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Protein

ATPase WRNIP1

Gene

Wrnip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a modulator of initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. In the presence of ATP, stimulation of DNA polymerase delta-mediated DNA synthesis is decreased. Plays also a role in the innate immune defense against viruses. Stabilizes the RIG-I/DDX58 dsRNA interaction and promotes RIG-I/DDX58 'Lys-63'-linked polyubiquitination. In turn, RIG-I/DDX58 transmits the signal through mitochondrial MAVS.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi20ZincBy similarity1
Metal bindingi23ZincBy similarity1
Metal bindingi31Zinc; via tele nitrogenBy similarity1
Metal bindingi35Zinc; via tele nitrogenBy similarity1
Metal bindingi39ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri17 – 40UBZ-typeSequence analysisAdd BLAST24
Nucleotide bindingi265 – 271ATPBy similarity7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processDNA damage, DNA replication, Immunity, Innate immunity
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase WRNIP1 (EC:3.6.1.3By similarity)
Alternative name(s):
Werner helicase-interacting protein 1
Gene namesi
Name:Wrnip1Imported
Synonyms:Whip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1926153 Wrnip1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000847861 – 660ATPase WRNIP1Add BLAST660

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei65PhosphoserineBy similarity1
Modified residuei75PhosphoserineCombined sources1
Cross-linki81Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei85PhosphothreonineBy similarity1
Modified residuei91PhosphoserineCombined sources1
Modified residuei92PhosphoserineCombined sources1
Modified residuei116PhosphothreonineBy similarity1
Cross-linki141Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei153PhosphoserineBy similarity1
Cross-linki220Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki296Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki305Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki311Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki317Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki330Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki477Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki477Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei529PhosphotyrosineBy similarity1
Modified residuei557PhosphotyrosineBy similarity1
Cross-linki622Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei628N6-acetyllysine; alternateBy similarity1
Cross-linki628Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki631Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Sumoylated with SUMO1 and SUMO2/3.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ91XU0
PaxDbiQ91XU0
PeptideAtlasiQ91XU0
PRIDEiQ91XU0

PTM databases

iPTMnetiQ91XU0
PhosphoSitePlusiQ91XU0
SwissPalmiQ91XU0

Expressioni

Tissue specificityi

Ubiquitously expressed.By similarity

Gene expression databases

BgeeiENSMUSG00000021400
CleanExiMM_WRNIP1
GenevisibleiQ91XU0 MM

Interactioni

Subunit structurei

Forms homooligomers, possibly octamers. Directly interacts with POLD1, POLD2 and POLD4 (By similarity). Interacts with the N-terminal domain of WRN (By similarity). Interacts (via UBZ-type zinc finger) with monoubiquitin and polyubiquitin. Interacts with TRIM14 and PPP6C; these interactions positively regulate the RIG-I/DDX58 signaling pathway (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi219695, 3 interactors
IntActiQ91XU0, 2 interactors
MINTiQ91XU0
STRINGi10090.ENSMUSP00000021832

Structurei

3D structure databases

ProteinModelPortaliQ91XU0
SMRiQ91XU0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi144 – 210Ala-richPROSITE-ProRule annotationAdd BLAST67

Domaini

The UBZ-type zinc finger binds ubiquitin.By similarity

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri17 – 40UBZ-typeSequence analysisAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2028 Eukaryota
COG2256 LUCA
GeneTreeiENSGT00390000008538
HOVERGENiHBG062192
InParanoidiQ91XU0
KOiK07478
OMAiRIILSQC
OrthoDBiEOG091G09JH
PhylomeDBiQ91XU0
TreeFamiTF324547

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR032423 AAA_assoc_2
IPR003959 ATPase_AAA_core
IPR008921 DNA_pol3_clamp-load_cplx_C
IPR021886 MgsA_C
IPR027417 P-loop_NTPase
IPR006642 Znf_Rad18_put
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF16193 AAA_assoc_2, 1 hit
PF12002 MgsA_C, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00734 ZnF_Rad18, 1 hit
SUPFAMiSSF48019 SSF48019, 1 hit
SSF52540 SSF52540, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q91XU0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVSGPEDDP FLSQLHQVQC PVCQQMMPAA HINSHLDRCL LLHPAGHAEP
60 70 80 90 100
AAGSHRAGER AKGPSPPGAK RRRLSESSAL KQPATPTAAE SSEGEGEEGD
110 120 130 140 150
DGGETESRES YDAPPTPSGA RLIPDFPVAR SSSPARKGMG KRPAAAAAAG
160 170 180 190 200
SASPRSWDEA EAQEEEEAGV DGDGDADVDG EDDPGHWDAD AADASFGVSA
210 220 230 240 250
GRAHPRALAA EEIRQMLEGK PLADKMRPDT LQDYIGQSRA VGQETLLRSL
260 270 280 290 300
LEANEIPSLI LWGPPGCGKT TLAHIIANNS KKHSIRFVTL SATNAKTNDV
310 320 330 340 350
RDVIKQAQNE KSFFKRKTIL FIDEIHRFNK SQQDTFLPHV ECGTITLIGA
360 370 380 390 400
TTENPSFQVN AALLSRCRVI VLEKLPVEAM VTILMRAINS LGIHVLDSSR
410 420 430 440 450
PTDPLSHSSN CSSEPSVFIE DKAVDTLAYL SDGDARTGLN GLQLAVLARL
460 470 480 490 500
SSRKVFCKKS GQTYSPSRVL ITENDVKEGL QRSHILYDRA GEEHYNCISA
510 520 530 540 550
LHKAMRGSDQ NASLYWLARM LEGGEDPLYV ARRLVRFASE DIGLADPSAL
560 570 580 590 600
AQAVAAYQGC HFIGMPECEV LLAQCVVYFA RAPKSIEVYS AYNNVKACLR
610 620 630 640 650
SHQGPLPPVP LHLRNAPTRL MKDLGYGKGY KYNPMYSEPV DQDYLPEELR
660
GVDFFKQRRC
Length:660
Mass (Da):71,794
Last modified:August 16, 2005 - v2
Checksum:iA9114A131B5F9763
GO
Isoform 2Curated (identifier: Q91XU0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     544-568: LADPSALAQAVAAYQGCHFIGMPEC → EWRRVCVGVGVLRGGVLTLVWSHAE
     569-660: Missing.

Note: Due to intron retention. No experimental confirmation available.Curated
Show »
Length:568
Mass (Da):61,406
Checksum:i79994D96D710A287
GO

Sequence cautioni

The sequence AAG35725 differs from that shown. Reason: Frameshift at positions 191, 223 and 233.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180G → V in BAC34213 (PubMed:16141072).Curated1
Sequence conflicti202 – 203RA → P in AAG35725 (Ref. 5) Curated2
Sequence conflicti232Q → H in AAG35725 (Ref. 5) Curated1
Sequence conflicti243Q → E in BAB60708 (PubMed:11301316).Curated1
Sequence conflicti453R → K in BAB60708 (PubMed:11301316).Curated1
Sequence conflicti455V → M in AAH10482 (PubMed:15489334).Curated1
Sequence conflicti499S → C in BAC38404 (PubMed:16141072).Curated1
Sequence conflicti566P → H in BAB60708 (PubMed:11301316).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_051784544 – 568LADPS…GMPEC → EWRRVCVGVGVLRGGVLTLV WSHAE in isoform 2. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_051785569 – 660Missing in isoform 2. 1 PublicationAdd BLAST92

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB056151 mRNA Translation: BAB60708.1
AK041886 mRNA Translation: BAC31091.1
AK050368 mRNA Translation: BAC34213.1
AK082078 mRNA Translation: BAC38404.1
AK167570 mRNA Translation: BAE39633.1
AK170542 mRNA Translation: BAE41868.1
AK170846 mRNA Translation: BAE42069.1
AL645808 Genomic DNA Translation: CAI25647.1
BC010482 mRNA Translation: AAH10482.1
BC058744 mRNA Translation: AAH58744.1
AF208046 mRNA Translation: AAG35725.1 Frameshift.
CCDSiCCDS26427.1 [Q91XU0-1]
RefSeqiNP_084491.3, NM_030215.3 [Q91XU0-1]
UniGeneiMm.286680

Genome annotation databases

EnsembliENSMUST00000021832; ENSMUSP00000021832; ENSMUSG00000021400 [Q91XU0-1]
GeneIDi78903
KEGGimmu:78903
UCSCiuc007pzs.2 mouse [Q91XU0-2]
uc007pzt.2 mouse [Q91XU0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiWRIP1_MOUSE
AccessioniPrimary (citable) accession number: Q91XU0
Secondary accession number(s): Q3TCT7
, Q6PDF0, Q8BUW5, Q8BWP6, Q8BY55, Q921W3, Q9EQL3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: May 23, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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