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Protein

Calcium-responsive transcription coactivator

Gene

Ss18l1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP.3 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-responsive transcription coactivator
Alternative name(s):
SS18-like protein 1
Gene namesi
Name:Ss18l1
Synonyms:Crest
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi621847. Ss18l1.

Subcellular locationi

GO - Cellular componenti

  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • condensed nuclear chromosome, centromeric region Source: Ensembl
  • nBAF complex Source: Ensembl
  • nuclear body Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 401401Calcium-responsive transcription coactivatorPRO_0000391347Add
BLAST

Proteomic databases

PaxDbiQ91XJ0.
PRIDEiQ91XJ0.

Expressioni

Tissue specificityi

Brain (at protein level). Also found in the heart, liver, kidney and testis.1 Publication

Developmental stagei

Detected as early as E18 in the developing cerebral cortex, and expression reaches its peak at P1, declines after P10, and remains at a relatively low level throughout adulthood. At E18, expressed in the forebrain, midbrain, and hindbrain. Within the forebrain, it is expressed in postmitotic cells of the cortical plate. At this stage, it can also be detected in other structures of central and peripheral nervous systems, including the trigeminal ganglion, superior cervical ganglion, retina and olfactory epithelium. Expression in the cortex is high at birth and declines substantially by P20. At P7, expressed in all cortical layers, and in the hippocampus, expression is high in the cell body layers of all subdivisions. Within the brain, expression decreases through development but continues to be expressed at high levels in the olfactory bulb, hippocampus, and cerebellum into adulthood.1 Publication

Gene expression databases

GenevisibleiQ91XJ0. RN.

Interactioni

Subunit structurei

Homodimer. Dimerization may be necessary for its function in neuronal dendritic development. Interacts (via C-terminus) with CREBBP (via N-terminus), EP300 and SMARCA4/BRG1. Interacts with the nBAF complex (By similarity). Association with CREBBP facilitates transcription while the association with SMARCA4/BRG1 suppresses CREST-mediated transcription in resting neurons.By similarity3 Publications

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011306.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 148148N-terminal auto-inhibitory domain; necessary for interaction with SMARCA4/BRG1Add
BLAST
Regioni149 – 23789Methionine-rich intra-molecular domainAdd
BLAST
Regioni251 – 32272MFD domainAdd
BLAST
Regioni339 – 40163Necessary for nuclear localizationAdd
BLAST
Regioni392 – 40110Necessary for interaction with CREBBP and for the recruitment of CREBBP to the nuclear bodies

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 534SH2-bindingSequence analysis
Motifi358 – 3614SH2-bindingSequence analysis
Motifi376 – 3849SH3-bindingSequence analysis
Motifi396 – 3994SH2-bindingSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi244 – 401158Gln-richAdd
BLAST

Domaini

The MFD (multi-functional domain) domain is involved in transcription transactivation, nuclear body targeting and dimerization. Inhibits dendritic growth in cultured neurons.

Sequence similaritiesi

Belongs to the SS18 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3227. Eukaryota.
ENOG41128HA. LUCA.
GeneTreeiENSGT00500000044808.
HOGENOMiHOG000048717.
HOVERGENiHBG003892.
InParanoidiQ91XJ0.
OMAiSMSITGP.
OrthoDBiEOG7B31R1.

Family and domain databases

InterProiIPR007726. SS18_fam.
[Graphical view]
PANTHERiPTHR23107. PTHR23107. 1 hit.
PfamiPF05030. SSXT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91XJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVAFASARP RGKGEVTQQT IQKMLDENHH LIQCILDYQS KGKTAECTQY
60 70 80 90 100
QQILHRNLVY LATIADSNQN MQSLLPAPPT QNMNLGPGAL SQSGSSQGLH
110 120 130 140 150
PQGSLSDTVS TGLPPASLMQ GQIGNGPNHV SMQQTAQSTL PTTSMSMSGS
160 170 180 190 200
GHGTGPGYSH SGPTSQSVPM QGQGAISNYV SRTNINMQSN PVSMMHQQAA
210 220 230 240 250
RPTTTQRRVE ASITRARRPL HDGPGWQGGS MMGQRPMAPY RPSQQGSSQQ
260 270 280 290 300
YLGQEEYYSE QYSHSQGSAE PMSQQYYPDG HSDYAYQQSS YTEQSYDRSF
310 320 330 340 350
EDPTQHYYEG GNSQYSQQQA GYQQGTAQQQ TYSQQQYPNQ QSYPGQQQGY
360 370 380 390 400
GPAQGAPSQY SGYQQGQGQQ YGSYRTSQTG PSAQQQRPYG YEQGQYGNYQ

Q
Length:401
Mass (Da):43,999
Last modified:December 1, 2001 - v1
Checksum:i2B1805EC74EEC12F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY034073 mRNA. Translation: AAK53461.1.
RefSeqiNP_620273.1. NM_138918.1.
UniGeneiRn.81037.

Genome annotation databases

EnsembliENSRNOT00000085718; ENSRNOP00000072296; ENSRNOG00000060010.
GeneIDi192352.
KEGGirno:192352.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY034073 mRNA. Translation: AAK53461.1.
RefSeqiNP_620273.1. NM_138918.1.
UniGeneiRn.81037.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011306.

Proteomic databases

PaxDbiQ91XJ0.
PRIDEiQ91XJ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000085718; ENSRNOP00000072296; ENSRNOG00000060010.
GeneIDi192352.
KEGGirno:192352.

Organism-specific databases

CTDi26039.
RGDi621847. Ss18l1.

Phylogenomic databases

eggNOGiKOG3227. Eukaryota.
ENOG41128HA. LUCA.
GeneTreeiENSGT00500000044808.
HOGENOMiHOG000048717.
HOVERGENiHBG003892.
InParanoidiQ91XJ0.
OMAiSMSITGP.
OrthoDBiEOG7B31R1.

Miscellaneous databases

PROiQ91XJ0.

Gene expression databases

GenevisibleiQ91XJ0. RN.

Family and domain databases

InterProiIPR007726. SS18_fam.
[Graphical view]
PANTHERiPTHR23107. PTHR23107. 1 hit.
PfamiPF05030. SSXT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Dendrite development regulated by CREST, a calcium-regulated transcriptional activator."
    Aizawa H., Hu S.-C., Bobb K., Balakrishnan K., Ince G., Gurevich I., Cowan M., Ghosh A.
    Science 303:197-202(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CREBBP AND EP300, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. "The calcium-responsive transactivator recruits CREB binding protein to nuclear bodies."
    Pradhan A., Liu Y.
    Neurosci. Lett. 370:191-195(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  3. "A multifunctional domain of the calcium-responsive transactivator (CREST) that inhibits dendritic growth in cultured neurons."
    Pradhan A., Liu Y.
    J. Biol. Chem. 280:24738-24743(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, MFD DOMAIN.
  4. "A calcium-dependent switch in a CREST-BRG1 complex regulates activity-dependent gene expression."
    Qiu Z., Ghosh A.
    Neuron 60:775-787(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH SMARCA4/BRG1.

Entry informationi

Entry nameiCREST_RAT
AccessioniPrimary (citable) accession number: Q91XJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: December 1, 2001
Last modified: July 6, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.