mRNA-decapping enzyme 1A - Mus musculus (Mouse)

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Q91YD3 (DCP1A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 80. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
mRNA-decapping enzyme 1A
EC=3.-.-.-

Alternative name(s):
MAD homolog 4-interacting transcription coactivator 1
Smad4-interacting transcriptional co-activator
Transcription factor SMIF

Gene names

Name:	Dcp1a
Synonyms:	Mitc1, Smif

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1 By similarity.
Subunit structure	Forms a complex with EDC3, DCP2, DDX6 and EDC4/HEDLS, within this complex directly interacts with EDC3. Binds DCP1B, UPF1 and SMAD4. Part of a cytoplasmic complex containing proteins involved in mRNA decay, including XRN1 and LSM1. Interacts with PNRC2 By similarity. Interacts with DDX17 in an RNA-independent manner By similarity. Interacts with ZC3HAV1 By similarity.
Subcellular location	Cytoplasm › P-body. Nucleus By similarity. Note: Predominantly cytoplasmic, in processing bodies (PB). Nuclear, after TGFB1 treatment. Translocation to the nucleus depends on interaction with SMAD4 By similarity. Co-localizes with NANOS3 in the processing bodies. Ref.5
Sequence similarities	Belongs to the DCP1 family.
Caution	It is uncertain whether Met-1 or Met-21 is the initiator.

Ontologies

Keywords
Biological process	Nonsense-mediated mRNA decay
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Polymorphism
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of transcription, DNA-dependent Inferred from sequence alignment Ref.1. Source: MGI transforming growth factor beta receptor signaling pathway Inferred from sequence alignment Ref.1. Source: MGI
Cellular_component	cytoplasmic mRNA processing body Inferred from direct assay Ref.5. Source: UniProtKB transcription factor complex Inferred from sequence alignment Ref.1. Source: MGI
Molecular_function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW transcription factor binding Inferred from sequence alignment Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 602

602

mRNA-decapping enzyme 1A

PRO_0000189633

Amino acid modifications

Modified residue

162

Phosphoserine By similarity

Modified residue

200

Phosphoserine By similarity

Modified residue

335

Phosphoserine By similarity

Modified residue

339

Phosphoserine By similarity

Modified residue

372

Phosphoserine Ref.4

Modified residue

420

Phosphothreonine By similarity

Modified residue

441

Phosphoserine By similarity

Modified residue

542

Phosphoserine By similarity

Modified residue

543

Phosphoserine Ref.3

Modified residue

545

Phosphoserine Ref.3

Modified residue

551

Phosphothreonine By similarity

Natural variations

Natural variant

481

Q → R in strain: C57BL/6J. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q91YD3 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 55BC9C32BCA86CFD
Show »

FASTA

602

65,219

        10         20         30         40         50         60 
MALSCSTVRP RRRGSALRSK MELLSRAEQE MSLAALKQHD PYITSIADLT GQVALYTFCP 

        70         80         90        100        110        120 
KANQWEKTDI EGTLFVYRRS ASPYHGFTIV NRLNMHNLVE PVNKDLEFQL HEPFLLYRNA 

       130        140        150        160        170        180 
SLSIYSIWFY DKNDCHRIAK LMADVVEEET RRSQQAARDK QSPSQANGCS DQRPIDILEM 

       190        200        210        220        230        240 
LSRAKDEYER NQMGGSNISS PGLQPSTQLS NLGSTETLEE TPSGSQDKSA PSGHKHLTVE 

       250        260        270        280        290        300 
ELFGTSLPKE QPTAMGLESE DTDKLLGDAS QKEPSSFLPF PFEQSGGAPQ SENLGIHSAA 

       310        320        330        340        350        360 
HHTVQPEVST PVLITPASIA QSGDKHPPSY TLPLSPVLSP TLPAEAPTTQ VPHLPRNSTM 

       370        380        390        400        410        420 
IQAVKTTPRQ KSPLLNQPVP ELSHSSLVAS QSPFRAPVSL ANPAGTALPS VDLLQKLRLT 

       430        440        450        460        470        480 
PQHDQIQAQP LGKGTMAPSF SSAAGQLATP ESFIEPSSKT AAARAAVSAS LSNMVLAPTL 

       490        500        510        520        530        540 
QSMQQNQDPE VFSQPKVLPS AIPIAGSPLV PATTTAVSSV LLSPSVFQQT VPRAADLERK 

       550        560        570        580        590        600 
ASSPSPLTVG TAESQRKPSI ILSKSQLQDT LIHLIKNDSS FLSTLHAVYL QVLTKNKDNH 


NL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SMIF, a Smad4-interacting protein that functions as a co-activator in TGFbeta signalling." Bai R.-Y., Koester C., Ouyang T., Hahn S.A., Hammerschmidt M., Peschel C., Duyster J. Nat. Cell Biol. 4:181-190(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss Webster.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-481. Strain: C57BL/6J. Tissue: Egg.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-543 AND SER-545, MASS SPECTROMETRY. Tissue: Liver.
[4]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-372, MASS SPECTROMETRY. Tissue: Melanoma.
[5]	"Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3." Yamaji M., Tanaka T., Shigeta M., Chuma S., Saga Y., Saitou M. Reproduction 139:381-393(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ344447 mRNA. Translation: CAC69875.1. BC066173 mRNA. Translation: AAH66173.1.
IPI	IPI00130114.
RefSeq	NP_598522.3. NM_133761.3.
UniGene	Mm.28733.
3D structure databases
ProteinModelPortal	Q91YD3.
SMR	Q91YD3. Positions 31-149, 559-602.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q91YD3. 1 interaction.
PTM databases
PhosphoSite	Q91YD3.
Proteomic databases
PaxDb	Q91YD3.
PRIDE	Q91YD3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022535; ENSMUSP00000022535; ENSMUSG00000021962.
GeneID	75901.
KEGG	mmu:75901.
UCSC	uc007sva.1. mouse.
Organism-specific databases
CTD	55802.
MGI	MGI:1923151. Dcp1a.
Phylogenomic databases
eggNOG	NOG314415.
GeneTree	ENSGT00390000014855.
HOGENOM	HOG000090228.
HOVERGEN	HBG051320.
InParanoid	Q91YD3.
KO	K12610.
OMA	PSGHKHL.
OrthoDB	EOG4GMTZ4.
Gene expression databases
ArrayExpress	Q91YD3.
Bgee	Q91YD3.
CleanEx	MM_DCP1A.
Genevestigator	Q91YD3.
GermOnline	ENSMUSG00000021962. Mus musculus.
Family and domain databases
Gene3D	2.30.29.30. 1 hit.
InterPro	IPR010334. DCP1. IPR011993. PH_like_dom. [Graphical view]
PANTHER	PTHR16290. PTHR16290. 1 hit.
Pfam	PF06058. DCP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	344221.
SOURCE	Search...

Entry information

Entry name

DCP1A_MOUSE

Accession

Primary (citable) accession number: Q91YD3
Secondary accession number(s): Q6NZE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	December 1, 2001
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents