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Q91Y78

- UCHL3_RAT

UniProt

Q91Y78 - UCHL3_RAT

Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L3

Gene

Uchl3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3, and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Enzyme regulationi

    Inhibited by monoubiquitin and diubiquitin.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei95 – 951NucleophilePROSITE-ProRule annotation
    Active sitei169 – 1691Proton donorPROSITE-ProRule annotation
    Sitei184 – 1841Important for enzyme activityBy similarity

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC12.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase isozyme L3 (EC:3.4.19.12)
    Short name:
    UCH-L3
    Alternative name(s):
    Ubiquitin thioesterase L3
    Gene namesi
    Name:Uchl3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621131. Uchl3.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 230230Ubiquitin carboxyl-terminal hydrolase isozyme L3PRO_0000211063Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei130 – 1301PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ91Y78.
    PRIDEiQ91Y78.

    PTM databases

    PhosphoSiteiQ91Y78.

    Expressioni

    Gene expression databases

    GenevestigatoriQ91Y78.

    Interactioni

    Subunit structurei

    Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000012842.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91Y78.
    SMRiQ91Y78. Positions 2-230.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni8 – 136Interaction with ubiquitinBy similarity
    Regioni152 – 1598Interaction with ubiquitinBy similarity
    Regioni219 – 2246Interaction with ubiquitinBy similarity

    Sequence similaritiesi

    Belongs to the peptidase C12 family.Curated

    Phylogenomic databases

    eggNOGiNOG327708.
    HOGENOMiHOG000182400.
    HOVERGENiHBG075483.
    InParanoidiQ91Y78.
    KOiK05609.
    PhylomeDBiQ91Y78.

    Family and domain databases

    Gene3Di3.40.532.10. 1 hit.
    InterProiIPR001578. Peptidase_C12_UCH.
    [Graphical view]
    PANTHERiPTHR10589. PTHR10589. 1 hit.
    PfamiPF01088. Peptidase_C12. 1 hit.
    [Graphical view]
    PRINTSiPR00707. UBCTHYDRLASE.
    PROSITEiPS00140. UCH_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q91Y78-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMEPE LLSMVPRPVC    50
    AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL 100
    IHAIANNKDK MHFESGSALK KFLEESVAMS PEERARHLEN YDAIRVTHET 150
    SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGKTSDET 200
    LLEDAIEVCK KFMERDPDEL RFNAIALSAA 230
    Length:230
    Mass (Da):26,124
    Last modified:December 1, 2001 - v1
    Checksum:iC793BEFEEB926F10
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB043959 mRNA. Translation: BAB47136.1.
    RefSeqiNP_001103635.1. NM_001110165.1.
    UniGeneiRn.145487.

    Genome annotation databases

    GeneIDi498560.
    KEGGirno:498560.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB043959 mRNA. Translation: BAB47136.1 .
    RefSeqi NP_001103635.1. NM_001110165.1.
    UniGenei Rn.145487.

    3D structure databases

    ProteinModelPortali Q91Y78.
    SMRi Q91Y78. Positions 2-230.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000012842.

    Protein family/group databases

    MEROPSi C12.003.

    PTM databases

    PhosphoSitei Q91Y78.

    Proteomic databases

    PaxDbi Q91Y78.
    PRIDEi Q91Y78.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 498560.
    KEGGi rno:498560.

    Organism-specific databases

    CTDi 7347.
    RGDi 621131. Uchl3.

    Phylogenomic databases

    eggNOGi NOG327708.
    HOGENOMi HOG000182400.
    HOVERGENi HBG075483.
    InParanoidi Q91Y78.
    KOi K05609.
    PhylomeDBi Q91Y78.

    Miscellaneous databases

    NextBioi 700167.

    Gene expression databases

    Genevestigatori Q91Y78.

    Family and domain databases

    Gene3Di 3.40.532.10. 1 hit.
    InterProi IPR001578. Peptidase_C12_UCH.
    [Graphical view ]
    PANTHERi PTHR10589. PTHR10589. 1 hit.
    Pfami PF01088. Peptidase_C12. 1 hit.
    [Graphical view ]
    PRINTSi PR00707. UBCTHYDRLASE.
    PROSITEi PS00140. UCH_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of rat UCHL3."
      Osawa Y.
      Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Brain and Liver.
    2. Lubec G., Chen W.-Q.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 166-186, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiUCHL3_RAT
    AccessioniPrimary (citable) accession number: Q91Y78
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3