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Protein

Neural Wiskott-Aldrich syndrome protein

Gene

Wasl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in mitosis and cytokinesis, via its role in the regulation of actin polymerization (By similarity). Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.By similarity1 Publication

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • actin nucleation Source: MGI
  • cell division Source: UniProtKB-KW
  • cellular protein complex localization Source: MGI
  • membrane budding Source: BHF-UCL
  • mitotic nuclear division Source: UniProtKB-KW
  • negative regulation of membrane tubulation Source: Ensembl
  • positive regulation of Arp2/3 complex-mediated actin nucleation Source: InterPro
  • positive regulation of clathrin-mediated endocytosis Source: BHF-UCL
  • positive regulation of filopodium assembly Source: BHF-UCL
  • regulation of protein localization Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to bacterium Source: MGI
  • spindle localization Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • vesicle organization Source: BHF-UCL
  • vesicle transport along actin filament Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiREACT_293748. Nephrin interactions.
REACT_295531. DCC mediated attractive signaling.
REACT_313804. EPHB-mediated forward signaling.
REACT_344463. Regulation of actin dynamics for phagocytic cup formation.
REACT_351979. NOSTRIN mediated eNOS trafficking.
REACT_358608. RHO GTPases Activate WASPs and WAVEs.

Names & Taxonomyi

Protein namesi
Recommended name:
Neural Wiskott-Aldrich syndrome protein
Short name:
N-WASP
Gene namesi
Name:Wasl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1920428. Wasl.

Subcellular locationi

  • Cytoplasmcytoskeleton
  • Nucleus

  • Note: Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated.

GO - Cellular componenti

  • actin cap Source: MGI
  • cytoplasm Source: MGI
  • cytoplasmic membrane-bounded vesicle Source: BHF-UCL
  • cytosol Source: Reactome
  • extracellular exosome Source: Ensembl
  • lamellipodium Source: MGI
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi253 – 2531Y → E: No effect on phosphorylation. Protein preferentially localized in cytoplasm. 1 Publication
Mutagenesisi253 – 2531Y → F: Abolishes phosphorylation. Protein preferentially localized in nucleus. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 501500Neural Wiskott-Aldrich syndrome proteinPRO_0000189001Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei239 – 2391Phosphoserine; by TNK2By similarity
Modified residuei253 – 2531Phosphotyrosine; by FAK1 and TNK24 Publications
Modified residuei480 – 4801PhosphoserineBy similarity
Modified residuei481 – 4811PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ91YD9.
PaxDbiQ91YD9.
PRIDEiQ91YD9.

PTM databases

PhosphoSiteiQ91YD9.

Expressioni

Gene expression databases

BgeeiQ91YD9.
CleanExiMM_WASL.
ExpressionAtlasiQ91YD9. baseline and differential.
GenevisibleiQ91YD9. MM.

Interactioni

Subunit structurei

Interacts with NOSTRIN, SNX9 and SNX33 (By similarity). Binds to TNK2 (By similarity). Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11. Interacts with PTK2/FAK1. Interacts with PACSIN1, PACSIN2 and PACSIN3.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CttnQ605984EBI-642417,EBI-397955
Itgb1P090552EBI-642417,EBI-644224
PdgfrbP056222EBI-642417,EBI-1554855

Protein-protein interaction databases

BioGridi215819. 2 interactions.
DIPiDIP-29788N.
IntActiQ91YD9. 10 interactions.
STRINGi10090.ENSMUSP00000031695.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3M3NX-ray7.00W397-474[»]
ProteinModelPortaliQ91YD9.
SMRiQ91YD9. Positions 26-147, 204-267, 397-446.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91YD9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 138108WH1PROSITE-ProRule annotationAdd
BLAST
Domaini200 – 21314CRIBPROSITE-ProRule annotationAdd
BLAST
Domaini401 – 41818WH2 1PROSITE-ProRule annotationAdd
BLAST
Domaini429 – 44618WH2 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi274 – 385112Pro-richAdd
BLAST
Compositional biasi482 – 50120Asp-richAdd
BLAST

Sequence similaritiesi

Contains 1 CRIB domain.PROSITE-ProRule annotation
Contains 1 WH1 domain.PROSITE-ProRule annotation
Contains 2 WH2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG270974.
GeneTreeiENSGT00730000110895.
HOVERGENiHBG000222.
InParanoidiQ91YD9.
KOiK05747.
OMAiDAPNGPN.
OrthoDBiEOG7FJH20.
PhylomeDBiQ91YD9.
TreeFamiTF316736.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR030214. N-WASP.
IPR011993. PH_like_dom.
IPR011026. WASP_C.
IPR000697. WH1/EVH1_dom.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR12779:SF3. PTHR12779:SF3. 1 hit.
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91YD9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSGQQPPRR VTNVGSLLLT PQENESLFSF LGKKCVTMSS AVVQLYAADR
60 70 80 90 100
NCMWAKKCSG VACLVKDNPQ RSYFLRIFDI KDGKLLWEQE LYNNFVYNSP
110 120 130 140 150
RGYFHTFAGD TCQVALNFAN EEEAKKFRKA VTDLLGRRQR KSEKRRDAPN
160 170 180 190 200
GPNLPMATVD IKNPEITTNR FYGSQVNNIS HTKEKKKGKA KKKRLTKADI
210 220 230 240 250
GTPSNFQHIG HVGWDPNTGF DLNNLDPELK NLFDMCGISE AQLKDRETSK
260 270 280 290 300
VIYDFIEKTG GVEAVKNELR RQAPPPPPPS RGGPPPPPPP PHSSGPPPPP
310 320 330 340 350
ARGRGAPPPP PSRAPTAAPP PPPPSRPGVV VPPPPPNRMY PPPPPALPSS
360 370 380 390 400
APSGPPPPPP PSMAGSTAPP PPPPPPPPPG PPPPPGLPSD GDHQVPAPSG
410 420 430 440 450
NKAALLDQIR EGAQLKKVEQ NSRPVSCSGR DALLDQIRQG IQLKSVSDGQ
460 470 480 490 500
ESTPPTPAPT SGIVGALMEV MQKRSKAIHS SDEDEDDDDE EDFEDDDEWE

D
Length:501
Mass (Da):54,274
Last modified:December 1, 2001 - v1
Checksum:iF5ABF44DF9A9F716
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ318416 mRNA. Translation: CAC69994.1.
CCDSiCCDS19945.1.
RefSeqiNP_082735.2. NM_028459.2.
UniGeneiMm.1574.

Genome annotation databases

EnsembliENSMUST00000031695; ENSMUSP00000031695; ENSMUSG00000029684.
GeneIDi73178.
KEGGimmu:73178.
UCSCiuc009bbv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ318416 mRNA. Translation: CAC69994.1.
CCDSiCCDS19945.1.
RefSeqiNP_082735.2. NM_028459.2.
UniGeneiMm.1574.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3M3NX-ray7.00W397-474[»]
ProteinModelPortaliQ91YD9.
SMRiQ91YD9. Positions 26-147, 204-267, 397-446.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215819. 2 interactions.
DIPiDIP-29788N.
IntActiQ91YD9. 10 interactions.
STRINGi10090.ENSMUSP00000031695.

PTM databases

PhosphoSiteiQ91YD9.

Proteomic databases

MaxQBiQ91YD9.
PaxDbiQ91YD9.
PRIDEiQ91YD9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031695; ENSMUSP00000031695; ENSMUSG00000029684.
GeneIDi73178.
KEGGimmu:73178.
UCSCiuc009bbv.2. mouse.

Organism-specific databases

CTDi8976.
MGIiMGI:1920428. Wasl.

Phylogenomic databases

eggNOGiNOG270974.
GeneTreeiENSGT00730000110895.
HOVERGENiHBG000222.
InParanoidiQ91YD9.
KOiK05747.
OMAiDAPNGPN.
OrthoDBiEOG7FJH20.
PhylomeDBiQ91YD9.
TreeFamiTF316736.

Enzyme and pathway databases

ReactomeiREACT_293748. Nephrin interactions.
REACT_295531. DCC mediated attractive signaling.
REACT_313804. EPHB-mediated forward signaling.
REACT_344463. Regulation of actin dynamics for phagocytic cup formation.
REACT_351979. NOSTRIN mediated eNOS trafficking.
REACT_358608. RHO GTPases Activate WASPs and WAVEs.

Miscellaneous databases

ChiTaRSiWasl. mouse.
EvolutionaryTraceiQ91YD9.
NextBioi337617.
PROiQ91YD9.
SOURCEiSearch...

Gene expression databases

BgeeiQ91YD9.
CleanExiMM_WASL.
ExpressionAtlasiQ91YD9. baseline and differential.
GenevisibleiQ91YD9. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.90.810.10. 1 hit.
InterProiIPR000095. CRIB_dom.
IPR030214. N-WASP.
IPR011993. PH_like_dom.
IPR011026. WASP_C.
IPR000697. WH1/EVH1_dom.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR12779:SF3. PTHR12779:SF3. 1 hit.
PfamiPF00786. PBD. 1 hit.
PF00568. WH1. 1 hit.
PF02205. WH2. 2 hits.
[Graphical view]
SMARTiSM00285. PBD. 1 hit.
SM00461. WH1. 1 hit.
SM00246. WH2. 2 hits.
[Graphical view]
SUPFAMiSSF47912. SSF47912. 2 hits.
PROSITEiPS50108. CRIB. 1 hit.
PS50229. WH1. 1 hit.
PS51082. WH2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility of Shigella flexneri are abolished in N-WASP-defective cells."
    Lommel S., Benesch S., Rottner K., Franz T., Wehland J., Kuehn R.
    EMBO Rep. 2:850-857(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  2. "All three PACSIN isoforms bind to endocytic proteins and inhibit endocytosis."
    Modregger J., Ritter B., Witter B., Paulsson M., Plomann M.
    J. Cell Sci. 113:4511-4521(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PACSIN1; PACSIN2 AND PACSIN3.
  3. "Translocation of N-WASP by nuclear localization and export signals into the nucleus modulates expression of HSP90."
    Suetsugu S., Takenawa T.
    J. Biol. Chem. 278:42515-42523(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF TYR-253.
  4. "Tuba, a novel protein containing bin/amphiphysin/Rvs and Dbl homology domains, links dynamin to regulation of the actin cytoskeleton."
    Salazar M.A., Kwiatkowski A.V., Pellegrini L., Cestra G., Butler M.H., Rossman K.L., Serna D.M., Sondek J., Gertler F.B., De Camilli P.
    J. Biol. Chem. 278:49031-49043(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DNMBP.
  5. "FBP11 regulates nuclear localization of N-WASP and inhibits N-WASP-dependent microspike formation."
    Mizutani K., Suetsugu S., Takenawa T.
    Biochem. Biophys. Res. Commun. 313:468-474(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PRPF40A.
  6. "Focal adhesion kinase regulation of N-WASP subcellular localization and function."
    Wu X., Suetsugu S., Cooper L.A., Takenawa T., Guan J.L.
    J. Biol. Chem. 279:9565-9576(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PTK2/FAK1, SUBCELLULAR LOCATION, PHOSPHORYLATION AT TYR-253.
  7. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-253, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  8. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-253, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-253, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiWASL_MOUSE
AccessioniPrimary (citable) accession number: Q91YD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: December 1, 2001
Last modified: June 24, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.