Q91XJ1 (BECN1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beclin-1

Alternative name(s):
Coiled-coil myosin-like BCL2-interacting protein
Protein GT197

Gene names

Name:

Becn1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense By similarity.
Subunit structure	Interacts with BCL2 and BCL2L1 By similarity. Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy By similarity. Interacts with VMP1. Interacts with PIK3CB By similarity. Interacts with DAPK1 By similarity. Ref.3
Subcellular location	Cytoplasm. Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein By similarity. Note: Interaction with ATG14 promotes translocation to autophagosomes By similarity.
Post-translational modification	Phosphorylation at Thr-117 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy By similarity. Ubiquitinated. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes By similarity.
Sequence similarities	Belongs to the beclin family.

Ontologies

Keywords
Biological process	Antiviral defense Autophagy Cell cycle Cell division
Cellular component	Cytoplasm Golgi apparatus Membrane
Domain	Coiled coil
PTM	Phosphoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	autophagic vacuole assembly Inferred from mutant phenotype PubMed 16420522. Source: RGD beta-amyloid metabolic process Inferred from electronic annotation. Source: Compara cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cellular response to aluminum ion Inferred from expression pattern PubMed 22138567. Source: RGD cellular response to epidermal growth factor stimulus Inferred from expression pattern PubMed 20539009. Source: RGD cytokinesis Inferred from sequence or structural similarity. Source: UniProtKB defense response to virus Inferred from electronic annotation. Source: UniProtKB-KW lysosome organization Inferred from electronic annotation. Source: Compara negative regulation of cell death Inferred from mutant phenotype PubMed 21490676. Source: RGD negative regulation of cell proliferation Inferred from electronic annotation. Source: Compara neuron development Inferred from electronic annotation. Source: Compara positive regulation of macroautophagy Inferred from electronic annotation. Source: Compara response to drug Inferred from expression pattern PubMed 22521819. Source: RGD response to hypoxia Inferred from expression pattern PubMed 22449108. Source: RGD response to vitamin E Inferred from expression pattern PubMed 19138675. Source: RGD
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from direct assay PubMed 21490676. Source: RGD cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: Compara dendrite Inferred from direct assay PubMed 21490676. Source: RGD membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from direct assay PubMed 21478185. Source: RGD protein complex Inferred from electronic annotation. Source: Compara trans-Golgi network Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 448

448

Beclin-1

PRO_0000316290

Regions

Coiled coil

140 – 267

128

Potential

Motif

106 – 125

20

BH3 By similarity

Amino acid modifications

Modified residue

117

1

Phosphothreonine; by DAPK1 By similarity

Secondary structure

1

.

.

.

448

Helix Strand Turn

Details...

Helix

174 – 263

90

Sequences

	Sequence		Length	Mass (Da)
	Q91XJ1 [UniParc]. Last modified December 1, 2001. Version 1. Checksum: 34981BE560F3354B	FASTA	448	51,557
	10 20 30 40 50 60 MEGSKASSST MQVSFVCQRC SQPLKLDTSF KILDRVTIQE LTAPLLTTAQ AKPGESQEEE 70 80 90 100 110 120 ANSGEEPFIE TRQDGVSRRF IPPARMMSTE SANSFTLIGE ASDGGTMENL SRRLKVTGDL 130 140 150 160 170 180 FDIMSGQTDV DHPLCEECTD TLLDQLDTQL NVTENECQNY KRCLEMLEQM NEGDSEQLQR 190 200 210 220 230 240 ELKELALEEE RLIQELEDVE KNRKVVAENL EKVQAEAERL DQEEAQYQRE YSEFKRQQLE 250 260 270 280 290 300 LDDELKSVEN QMRYAQMQLD KLKKTNVFNA TFHIWHSGQF GTINNFRLGR LPSAPVEWNE 310 320 330 340 350 360 INAAWGQTVL LLHALANKMG LKFQRYRLVP YGNHSYLESL TDKSKELPLY CSGGLRFFWD 370 380 390 400 410 420 NKFDHAMVAF LDCVQQFKEE VEKGETRFCL PYRMDVEKGK IEDTGGSGGS YSIKTQFNSE 430 440 EQWTKALKFM LTNLKWGLAW VSSQFYNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Beclin mRNA in the developing rat ovary." Paredes A.H., Tapia V., Ojeda S.R. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Ovary.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[3]	"Reduced expression of vacuole membrane protein 1 affects the invasion capacity of tumor cells." Sauermann M., Sahin O., Sultmann H., Hahne F., Blaszkiewicz S., Majety M., Zatloukal K., Fuzesi L., Poustka A., Wiemann S., Arlt D. Oncogene 27:1320-1326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH VMP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY033824 mRNA. Translation: AAK56548.1.
BC074011 mRNA. Translation: AAH74011.1.

IPI

IPI00325272.

RefSeq

NP_001029289.1. NM_001034117.1.
NP_446191.1. NM_053739.2.

UniGene

Rn.2776.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3Q8T	X-ray	1.90	A/B	174-264	[»]

ProteinModelPortal

Q91XJ1.

ModBase

Search...

Protein-protein interaction databases

STRING

10116.ENSRNOP00000027868.

Proteomic databases

PaxDb

Q91XJ1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000027868; ENSRNOP00000027868; ENSRNOG00000020513.

GeneID

114558.

KEGG

rno:114558.

UCSC

RGD:620190. rat.

Organism-specific databases

CTD

8678.

RGD

620190. Becn1.

Phylogenomic databases

eggNOG

NOG285533.

GeneTree

ENSGT00390000008164.

HOGENOM

HOG000158093.

HOVERGEN

HBG003181.

InParanoid

Q91XJ1.

KO

K08334.

OMA

VDNQMRY.

OrthoDB

EOG4320Z3.

Gene expression databases

Genevestigator

Q91XJ1.

Family and domain databases

InterPro

IPR007243. Beclin_fam.
[Graphical view]

PANTHER

PTHR12768. PTHR12768. 1 hit.

Pfam

PF04111. APG6. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

618705.

Entry information

Entry name

BECN1_RAT

Accession

Primary (citable) accession number: Q91XJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	December 1, 2001
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program