ID   GPX42_RAT               Reviewed;         253 AA.
AC   Q91XR8; Q6PZ55;
DT   23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 3.
DT   16-JUN-2009, entry version 52.
DE   RecName: Full=Phospholipid hydroperoxide glutathione peroxidase, nuclear;
DE            EC=1.11.1.12;
DE   AltName: Full=GPX-4;
GN   Name=Gpx4;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Wistar;
RA   Maiorino M., Scapin M., Ursini F., Biasolo M., Bosello V., Flohe L.;
RT   "Distinct promoters determine alternative transcription of gpx-4 into
RT   PHGPx variants.";
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Wistar;
RA   Tramer F., Vetere A., Martinelli M., Paroni F., Marsich E., Sandri G.,
RA   Panfili E.;
RT   "Cloning of cDNA of rat phospholipid hydroperoxide/sperm nucleus
RT   glutathione peroxidase.";
RL   Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-250, AND SUBCELLULAR LOCATION.
RC   TISSUE=Testis;
RX   MEDLINE=21242617; PubMed=11344099;
RA   Pfeifer H., Conrad M., Roethlein D., Kyriakopoulos A., Brielmeier M.,
RA   Bornkamm G.W., Behne D.;
RT   "Identification of a specific sperm nuclei selenoenzyme necessary for
RT   protamine thiol cross-linking during sperm maturation.";
RL   FASEB J. 15:1236-1238(2001).
CC   -!- FUNCTION: Could play a major role in protecting mammals from the
CC       toxicity of ingested lipid hydroperoxides. Essential for embryonic
CC       development. Protects from radiation and oxidative damage.
CC       Stabilizes the condensed chromatin in sperm nuclei and is
CC       necessary male fertility (By similarity).
CC   -!- CATALYTIC ACTIVITY: 2 glutathione + a lipid hydroperoxide =
CC       glutathione disulfide + lipid + 2 H(2)O.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=Nuclear;
CC         IsoId=Q91XR8-1; Sequence=Displayed;
CC       Name=Mitochondrial;
CC         IsoId=P36970-1; Sequence=External;
CC   -!- SIMILARITY: Belongs to the glutathione peroxidase family.
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DR   EMBL; AJ537598; CAD61278.1; -; Genomic_DNA.
DR   EMBL; AY570513; AAS76675.1; -; mRNA.
DR   EMBL; AF274028; AAK74113.1; -; mRNA.
DR   IPI; IPI00208098; -.
DR   RefSeq; NP_001034938.1; -.
DR   UniGene; Rn.3647; -.
DR   HSSP; P00435; 1GP1.
DR   SMR; Q91XR8; 92-253.
DR   PeroxiBase; 3813; RnoGPx04-c.
DR   GeneID; 29328; -.
DR   KEGG; rno:29328; -.
DR   RGD; 69226; Gpx4.
DR   HOVERGEN; Q91XR8; -.
DR   BRENDA; 1.11.1.12; 248.
DR   NextBio; 608793; -.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0005625; C:soluble fraction; IDA:RGD.
DR   GO; GO:0043295; F:glutathione binding; IDA:RGD.
DR   GO; GO:0004602; F:glutathione peroxidase activity; ISS:UniProtKB.
DR   GO; GO:0047066; F:phospholipid-hydroperoxide glutathione pero...; IDA:RGD.
DR   GO; GO:0008430; F:selenium binding; IDA:RGD.
DR   GO; GO:0007568; P:aging; IEP:RGD.
DR   GO; GO:0006325; P:establishment or maintenance of chromatin a...; ISS:UniProtKB.
DR   GO; GO:0006749; P:glutathione metabolic process; IDA:RGD.
DR   GO; GO:0042744; P:hydrogen peroxide catabolic process; IDA:RGD.
DR   GO; GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0050727; P:regulation of inflammatory response; IMP:RGD.
DR   GO; GO:0032355; P:response to estradiol stimulus; IEP:RGD.
DR   GO; GO:0007283; P:spermatogenesis; ISS:UniProtKB.
DR   InterPro; IPR000889; Glutathione_peroxidase.
DR   InterPro; IPR012335; Thioredoxin_fold.
DR   Gene3D; G3DSA:3.40.30.10; Thioredoxin_fold; 1.
DR   PANTHER; PTHR11592; Glut_peroxidase; 1.
DR   Pfam; PF00255; GSHPx; 1.
DR   PRINTS; PR01011; GLUTPROXDASE.
DR   PROSITE; PS00460; GLUTATHIONE_PEROXID_1; 1.
DR   PROSITE; PS00763; GLUTATHIONE_PEROXID_2; 1.
DR   PROSITE; PS51355; GLUTATHIONE_PEROXID_3; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Developmental protein; Nucleus; Oxidoreductase;
KW   Peroxidase; Selenium; Selenocysteine.
FT   CHAIN         1    253       Phospholipid hydroperoxide glutathione
FT                                peroxidase, nuclear.
FT                                /FTId=PRO_0000066626.
FT   ACT_SITE    129    129
FT   NON_STD     129    129       Selenocysteine.
FT   CONFLICT      9     14       RGRCRQ -> PGRQCAGIRPYGP (in Ref. 3;
FT                                AAK74113).
FT   CONFLICT    101    101       A -> S (in Ref. 1; CAD61278).
FT   CONFLICT    192    192       N -> S (in Ref. 2; AAS76675).
SQ   SEQUENCE   253 AA;  29335 MW;  D108602E22DAD29A CRC64;
     MGRAAARKRG RCRQRGRSPG GRRRREPGRQ SPRKRPGPRR RRARARRRRR ARPRRMEPIP
     EPFNPRPLLQ DLPQTSNSHE FLGLCASRDD WRCARSMHEF AAKDIDGHMV CLDKYRGCVC
     IVTNVASQUG KTDVNYTQLV DLHARYAECG LRILAFPCNQ FGRQEPGSNQ EIKEFAAGYN
     VRFDMYSKIC VNGDDAHPLW KWMKVQPKGR GMLGNAIKWN FTKFLIDKNG CVVKRYGPME
     EPQVIEKDLP CYL
//