Phospholipid hydroperoxide glutathione peroxidase, nuclear

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Alternative products

Sequence annotation (Features)

Sequences

References

Cross-references

Entry information

Relevant documents

Molecule processing

Sites

Amino acid modifications

Experimental info

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

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Reviewed, UniProtKB/Swiss-Prot Q91XR8 (GPX42_RAT)

Last modified February 9, 2010. Version 58. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Protein names

Recommended name:
    Phospholipid hydroperoxide glutathione peroxidase, nuclear
    EC=1.11.1.12
Alternative name(s):
    Glutathione peroxidase 4
      Short name=GSHPx-4
      Short name=GPx-4

Gene names

Name:

Gpx4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

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Function	Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Stabilizes the condensed chromatin in sperm nuclei and is necessary male fertility By similarity. UniProtKB O70325 UniProtKB P36970
Catalytic activity	2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H₂O.
Subcellular location	Nucleus Ref.3.
Sequence similarities	Belongs to the glutathione peroxidase family.

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Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Selenocysteine
Ligand	Selenium
Molecular function	Developmental protein Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	aging Inferred from expression pattern. Source: RGD chromatin organization Inferred from sequence or structural similarity. Source: UniProtKB glutathione metabolic process Inferred from direct assay. Source: RGD hydrogen peroxide catabolic process Inferred from direct assay. Source: RGD multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of inflammatory response Inferred from mutant phenotype. Source: RGD response to estradiol stimulus Inferred from expression pattern. Source: RGD spermatogenesis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB soluble fraction Inferred from direct assay. Source: RGD
Molecular function	glutathione binding Inferred from direct assay. Source: RGD glutathione peroxidase activity Inferred from sequence or structural similarity. Source: UniProtKB phospholipid-hydroperoxide glutathione peroxidase activity Inferred from direct assay. Source: RGD selenium binding Inferred from direct assay. Source: RGD
Complete GO annotation...

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Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Chain

1 – 253

253

Phospholipid hydroperoxide glutathione peroxidase, nuclear

PRO_0000066626

Active site

129

Non-standard residue

129

Selenocysteine

Sequence conflict

9 – 14

RGRCRQ → PGRQCAGIRPYGP in AAK74113. Ref.3

Sequence conflict

101

A → S in CAD61278. Ref.1

Sequence conflict

192

N → S in AAS76675. Ref.2

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Sequence

Length

Mass (Da)

Tools

Isoform Nuclear [UniParc].

Last modified February 26, 2008. Version 3.
Checksum: D108602E22DAD29A
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FASTA

253

29,335

        10         20         30         40         50         60 
MGRAAARKRG RCRQRGRSPG GRRRREPGRQ SPRKRPGPRR RRARARRRRR ARPRRMEPIP 

        70         80         90        100        110        120 
EPFNPRPLLQ DLPQTSNSHE FLGLCASRDD WRCARSMHEF AAKDIDGHMV CLDKYRGCVC 

       130        140        150        160        170        180 
IVTNVASQUG KTDVNYTQLV DLHARYAECG LRILAFPCNQ FGRQEPGSNQ EIKEFAAGYN 

       190        200        210        220        230        240 
VRFDMYSKIC VNGDDAHPLW KWMKVQPKGR GMLGNAIKWN FTKFLIDKNG CVVKRYGPME 

       250 
EPQVIEKDLP CYL

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Isoform Mitochondrial.

See P36970.

FASTA

–

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[1]	"Distinct promoters determine alternative transcription of gpx-4 into PHGPx variants." Maiorino M., Scapin M., Ursini F., Biasolo M., Bosello V., Flohe L. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Wistar.
[2]	"Cloning of cDNA of rat phospholipid hydroperoxide/sperm nucleus glutathione peroxidase." Tramer F., Vetere A., Martinelli M., Paroni F., Marsich E., Sandri G., Panfili E. Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
[3]	"Identification of a specific sperm nuclei selenoenzyme necessary for protamine thiol cross-linking during sperm maturation." Pfeifer H., Conrad M., Roethlein D., Kyriakopoulos A., Brielmeier M., Bornkamm G.W., Behne D. FASEB J. 15:1236-1238(2001) [PubMed: 11344099] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-250, SUBCELLULAR LOCATION. Tissue: Testis.
+	Additional computationally mapped references.

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Sequence databases
EMBL GenBank DDBJ	AJ537598 Genomic DNA. Translation: CAD61278.1. AY570513 mRNA. Translation: AAS76675.1. AF274028 mRNA. Translation: AAK74113.1.
IPI	IPI00208098.
RefSeq	NP_001034938.1.
UniGene	Rn.3647
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q91XR8.
Protein family/group databases
PeroxiBase	3813. RnoGPx04-c.
Genome annotation databases
GeneID	29328.
KEGG	rno:29328.
Organism-specific databases
CTD	29328.
RGD	69226. Gpx4.
Phylogenomic databases
HOVERGEN	Q91XR8.
PhylomeDB	Q91XR8.
Enzyme and pathway databases
BRENDA	1.11.1.12. 248.
Gene expression databases
Genevestigator	Q91XR8.
Family and domain databases
InterPro	IPR000889. Glutathione_peroxidase. IPR012336. Thioredoxin-like_fold. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PRINTS	PR01011. GLUTPROXDASE.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	608793.

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Entry name

GPX42_RAT

Accession

Primary (citable) accession number: Q91XR8
Secondary accession number(s): Q6PZ55

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 23, 2003
Last sequence update:	February 26, 2008
Last modified:	February 9, 2010
This is version 58 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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SIMILARITY comments

Index of protein domains and families

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Nuclear Ref.3 (identifier: Q91XR8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Mitochondrial (identifier: P36970-1)

The sequence of this isoform can be found in the external entry P36970-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.