI.
    OrthoDBiEOG79KPF1.
    TreeFamiTF329831.

    Family and domain databases

    InterProiIPR010394. 5-nucleotidase.
    [Graphical view]
    PfamiPF06189. 5-nucleotidase. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q91YE9-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSQTSLKHKK KNEPGMRYSK ESLDAEKRKD SDKTGARLST QGSQELPLHN    50
    TDSRGYVVRN QWSRTSRSPS TGAPSVDEPR SRNTAIKVEA PNSSTTSRTS 100
    SASPSQHETS PPPQTSEKSS IQQTPQNRPI TQLESQPPTP PETEPNSRRT 150
    SAKMYTGSDP WAHRENREPR DLQLRDYAYS CDSREGMPKT REYPRTPPTE 200
    WKPYAQRRLQ YGTSVDMEPE YISDGPQQRQ RQQTEEDEVD EAYWTSVSML 250
    YEKIPSCARP RPPKPKHAIT IAVSSRALFN MVDDRKIYEE EGLEKYMEYQ 300
    LTNENVILTP GPAFRFVKAL QHVNSRLRDL YPDEQDLFDI VLMTNNHAQV 350
    GVRLINSVNH YGLLIDRFCL TGGKSPIGYL KAYLTNLYLS ADSEKVQEAI 400
    KEGIASATMY AGAKDMAYCD TQLRVAFDGD AVLFSDESEH IAKDHGLDKF 450
    FQHETLFENK PLAQGPLKSF LEDLGKLQKK FYAKDERLLC PIRTYLVTAR 500
    SAASSGARVL KTLRRWGLEI DEALFLAGAP KGPILVKIRP HIFFDDQMFH 550
    IESAQKFGTI TAHVPYGIAQ KRN 573
    Length:573
    Mass (Da):65,002
    Last modified:June 28, 2011 - v2
    Checksum:i948868F505D6B965
    GO
    Isoform 2 (identifier: Q91YE9-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         88-89: Missing.
         247-262: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:555
    Mass (Da):62,945
    Checksum:i99417F747816E36C
    GO

    Sequence cautioni

    The sequence AAK39109.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti103 – 1031S → F in AAK39109. 1 PublicationCurated
    Sequence conflicti121 – 1211I → T in CAC44364. (PubMed:11690631)Curated
    Sequence conflicti121 – 1211I → T in AAK39109. 1 PublicationCurated
    Sequence conflicti162 – 1621A → V in CAC44364. (PubMed:11690631)Curated
    Sequence conflicti185 – 1851E → Q in CAC44364. (PubMed:11690631)Curated
    Sequence conflicti228 – 2281Q → H in CAC44364. (PubMed:11690631)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei88 – 892Missing in isoform 2. 1 PublicationVSP_010204
    Alternative sequencei247 – 26216Missing in isoform 2. 1 PublicationVSP_010205Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ295253 mRNA. Translation: CAC44364.1.
    AF356186 mRNA. Translation: AAK39109.1. Different initiation.
    AC157570 Genomic DNA. No translation available.
    CCDSiCCDS25810.1. [Q91YE9-1]
    RefSeqiNP_081864.2. NM_027588.3. [Q91YE9-1]
    UniGeneiMm.272843.

    Genome annotation databases

    EnsembliENSMUST00000002456; ENSMUSP00000002456; ENSMUSG00000020622. [Q91YE9-1]
    ENSMUST00000118657; ENSMUSP00000112694; ENSMUSG00000020622. [Q91YE9-2]
    GeneIDi70881.
    KEGGimmu:70881.
    UCSCiuc007nap.2. mouse. [Q91YE9-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ295253 mRNA. Translation: CAC44364.1 .
    AF356186 mRNA. Translation: AAK39109.1 . Different initiation.
    AC157570 Genomic DNA. No translation available.
    CCDSi CCDS25810.1. [Q91YE9-1 ]
    RefSeqi NP_081864.2. NM_027588.3. [Q91YE9-1 ]
    UniGenei Mm.272843.

    3D structure databases

    ProteinModelPortali Q91YE9.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q91YE9.

    Proteomic databases

    PRIDEi Q91YE9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002456 ; ENSMUSP00000002456 ; ENSMUSG00000020622 . [Q91YE9-1 ]
    ENSMUST00000118657 ; ENSMUSP00000112694 ; ENSMUSG00000020622 . [Q91YE9-2 ]
    GeneIDi 70881.
    KEGGi mmu:70881.
    UCSCi uc007nap.2. mouse. [Q91YE9-1 ]

    Organism-specific databases

    CTDi 93034.
    MGIi MGI:1918131. Nt5c1b.

    Phylogenomic databases

    eggNOGi NOG46880.
    GeneTreei ENSGT00390000017767.
    HOGENOMi HOG000243802.
    HOVERGENi HBG050426.
    InParanoidi Q91YE9.
    KOi K01081.
    OMAi PKHAITI.
    OrthoDBi EOG79KPF1.
    TreeFami TF329831.

    Enzyme and pathway databases

    BRENDAi 3.1.3.5. 244.
    SABIO-RK Q91YE9.

    Miscellaneous databases

    ChiTaRSi NT5C1B. mouse.
    NextBioi 332486.
    PROi Q91YE9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q91YE9.
    CleanExi MM_NT5C1B.
    Genevestigatori Q91YE9.

    Family and domain databases

    InterProi IPR010394. 5-nucleotidase.
    [Graphical view ]
    Pfami PF06189. 5-nucleotidase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a mouse cytosolic 5'-nucleotidase-I identifies a new gene related to human autoimmune infertility-related protein."
      Sala-Newby G.B., Newby A.C.
      Biochim. Biophys. Acta 1521:12-18(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
      Tissue: Testis.
    2. "Isolation of a novel human testis cDNA encoding a polypeptide related to autoimmune infertility."
      Setiady Y.Y., Pujianto D.A., Ekowati A.L., Harahap A., Marzuki S.
      Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: CD-1.
      Tissue: Testis.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry namei5NT1B_MOUSE
    AccessioniPrimary (citable) accession number: Q91YE9
    Secondary accession number(s): E9QNH2, Q91Y48
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2004
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3