Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q91XU0

- WRIP1_MOUSE

UniProt

Q91XU0 - WRIP1_MOUSE

Protein

ATPase WRNIP1

Gene

Wrnip1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (16 Aug 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Functions as a modulator for initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. Has an intrinsic ATPase activity that functions as a sensor of DNA damage or of arrested replication forks and regulates the extent of DNA synthesis By similarity.By similarity

    Catalytic activityi

    ATP + H2O = ADP + phosphate.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi263 – 2708ATPSequence Analysis

    GO - Molecular functioni

    1. ATPase activity Source: UniProtKB
    2. ATP binding Source: UniProtKB-KW
    3. DNA binding Source: InterPro
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. DNA synthesis involved in DNA repair Source: UniProtKB
    3. regulation of DNA-dependent DNA replication initiation Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    DNA damage, DNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATPase WRNIP1 (EC:3.6.1.3)
    Alternative name(s):
    Werner helicase-interacting protein 1
    Gene namesi
    Name:Wrnip1Imported
    Synonyms:Whip
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1926153. Wrnip1.

    Subcellular locationi

    Nucleus 1 Publication
    Note: Colocalizes with WRN in granular structures in the nucleus.

    GO - Cellular componenti

    1. mitochondrion Source: Ensembl
    2. nucleus Source: UniProtKB
    3. perinuclear region of cytoplasm Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 660660ATPase WRNIP1PRO_0000084786Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei75 – 751PhosphoserineBy similarity
    Modified residuei85 – 851PhosphothreonineBy similarity
    Modified residuei91 – 911PhosphoserineBy similarity
    Modified residuei92 – 921Phosphoserine1 Publication
    Modified residuei116 – 1161PhosphothreonineBy similarity
    Modified residuei153 – 1531PhosphoserineBy similarity
    Modified residuei529 – 5291PhosphotyrosineBy similarity
    Modified residuei557 – 5571PhosphotyrosineBy similarity
    Modified residuei628 – 6281N6-acetyllysineBy similarity

    Post-translational modificationi

    Sumoylated with SUMO1 and SUMO2/3.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    MaxQBiQ91XU0.
    PaxDbiQ91XU0.
    PRIDEiQ91XU0.

    PTM databases

    PhosphoSiteiQ91XU0.

    Expressioni

    Gene expression databases

    BgeeiQ91XU0.
    CleanExiMM_WRNIP1.
    GenevestigatoriQ91XU0.

    Interactioni

    Subunit structurei

    Homooligomer; most likely an octamer. Interacts with POLD1, POLD2 an POLD4. Interacts (via UBZ-type zinc finger) with polyubiquitin By similarity. Interacts with the N-terminal domain of WRN.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi219695. 2 interactions.
    IntActiQ91XU0. 2 interactions.
    MINTiMINT-4113108.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91XU0.
    SMRiQ91XU0. Positions 12-46, 220-655.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the AAA ATPase family.Sequence Analysis

    Phylogenomic databases

    eggNOGiCOG2256.
    GeneTreeiENSGT00390000008538.
    HOVERGENiHBG062192.
    InParanoidiQ91XU0.
    KOiK07478.
    OMAiPGHWDAD.
    OrthoDBiEOG744T8W.
    PhylomeDBiQ91XU0.
    TreeFamiTF324547.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR008921. DNA_pol3_clamp-load_cplx_C.
    IPR021886. MgsA_C.
    IPR027417. P-loop_NTPase.
    IPR006642. Znf_Rad18_put.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    PF12002. MgsA_C. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    SM00734. ZnF_Rad18. 1 hit.
    [Graphical view]
    SUPFAMiSSF48019. SSF48019. 1 hit.
    SSF52540. SSF52540. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 11 Publication (identifier: Q91XU0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEVSGPEDDP FLSQLHQVQC PVCQQMMPAA HINSHLDRCL LLHPAGHAEP    50
    AAGSHRAGER AKGPSPPGAK RRRLSESSAL KQPATPTAAE SSEGEGEEGD 100
    DGGETESRES YDAPPTPSGA RLIPDFPVAR SSSPARKGMG KRPAAAAAAG 150
    SASPRSWDEA EAQEEEEAGV DGDGDADVDG EDDPGHWDAD AADASFGVSA 200
    GRAHPRALAA EEIRQMLEGK PLADKMRPDT LQDYIGQSRA VGQETLLRSL 250
    LEANEIPSLI LWGPPGCGKT TLAHIIANNS KKHSIRFVTL SATNAKTNDV 300
    RDVIKQAQNE KSFFKRKTIL FIDEIHRFNK SQQDTFLPHV ECGTITLIGA 350
    TTENPSFQVN AALLSRCRVI VLEKLPVEAM VTILMRAINS LGIHVLDSSR 400
    PTDPLSHSSN CSSEPSVFIE DKAVDTLAYL SDGDARTGLN GLQLAVLARL 450
    SSRKVFCKKS GQTYSPSRVL ITENDVKEGL QRSHILYDRA GEEHYNCISA 500
    LHKAMRGSDQ NASLYWLARM LEGGEDPLYV ARRLVRFASE DIGLADPSAL 550
    AQAVAAYQGC HFIGMPECEV LLAQCVVYFA RAPKSIEVYS AYNNVKACLR 600
    SHQGPLPPVP LHLRNAPTRL MKDLGYGKGY KYNPMYSEPV DQDYLPEELR 650
    GVDFFKQRRC 660
    Length:660
    Mass (Da):71,794
    Last modified:August 16, 2005 - v2
    Checksum:iA9114A131B5F9763
    GO
    Isoform 2Curated (identifier: Q91XU0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         544-568: LADPSALAQAVAAYQGCHFIGMPEC → EWRRVCVGVGVLRGGVLTLVWSHAE
         569-660: Missing.

    Note: No experimental confirmation available.Curated

    Show »
    Length:568
    Mass (Da):61,406
    Checksum:i79994D96D710A287
    GO

    Sequence cautioni

    The sequence AAG35725.1 differs from that shown. Reason: Frameshift at positions 191, 223 and 233.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti180 – 1801G → V in BAC34213. (PubMed:16141072)Curated
    Sequence conflicti202 – 2032RA → P in AAG35725. 1 PublicationCurated
    Sequence conflicti232 – 2321Q → H in AAG35725. 1 PublicationCurated
    Sequence conflicti243 – 2431Q → E in BAB60708. (PubMed:11301316)Curated
    Sequence conflicti453 – 4531R → K in BAB60708. (PubMed:11301316)Curated
    Sequence conflicti455 – 4551V → M in AAH10482. (PubMed:15489334)Curated
    Sequence conflicti499 – 4991S → C in BAC38404. (PubMed:16141072)Curated
    Sequence conflicti566 – 5661P → H in BAB60708. (PubMed:11301316)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei544 – 56825LADPS…GMPEC → EWRRVCVGVGVLRGGVLTLV WSHAE in isoform 2. 1 PublicationVSP_051784Add
    BLAST
    Alternative sequencei569 – 66092Missing in isoform 2. 1 PublicationVSP_051785Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB056151 mRNA. Translation: BAB60708.1.
    AK041886 mRNA. Translation: BAC31091.1.
    AK050368 mRNA. Translation: BAC34213.1.
    AK082078 mRNA. Translation: BAC38404.1.
    AK167570 mRNA. Translation: BAE39633.1.
    AK170542 mRNA. Translation: BAE41868.1.
    AK170846 mRNA. Translation: BAE42069.1.
    AL645808 Genomic DNA. Translation: CAI25647.1.
    BC010482 mRNA. Translation: AAH10482.1.
    BC058744 mRNA. Translation: AAH58744.1.
    AF208046 mRNA. Translation: AAG35725.1. Frameshift.
    CCDSiCCDS26427.1. [Q91XU0-1]
    RefSeqiNP_084491.3. NM_030215.3. [Q91XU0-1]
    UniGeneiMm.286680.

    Genome annotation databases

    EnsembliENSMUST00000021832; ENSMUSP00000021832; ENSMUSG00000021400. [Q91XU0-1]
    GeneIDi78903.
    KEGGimmu:78903.
    UCSCiuc007pzs.2. mouse. [Q91XU0-2]
    uc007pzt.2. mouse. [Q91XU0-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB056151 mRNA. Translation: BAB60708.1 .
    AK041886 mRNA. Translation: BAC31091.1 .
    AK050368 mRNA. Translation: BAC34213.1 .
    AK082078 mRNA. Translation: BAC38404.1 .
    AK167570 mRNA. Translation: BAE39633.1 .
    AK170542 mRNA. Translation: BAE41868.1 .
    AK170846 mRNA. Translation: BAE42069.1 .
    AL645808 Genomic DNA. Translation: CAI25647.1 .
    BC010482 mRNA. Translation: AAH10482.1 .
    BC058744 mRNA. Translation: AAH58744.1 .
    AF208046 mRNA. Translation: AAG35725.1 . Frameshift.
    CCDSi CCDS26427.1. [Q91XU0-1 ]
    RefSeqi NP_084491.3. NM_030215.3. [Q91XU0-1 ]
    UniGenei Mm.286680.

    3D structure databases

    ProteinModelPortali Q91XU0.
    SMRi Q91XU0. Positions 12-46, 220-655.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 219695. 2 interactions.
    IntActi Q91XU0. 2 interactions.
    MINTi MINT-4113108.

    PTM databases

    PhosphoSitei Q91XU0.

    Proteomic databases

    MaxQBi Q91XU0.
    PaxDbi Q91XU0.
    PRIDEi Q91XU0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021832 ; ENSMUSP00000021832 ; ENSMUSG00000021400 . [Q91XU0-1 ]
    GeneIDi 78903.
    KEGGi mmu:78903.
    UCSCi uc007pzs.2. mouse. [Q91XU0-2 ]
    uc007pzt.2. mouse. [Q91XU0-1 ]

    Organism-specific databases

    CTDi 56897.
    MGIi MGI:1926153. Wrnip1.

    Phylogenomic databases

    eggNOGi COG2256.
    GeneTreei ENSGT00390000008538.
    HOVERGENi HBG062192.
    InParanoidi Q91XU0.
    KOi K07478.
    OMAi PGHWDAD.
    OrthoDBi EOG744T8W.
    PhylomeDBi Q91XU0.
    TreeFami TF324547.

    Miscellaneous databases

    NextBioi 349714.
    PROi Q91XU0.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q91XU0.
    CleanExi MM_WRNIP1.
    Genevestigatori Q91XU0.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR008921. DNA_pol3_clamp-load_cplx_C.
    IPR021886. MgsA_C.
    IPR027417. P-loop_NTPase.
    IPR006642. Znf_Rad18_put.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    PF12002. MgsA_C. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    SM00734. ZnF_Rad18. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48019. SSF48019. 1 hit.
    SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A novel protein interacts with the Werner's syndrome gene product physically and functionally."
      Kawabe Y., Branzei D., Hayashi T., Suzuki H., Masuko T., Onoda F., Heo S.-J., Ikeda H., Shimamoto A., Furuichi Y., Seki M., Enomoto T.
      J. Biol. Chem. 276:20364-20369(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH WRN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: C57BL/6Imported.
      Tissue: TestisImported.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J and NOD.
      Tissue: Cerebellum, Liver, Placenta and Thymus.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: Czech IIImported and FVB/N-3Imported.
      Tissue: Mammary glandImported.
    5. "Characterization of RuvB homologs in human and mouse."
      Shannon M., Ramirez M., Thelen M.P.
      Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 182-660 (ISOFORM 1).
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-92, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiWRIP1_MOUSE
    AccessioniPrimary (citable) accession number: Q91XU0
    Secondary accession number(s): Q3TCT7
    , Q6PDF0, Q8BUW5, Q8BWP6, Q8BY55, Q921W3, Q9EQL3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2005
    Last sequence update: August 16, 2005
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3