Phospholipid hydroperoxide glutathione peroxidase, nuclear

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Molecule processing

Sites

Amino acid modifications

Keywords

Gene Ontology (GO)

Molecule processing

Sites

Amino acid modifications

Sequence databases

3D structure databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

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Reviewed, UniProtKB/Swiss-Prot Q91XR9 (GPX42_MOUSE)

Last modified November 25, 2008. Version 53. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Protein names

Recommended name:
    Phospholipid hydroperoxide glutathione peroxidase, nuclear
    EC=1.11.1.12
Alternative name(s):
    GPX-4

Gene names

Name:

Gpx4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

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Function	Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Stabilizes the condensed chromatin in sperm nuclei and is necessary for male fertility.
Catalytic activity	2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H(2)O.
Subcellular location	Nucleus.
Tissue specificity	Expressed exclusively in sperm.
Sequence similarities	Belongs to the glutathione peroxidase family.

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Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Selenocysteine
Ligand	Selenium
Molecular function	Developmental protein Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	establishment or maintenance of chromatin architecture Ref.1 Inferred from direct assay. Source: MGI multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Ref.1 Inferred from direct assay. Source: MGI
Cellular component	cytosol Ref.3 Inferred from direct assay. Source: MGI mitochondrial inner membrane Inferred from direct assay. Source: MGI nuclear envelope Ref.3 Inferred from direct assay. Source: MGI
Molecular function	glutathione peroxidase activity Ref.1 Inferred from direct assay. Source: MGI phospholipid-hydroperoxide glutathione peroxidase activity Inferred from electronic annotation. Source: EC selenium binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 253

253

Phospholipid hydroperoxide glutathione peroxidase, nuclear

PRO_0000066625

Active site

129

By similarity

Non-standard residue

129

Selenocysteine By similarity

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Sequence

Length

Mass (Da)

Tools

Isoform Nuclear [UniParc].

Last modified February 26, 2008. Version 3.
Checksum: C55E282753231653
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FASTA

253

29,252

        10         20         30         40         50         60 
MGRAAARKRG RCRQRGGSPR GRRRRGPGRQ SPRKRPGPRR RKARARRRRR ARPRRMEPIP 

        70         80         90        100        110        120 
EPFNPGPLLQ EPPQYCNSSE FLGLCASRDD WRCARSMHEF SAKDIDGHMV CLDKYRGFVC 

       130        140        150        160        170        180 
IVTNVASQUG KTDVNYTQLV DLHARYAECG LRILAFPCNQ FGRQEPGSNQ EIKEFAAGYN 

       190        200        210        220        230        240 
VKFDMYSKIC VNGDDAHPLW KWMKVQPKGR GMLGNAIKWN FTKFLIDKNG CEVKRYGPME 

       250 
EPQVIERDLP CYL

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Isoform Mitochondrial.
See O70325.

–

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[1]	"Identification of a specific sperm nuclei selenoenzyme necessary for protamine thiol cross-linking during sperm maturation." Pfeifer H., Conrad M., Roethlein D., Kyriakopoulos A., Brielmeier M., Bornkamm G.W., Behne D. FASEB J. 15:1236-1238(2001) [PubMed: 11344099] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Testis.
[2]	"Early embryonic lethality caused by targeted disruption of the mouse PHGPx gene." Imai H., Hirao F., Sakamoto T., Sekine K., Mizukura Y., Saito M., Kitamoto T., Hayasaka M., Hanaoka K., Nakagawa Y. Biochem. Biophys. Res. Commun. 305:278-286(2003) [PubMed: 12745070] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ. Tissue: Liver.
[3]	"The selenoprotein GPX4 is essential for mouse development and protects from radiation and oxidative damage insults." Yant L.J., Ran Q., Rao L., Van Remmen H., Shibatani T., Belter J.G., Motta L., Richardson A., Prolla T.A. Free Radic. Biol. Med. 34:496-502(2003) [PubMed: 12566075] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

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Sequence databases
	AF274027 mRNA. Translation: AAK74112.1. AB030643 Genomic DNA. Translation: BAC06509.1.
RefSeq	NP_001032830.2.
UniGene	Mm.359573
3D structure databases
HSSP	HSSP built from PDB template 1GP1 based on UniProtKB P00435.
SMR	Q91XR9. Positions 92-253.
ModBase	Search...
Protein family/group databases
PeroxiBase	3865. MmGPx04-b.
Genome annotation databases
Ensembl	ENSMUSG00000075706. Mus musculus. [Contig view]
GeneID	625249.
KEGG	mmu:625249.
Organism-specific databases
MGI	MGI:104767. Gpx4.
Phylogenomic databases
HOGENOM	Q91XR9.
HOVERGEN	Q91XR9.
Gene expression databases
CleanEx	MM_GPX4.
GermOnline	ENSMUSG00000075706. Mus musculus.
Family and domain databases
InterPro	IPR000889. Glut_peroxidase. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PRINTS	PR01011. GLUTPROXDASE.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	418158.
SOURCE	Search...

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Entry name

GPX42_MOUSE

Accession

Primary (citable) accession number: Q91XR9
Secondary accession number(s): Q8K4U8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	February 26, 2008
Last modified:	November 25, 2008
This is version 53 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Nuclear (identifier: Q91XR9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Mitochondrial (identifier: O70325-1)

The sequence of this isoform can be found in the external entry O70325-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.