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Protein

mRNA-decapping enzyme 1A

Gene

Dcp1a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1 (By similarity).By similarity

GO - Molecular functioni

  1. enzyme regulator activity Source: GO_Central
  2. hydrolase activity Source: UniProtKB-KW
  3. identical protein binding Source: MGI
  4. mRNA binding Source: GO_Central
  5. RNA 7-methylguanosine cap binding Source: GO_Central
  6. transcription factor binding Source: MGI

GO - Biological processi

  1. deadenylation-dependent decapping of nuclear-transcribed mRNA Source: GO_Central
  2. deadenylation-independent decapping of nuclear-transcribed mRNA Source: GO_Central
  3. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB-KW
  4. positive regulation of transcription, DNA-templated Source: MGI
  5. regulation of catalytic activity Source: GOC
  6. transforming growth factor beta receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nonsense-mediated mRNA decay

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA-decapping enzyme 1A (EC:3.-.-.-)
Alternative name(s):
MAD homolog 4-interacting transcription coactivator 1
Smad4-interacting transcriptional co-activator
Transcription factor SMIF
Gene namesi
Name:Dcp1a
Synonyms:Mitc1, Smif
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1923151. Dcp1a.

Subcellular locationi

  1. CytoplasmP-body 1 Publication
  2. Nucleus By similarity

  3. Note: Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4 (By similarity). Colocalizes with NANOS3 in the processing bodies.By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytoplasmic mRNA processing body Source: UniProtKB
  3. membrane Source: MGI
  4. nucleus Source: UniProtKB-SubCell
  5. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602mRNA-decapping enzyme 1APRO_0000189633Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei162 – 1621PhosphoserineBy similarity
Modified residuei200 – 2001PhosphoserineBy similarity
Modified residuei335 – 3351PhosphoserineBy similarity
Modified residuei339 – 3391PhosphoserineBy similarity
Modified residuei372 – 3721PhosphoserineBy similarity
Modified residuei420 – 4201PhosphothreonineBy similarity
Modified residuei441 – 4411PhosphoserineBy similarity
Modified residuei542 – 5421PhosphoserineBy similarity
Modified residuei543 – 5431Phosphoserine1 Publication
Modified residuei545 – 5451Phosphoserine1 Publication
Modified residuei551 – 5511PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ91YD3.
PaxDbiQ91YD3.
PRIDEiQ91YD3.

PTM databases

PhosphoSiteiQ91YD3.

Expressioni

Gene expression databases

BgeeiQ91YD3.
CleanExiMM_DCP1A.
ExpressionAtlasiQ91YD3. baseline and differential.
GenevestigatoriQ91YD3.

Interactioni

Subunit structurei

Forms a complex with EDC3, DCP2, DDX6 and EDC4/HEDLS, within this complex directly interacts with EDC3. Binds DCP1B, UPF1 and SMAD4. Part of a cytoplasmic complex containing proteins involved in mRNA decay, including XRN1 and LSM1. Interacts with PNRC2 (By similarity). Interacts with DDX17 in an RNA-independent manner (By similarity). Interacts with ZC3HAV1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi217832. 1 interaction.
IntActiQ91YD3. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ91YD3.
SMRiQ91YD3. Positions 21-149, 559-602.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DCP1 family.Curated

Phylogenomic databases

eggNOGiNOG314415.
GeneTreeiENSGT00390000014855.
HOGENOMiHOG000090228.
HOVERGENiHBG051320.
InParanoidiQ91YD3.
KOiK12610.
OMAiKHAPSYT.
OrthoDBiEOG75QR3K.
PhylomeDBiQ91YD3.
TreeFamiTF320504.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR010334. DCP1.
IPR011993. PH_like_dom.
[Graphical view]
PANTHERiPTHR16290. PTHR16290. 1 hit.
PfamiPF06058. DCP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91YD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSCSTVRP RRRGSALRSK MELLSRAEQE MSLAALKQHD PYITSIADLT
60 70 80 90 100
GQVALYTFCP KANQWEKTDI EGTLFVYRRS ASPYHGFTIV NRLNMHNLVE
110 120 130 140 150
PVNKDLEFQL HEPFLLYRNA SLSIYSIWFY DKNDCHRIAK LMADVVEEET
160 170 180 190 200
RRSQQAARDK QSPSQANGCS DQRPIDILEM LSRAKDEYER NQMGGSNISS
210 220 230 240 250
PGLQPSTQLS NLGSTETLEE TPSGSQDKSA PSGHKHLTVE ELFGTSLPKE
260 270 280 290 300
QPTAMGLESE DTDKLLGDAS QKEPSSFLPF PFEQSGGAPQ SENLGIHSAA
310 320 330 340 350
HHTVQPEVST PVLITPASIA QSGDKHPPSY TLPLSPVLSP TLPAEAPTTQ
360 370 380 390 400
VPHLPRNSTM IQAVKTTPRQ KSPLLNQPVP ELSHSSLVAS QSPFRAPVSL
410 420 430 440 450
ANPAGTALPS VDLLQKLRLT PQHDQIQAQP LGKGTMAPSF SSAAGQLATP
460 470 480 490 500
ESFIEPSSKT AAARAAVSAS LSNMVLAPTL QSMQQNQDPE VFSQPKVLPS
510 520 530 540 550
AIPIAGSPLV PATTTAVSSV LLSPSVFQQT VPRAADLERK ASSPSPLTVG
560 570 580 590 600
TAESQRKPSI ILSKSQLQDT LIHLIKNDSS FLSTLHAVYL QVLTKNKDNH

NL
Length:602
Mass (Da):65,219
Last modified:December 1, 2001 - v1
Checksum:i55BC9C32BCA86CFD
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti481 – 4811Q → R in strain: C57BL/6J. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ344447 mRNA. Translation: CAC69875.1.
BC066173 mRNA. Translation: AAH66173.1.
CCDSiCCDS36848.1.
RefSeqiNP_598522.3. NM_133761.3.
UniGeneiMm.28733.

Genome annotation databases

EnsembliENSMUST00000022535; ENSMUSP00000022535; ENSMUSG00000021962.
GeneIDi75901.
KEGGimmu:75901.
UCSCiuc007sva.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ344447 mRNA. Translation: CAC69875.1.
BC066173 mRNA. Translation: AAH66173.1.
CCDSiCCDS36848.1.
RefSeqiNP_598522.3. NM_133761.3.
UniGeneiMm.28733.

3D structure databases

ProteinModelPortaliQ91YD3.
SMRiQ91YD3. Positions 21-149, 559-602.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217832. 1 interaction.
IntActiQ91YD3. 4 interactions.

PTM databases

PhosphoSiteiQ91YD3.

Proteomic databases

MaxQBiQ91YD3.
PaxDbiQ91YD3.
PRIDEiQ91YD3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022535; ENSMUSP00000022535; ENSMUSG00000021962.
GeneIDi75901.
KEGGimmu:75901.
UCSCiuc007sva.1. mouse.

Organism-specific databases

CTDi55802.
MGIiMGI:1923151. Dcp1a.

Phylogenomic databases

eggNOGiNOG314415.
GeneTreeiENSGT00390000014855.
HOGENOMiHOG000090228.
HOVERGENiHBG051320.
InParanoidiQ91YD3.
KOiK12610.
OMAiKHAPSYT.
OrthoDBiEOG75QR3K.
PhylomeDBiQ91YD3.
TreeFamiTF320504.

Miscellaneous databases

NextBioi344221.
PROiQ91YD3.
SOURCEiSearch...

Gene expression databases

BgeeiQ91YD3.
CleanExiMM_DCP1A.
ExpressionAtlasiQ91YD3. baseline and differential.
GenevestigatoriQ91YD3.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR010334. DCP1.
IPR011993. PH_like_dom.
[Graphical view]
PANTHERiPTHR16290. PTHR16290. 1 hit.
PfamiPF06058. DCP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "SMIF, a Smad4-interacting protein that functions as a co-activator in TGFbeta signalling."
    Bai R.-Y., Koester C., Ouyang T., Hahn S.A., Hammerschmidt M., Peschel C., Duyster J.
    Nat. Cell Biol. 4:181-190(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss Webster.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-481.
    Strain: C57BL/6J.
    Tissue: Egg.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-543 AND SER-545, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  5. "Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3."
    Yamaji M., Tanaka T., Shigeta M., Chuma S., Saga Y., Saitou M.
    Reproduction 139:381-393(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDCP1A_MOUSE
AccessioniPrimary (citable) accession number: Q91YD3
Secondary accession number(s): Q6NZE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: December 1, 2001
Last modified: March 4, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-21 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.