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Q91XF0

- PNPO_MOUSE

UniProt

Q91XF0 - PNPO_MOUSE

Protein

Pyridoxine-5'-phosphate oxidase

Gene

Pnpo

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP).By similarity

    Catalytic activityi

    Pyridoxamine 5'-phosphate + H2O + O2 = pyridoxal 5'-phosphate + NH3 + H2O2.
    Pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2.

    Cofactori

    Binds 1 FMN per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei95 – 951FMNBy similarity
    Binding sitei98 – 981FMN; via amide nitrogenBy similarity
    Binding sitei100 – 1001SubstrateBy similarity
    Binding sitei117 – 1171FMNBy similarity
    Binding sitei157 – 1571SubstrateBy similarity
    Binding sitei161 – 1611SubstrateBy similarity
    Binding sitei165 – 1651SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi110 – 1112FMNBy similarity
    Nucleotide bindingi174 – 1752FMNBy similarity

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. pyridoxamine-phosphate oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. pyridoxine biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridoxine biosynthesis

    Keywords - Ligandi

    Flavoprotein, FMN, Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_198547. Vitamins B6 activation to pyridoxal phosphate.
    UniPathwayiUPA00190; UER00304.
    UPA00190; UER00305.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyridoxine-5'-phosphate oxidase (EC:1.4.3.5)
    Alternative name(s):
    Pyridoxamine-phosphate oxidase
    Gene namesi
    Name:Pnpo
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:2144151. Pnpo.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 261261Pyridoxine-5'-phosphate oxidasePRO_0000167784Add
    BLAST

    Proteomic databases

    MaxQBiQ91XF0.
    PaxDbiQ91XF0.
    PRIDEiQ91XF0.

    PTM databases

    PhosphoSiteiQ91XF0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ91XF0.
    BgeeiQ91XF0.
    CleanExiMM_PNPO.
    GenevestigatoriQ91XF0.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    MINTiMINT-1869143.
    STRINGi10090.ENSMUSP00000018803.

    Structurei

    3D structure databases

    ProteinModelPortaliQ91XF0.
    SMRiQ91XF0. Positions 49-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni225 – 2273Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0259.
    GeneTreeiENSGT00390000011219.
    HOGENOMiHOG000242755.
    HOVERGENiHBG045634.
    InParanoidiQ91XF0.
    KOiK00275.
    OMAiNMGSRKA.
    OrthoDBiEOG7B8S4P.
    PhylomeDBiQ91XF0.
    TreeFamiTF313411.

    Family and domain databases

    Gene3Di2.30.110.10. 1 hit.
    HAMAPiMF_01629. PdxH.
    InterProiIPR000659. Pyridox_Oxase.
    IPR019740. Pyridox_Oxase_CS.
    IPR011576. Pyridox_Oxase_FMN-bd.
    IPR019576. Pyridoxamine_oxidase_dimer_C.
    IPR012349. Split_barrel_FMN-bd.
    [Graphical view]
    PANTHERiPTHR10851. PTHR10851. 1 hit.
    PfamiPF10590. PNPOx_C. 1 hit.
    PF01243. Pyridox_oxidase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000190. Pyd_amn-ph_oxd. 1 hit.
    SUPFAMiSSF50475. SSF50475. 1 hit.
    TIGRFAMsiTIGR00558. pdxH. 1 hit.
    PROSITEiPS01064. PYRIDOX_OXIDASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q91XF0-1 [UniParc]FASTAAdd to Basket

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    MTCGLLSVTV TFRRPAKWTG YLRHLCCRGA VMDLGPMRKS YRGDREAFEE    50
    THLTSLDPMK QFASWFDEAV QCPDIGEANA MCVATCTRDG KPSARMLLLK 100
    GFGKDGFRFF TNYESRKGKE LDSNPFASLV FYWEPLNRQV RVEGPVKKLP 150
    EKEAENYFHS RPKSSQIGAV VSRQSSVIPD REYLRKKNEE LGQLYQDQEV 200
    PKPEYWGGYI LYPQVMEFWQ GQTNRLHDRI VFRRGLATGD SPLGPMTHHG 250
    EEDWVYERLA P 261
    Length:261
    Mass (Da):30,114
    Last modified:December 1, 2001 - v1
    Checksum:iD83A54883B4BC0EC
    GO

    Sequence cautioni

    The sequence BAE32590.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK154004 mRNA. Translation: BAE32309.1.
    AK154443 mRNA. Translation: BAE32590.1. Different initiation.
    AK164667 mRNA. Translation: BAE37867.1.
    AL596384 Genomic DNA. Translation: CAM21902.1.
    BC010785 mRNA. Translation: AAH10785.1.
    BC026564 mRNA. Translation: AAH26564.1.
    CCDSiCCDS25308.1.
    RefSeqiNP_598782.1. NM_134021.2.
    UniGeneiMm.254704.

    Genome annotation databases

    EnsembliENSMUST00000018803; ENSMUSP00000018803; ENSMUSG00000018659.
    GeneIDi103711.
    KEGGimmu:103711.
    UCSCiuc007ldc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK154004 mRNA. Translation: BAE32309.1 .
    AK154443 mRNA. Translation: BAE32590.1 . Different initiation.
    AK164667 mRNA. Translation: BAE37867.1 .
    AL596384 Genomic DNA. Translation: CAM21902.1 .
    BC010785 mRNA. Translation: AAH10785.1 .
    BC026564 mRNA. Translation: AAH26564.1 .
    CCDSi CCDS25308.1.
    RefSeqi NP_598782.1. NM_134021.2.
    UniGenei Mm.254704.

    3D structure databases

    ProteinModelPortali Q91XF0.
    SMRi Q91XF0. Positions 49-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1869143.
    STRINGi 10090.ENSMUSP00000018803.

    PTM databases

    PhosphoSitei Q91XF0.

    Proteomic databases

    MaxQBi Q91XF0.
    PaxDbi Q91XF0.
    PRIDEi Q91XF0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000018803 ; ENSMUSP00000018803 ; ENSMUSG00000018659 .
    GeneIDi 103711.
    KEGGi mmu:103711.
    UCSCi uc007ldc.1. mouse.

    Organism-specific databases

    CTDi 55163.
    MGIi MGI:2144151. Pnpo.

    Phylogenomic databases

    eggNOGi COG0259.
    GeneTreei ENSGT00390000011219.
    HOGENOMi HOG000242755.
    HOVERGENi HBG045634.
    InParanoidi Q91XF0.
    KOi K00275.
    OMAi NMGSRKA.
    OrthoDBi EOG7B8S4P.
    PhylomeDBi Q91XF0.
    TreeFami TF313411.

    Enzyme and pathway databases

    UniPathwayi UPA00190 ; UER00304 .
    UPA00190 ; UER00305 .
    Reactomei REACT_198547. Vitamins B6 activation to pyridoxal phosphate.

    Miscellaneous databases

    NextBioi 356069.
    PROi Q91XF0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q91XF0.
    Bgeei Q91XF0.
    CleanExi MM_PNPO.
    Genevestigatori Q91XF0.

    Family and domain databases

    Gene3Di 2.30.110.10. 1 hit.
    HAMAPi MF_01629. PdxH.
    InterProi IPR000659. Pyridox_Oxase.
    IPR019740. Pyridox_Oxase_CS.
    IPR011576. Pyridox_Oxase_FMN-bd.
    IPR019576. Pyridoxamine_oxidase_dimer_C.
    IPR012349. Split_barrel_FMN-bd.
    [Graphical view ]
    PANTHERi PTHR10851. PTHR10851. 1 hit.
    Pfami PF10590. PNPOx_C. 1 hit.
    PF01243. Pyridox_oxidase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000190. Pyd_amn-ph_oxd. 1 hit.
    SUPFAMi SSF50475. SSF50475. 1 hit.
    TIGRFAMsi TIGR00558. pdxH. 1 hit.
    PROSITEi PS01064. PYRIDOX_OXIDASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and NOD.
      Tissue: Stomach and Thymus.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney and Liver.
    4. Lubec G., Klug S.
      Submitted (MAR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 120-138, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Hippocampus.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiPNPO_MOUSE
    AccessioniPrimary (citable) accession number: Q91XF0
    Secondary accession number(s): Q3TP70, Q3U445, Q3U4X1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2003
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3