Q91XU3 (PI42C_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma
EC=2.7.1.149

Alternative name(s):
Phosphatidylinositol 5-phosphate 4-kinase type II gamma
Short name=PI(5)P 4-kinase type II gamma
Short name=PIP4KII-gamma

Gene names

Name:	Pip4k2c
Synonyms:	Pip5k2c

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May play an important role in the production of Phosphatidylinositol bisphosphate (PIP2), in the endoplasmic reticulum By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.
Subcellular location	Cytoplasm. Membrane. Note: Mostly found in the cytosol and surrounding plasma membrane. However, its presence in the endoplasmic reticulum seems to be a prerequisite for PIP2 synthesis By similarity.
Developmental stage	No significant expression was discerned throughout the prenatal brains except for the olfactory mantle zone, which exhibited the expression signals weakly. On P0 and P7, the expression was weakly positive in the gray matter throughout the brain, with higher expression in the olfactory mitral cell layer. In the cerebellum, the expression was evenly positive in external and internal granule cell layers. On P21 and P56, the expression in non-cortical brain parenchyma was negligible. However, the expression was evident in the olfactory mitral cell layer, the piriform cortex, the hippocampal pyramidal CA1-3, and the cerebellar Purkinje cell layer, although much less distinct in the dentate granule cell layer and the cerebellar granule cell layer. In the cerebral cortex, the expression was weaker in layer V than the other layers. No significant expression was seen in the white matter. Ref.1
Post-translational modification	Phosphorylated By similarity.
Sequence similarities	Contains 1 PIPK domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphatidylinositol phosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: Compara
Molecular_function	1-phosphatidylinositol-5-phosphate 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma		PRO_0000285751
Regions
	Domain	43 – 420	378	PIPK
Amino acid modifications
	Modified residue	26	1	Phosphoserine By similarity
	Modified residue	349	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	90	1	H → R in BAE28464. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q91XU3 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 368C9CE0C9EA83D4	FASTA	421	47,336
	10 20 30 40 50 60 MASSSVPPAT APAAAGGPGP GFGFASKTKK KHFVQQKVKV FRAADPLVGV FLWGVAHSIN 70 80 90 100 110 120 ELSQVPPPVM LLPDDFKASS KIKVNNHFFH RENLPSHFKF KEYCPQVFRN LRDRFAIDDH 130 140 150 160 170 180 DYLVSLTRSP PSETEGSDGR FLISYDRTLV IKEVSSEDIA DMHSNLSNYH QYIVKCHGNT 190 200 210 220 230 240 LLPQFLGMYR VSVENEDSYM LVMRNMFSHR LPVHRKYDLK GSLVSREASD KEKVKELPTL 250 260 270 280 290 300 KDMDFLNKNQ KVYIGEEEKK VFLEKLKRDV EFLVQLKIMD YSLLLGIHDI IRGSEPEEEG 310 320 330 340 350 360 PVREEESEWD GDCNLAGPPA LVGSYGTSPE GIGGYIHSHR PLGPGEFESF IDVYAIRSAE 370 380 390 400 410 420 GAPQKEVYFM GLIDILTQYD AKKKAAHAAK TVKHGAGAEI STVHPEQYAK RFLDFIANIF A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Localization of mRNAs for phosphatidylinositol phosphate kinases in the mouse brain during development." Akiba Y., Suzuki R., Saito-Saino S., Owada Y., Sakagami H., Watanabe M., Kondo H. Gene Expr. Patterns 1:123-133(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. Strain: C57BL/6. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II and FVB/N. Tissue: Kidney and Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB054591 mRNA. Translation: BAB62096.1. AK148299 mRNA. Translation: BAE28464.1. BC005539 mRNA. Translation: AAH05539.1. BC021383 mRNA. Translation: AAH21383.1.
IPI	IPI00129479.
RefSeq	NP_473438.1. NM_054097.3.
UniGene	Mm.22682.
3D structure databases
ProteinModelPortal	Q91XU3.
SMR	Q91XU3. Positions 39-420.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4121758.
STRING	10090.ENSMUSP00000013970.
PTM databases
PhosphoSite	Q91XU3.
Proteomic databases
PaxDb	Q91XU3.
PRIDE	Q91XU3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000013970; ENSMUSP00000013970; ENSMUSG00000025417.
GeneID	117150.
KEGG	mmu:117150.
UCSC	uc007hiq.2. mouse.
Organism-specific databases
CTD	79837.
MGI	MGI:2152214. Pip4k2c.
Phylogenomic databases
eggNOG	COG5253.
GeneTree	ENSGT00690000102069.
HOGENOM	HOG000007832.
HOVERGEN	HBG000072.
InParanoid	Q91XU3.
KO	K00920.
OMA	EGEGDCG.
OrthoDB	EOG4TMR2C.
Gene expression databases
Bgee	Q91XU3.
Genevestigator	Q91XU3.
Family and domain databases
Gene3D	3.30.800.10. 1 hit. 3.30.810.10. 2 hits.
InterPro	IPR023610. PInositol-4-P-5-kinase. IPR027483. PInositol-4-P-5-kinase_C. IPR002498. PInositol-4-P-5-kinase_core. IPR027484. PInositol-4-P-5-kinase_N. IPR016034. PInositol-4P-5-kinase_core_sub. [Graphical view]
PANTHER	PTHR23086. PTHR23086. 1 hit.
Pfam	PF01504. PIP5K. 1 hit. [Graphical view]
SMART	SM00330. PIPKc. 1 hit. [Graphical view]
PROSITE	PS51455. PIPK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	369532.
SOURCE	Search...

Entry information

Entry name

PI42C_MOUSE

Accession

Primary (citable) accession number: Q91XU3
Secondary accession number(s): Q3UFU5, Q99K02

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	December 1, 2001
Last modified:	May 29, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program