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Protein

Importin-9

Gene

Ipo9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS7, RPL18A and H2B histone. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import RPL4, RPL6 H2A, H3 and H4 histones.By similarity1 Publication

GO - Molecular functioni

  • histone binding Source: MGI
  • protein transporter activity Source: UniProtKB
  • Ran GTPase binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Importin-9
Short name:
Imp9
Alternative name(s):
Importin-9a
Short name:
Imp9a
Importin-9b
Short name:
Imp9b
Ran-binding protein 9
Short name:
RanBP9
Gene namesi
Name:Ipo9
Synonyms:Imp9, Ranbp9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1918944. Ipo9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 10411040Importin-9PRO_0000120755Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ91YE6.
MaxQBiQ91YE6.
PaxDbiQ91YE6.
PRIDEiQ91YE6.

PTM databases

iPTMnetiQ91YE6.
PhosphoSiteiQ91YE6.
SwissPalmiQ91YE6.

Expressioni

Gene expression databases

CleanExiMM_IPO9.
MM_RANBP9.

Interactioni

Subunit structurei

Interacts with PPP2R1A and PPP2R1B (By similarity). Binds with high affinity to RPS7, RPL18A and H2B histone. The binding is coupled to RanGTP cycles. May bind RPL4, RPL6, H2A, H3 and H4 histones with low affinity.By similarity2 Publications

GO - Molecular functioni

  • histone binding Source: MGI
  • Ran GTPase binding Source: MGI

Protein-protein interaction databases

DIPiDIP-34268N.
IntActiQ91YE6. 3 interactions.
STRINGi10090.ENSMUSP00000036093.

Structurei

3D structure databases

ProteinModelPortaliQ91YE6.
SMRiQ91YE6. Positions 491-546.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 11977Importin N-terminalPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the importin beta family.Curated
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2274. Eukaryota.
COG5657. LUCA.
HOGENOMiHOG000237316.
HOVERGENiHBG049054.
InParanoidiQ91YE6.
PhylomeDBiQ91YE6.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91YE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAAAAGAA SGLPGPVAQG LKEALVDTLT GILSPVQEVR AAAEEQIKVL
60 70 80 90 100
EVTEEFGVHL AELTVDPQGA LAIRQLASVI LKQYVETHWC AQSEKFRPPE
110 120 130 140 150
TTERAKIVIR ELLPNGLRES ISKVRSSVAY AVSAIAHWDW PEAWPQLFNL
160 170 180 190 200
LMEMLVSGDL NAVHGAMRVL TEFTREVTDT QMPLVAPVIL PEMYKIFTMA
210 220 230 240 250
EVYGIRTRSR AVEIFTTCAH MICNMEELEK GAAKVLIFPV VQQFTEAFVQ
260 270 280 290 300
ALQMPDGPTS DSGFKMEVLK AVTALVKNFP KHMVSSMQQI LPIVWNTLTE
310 320 330 340 350
SAAFYVRTEV NYTEEVEDPV DSDGEVLGFE NLVFSIFEFV HALLENSKFK
360 370 380 390 400
STVKKALPEL IYYIILYMQI TEEQIKVWTA NPQQFVEDED DDTFSYTVRI
410 420 430 440 450
AAQDLLLAVA TDFQNESAVA LATAATRHLQ EAEQTKASGT EHWWKIHEAC
460 470 480 490 500
MLALGSVKSI ITDSVKNGRI HFDMHGFLTN VILADLNLSA SPFLLGRALW
510 520 530 540 550
AASRFTVAMS PELIQQFLQA TVSGLHETQP PSVRISAVRA IWGYCDQLKV
560 570 580 590 600
SESTHVLQPF LPSILDGLIH LAAQFSSEVL NLVMETLCIV CTVDPEFTAS
610 620 630 640 650
VENKICPFTI AIFLKYSNDP VVASLAQDIF KELSQIEACQ GPMQMRLIPT
660 670 680 690 700
LVSIMQAPAD KIPAGLCATA IDILTTVVRN TKPPLSQLLI CQAFPAVAQC
710 720 730 740 750
TLHTDDNATM QNGGECLRAY VSVTLEQVAQ WHDEQGHNGL WYVMQVVSQL
760 770 780 790 800
LDPRTSEFTA AFVGRLVSTL ISKAGRELGE NLDQILRAIL SKMQQAETLS
810 820 830 840 850
VMQSLIMVFA HLVHTQLEPL LEFLCSLPGP TGKPALEFVM AEWTSRQHLF
860 870 880 890 900
YGQYEGKVSS VALCKLLQHG INADDKRLQD IRVKGEEIYS MDEGIRTRSK
910 920 930 940 950
SAKNPERWTN IPLLVKILKL IINELSNVME ANAARQATPA EWNQDDSNDM
960 970 980 990 1000
WEDQEEEEEE EEDGLAGQLL SDILATSKYE EDYYEDDEED DPDALKDPLY
1010 1020 1030 1040
QIDLQAYLTD FLCQFAQQPC YIMFSCHLND NERRVLQTIG I
Length:1,041
Mass (Da):116,052
Last modified:January 23, 2007 - v3
Checksum:i2307E00D0AEABEA4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti166 – 1661A → V in AAK91127 (PubMed:11823430).Curated
Sequence conflicti277 – 2771K → E in AAK91127 (PubMed:11823430).Curated
Sequence conflicti782 – 7821L → P in AAK91127 (PubMed:11823430).Curated
Sequence conflicti793 – 7931M → V in AAK91128 (PubMed:11823430).Curated
Sequence conflicti801 – 8011V → A in AAK91127 (PubMed:11823430).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ309238 mRNA. Translation: CAC69407.1.
AF273672 mRNA. Translation: AAK91127.1.
AF273673 mRNA. Translation: AAK91128.1.
UniGeneiMm.271642.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ309238 mRNA. Translation: CAC69407.1.
AF273672 mRNA. Translation: AAK91127.1.
AF273673 mRNA. Translation: AAK91128.1.
UniGeneiMm.271642.

3D structure databases

ProteinModelPortaliQ91YE6.
SMRiQ91YE6. Positions 491-546.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-34268N.
IntActiQ91YE6. 3 interactions.
STRINGi10090.ENSMUSP00000036093.

PTM databases

iPTMnetiQ91YE6.
PhosphoSiteiQ91YE6.
SwissPalmiQ91YE6.

Proteomic databases

EPDiQ91YE6.
MaxQBiQ91YE6.
PaxDbiQ91YE6.
PRIDEiQ91YE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1918944. Ipo9.

Phylogenomic databases

eggNOGiKOG2274. Eukaryota.
COG5657. LUCA.
HOGENOMiHOG000237316.
HOVERGENiHBG049054.
InParanoidiQ91YE6.
PhylomeDBiQ91YE6.

Miscellaneous databases

ChiTaRSiIpo9. mouse.
PROiQ91YE6.
SOURCEiSearch...

Gene expression databases

CleanExiMM_IPO9.
MM_RANBP9.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Multiple pathways contribute to nuclear import of core histones."
    Muehlhaeusser P., Mueller E.-C., Otto A., Kutay U.
    EMBO Rep. 2:690-696(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH HISTONES H2B; H2A; H3 AND H4.
  2. "Importins fulfill a dual function as nuclear import receptors and cytoplasmic chaperones for exposed basic domains."
    Jaekel S., Mingot J.-M., Schwarzmaier P., Hartmann E., Goerlich D.
    EMBO J. 21:377-386(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH RPS7; RPL18A; RPL4 AND RPL6.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiIPO9_MOUSE
AccessioniPrimary (citable) accession number: Q91YE6
Secondary accession number(s): Q924M3, Q924M4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 2, 2004
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.