Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phospholipid hydroperoxide glutathione peroxidase, nuclear

Gene

Gpx4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Stabilizes the condensed chromatin in sperm nuclei and is necessary male fertility.By similarity

Catalytic activityi

2 glutathione + a hydroperoxy-fatty-acyl-[lipid] = glutathione disulfide + a hydroxy-fatty-acyl-[lipid] + H2O.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei129 – 1291

GO - Molecular functioni

  • glutathione binding Source: RGD
  • glutathione peroxidase activity Source: UniProtKB
  • phospholipid-hydroperoxide glutathione peroxidase activity Source: RGD
  • selenium binding Source: RGD

GO - Biological processi

  • aging Source: RGD
  • cellular oxidant detoxification Source: GOC
  • chromatin organization Source: UniProtKB
  • glutathione metabolic process Source: RGD
  • hydrogen peroxide catabolic process Source: RGD
  • multicellular organism development Source: UniProtKB-KW
  • regulation of inflammatory response Source: RGD
  • response to estradiol Source: RGD
  • response to oxidative stress Source: InterPro
  • spermatogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Oxidoreductase, Peroxidase

Protein family/group databases

PeroxiBasei3813. RnoGPx04-C.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid hydroperoxide glutathione peroxidase, nuclear (EC:1.11.1.12)
Alternative name(s):
Glutathione peroxidase 4
Short name:
GPx-4
Short name:
GSHPx-4
Gene namesi
Name:Gpx4
OrganismiRattus norvegicus (Rat)Imported
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69226. Gpx4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253Phospholipid hydroperoxide glutathione peroxidase, nuclearPRO_0000066626Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei78 – 781PhosphoserineCombined sources
Modified residuei96 – 961PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ91XR8.
PRIDEiQ91XR8.

PTM databases

iPTMnetiQ91XR8.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018691.

Structurei

3D structure databases

ProteinModelPortaliQ91XR8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutathione peroxidase family.Curated

Phylogenomic databases

eggNOGiKOG1651. Eukaryota.
COG0386. LUCA.
HOVERGENiHBG004333.
InParanoidiQ91XR8.
KOiK05361.
PhylomeDBiQ91XR8.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000889. Glutathione_peroxidase.
IPR029759. GPX_AS.
IPR029760. GPX_CS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11592. PTHR11592. 1 hit.
PfamiPF00255. GSHPx. 1 hit.
[Graphical view]
PRINTSiPR01011. GLUTPROXDASE.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Nuclear1 Publication (identifier: Q91XR8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRAAARKRG RCRQRGRSPG GRRRREPGRQ SPRKRPGPRR RRARARRRRR
60 70 80 90 100
ARPRRMEPIP EPFNPRPLLQ DLPQTSNSHE FLGLCASRDD WRCARSMHEF
110 120 130 140 150
AAKDIDGHMV CLDKYRGCVC IVTNVASQUG KTDVNYTQLV DLHARYAECG
160 170 180 190 200
LRILAFPCNQ FGRQEPGSNQ EIKEFAAGYN VRFDMYSKIC VNGDDAHPLW
210 220 230 240 250
KWMKVQPKGR GMLGNAIKWN FTKFLIDKNG CVVKRYGPME EPQVIEKDLP

CYL
Length:253
Mass (Da):29,335
Last modified:February 26, 2008 - v3
Checksum:iD108602E22DAD29A
GO
Isoform MitochondrialCurated (identifier: P36970-1) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry P36970.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:197
Mass (Da):22,225
GO
Isoform Cytoplasmic (identifier: P36970-2) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry P36970.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by alternative initiation at Met-28 of isoform Mitochondrial.
Length:170
Mass (Da):19,490
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 146RGRCRQ → PGRQCAGIRPYGP in AAK74113 (PubMed:11344099).Curated
Sequence conflicti101 – 1011A → S in CAD61278 (Ref. 1) Curated
Sequence conflicti192 – 1921N → S in AAS76675 (Ref. 2) Curated

Non-standard residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-standard residuei129 – 1291SelenocysteineCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ537598 Genomic DNA. Translation: CAD61278.1.
AY570513 mRNA. Translation: AAS76675.1.
AF274028 mRNA. Translation: AAK74113.1.
RefSeqiNP_001034938.1. NM_001039849.2. [Q91XR8-1]
UniGeneiRn.3647.

Genome annotation databases

GeneIDi29328.
KEGGirno:29328.
UCSCiRGD:69226. rat. [Q91XR8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Selenocysteine

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ537598 Genomic DNA. Translation: CAD61278.1.
AY570513 mRNA. Translation: AAS76675.1.
AF274028 mRNA. Translation: AAK74113.1.
RefSeqiNP_001034938.1. NM_001039849.2. [Q91XR8-1]
UniGeneiRn.3647.

3D structure databases

ProteinModelPortaliQ91XR8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018691.

Protein family/group databases

PeroxiBasei3813. RnoGPx04-C.

PTM databases

iPTMnetiQ91XR8.

Proteomic databases

PaxDbiQ91XR8.
PRIDEiQ91XR8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29328.
KEGGirno:29328.
UCSCiRGD:69226. rat. [Q91XR8-1]

Organism-specific databases

CTDi2879.
RGDi69226. Gpx4.

Phylogenomic databases

eggNOGiKOG1651. Eukaryota.
COG0386. LUCA.
HOVERGENiHBG004333.
InParanoidiQ91XR8.
KOiK05361.
PhylomeDBiQ91XR8.

Miscellaneous databases

NextBioi608793.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR000889. Glutathione_peroxidase.
IPR029759. GPX_AS.
IPR029760. GPX_CS.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PANTHERiPTHR11592. PTHR11592. 1 hit.
PfamiPF00255. GSHPx. 1 hit.
[Graphical view]
PRINTSiPR01011. GLUTPROXDASE.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Distinct promoters determine alternative transcription of gpx-4 into PHGPx variants."
    Maiorino M., Scapin M., Ursini F., Biasolo M., Bosello V., Flohe L.
    Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: WistarImported.
  2. "Cloning of cDNA of rat phospholipid hydroperoxide/sperm nucleus glutathione peroxidase."
    Tramer F., Vetere A., Martinelli M., Paroni F., Marsich E., Sandri G., Panfili E.
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
  3. "Identification of a specific sperm nuclei selenoenzyme necessary for protamine thiol cross-linking during sperm maturation."
    Pfeifer H., Conrad M., Roethlein D., Kyriakopoulos A., Brielmeier M., Bornkamm G.W., Behne D.
    FASEB J. 15:1236-1238(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-250, SUBCELLULAR LOCATION.
    Tissue: TestisImported.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-78 AND SER-96, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGPX42_RAT
AccessioniPrimary (citable) accession number: Q91XR8
Secondary accession number(s): Q6PZ55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 23, 2003
Last sequence update: February 26, 2008
Last modified: May 11, 2016
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.