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Protein

RNA-binding protein 5

Gene

Rbm5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes production of a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes production of a catalytically active form of CASP2/Caspase-2 that induces apoptosis (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri181 – 21030RanBP2-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri647 – 67226C2H2-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein 5
Alternative name(s):
Putative tumor suppressor LUCA15
RNA-binding motif protein 5
Gene namesi
Name:Rbm5
Synonyms:Luca15
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1933204. Rbm5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 815815RNA-binding protein 5PRO_0000253050Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei18 – 181PhosphoserineBy similarity
Modified residuei59 – 591PhosphoserineBy similarity
Modified residuei69 – 691PhosphoserineBy similarity
Modified residuei72 – 721PhosphoserineBy similarity
Modified residuei78 – 781PhosphoserineCombined sources
Modified residuei444 – 4441PhosphoserineBy similarity
Modified residuei621 – 6211PhosphoserineCombined sources
Modified residuei624 – 6241PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ91YE7.
PaxDbiQ91YE7.
PeptideAtlasiQ91YE7.
PRIDEiQ91YE7.

PTM databases

iPTMnetiQ91YE7.
PhosphoSiteiQ91YE7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032580.
CleanExiMM_RBM5.
ExpressionAtlasiQ91YE7. baseline and differential.
GenevisibleiQ91YE7. MM.

Interactioni

Subunit structurei

Component of the spliceosome A complex (also known as the prespliceosome). Appears to dissociate from the spliceosome upon formation of the spliceosome B complex (also known as the precatalytic spliceosome), in which the heterotrimeric U4/U6.U5 snRNPs are bound. Interacts with U2AF2; this interaction is direct. Also interacts with ACIN1, PRPF8, SFRS3, SNRPB, SNRPN, SNRNP70 and SNRNP200; these interactions may be indirect (By similarity).By similarity

Protein-protein interaction databases

BioGridi219933. 2 interactions.
IntActiQ91YE7. 1 interaction.
MINTiMINT-4121863.
STRINGi10090.ENSMUSP00000035199.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CLVX-ray1.90C/M484-491[»]
2OL3X-ray2.90P484-491[»]
ProteinModelPortaliQ91YE7.
SMRiQ91YE7. Positions 97-210, 231-316, 452-521.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91YE7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini98 – 17881RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini231 – 31585RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini743 – 78947G-patchPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni321 – 809489Required for interaction with U2AF2By similarityAdd
BLAST
Regioni452 – 53584Sufficient for interaction with ACIN1, PRPF8, SFRS3, SNRPB, SNRPN, SNRNP70 and SNRNP200By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi6 – 7166Arg-richAdd
BLAST
Compositional biasi462 – 49736Tyr-richAdd
BLAST

Sequence similaritiesi

Belongs to the RBM5/RBM10 family.Curated
Contains 1 C2H2-type zinc finger.PROSITE-ProRule annotation
Contains 1 G-patch domain.PROSITE-ProRule annotation
Contains 1 RanBP2-type zinc finger.PROSITE-ProRule annotation
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri181 – 21030RanBP2-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri647 – 67226C2H2-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG0154. Eukaryota.
ENOG410YNFQ. LUCA.
GeneTreeiENSGT00510000046476.
HOGENOMiHOG000046285.
HOVERGENiHBG000318.
InParanoidiQ91YE7.
KOiK13094.
OMAiDCRDYDS.
OrthoDBiEOG091G01UT.
PhylomeDBiQ91YE7.
TreeFamiTF315789.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
4.10.1060.10. 1 hit.
InterProiIPR000467. G_patch_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR033094. RBM5.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR001876. Znf_RanBP2.
[Graphical view]
PANTHERiPTHR13948:SF21. PTHR13948:SF21. 2 hits.
PfamiPF01585. G-patch. 1 hit.
PF00076. RRM_1. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTiSM00443. G_patch. 1 hit.
SM00360. RRM. 2 hits.
SM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
SSF90209. SSF90209. 1 hit.
PROSITEiPS50174. G_PATCH. 1 hit.
PS50102. RRM. 2 hits.
PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q91YE7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSDKRVSRT ERSGRYGSII DRDDRDERES RSRRRDSDYK RSSDDRRGDR
60 70 80 90 100
YDDYRDYDSP ERERERRNSD RSEDGYHSDG DYGEHDYRHD ISDERESKTI
110 120 130 140 150
MLRGLPITIT ESDIREMMES FEGPQPADVR LMKRKTGVSR GFAFVEFYHL
160 170 180 190 200
QDATSWMEAN QKKLVIQGKH IAMHYSNPRP KFEDWLCNKC CLNNFRKRLK
210 220 230 240 250
CFRCGADKFD SEQEVPPGTT ESAQSVDYYC DTIILRNIAP HTVVDSIMTA
260 270 280 290 300
LSPYASLAVN NIRLIKDKQT QQNRGFAFVQ LSSAMDASQL LQILQSLHPP
310 320 330 340 350
LKIDGKTIGV DFAKSARKDL VLPDGNRVSA FSVASTAIAA AQWSSTQSQS
360 370 380 390 400
GEGGSVDYSY MQPGQDGYTQ YTQYSQDYQQ FYQQQAGGLE SDTSATSGTT
410 420 430 440 450
VTTTSAAVVS QSPQLYNQTS NPPGSPTEEA QPSTSTSTQA PAASPTGVVP
460 470 480 490 500
GTKYAVPDTS TYQYDESSGY YYDPTTGLYY DPNSQYYYNS LTQQYLYWDG
510 520 530 540 550
EKETYVPAAE ASSNQQTGLP STKEGKEKKE KPKSKTAQQI AKDMERWAKS
560 570 580 590 600
LNKQKENFKN SFQPVNSLRE EERRESAAAD AGFALFEKKG ALAERQQLLP
610 620 630 640 650
ELVRNGDEEN PLKRGLVAAY SGDSDNEEEL VERLESEEEK LADWKKMACL
660 670 680 690 700
LCRRQFPNRD ALVRHQQLSD LHKQNMDIYR RSRLSEQELE ALELREREMK
710 720 730 740 750
YRDRAAERRE KYGIPEPPEP KRKKQFDAGT VNYEQPTKDG IDHSNIGNKM
760 770 780 790 800
LQAMGWREGS GLGRKCQGIT APIEAQVRLK GAGLGAKGSA YGLSGADSYK
810
DAVRKAMFAR FTEME
Length:815
Mass (Da):92,311
Last modified:December 1, 2001 - v1
Checksum:iBCDADD4A8FB4EAC9
GO
Isoform 2 (identifier: Q91YE7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     455-455: Missing.

Show »
Length:814
Mass (Da):92,240
Checksum:i86BD2751A6E3E1B3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti272 – 2721Q → K in BAE22042 (PubMed:16141072).Curated
Sequence conflicti693 – 6931E → G in BAE22042 (PubMed:16141072).Curated
Sequence conflicti726 – 7261F → L in BAE22042 (PubMed:16141072).Curated
Sequence conflicti798 – 7981S → P in BAE22042 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei455 – 4551Missing in isoform 2. 1 PublicationVSP_021003

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ309168 mRNA. Translation: CAC69136.1.
AK134183 mRNA. Translation: BAE22042.1.
AK146880 mRNA. Translation: BAE27501.1.
BC003988 mRNA. Translation: AAH03988.1.
BC023854 mRNA. Translation: AAH23854.1.
BC031899 mRNA. Translation: AAH31899.1.
BC043058 mRNA. Translation: AAH43058.1.
BC054729 mRNA. Translation: AAH54729.1.
CCDSiCCDS23506.1. [Q91YE7-1]
RefSeqiNP_683732.1. NM_148930.3. [Q91YE7-1]
XP_006511933.1. XM_006511870.2. [Q91YE7-1]
XP_006511934.1. XM_006511871.2. [Q91YE7-1]
XP_006511935.1. XM_006511872.2. [Q91YE7-1]
XP_011241223.1. XM_011242921.1. [Q91YE7-2]
UniGeneiMm.259197.

Genome annotation databases

EnsembliENSMUST00000035199; ENSMUSP00000035199; ENSMUSG00000032580. [Q91YE7-2]
ENSMUST00000182659; ENSMUSP00000138379; ENSMUSG00000032580. [Q91YE7-1]
GeneIDi83486.
KEGGimmu:83486.
UCSCiuc009rmv.2. mouse. [Q91YE7-2]
uc009rmw.2. mouse. [Q91YE7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ309168 mRNA. Translation: CAC69136.1.
AK134183 mRNA. Translation: BAE22042.1.
AK146880 mRNA. Translation: BAE27501.1.
BC003988 mRNA. Translation: AAH03988.1.
BC023854 mRNA. Translation: AAH23854.1.
BC031899 mRNA. Translation: AAH31899.1.
BC043058 mRNA. Translation: AAH43058.1.
BC054729 mRNA. Translation: AAH54729.1.
CCDSiCCDS23506.1. [Q91YE7-1]
RefSeqiNP_683732.1. NM_148930.3. [Q91YE7-1]
XP_006511933.1. XM_006511870.2. [Q91YE7-1]
XP_006511934.1. XM_006511871.2. [Q91YE7-1]
XP_006511935.1. XM_006511872.2. [Q91YE7-1]
XP_011241223.1. XM_011242921.1. [Q91YE7-2]
UniGeneiMm.259197.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CLVX-ray1.90C/M484-491[»]
2OL3X-ray2.90P484-491[»]
ProteinModelPortaliQ91YE7.
SMRiQ91YE7. Positions 97-210, 231-316, 452-521.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219933. 2 interactions.
IntActiQ91YE7. 1 interaction.
MINTiMINT-4121863.
STRINGi10090.ENSMUSP00000035199.

PTM databases

iPTMnetiQ91YE7.
PhosphoSiteiQ91YE7.

Proteomic databases

MaxQBiQ91YE7.
PaxDbiQ91YE7.
PeptideAtlasiQ91YE7.
PRIDEiQ91YE7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035199; ENSMUSP00000035199; ENSMUSG00000032580. [Q91YE7-2]
ENSMUST00000182659; ENSMUSP00000138379; ENSMUSG00000032580. [Q91YE7-1]
GeneIDi83486.
KEGGimmu:83486.
UCSCiuc009rmv.2. mouse. [Q91YE7-2]
uc009rmw.2. mouse. [Q91YE7-1]

Organism-specific databases

CTDi10181.
MGIiMGI:1933204. Rbm5.

Phylogenomic databases

eggNOGiKOG0154. Eukaryota.
ENOG410YNFQ. LUCA.
GeneTreeiENSGT00510000046476.
HOGENOMiHOG000046285.
HOVERGENiHBG000318.
InParanoidiQ91YE7.
KOiK13094.
OMAiDCRDYDS.
OrthoDBiEOG091G01UT.
PhylomeDBiQ91YE7.
TreeFamiTF315789.

Enzyme and pathway databases

ReactomeiR-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiRbm5. mouse.
EvolutionaryTraceiQ91YE7.
PROiQ91YE7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032580.
CleanExiMM_RBM5.
ExpressionAtlasiQ91YE7. baseline and differential.
GenevisibleiQ91YE7. MM.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
4.10.1060.10. 1 hit.
InterProiIPR000467. G_patch_dom.
IPR012677. Nucleotide-bd_a/b_plait.
IPR033094. RBM5.
IPR000504. RRM_dom.
IPR007087. Znf_C2H2.
IPR001876. Znf_RanBP2.
[Graphical view]
PANTHERiPTHR13948:SF21. PTHR13948:SF21. 2 hits.
PfamiPF01585. G-patch. 1 hit.
PF00076. RRM_1. 1 hit.
PF00641. zf-RanBP. 1 hit.
[Graphical view]
SMARTiSM00443. G_patch. 1 hit.
SM00360. RRM. 2 hits.
SM00547. ZnF_RBZ. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
SSF90209. SSF90209. 1 hit.
PROSITEiPS50174. G_PATCH. 1 hit.
PS50102. RRM. 2 hits.
PS01358. ZF_RANBP2_1. 1 hit.
PS50199. ZF_RANBP2_2. 1 hit.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBM5_MOUSE
AccessioniPrimary (citable) accession number: Q91YE7
Secondary accession number(s): Q3UZ19, Q99KV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: December 1, 2001
Last modified: September 7, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.