Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sialic acid-binding Ig-like lectin 12

Gene

Siglec12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Putative adhesion molecule that mediates sialic-acid dependent binding to cells. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei126Sialic acidBy similarity1

GO - Molecular functioni

  • monosaccharide binding Source: MGI
  • receptor activity Source: MGI
  • sialic acid binding Source: MGI
  • signal transducer activity, downstream of receptor Source: MGI

GO - Biological processi

  • cell adhesion Source: MGI
  • negative regulation of inflammatory response Source: MGI
  • negative regulation of phagocytosis, engulfment Source: MGI

Keywordsi

Biological processCell adhesion
LigandLectin

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialic acid-binding Ig-like lectin 12
Short name:
Siglec-12
Alternative name(s):
Myeloid inhibitory siglec
Short name:
MIS
Sialic acid-binding Ig-like lectin 5
Short name:
Siglec-5
Sialic acid-binding Ig-like lectin E
Short name:
Siglec-E
Short name:
mSiglec-E
Sialic acid-binding Ig-like lectin-like 1
Short name:
Siglec-L1
Gene namesi
Name:Siglec12
Synonyms:Siglec5, Siglece, Siglecl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1932475. Siglece.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 353ExtracellularSequence analysisAdd BLAST335
Transmembranei354 – 374HelicalSequence analysisAdd BLAST21
Topological domaini375 – 467CytoplasmicSequence analysisAdd BLAST93

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi432Y → F: Abolishes binding to PTPN6 and PTPN11. 2 Publications1
Mutagenesisi455Y → F: Reduces binding to PTPN6. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000001495419 – 467Sialic acid-binding Ig-like lectin 12Add BLAST449

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 176PROSITE-ProRule annotation
Disulfide bondi45 ↔ 108PROSITE-ProRule annotation
Glycosylationi46N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi167N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi170 ↔ 219PROSITE-ProRule annotation
Glycosylationi197N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi216N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi227N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi237N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi244N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi262N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi278 ↔ 323PROSITE-ProRule annotation
Glycosylationi287N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi294N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei432Phosphotyrosine1 Publication1
Modified residuei455Phosphotyrosine1 Publication1

Post-translational modificationi

Phosphorylation of Tyr-432 is required for binding to PTPN6 and PTPN11. Phosphorylation of Tyr-455 is involved in binding to PTPN6. Tyr-432 needs to be phosphorylated prior to Tyr-455.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ91Y57.
PaxDbiQ91Y57.
PRIDEiQ91Y57.

PTM databases

iPTMnetiQ91Y57.
PhosphoSitePlusiQ91Y57.

Expressioni

Tissue specificityi

Expressed by monocytic/myeloid lineage cells. Found at higher levels in spleen, liver and heart. Found at lower levels in kidney and lung.

Gene expression databases

BgeeiENSMUSG00000030474.
CleanExiMM_SIGLEC5.
MM_SIGLECE.
ExpressionAtlasiQ91Y57. baseline and differential.
GenevisibleiQ91Y57. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation.2 Publications

Protein-protein interaction databases

BioGridi219910. 5 interactors.
ELMiQ91Y57.
STRINGi10090.ENSMUSP00000032667.

Structurei

3D structure databases

ProteinModelPortaliQ91Y57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 141Ig-like V-typeAdd BLAST121
Domaini152 – 239Ig-like C2-type 1Add BLAST88
Domaini242 – 339Ig-like C2-type 2Add BLAST98

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi430 – 435ITIM motif6
Motifi453 – 458SLAM-like motif6

Domaini

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJT6. Eukaryota.
ENOG410YKZU. LUCA.
GeneTreeiENSGT00760000119139.
HOVERGENiHBG036161.
InParanoidiQ91Y57.
OMAiKTKWNYK.
OrthoDBiEOG091G0445.
TreeFamiTF332441.

Family and domain databases

Gene3Di2.60.40.10. 3 hits.
InterProiView protein in InterPro
IPR007110. Ig-like_dom.
IPR036179. Ig-like_dom_sf.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
PfamiView protein in Pfam
PF07686. V-set. 1 hit.
SMARTiView protein in SMART
SM00409. IG. 3 hits.
SM00408. IGc2. 1 hit.
SUPFAMiSSF48726. SSF48726. 3 hits.
PROSITEiView protein in PROSITE
PS50835. IG_LIKE. 3 hits.
PS00290. IG_MHC. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q91Y57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLLLLLLL WGIKGVEGQN PQEVFTLNVE RKVVVQEGLC VLVPCNFSYL
60 70 80 90 100
KKRLTDWTDS DPVHGFWYRE GTDRRKDSIV ATNNPIRKAV KETRNRFFLL
110 120 130 140 150
GDPWRNDCSL NIREIRKKDA GLYFFRLERG KTKYNYMWDK MTLVVTALTN
160 170 180 190 200
TPQILLPETL EAGHPSNLTC SVPWDCGWTA PPIFSWTGTS VSFLSTNTTG
210 220 230 240 250
SSVLTITPQP QDHGTNLTCQ VTLPGTNVST RMTIRLNVSY APKNLTVTIY
260 270 280 290 300
QGADSVSTIL KNGSSLPISE GQSLRLICST DSYPPANLSW SWDNLTLCPS
310 320 330 340 350
KLSKPGLLEL FPVHLKHGGV YTCQAQHALG SQHISLSLSP QSSATLSEMM
360 370 380 390 400
MGTFVGSGVT ALLFLSVCIL LLAVRSYRRK PARPAVVAPH PDALKVSVSQ
410 420 430 440 450
NPLVESQADD SSEPLPSILE AAPSSTEEEI HYATLSFHEM KPMNLWGQQD
460
TTTEYSEIKF PQRTAWP
Length:467
Mass (Da):51,901
Last modified:July 27, 2011 - v3
Checksum:iE220C29755FE21DE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2L → M in AAK49917 (PubMed:11278955).Curated1
Sequence conflicti24V → G in AAK49917 (PubMed:11278955).Curated1
Sequence conflicti102D → E in AAK49917 (PubMed:11278955).Curated1
Sequence conflicti234I → T in AAG38598 (PubMed:11171044).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF317298 mRNA. Translation: AAG38598.1.
AF329269 mRNA. Translation: AAK49917.1.
AC151989 Genomic DNA. No translation available.
CCDSiCCDS21178.1.
RefSeqiNP_112458.2. NM_031181.2.
UniGeneiMm.157702.

Genome annotation databases

EnsembliENSMUST00000032667; ENSMUSP00000032667; ENSMUSG00000030474.
GeneIDi83382.
KEGGimmu:83382.
UCSCiuc009gng.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiSIG12_MOUSE
AccessioniPrimary (citable) accession number: Q91Y57
Secondary accession number(s): E9QMD1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: July 27, 2011
Last modified: October 25, 2017
This is version 131 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families