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Protein

Protein quaking

Gene

Qki

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation. Required to protect and promote stability of mRNAs such as MBP and CDKN1B which promotes oligodendrocyte differentiation. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of MAG pre-mRNA. Acts as a translational repressor (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei97Involved in RNA bindingBy similarity1
Sitei120Involved in RNA bindingBy similarity1
Sitei124Involved in RNA bindingBy similarity1
Sitei130Involved in RNA bindingBy similarity1
Sitei190Involved in RNA bindingBy similarity1
Sitei193Involved in RNA bindingBy similarity1

GO - Molecular functioni

GO - Biological processi

  • 3'-UTR-mediated mRNA destabilization Source: RGD
  • axon ensheathment Source: RGD
  • brain development Source: RGD
  • long-chain fatty acid biosynthetic process Source: RGD
  • mRNA processing Source: RGD
  • mRNA stabilization Source: RGD
  • mRNA transport Source: UniProtKB-KW
  • muscle cell differentiation Source: RGD
  • myelination Source: RGD
  • negative regulation of cardiac muscle cell apoptotic process Source: RGD
  • positive regulation of gene expression Source: RGD
  • positive regulation of neuron projection development Source: RGD
  • positive regulation of oligodendrocyte differentiation Source: RGD
  • RNA splicing Source: UniProtKB-KW
  • spermatid development Source: RGD
  • vasculogenesis Source: RGD

Keywordsi

Molecular functionDevelopmental protein, RNA-binding
Biological processDifferentiation, mRNA processing, mRNA splicing, mRNA transport, Translation regulation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Protein quaking
Short name:
RqkI
Gene namesi
Name:Qki
Synonyms:Qk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1584886. Qk.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002393761 – 341Protein quakingAdd BLAST341

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei188PhosphoserineBy similarity1
Modified residuei227Omega-N-methylarginineBy similarity1
Modified residuei242Asymmetric dimethylarginine; by CARM1; alternateBy similarity1
Modified residuei242Omega-N-methylarginine; alternateBy similarity1
Modified residuei256Omega-N-methylarginineBy similarity1

Post-translational modificationi

Methylated by PRMT1.By similarity
Tyrosine phosphorylated at its C-terminus, probably by FYN. Phosphorylation leads to decreased mRNA-binding affinity, affecting transport and/or stabilization of MBP mRNA (By similarity).By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ91XU1.
PRIDEiQ91XU1.

PTM databases

iPTMnetiQ91XU1.
PhosphoSitePlusiQ91XU1.

Expressioni

Tissue specificityi

Present in myelinating oligodendrocytes (at protein level).1 Publication

Interactioni

Subunit structurei

Homodimer. Does not require RNA to homodimerize. Able to heterodimerize with BICC1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034413.

Structurei

3D structure databases

ProteinModelPortaliQ91XU1.
SMRiQ91XU1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 153KHAdd BLAST67

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 82Qua1 domain; involved in homodimerizationBy similarityAdd BLAST72
Regioni182 – 213Qua2 domain; involved in RNA bindingBy similarityAdd BLAST32

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi276 – 279SH3-binding4
Motifi324 – 330Nuclear localization signalBy similarity7

Domaini

The KH domain and the Qua2 region are involved in RNA binding.By similarity

Keywords - Domaini

SH3-binding

Phylogenomic databases

eggNOGiKOG1588. Eukaryota.
COG5176. LUCA.
HOGENOMiHOG000019831.
HOVERGENiHBG093883.
InParanoidiQ91XU1.
PhylomeDBiQ91XU1.

Family and domain databases

Gene3Di3.30.1370.10. 1 hit.
InterProiView protein in InterPro
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR036612. KH_dom_type_1_sf.
IPR032367. Quaking_NLS.
IPR032377. STAR_dimer.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF16551. Quaking_NLS. 1 hit.
PF16544. STAR_dimer. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SUPFAMiSSF54791. SSF54791. 1 hit.

Sequencei

Sequence statusi: Complete.

Q91XU1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGEMETKEN PNPTPDYLMQ LMNDKKLMSS LPNFCGIFNH LERLLDEEIS
60 70 80 90 100
RVRKDMYNDT LNGSTEKRSA ELPDAVGPIV QLQEKLYVPV KEYPDFNFVG
110 120 130 140 150
RILGPRGLTA KQLEAETGCK IMVRGKGSMR DKKKEEQNRG KPNWEHLNED
160 170 180 190 200
LHVLITVEDA QNRAEIKLKR AVEEVKKLLV PAAEGEDSLK KMQLMELAIL
210 220 230 240 250
NGTYRDANIK SPALAFSLAA TAQAAPRIIT GPAPVLPPAA LRTPTPAGPT
260 270 280 290 300
IMPLIRQIQT AVMPNGTPHP TAAIVPPGPE AGLIYTPYEY PYTLAPATSI
310 320 330 340
LEYPIEPSGV LGAVATKVRR HDMRVHPYQR IVTADRAATG N
Length:341
Mass (Da):37,643
Last modified:June 13, 2006 - v2
Checksum:i41B066F1711771C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03000138 Genomic DNA. No translation available.
AB054997 mRNA. Translation: BAB62175.1.
UniGeneiRn.228876.

Similar proteinsi

Entry informationi

Entry nameiQKI_RAT
AccessioniPrimary (citable) accession number: Q91XU1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: November 22, 2017
This is version 89 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome