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Q91XU0 (WRIP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATPase WRNIP1

EC=3.6.1.3
Alternative name(s):
Werner helicase-interacting protein 1
Gene names
Name:Wrnip1
Synonyms:Whip
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length660 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate. UniProtKB Q96S55

Subunit structure

Homooligomer; most likely an octamer. Interacts with POLD1, POLD2 an POLD4. Interacts (via UBZ-type zinc finger) with polyubiquitin By similarity. Interacts with the N-terminal domain of WRN. Ref.1

Subcellular location

Nucleus. Note: Colocalizes with WRN in granular structures in the nucleus. Ref.1

Post-translational modification

Sumoylated with SUMO1 and SUMO2/3 By similarity.

Sequence similarities

Belongs to the AAA ATPase family.

Sequence caution

The sequence AAG35725.1 differs from that shown. Reason: Frameshift at positions 191, 223 and 233.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q91XU0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q91XU0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     544-568: LADPSALAQAVAAYQGCHFIGMPEC → EWRRVCVGVGVLRGGVLTLVWSHAE
     569-660: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 660660ATPase WRNIP1
PRO_0000084786

Regions

Nucleotide binding263 – 2708ATP Potential

Amino acid modifications

Modified residue751Phosphoserine By similarity
Modified residue851Phosphothreonine By similarity
Modified residue911Phosphoserine By similarity
Modified residue921Phosphoserine Ref.6
Modified residue1161Phosphothreonine By similarity
Modified residue1531Phosphoserine By similarity
Modified residue5291Phosphotyrosine By similarity
Modified residue5571Phosphotyrosine By similarity
Modified residue6281N6-acetyllysine By similarity

Natural variations

Alternative sequence544 – 56825LADPS…GMPEC → EWRRVCVGVGVLRGGVLTLV WSHAE in isoform 2.
VSP_051784
Alternative sequence569 – 66092Missing in isoform 2.
VSP_051785

Experimental info

Sequence conflict1801G → V in BAC34213. Ref.2
Sequence conflict202 – 2032RA → P in AAG35725. Ref.5
Sequence conflict2321Q → H in AAG35725. Ref.5
Sequence conflict2431Q → E in BAB60708. Ref.1
Sequence conflict4531R → K in BAB60708. Ref.1
Sequence conflict4551V → M in AAH10482. Ref.4
Sequence conflict4991S → C in BAC38404. Ref.2
Sequence conflict5661P → H in BAB60708. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 16, 2005. Version 2.
Checksum: A9114A131B5F9763

FASTA66071,794
        10         20         30         40         50         60 
MEVSGPEDDP FLSQLHQVQC PVCQQMMPAA HINSHLDRCL LLHPAGHAEP AAGSHRAGER 

        70         80         90        100        110        120 
AKGPSPPGAK RRRLSESSAL KQPATPTAAE SSEGEGEEGD DGGETESRES YDAPPTPSGA 

       130        140        150        160        170        180 
RLIPDFPVAR SSSPARKGMG KRPAAAAAAG SASPRSWDEA EAQEEEEAGV DGDGDADVDG 

       190        200        210        220        230        240 
EDDPGHWDAD AADASFGVSA GRAHPRALAA EEIRQMLEGK PLADKMRPDT LQDYIGQSRA 

       250        260        270        280        290        300 
VGQETLLRSL LEANEIPSLI LWGPPGCGKT TLAHIIANNS KKHSIRFVTL SATNAKTNDV 

       310        320        330        340        350        360 
RDVIKQAQNE KSFFKRKTIL FIDEIHRFNK SQQDTFLPHV ECGTITLIGA TTENPSFQVN 

       370        380        390        400        410        420 
AALLSRCRVI VLEKLPVEAM VTILMRAINS LGIHVLDSSR PTDPLSHSSN CSSEPSVFIE 

       430        440        450        460        470        480 
DKAVDTLAYL SDGDARTGLN GLQLAVLARL SSRKVFCKKS GQTYSPSRVL ITENDVKEGL 

       490        500        510        520        530        540 
QRSHILYDRA GEEHYNCISA LHKAMRGSDQ NASLYWLARM LEGGEDPLYV ARRLVRFASE 

       550        560        570        580        590        600 
DIGLADPSAL AQAVAAYQGC HFIGMPECEV LLAQCVVYFA RAPKSIEVYS AYNNVKACLR 

       610        620        630        640        650        660 
SHQGPLPPVP LHLRNAPTRL MKDLGYGKGY KYNPMYSEPV DQDYLPEELR GVDFFKQRRC 

« Hide

Isoform 2 [UniParc].

Checksum: 79994D96D710A287
Show »

FASTA56861,406

References

« Hide 'large scale' references
[1]"A novel protein interacts with the Werner's syndrome gene product physically and functionally."
Kawabe Y., Branzei D., Hayashi T., Suzuki H., Masuko T., Onoda F., Heo S.-J., Ikeda H., Shimamoto A., Furuichi Y., Seki M., Enomoto T.
J. Biol. Chem. 276:20364-20369(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH WRN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: C57BL/6.
Tissue: Testis.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J and NOD.
Tissue: Cerebellum, Liver, Placenta and Thymus.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: Czech II and FVB/N-3.
Tissue: Mammary gland.
[5]"Characterization of RuvB homologs in human and mouse."
Shannon M., Ramirez M., Thelen M.P.
Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 182-660 (ISOFORM 1).
[6]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB056151 mRNA. Translation: BAB60708.1.
AK041886 mRNA. Translation: BAC31091.1.
AK050368 mRNA. Translation: BAC34213.1.
AK082078 mRNA. Translation: BAC38404.1.
AK167570 mRNA. Translation: BAE39633.1.
AK170542 mRNA. Translation: BAE41868.1.
AK170846 mRNA. Translation: BAE42069.1.
AL645808 Genomic DNA. Translation: CAI25647.1.
BC010482 mRNA. Translation: AAH10482.1.
BC058744 mRNA. Translation: AAH58744.1.
AF208046 mRNA. Translation: AAG35725.1. Frameshift.
CCDSCCDS26427.1. [Q91XU0-1]
RefSeqNP_084491.3. NM_030215.3. [Q91XU0-1]
UniGeneMm.286680.

3D structure databases

ProteinModelPortalQ91XU0.
SMRQ91XU0. Positions 12-46, 220-655.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid219695. 2 interactions.
IntActQ91XU0. 2 interactions.
MINTMINT-4113108.

PTM databases

PhosphoSiteQ91XU0.

Proteomic databases

MaxQBQ91XU0.
PaxDbQ91XU0.
PRIDEQ91XU0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000021832; ENSMUSP00000021832; ENSMUSG00000021400. [Q91XU0-1]
GeneID78903.
KEGGmmu:78903.
UCSCuc007pzs.2. mouse. [Q91XU0-2]
uc007pzt.2. mouse. [Q91XU0-1]

Organism-specific databases

CTD56897.
MGIMGI:1926153. Wrnip1.

Phylogenomic databases

eggNOGCOG2256.
GeneTreeENSGT00390000008538.
HOVERGENHBG062192.
InParanoidQ91XU0.
KOK07478.
OMAPGHWDAD.
OrthoDBEOG744T8W.
PhylomeDBQ91XU0.
TreeFamTF324547.

Gene expression databases

BgeeQ91XU0.
CleanExMM_WRNIP1.
GenevestigatorQ91XU0.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR008921. DNA_pol3_clamp-load_cplx_C.
IPR021886. MgsA_C.
IPR027417. P-loop_NTPase.
IPR006642. Znf_Rad18_put.
[Graphical view]
PfamPF00004. AAA. 1 hit.
PF12002. MgsA_C. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00734. ZnF_Rad18. 1 hit.
[Graphical view]
SUPFAMSSF48019. SSF48019. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

NextBio349714.
PROQ91XU0.
SOURCESearch...

Entry information

Entry nameWRIP1_MOUSE
AccessionPrimary (citable) accession number: Q91XU0
Secondary accession number(s): Q3TCT7 expand/collapse secondary AC list , Q6PDF0, Q8BUW5, Q8BWP6, Q8BY55, Q921W3, Q9EQL3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: July 9, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot