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Q91YD9

- WASL_MOUSE

UniProt

Q91YD9 - WASL_MOUSE

Protein

Neural Wiskott-Aldrich syndrome protein

Gene

Wasl

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization By similarity. Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.By similarity1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. actin cytoskeleton organization Source: MGI
    2. actin nucleation Source: MGI
    3. cellular protein complex localization Source: MGI
    4. membrane budding Source: BHF-UCL
    5. mitotic nuclear division Source: UniProtKB-KW
    6. positive regulation of clathrin-mediated endocytosis Source: BHF-UCL
    7. positive regulation of filopodium assembly Source: BHF-UCL
    8. regulation of protein localization Source: MGI
    9. regulation of transcription, DNA-templated Source: UniProtKB-KW
    10. response to bacterium Source: MGI
    11. spindle localization Source: MGI
    12. transcription, DNA-templated Source: UniProtKB-KW
    13. vesicle organization Source: BHF-UCL
    14. vesicle transport along actin filament Source: BHF-UCL

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

    Keywords - Ligandi

    Actin-binding

    Enzyme and pathway databases

    ReactomeiREACT_198374. Regulation of actin dynamics for phagocytic cup formation.
    REACT_215995. NOSTRIN mediated eNOS trafficking.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neural Wiskott-Aldrich syndrome protein
    Short name:
    N-WASP
    Gene namesi
    Name:Wasl
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1920428. Wasl.

    Subcellular locationi

    Cytoplasmcytoskeleton. Nucleus
    Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated.

    GO - Cellular componenti

    1. actin cap Source: MGI
    2. cytoplasm Source: MGI
    3. cytoplasmic membrane-bounded vesicle Source: BHF-UCL
    4. cytosol Source: Reactome
    5. lamellipodium Source: MGI
    6. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi253 – 2531Y → E: No effect on phosphorylation. Protein preferentially localized in cytoplasm. 1 Publication
    Mutagenesisi253 – 2531Y → F: Abolishes phosphorylation. Protein preferentially localized in nucleus. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 501500Neural Wiskott-Aldrich syndrome proteinPRO_0000189001Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei239 – 2391Phosphoserine; by TNK2By similarity
    Modified residuei253 – 2531Phosphotyrosine; by FAK1 and TNK24 Publications
    Modified residuei480 – 4801PhosphoserineBy similarity
    Modified residuei481 – 4811PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ91YD9.
    PaxDbiQ91YD9.
    PRIDEiQ91YD9.

    PTM databases

    PhosphoSiteiQ91YD9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ91YD9.
    BgeeiQ91YD9.
    CleanExiMM_WASL.
    GenevestigatoriQ91YD9.

    Interactioni

    Subunit structurei

    Interacts with NOSTRIN, SNX9 and SNX33 By similarity. Binds to TNK2 By similarity. Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11. Interacts with PTK2/FAK1. Interacts with PACSIN1, PACSIN2 and PACSIN3.By similarity4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CttnQ605984EBI-642417,EBI-397955
    Itgb1P090552EBI-642417,EBI-644224
    PdgfrbP056222EBI-642417,EBI-1554855

    Protein-protein interaction databases

    BioGridi215819. 2 interactions.
    DIPiDIP-29788N.
    IntActiQ91YD9. 10 interactions.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3M3NX-ray7.00W397-474[»]
    ProteinModelPortaliQ91YD9.
    SMRiQ91YD9. Positions 26-147, 204-267, 397-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ91YD9.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 138108WH1PROSITE-ProRule annotationAdd
    BLAST
    Domaini200 – 21314CRIBPROSITE-ProRule annotationAdd
    BLAST
    Domaini401 – 41818WH2 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini429 – 44618WH2 2PROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi274 – 385112Pro-richAdd
    BLAST
    Compositional biasi482 – 50120Asp-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CRIB domain.PROSITE-ProRule annotation
    Contains 1 WH1 domain.PROSITE-ProRule annotation
    Contains 2 WH2 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG270974.
    GeneTreeiENSGT00730000110895.
    HOVERGENiHBG000222.
    InParanoidiQ91YD9.
    KOiK05747.
    OMAiPTPAGNK.
    OrthoDBiEOG7FJH20.
    PhylomeDBiQ91YD9.
    TreeFamiTF316736.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    3.90.810.10. 1 hit.
    InterProiIPR000095. CRIB_dom.
    IPR011993. PH_like_dom.
    IPR011026. WASP_C.
    IPR000697. WH1/EVH1.
    IPR003124. WH2_dom.
    [Graphical view]
    PfamiPF00786. PBD. 1 hit.
    PF00568. WH1. 1 hit.
    PF02205. WH2. 2 hits.
    [Graphical view]
    SMARTiSM00285. PBD. 1 hit.
    SM00461. WH1. 1 hit.
    SM00246. WH2. 2 hits.
    [Graphical view]
    SUPFAMiSSF47912. SSF47912. 2 hits.
    PROSITEiPS50108. CRIB. 1 hit.
    PS50229. WH1. 1 hit.
    PS51082. WH2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q91YD9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSGQQPPRR VTNVGSLLLT PQENESLFSF LGKKCVTMSS AVVQLYAADR    50
    NCMWAKKCSG VACLVKDNPQ RSYFLRIFDI KDGKLLWEQE LYNNFVYNSP 100
    RGYFHTFAGD TCQVALNFAN EEEAKKFRKA VTDLLGRRQR KSEKRRDAPN 150
    GPNLPMATVD IKNPEITTNR FYGSQVNNIS HTKEKKKGKA KKKRLTKADI 200
    GTPSNFQHIG HVGWDPNTGF DLNNLDPELK NLFDMCGISE AQLKDRETSK 250
    VIYDFIEKTG GVEAVKNELR RQAPPPPPPS RGGPPPPPPP PHSSGPPPPP 300
    ARGRGAPPPP PSRAPTAAPP PPPPSRPGVV VPPPPPNRMY PPPPPALPSS 350
    APSGPPPPPP PSMAGSTAPP PPPPPPPPPG PPPPPGLPSD GDHQVPAPSG 400
    NKAALLDQIR EGAQLKKVEQ NSRPVSCSGR DALLDQIRQG IQLKSVSDGQ 450
    ESTPPTPAPT SGIVGALMEV MQKRSKAIHS SDEDEDDDDE EDFEDDDEWE 500
    D 501
    Length:501
    Mass (Da):54,274
    Last modified:December 1, 2001 - v1
    Checksum:iF5ABF44DF9A9F716
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ318416 mRNA. Translation: CAC69994.1.
    CCDSiCCDS19945.1.
    RefSeqiNP_082735.2. NM_028459.2.
    UniGeneiMm.1574.

    Genome annotation databases

    EnsembliENSMUST00000031695; ENSMUSP00000031695; ENSMUSG00000029684.
    GeneIDi73178.
    KEGGimmu:73178.
    UCSCiuc009bbv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ318416 mRNA. Translation: CAC69994.1 .
    CCDSi CCDS19945.1.
    RefSeqi NP_082735.2. NM_028459.2.
    UniGenei Mm.1574.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3M3N X-ray 7.00 W 397-474 [» ]
    ProteinModelPortali Q91YD9.
    SMRi Q91YD9. Positions 26-147, 204-267, 397-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 215819. 2 interactions.
    DIPi DIP-29788N.
    IntActi Q91YD9. 10 interactions.

    PTM databases

    PhosphoSitei Q91YD9.

    Proteomic databases

    MaxQBi Q91YD9.
    PaxDbi Q91YD9.
    PRIDEi Q91YD9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031695 ; ENSMUSP00000031695 ; ENSMUSG00000029684 .
    GeneIDi 73178.
    KEGGi mmu:73178.
    UCSCi uc009bbv.2. mouse.

    Organism-specific databases

    CTDi 8976.
    MGIi MGI:1920428. Wasl.

    Phylogenomic databases

    eggNOGi NOG270974.
    GeneTreei ENSGT00730000110895.
    HOVERGENi HBG000222.
    InParanoidi Q91YD9.
    KOi K05747.
    OMAi PTPAGNK.
    OrthoDBi EOG7FJH20.
    PhylomeDBi Q91YD9.
    TreeFami TF316736.

    Enzyme and pathway databases

    Reactomei REACT_198374. Regulation of actin dynamics for phagocytic cup formation.
    REACT_215995. NOSTRIN mediated eNOS trafficking.

    Miscellaneous databases

    ChiTaRSi WASL. mouse.
    EvolutionaryTracei Q91YD9.
    NextBioi 337617.
    PROi Q91YD9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q91YD9.
    Bgeei Q91YD9.
    CleanExi MM_WASL.
    Genevestigatori Q91YD9.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    3.90.810.10. 1 hit.
    InterProi IPR000095. CRIB_dom.
    IPR011993. PH_like_dom.
    IPR011026. WASP_C.
    IPR000697. WH1/EVH1.
    IPR003124. WH2_dom.
    [Graphical view ]
    Pfami PF00786. PBD. 1 hit.
    PF00568. WH1. 1 hit.
    PF02205. WH2. 2 hits.
    [Graphical view ]
    SMARTi SM00285. PBD. 1 hit.
    SM00461. WH1. 1 hit.
    SM00246. WH2. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47912. SSF47912. 2 hits.
    PROSITEi PS50108. CRIB. 1 hit.
    PS50229. WH1. 1 hit.
    PS51082. WH2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility of Shigella flexneri are abolished in N-WASP-defective cells."
      Lommel S., Benesch S., Rottner K., Franz T., Wehland J., Kuehn R.
      EMBO Rep. 2:850-857(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    2. "All three PACSIN isoforms bind to endocytic proteins and inhibit endocytosis."
      Modregger J., Ritter B., Witter B., Paulsson M., Plomann M.
      J. Cell Sci. 113:4511-4521(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PACSIN1; PACSIN2 AND PACSIN3.
    3. "Translocation of N-WASP by nuclear localization and export signals into the nucleus modulates expression of HSP90."
      Suetsugu S., Takenawa T.
      J. Biol. Chem. 278:42515-42523(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-253.
    4. "Tuba, a novel protein containing bin/amphiphysin/Rvs and Dbl homology domains, links dynamin to regulation of the actin cytoskeleton."
      Salazar M.A., Kwiatkowski A.V., Pellegrini L., Cestra G., Butler M.H., Rossman K.L., Serna D.M., Sondek J., Gertler F.B., De Camilli P.
      J. Biol. Chem. 278:49031-49043(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH DNMBP.
    5. "FBP11 regulates nuclear localization of N-WASP and inhibits N-WASP-dependent microspike formation."
      Mizutani K., Suetsugu S., Takenawa T.
      Biochem. Biophys. Res. Commun. 313:468-474(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PRPF40A.
    6. "Focal adhesion kinase regulation of N-WASP subcellular localization and function."
      Wu X., Suetsugu S., Cooper L.A., Takenawa T., Guan J.L.
      J. Biol. Chem. 279:9565-9576(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PTK2/FAK1, SUBCELLULAR LOCATION, PHOSPHORYLATION AT TYR-253.
    7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-253, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    8. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-253, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-253, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiWASL_MOUSE
    AccessioniPrimary (citable) accession number: Q91YD9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2005
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3