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Q91YD3 (DCP1A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
mRNA-decapping enzyme 1A

EC=3.-.-.-
Alternative name(s):
MAD homolog 4-interacting transcription coactivator 1
Smad4-interacting transcriptional co-activator
Transcription factor SMIF
Gene names
Name:Dcp1a
Synonyms:Mitc1, Smif
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1 By similarity.

Subunit structure

Forms a complex with EDC3, DCP2, DDX6 and EDC4/HEDLS, within this complex directly interacts with EDC3. Binds DCP1B, UPF1 and SMAD4. Part of a cytoplasmic complex containing proteins involved in mRNA decay, including XRN1 and LSM1. Interacts with PNRC2 By similarity. Interacts with DDX17 in an RNA-independent manner By similarity. Interacts with ZC3HAV1 By similarity.

Subcellular location

CytoplasmP-body. Nucleus By similarity. Note: Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4 By similarity. Colocalizes with NANOS3 in the processing bodies. Ref.5

Sequence similarities

Belongs to the DCP1 family.

Caution

It is uncertain whether Met-1 or Met-21 is the initiator.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 602602mRNA-decapping enzyme 1A
PRO_0000189633

Amino acid modifications

Modified residue1621Phosphoserine By similarity
Modified residue2001Phosphoserine By similarity
Modified residue3351Phosphoserine By similarity
Modified residue3391Phosphoserine By similarity
Modified residue3721Phosphoserine By similarity
Modified residue4201Phosphothreonine By similarity
Modified residue4411Phosphoserine By similarity
Modified residue5421Phosphoserine By similarity
Modified residue5431Phosphoserine Ref.3
Modified residue5451Phosphoserine Ref.3
Modified residue5511Phosphothreonine By similarity

Natural variations

Natural variant4811Q → R in strain: C57BL/6J. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q91YD3 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 55BC9C32BCA86CFD

FASTA60265,219
        10         20         30         40         50         60 
MALSCSTVRP RRRGSALRSK MELLSRAEQE MSLAALKQHD PYITSIADLT GQVALYTFCP 

        70         80         90        100        110        120 
KANQWEKTDI EGTLFVYRRS ASPYHGFTIV NRLNMHNLVE PVNKDLEFQL HEPFLLYRNA 

       130        140        150        160        170        180 
SLSIYSIWFY DKNDCHRIAK LMADVVEEET RRSQQAARDK QSPSQANGCS DQRPIDILEM 

       190        200        210        220        230        240 
LSRAKDEYER NQMGGSNISS PGLQPSTQLS NLGSTETLEE TPSGSQDKSA PSGHKHLTVE 

       250        260        270        280        290        300 
ELFGTSLPKE QPTAMGLESE DTDKLLGDAS QKEPSSFLPF PFEQSGGAPQ SENLGIHSAA 

       310        320        330        340        350        360 
HHTVQPEVST PVLITPASIA QSGDKHPPSY TLPLSPVLSP TLPAEAPTTQ VPHLPRNSTM 

       370        380        390        400        410        420 
IQAVKTTPRQ KSPLLNQPVP ELSHSSLVAS QSPFRAPVSL ANPAGTALPS VDLLQKLRLT 

       430        440        450        460        470        480 
PQHDQIQAQP LGKGTMAPSF SSAAGQLATP ESFIEPSSKT AAARAAVSAS LSNMVLAPTL 

       490        500        510        520        530        540 
QSMQQNQDPE VFSQPKVLPS AIPIAGSPLV PATTTAVSSV LLSPSVFQQT VPRAADLERK 

       550        560        570        580        590        600 
ASSPSPLTVG TAESQRKPSI ILSKSQLQDT LIHLIKNDSS FLSTLHAVYL QVLTKNKDNH 


NL 

« Hide

References

« Hide 'large scale' references
[1]"SMIF, a Smad4-interacting protein that functions as a co-activator in TGFbeta signalling."
Bai R.-Y., Koester C., Ouyang T., Hahn S.A., Hammerschmidt M., Peschel C., Duyster J.
Nat. Cell Biol. 4:181-190(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Swiss Webster.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-481.
Strain: C57BL/6J.
Tissue: Egg.
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-543 AND SER-545, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[4]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[5]"Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3."
Yamaji M., Tanaka T., Shigeta M., Chuma S., Saga Y., Saitou M.
Reproduction 139:381-393(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ344447 mRNA. Translation: CAC69875.1.
BC066173 mRNA. Translation: AAH66173.1.
RefSeqNP_598522.3. NM_133761.3.
UniGeneMm.28733.

3D structure databases

ProteinModelPortalQ91YD3.
SMRQ91YD3. Positions 21-149, 559-602.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid217832. 1 interaction.
IntActQ91YD3. 4 interactions.

PTM databases

PhosphoSiteQ91YD3.

Proteomic databases

PaxDbQ91YD3.
PRIDEQ91YD3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022535; ENSMUSP00000022535; ENSMUSG00000021962.
GeneID75901.
KEGGmmu:75901.
UCSCuc007sva.1. mouse.

Organism-specific databases

CTD55802.
MGIMGI:1923151. Dcp1a.

Phylogenomic databases

eggNOGNOG314415.
GeneTreeENSGT00390000014855.
HOGENOMHOG000090228.
HOVERGENHBG051320.
InParanoidQ91YD3.
KOK12610.
OMALTPQHDQ.
OrthoDBEOG75QR3K.
PhylomeDBQ91YD3.
TreeFamTF320504.

Gene expression databases

ArrayExpressQ91YD3.
BgeeQ91YD3.
CleanExMM_DCP1A.
GenevestigatorQ91YD3.

Family and domain databases

Gene3D2.30.29.30. 1 hit.
InterProIPR010334. DCP1.
IPR011993. PH_like_dom.
[Graphical view]
PANTHERPTHR16290. PTHR16290. 1 hit.
PfamPF06058. DCP1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio344221.
PROQ91YD3.
SOURCESearch...

Entry information

Entry nameDCP1A_MOUSE
AccessionPrimary (citable) accession number: Q91YD3
Secondary accession number(s): Q6NZE3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: December 1, 2001
Last modified: April 16, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot