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Protein

Neural Wiskott-Aldrich syndrome protein

Gene

Wasl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization (By similarity). Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.By similarity1 Publication

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • actin nucleation Source: MGI
  • cell division Source: UniProtKB-KW
  • cellular protein complex localization Source: MGI
  • ephrin receptor signaling pathway Source: Reactome
  • Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
  • membrane budding Source: BHF-UCL
  • mitotic nuclear division Source: UniProtKB-KW
  • negative regulation of lymphocyte migration Source: Ensembl
  • negative regulation of membrane tubulation Source: Ensembl
  • positive regulation of Arp2/3 complex-mediated actin nucleation Source: InterPro
  • positive regulation of clathrin-dependent endocytosis Source: BHF-UCL
  • positive regulation of filopodium assembly Source: BHF-UCL
  • regulation of protein localization Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to bacterium Source: MGI
  • spindle localization Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • vesicle organization Source: BHF-UCL
  • vesicle transport along actin filament Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-MMU-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-MMU-203641. NOSTRIN mediated eNOS trafficking.
R-MMU-373753. Nephrin interactions.
R-MMU-3928662. EPHB-mediated forward signaling.
R-MMU-418885. DCC mediated attractive signaling.
R-MMU-5663213. RHO GTPases Activate WASPs and WAVEs.
R-MMU-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Neural Wiskott-Aldrich syndrome protein
Short name:
N-WASP
Gene namesi
Name:Wasl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1920428. Wasl.

Subcellular locationi

GO - Cellular componenti

  • actin cap Source: MGI
  • cytoplasm Source: MGI
  • cytoplasmic, membrane-bounded vesicle Source: BHF-UCL
  • cytosol Source: Reactome
  • extracellular exosome Source: Ensembl
  • lamellipodium Source: MGI
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi253Y → E: No effect on phosphorylation. Protein preferentially localized in cytoplasm. 1 Publication1
Mutagenesisi253Y → F: Abolishes phosphorylation. Protein preferentially localized in nucleus. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001890012 – 501Neural Wiskott-Aldrich syndrome proteinAdd BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei239Phosphoserine; by TNK2By similarity1
Modified residuei253Phosphotyrosine; by FAK1 and TNK2Combined sources1 Publication1
Modified residuei304Omega-N-methylarginineBy similarity1
Modified residuei480PhosphoserineCombined sources1
Modified residuei481PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

MaxQBiQ91YD9.
PaxDbiQ91YD9.
PRIDEiQ91YD9.

PTM databases

iPTMnetiQ91YD9.
PhosphoSitePlusiQ91YD9.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029684.
CleanExiMM_WASL.
ExpressionAtlasiQ91YD9. baseline and differential.
GenevisibleiQ91YD9. MM.

Interactioni

Subunit structurei

Interacts with NOSTRIN, SNX9 and SNX33 (By similarity). Binds to TNK2 (By similarity). Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11. Interacts with PTK2/FAK1. Interacts with PACSIN1, PACSIN2 and PACSIN3.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CttnQ605984EBI-642417,EBI-397955
Itgb1P090552EBI-642417,EBI-644224
PdgfrbP056222EBI-642417,EBI-1554855

Protein-protein interaction databases

BioGridi215819. 4 interactors.
DIPiDIP-29788N.
IntActiQ91YD9. 10 interactors.
STRINGi10090.ENSMUSP00000031695.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3M3NX-ray7.00W397-474[»]
ProteinModelPortaliQ91YD9.
SMRiQ91YD9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91YD9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 138WH1PROSITE-ProRule annotationAdd BLAST108
Domaini200 – 213CRIBPROSITE-ProRule annotationAdd BLAST14
Domaini401 – 418WH2 1PROSITE-ProRule annotationAdd BLAST18
Domaini429 – 446WH2 2PROSITE-ProRule annotationAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi274 – 385Pro-richAdd BLAST112
Compositional biasi482 – 501Asp-richAdd BLAST20

Sequence similaritiesi

Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 WH1 domain.PROSITE-ProRule annotation
Contains 2 WH2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3671. Eukaryota.
ENOG4111H4S. LUCA.
GeneTreeiENSGT00730000110895.
HOVERGENiHBG000222.
InParanoidiQ91YD9.
KOiK05747.
OMAiWSKKCCG.
OrthoDBiEOG091G0VCD.
PhylomeDBiQ91YD9.
TreeFamiTF316736.

Family and domain databases

CDDicd01205. EVH1_WASP-like. 1 hit.
Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR030214. N-WASP.
IPR011993. PH_dom-like.
IPR011026. WASP_C.
IPR033927. WASPfam_EVH1.
IPR000697. WH1/EVH1_dom.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23202:SF33. PTHR23202:SF33. 1 hit.
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91YD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGQQPPRR VTNVGSLLLT PQENESLFSF LGKKCVTMSS AVVQLYAADR
60 70 80 90 100
NCMWAKKCSG VACLVKDNPQ RSYFLRIFDI KDGKLLWEQE LYNNFVYNSP
110 120 130 140 150
RGYFHTFAGD TCQVALNFAN EEEAKKFRKA VTDLLGRRQR KSEKRRDAPN
160 170 180 190 200
GPNLPMATVD IKNPEITTNR FYGSQVNNIS HTKEKKKGKA KKKRLTKADI
210 220 230 240 250
GTPSNFQHIG HVGWDPNTGF DLNNLDPELK NLFDMCGISE AQLKDRETSK
260 270 280 290 300
VIYDFIEKTG GVEAVKNELR RQAPPPPPPS RGGPPPPPPP PHSSGPPPPP
310 320 330 340 350
ARGRGAPPPP PSRAPTAAPP PPPPSRPGVV VPPPPPNRMY PPPPPALPSS
360 370 380 390 400
APSGPPPPPP PSMAGSTAPP PPPPPPPPPG PPPPPGLPSD GDHQVPAPSG
410 420 430 440 450
NKAALLDQIR EGAQLKKVEQ NSRPVSCSGR DALLDQIRQG IQLKSVSDGQ
460 470 480 490 500
ESTPPTPAPT SGIVGALMEV MQKRSKAIHS SDEDEDDDDE EDFEDDDEWE

D
Length:501
Mass (Da):54,274
Last modified:December 1, 2001 - v1
Checksum:iF5ABF44DF9A9F716
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ318416 mRNA. Translation: CAC69994.1.
CCDSiCCDS19945.1.
RefSeqiNP_082735.2. NM_028459.2.
UniGeneiMm.1574.

Genome annotation databases

EnsembliENSMUST00000031695; ENSMUSP00000031695; ENSMUSG00000029684.
GeneIDi73178.
KEGGimmu:73178.
UCSCiuc009bbv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ318416 mRNA. Translation: CAC69994.1.
CCDSiCCDS19945.1.
RefSeqiNP_082735.2. NM_028459.2.
UniGeneiMm.1574.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3M3NX-ray7.00W397-474[»]
ProteinModelPortaliQ91YD9.
SMRiQ91YD9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215819. 4 interactors.
DIPiDIP-29788N.
IntActiQ91YD9. 10 interactors.
STRINGi10090.ENSMUSP00000031695.

PTM databases

iPTMnetiQ91YD9.
PhosphoSitePlusiQ91YD9.

Proteomic databases

MaxQBiQ91YD9.
PaxDbiQ91YD9.
PRIDEiQ91YD9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031695; ENSMUSP00000031695; ENSMUSG00000029684.
GeneIDi73178.
KEGGimmu:73178.
UCSCiuc009bbv.2. mouse.

Organism-specific databases

CTDi8976.
MGIiMGI:1920428. Wasl.

Phylogenomic databases

eggNOGiKOG3671. Eukaryota.
ENOG4111H4S. LUCA.
GeneTreeiENSGT00730000110895.
HOVERGENiHBG000222.
InParanoidiQ91YD9.
KOiK05747.
OMAiWSKKCCG.
OrthoDBiEOG091G0VCD.
PhylomeDBiQ91YD9.
TreeFamiTF316736.

Enzyme and pathway databases

ReactomeiR-MMU-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-MMU-203641. NOSTRIN mediated eNOS trafficking.
R-MMU-373753. Nephrin interactions.
R-MMU-3928662. EPHB-mediated forward signaling.
R-MMU-418885. DCC mediated attractive signaling.
R-MMU-5663213. RHO GTPases Activate WASPs and WAVEs.
R-MMU-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

ChiTaRSiWasl. mouse.
EvolutionaryTraceiQ91YD9.
PROiQ91YD9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029684.
CleanExiMM_WASL.
ExpressionAtlasiQ91YD9. baseline and differential.
GenevisibleiQ91YD9. MM.

Family and domain databases

CDDicd01205. EVH1_WASP-like. 1 hit.
Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR030214. N-WASP.
IPR011993. PH_dom-like.
IPR011026. WASP_C.
IPR033927. WASPfam_EVH1.
IPR000697. WH1/EVH1_dom.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR23202:SF33. PTHR23202:SF33. 1 hit.
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWASL_MOUSE
AccessioniPrimary (citable) accession number: Q91YD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: December 1, 2001
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.