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Protein

Phospholipid hydroperoxide glutathione peroxidase, nuclear

Gene

Gpx4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Stabilizes the condensed chromatin in sperm nuclei and is necessary for male fertility.By similarity1 Publication

Catalytic activityi

2 glutathione + a hydroperoxy-fatty-acyl-[lipid] = glutathione disulfide + a hydroxy-fatty-acyl-[lipid] + H2O.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei129 – 1291By similarity

GO - Molecular functioni

GO - Biological processi

  • cellular oxidant detoxification Source: GOC
  • chromatin organization Source: MGI
  • multicellular organism development Source: UniProtKB-KW
  • response to oxidative stress Source: InterPro
  • spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Oxidoreductase, Peroxidase

Protein family/group databases

PeroxiBasei3865. MmGPx04-B.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid hydroperoxide glutathione peroxidase, nuclear (EC:1.11.1.12)
Alternative name(s):
Glutathione peroxidase 4
Short name:
GPx-4
Short name:
GSHPx-4
Gene namesi
Name:Gpx4
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:104767. Gpx4.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • mitochondrial inner membrane Source: MGI
  • mitochondrion Source: MGI
  • nuclear envelope Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253Phospholipid hydroperoxide glutathione peroxidase, nuclearPRO_0000066625Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei78 – 781PhosphoserineBy similarity
Modified residuei96 – 961PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ91XR9.
MaxQBiQ91XR9.
PaxDbiQ91XR9.
PRIDEiQ91XR9.

PTM databases

iPTMnetiQ91XR9.
PhosphoSiteiQ91XR9.
SwissPalmiQ91XR9.

Expressioni

Tissue specificityi

Expressed exclusively in sperm.1 Publication

Gene expression databases

CleanExiMM_GPX4.

Interactioni

Protein-protein interaction databases

BioGridi551619. 1 interaction.
STRINGi10090.ENSMUSP00000094863.

Structurei

3D structure databases

ProteinModelPortaliQ91XR9.
SMRiQ91XR9. Positions 89-253.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutathione peroxidase family.Curated

Phylogenomic databases

eggNOGiKOG1651. Eukaryota.
COG0386. LUCA.
HOGENOMiHOG000277054.
HOVERGENiHBG004333.
InParanoidiQ91XR9.
KOiK05361.
PhylomeDBiQ91XR9.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000889. Glutathione_peroxidase.
IPR029759. GPX_AS.
IPR029760. GPX_CS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11592. PTHR11592. 1 hit.
PfamiPF00255. GSHPx. 1 hit.
[Graphical view]
PRINTSiPR01011. GLUTPROXDASE.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Nuclear1 Publication (identifier: Q91XR9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRAAARKRG RCRQRGGSPR GRRRRGPGRQ SPRKRPGPRR RKARARRRRR
60 70 80 90 100
ARPRRMEPIP EPFNPGPLLQ EPPQYCNSSE FLGLCASRDD WRCARSMHEF
110 120 130 140 150
SAKDIDGHMV CLDKYRGFVC IVTNVASQUG KTDVNYTQLV DLHARYAECG
160 170 180 190 200
LRILAFPCNQ FGRQEPGSNQ EIKEFAAGYN VKFDMYSKIC VNGDDAHPLW
210 220 230 240 250
KWMKVQPKGR GMLGNAIKWN FTKFLIDKNG CEVKRYGPME EPQVIERDLP

CYL
Length:253
Mass (Da):29,252
Last modified:February 26, 2008 - v3
Checksum:iC55E282753231653
GO
Isoform MitochondrialCurated (identifier: O70325-1) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry O70325.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:197
Mass (Da):22,229
GO
Isoform Cytoplasmic (identifier: O70325-2) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry O70325.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by alternative initiation at Met-28 of isoform Mitochondrial.
Length:170
Mass (Da):19,522
GO

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei129 – 1291SelenocysteineBy similarity

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF274027 mRNA. Translation: AAK74112.1.
AB030643 Genomic DNA. Translation: BAC06509.1.
CCDSiCCDS24007.1. [Q91XR9-1]
RefSeqiNP_001032830.2. NM_001037741.3.
UniGeneiMm.332810.
Mm.359573.

Genome annotation databases

GeneIDi625249.
KEGGimmu:625249.

Keywords - Coding sequence diversityi

Alternative splicing, Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF274027 mRNA. Translation: AAK74112.1.
AB030643 Genomic DNA. Translation: BAC06509.1.
CCDSiCCDS24007.1. [Q91XR9-1]
RefSeqiNP_001032830.2. NM_001037741.3.
UniGeneiMm.332810.
Mm.359573.

3D structure databases

ProteinModelPortaliQ91XR9.
SMRiQ91XR9. Positions 89-253.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi551619. 1 interaction.
STRINGi10090.ENSMUSP00000094863.

Protein family/group databases

PeroxiBasei3865. MmGPx04-B.

PTM databases

iPTMnetiQ91XR9.
PhosphoSiteiQ91XR9.
SwissPalmiQ91XR9.

Proteomic databases

EPDiQ91XR9.
MaxQBiQ91XR9.
PaxDbiQ91XR9.
PRIDEiQ91XR9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi625249.
KEGGimmu:625249.

Organism-specific databases

CTDi2879.
MGIiMGI:104767. Gpx4.

Phylogenomic databases

eggNOGiKOG1651. Eukaryota.
COG0386. LUCA.
HOGENOMiHOG000277054.
HOVERGENiHBG004333.
InParanoidiQ91XR9.
KOiK05361.
PhylomeDBiQ91XR9.

Miscellaneous databases

ChiTaRSiGpx4. mouse.
SOURCEiSearch...

Gene expression databases

CleanExiMM_GPX4.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000889. Glutathione_peroxidase.
IPR029759. GPX_AS.
IPR029760. GPX_CS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11592. PTHR11592. 1 hit.
PfamiPF00255. GSHPx. 1 hit.
[Graphical view]
PRINTSiPR01011. GLUTPROXDASE.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a specific sperm nuclei selenoenzyme necessary for protamine thiol cross-linking during sperm maturation."
    Pfeifer H., Conrad M., Roethlein D., Kyriakopoulos A., Brielmeier M., Bornkamm G.W., Behne D.
    FASEB J. 15:1236-1238(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: TestisImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
    Tissue: Liver.
  3. "The selenoprotein GPX4 is essential for mouse development and protects from radiation and oxidative damage insults."
    Yant L.J., Ran Q., Rao L., Van Remmen H., Shibatani T., Belter J.G., Motta L., Richardson A., Prolla T.A.
    Free Radic. Biol. Med. 34:496-502(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiGPX42_MOUSE
AccessioniPrimary (citable) accession number: Q91XR9
Secondary accession number(s): Q8K4U8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: February 26, 2008
Last modified: June 8, 2016
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.