ID   BCDO1_RAT               Reviewed;         566 AA.
AC   Q91XT5;
DT   10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   16-JUN-2009, entry version 40.
DE   RecName: Full=Beta,beta-carotene 15,15'-monooxygenase;
DE            EC=1.14.99.36;
DE   AltName: Full=Beta-carotene dioxygenase 1;
GN   Name=Bcmo1; Synonyms=Bcdo, Bcdo1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Intestine;
RA   Takitani K., Ban R., Tamai H.;
RT   "Regulation of beta-carotene 15,15'-dioxygenase in oxidative stress.";
RL   Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Symmetrically cleaves beta-carotene into two molecules
CC       of retinal. The reaction proceeds in three stages, epoxidation of
CC       the 15,15'-double bond, hydration of the double bond leading to
CC       ring opening, and oxidative cleavage of the diol formed (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: Beta-carotene + O(2) = 2 retinal.
CC   -!- COFACTOR: Iron (By similarity).
CC   -!- PATHWAY: Cofactor metabolism; retinol metabolism.
CC   -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
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DR   EMBL; AB062912; BAB60807.1; -; mRNA.
DR   IPI; IPI00208162; -.
DR   RefSeq; NP_446100.1; -.
DR   UniGene; Rn.126587; -.
DR   PRIDE; Q91XT5; -.
DR   Ensembl; ENSRNOG00000012027; Rattus norvegicus.
DR   GeneID; 114106; -.
DR   KEGG; rno:114106; -.
DR   RGD; 70981; Bcmo1.
DR   HOVERGEN; Q91XT5; -.
DR   BRENDA; 1.14.99.36; 248.
DR   NextBio; 618281; -.
DR   ArrayExpress; Q91XT5; -.
DR   GermOnline; ENSRNOG00000012027; Rattus norvegicus.
DR   GO; GO:0003834; F:beta-carotene 15,15'-monooxygenase activity; IDA:RGD.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR004294; Carotenoid_Oase.
DR   PANTHER; PTHR10543; Carotenoid_Oase; 1.
DR   Pfam; PF03055; RPE65; 1.
PE   2: Evidence at transcript level;
KW   Dioxygenase; Iron; Oxidoreductase.
FT   CHAIN         1    566       Beta,beta-carotene 15,15'-monooxygenase.
FT                                /FTId=PRO_0000143935.
SQ   SEQUENCE   566 AA;  63637 MW;  A1FF8A47BA6CE6E5 CRC64;
     MEIIFGRNKK EQLEPLRATV TGSIPAWLQG TLLRNGPGMH TVGDSKYNHW FDGLALLHSF
     SIRDGEVFYR SKYLQSDTYN ANIEANRIVV SEFGTMAYPD PCKNIFSKAF SYLSHTIPDF
     TDNCLINIMK CGEDFYATTE TNYIRKIDPQ TLETLEKVDY RKYVAVNLAT SHPHYDEAGN
     VLNMGTSIAD KGGTKYVMFK IPATAPGSKK KGKNPLKHSE VFCSIPSRSL LSPSYYHSFG
     VTENYVVFLE QPFKLDILKM ATAYMRGVSW ASCMTFCKED KTYIHIIDQK TRKPVPTKFY
     TDPMVVFHHV NAYEEDGCVL FDVIAYEDNS LYQLFYLANL NKDFEEKSRL TSVPTLRRFA
     VPLHVDKDAE VGSNLVKVSS TTATALKEKD DHVYCQPEVL YEGLELPRIN YAHNGKPYRY
     IFAAEVQWSP VPTKILKYDV LTKSSLKWSE ESCWPAEPLF VPTPGAKDED DGVILSAIIS
     TDPQKLPFLL ILDAKSFTEL ARASVDVDMH LDLHGLFIPD AGWNAVKQTP AKTQEDENSD
     HPTGLTAPGL GHGENDFTAG HGGKSL
//