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Protein

Vacuolar protein-sorting-associated protein 36

Gene

Vps36

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transcription, Transcription regulation, Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiREACT_334712. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein-sorting-associated protein 36
Alternative name(s):
ESCRT-II complex subunit VPS36
Gene namesi
Name:Vps36
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1917410. Vps36.

Subcellular locationi

GO - Cellular componenti

  • endosome Source: MGI
  • extracellular exosome Source: MGI
  • late endosome Source: MGI
  • lysosome Source: MGI
  • membrane Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Vacuolar protein-sorting-associated protein 36PRO_0000215223Add
BLAST

Proteomic databases

MaxQBiQ91XD6.
PaxDbiQ91XD6.
PRIDEiQ91XD6.

PTM databases

PhosphoSiteiQ91XD6.

Expressioni

Gene expression databases

BgeeiQ91XD6.
CleanExiMM_VPS36.
ExpressionAtlasiQ91XD6. baseline and differential.
GenevestigatoriQ91XD6.

Interactioni

Subunit structurei

Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 (By similarity). Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and two copies of VPS25 (By similarity). Interacts with VPS25, SNF8, TSG101 and CHMP6 (By similarity). Interacts (via GLUE domain) with ubiquitin (PubMed:15755741). Interacts with RILPL1 (via the C-terminal domain); which recruits ESCRT-II to the endosome membranes (By similarity). Interacts with ECM29 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi213894. 2 interactions.
DIPiDIP-29247N.
IntActiQ91XD6. 1 interaction.
MINTiMINT-4121697.

Structurei

Secondary structure

1
386
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi17 – 3014Combined sources
Beta strandi37 – 459Combined sources
Beta strandi47 – 537Combined sources
Helixi54 – 563Combined sources
Beta strandi63 – 653Combined sources
Beta strandi68 – 714Combined sources
Beta strandi82 – 854Combined sources
Beta strandi106 – 1138Combined sources
Turni115 – 1173Combined sources
Helixi118 – 12710Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DX5X-ray3.35A1-139[»]
ProteinModelPortaliQ91XD6.
SMRiQ91XD6. Positions 3-131, 172-386.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ91XD6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 8888GLUE N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini105 – 13834GLUE C-terminalPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili160 – 18526Sequence AnalysisAdd
BLAST

Domaini

The GLUE domain (GRAM-like ubiquitin-binding in EAP45) mediates binding to ubiquitin and phosphoinosides.1 Publication

Sequence similaritiesi

Belongs to the VPS36 family.Curated
Contains 1 GLUE C-terminal domain.PROSITE-ProRule annotation
Contains 1 GLUE N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG262969.
GeneTreeiENSGT00390000017209.
HOGENOMiHOG000006822.
HOVERGENiHBG083632.
InParanoidiQ91XD6.
KOiK12190.
OMAiFQSSKYS.
OrthoDBiEOG7VB2FM.
PhylomeDBiQ91XD6.
TreeFamiTF314770.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR007286. EAP30.
IPR021648. VPS36_GLUE.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04157. EAP30. 1 hit.
PF11605. Vps36_ESCRT-II. 1 hit.
[Graphical view]
PROSITEiPS51495. GLUE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91XD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRFVWTSGL LEINETLVIQ QRGVRVYDGE EKIKFDAGTL LLSTHRLIWR
60 70 80 90 100
DQKNNECCMA IPLSQIVFIE EQAAGIGKSA KIVVHLHPAP SNKEPGPFQS
110 120 130 140 150
SKNSYIRLSF KEHGQIEFYR RLSEEMTQRR WETVPVSQSL QTNKGPQPGR
160 170 180 190 200
VRAVGIVGIE RKLEEKRKET DKNISEAFED LSKLMIKAKE MVELSKSIAN
210 220 230 240 250
KIKEKQGDVT EDETIRFKSY LLSMGIANPV TRETYGSGTQ YHMQLAKQLA
260 270 280 290 300
GILQAPLEER GGIMSLTEVY CLVNRARGME LLSPEDLVNA CKMLEALKLP
310 320 330 340 350
IRLRVFDSGV MVIELQTHKE EEMVASALET VSERGSLTSE EFAKLVGMSV
360 370 380
LLAKERLLLA EKMGHLCRDD SVEGLRFYPN LFMTQN
Length:386
Mass (Da):43,736
Last modified:December 1, 2001 - v1
Checksum:i0036479499DD89C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC010811 mRNA. Translation: AAH10811.1.
AK008946 mRNA. Translation: BAB25984.1.
CCDSiCCDS22171.1.
RefSeqiNP_081614.1. NM_027338.1.
UniGeneiMm.41453.

Genome annotation databases

EnsembliENSMUST00000033866; ENSMUSP00000033866; ENSMUSG00000031479.
GeneIDi70160.
KEGGimmu:70160.
UCSCiuc009lct.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC010811 mRNA. Translation: AAH10811.1.
AK008946 mRNA. Translation: BAB25984.1.
CCDSiCCDS22171.1.
RefSeqiNP_081614.1. NM_027338.1.
UniGeneiMm.41453.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DX5X-ray3.35A1-139[»]
ProteinModelPortaliQ91XD6.
SMRiQ91XD6. Positions 3-131, 172-386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213894. 2 interactions.
DIPiDIP-29247N.
IntActiQ91XD6. 1 interaction.
MINTiMINT-4121697.

PTM databases

PhosphoSiteiQ91XD6.

Proteomic databases

MaxQBiQ91XD6.
PaxDbiQ91XD6.
PRIDEiQ91XD6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033866; ENSMUSP00000033866; ENSMUSG00000031479.
GeneIDi70160.
KEGGimmu:70160.
UCSCiuc009lct.1. mouse.

Organism-specific databases

CTDi51028.
MGIiMGI:1917410. Vps36.

Phylogenomic databases

eggNOGiNOG262969.
GeneTreeiENSGT00390000017209.
HOGENOMiHOG000006822.
HOVERGENiHBG083632.
InParanoidiQ91XD6.
KOiK12190.
OMAiFQSSKYS.
OrthoDBiEOG7VB2FM.
PhylomeDBiQ91XD6.
TreeFamiTF314770.

Enzyme and pathway databases

ReactomeiREACT_334712. Endosomal Sorting Complex Required For Transport (ESCRT).

Miscellaneous databases

ChiTaRSiVps36. mouse.
EvolutionaryTraceiQ91XD6.
NextBioi331106.
PROiQ91XD6.
SOURCEiSearch...

Gene expression databases

BgeeiQ91XD6.
CleanExiMM_VPS36.
ExpressionAtlasiQ91XD6. baseline and differential.
GenevestigatoriQ91XD6.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR007286. EAP30.
IPR021648. VPS36_GLUE.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF04157. EAP30. 1 hit.
PF11605. Vps36_ESCRT-II. 1 hit.
[Graphical view]
PROSITEiPS51495. GLUE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-161.
    Strain: C57BL/6J.
    Tissue: Stomach.
  3. "Eap45 in mammalian ESCRT-II binds ubiquitin via a phosphoinositide-interacting GLUE domain."
    Slagsvold T., Aasland R., Hirano S., Bache K.G., Raiborg C., Trambaiolo D., Wakatsuki S., Stenmark H.
    J. Biol. Chem. 280:19600-19606(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, LIPID-BINDING, INTERACTION WITH UBIQUITIN.
  4. Cited for: X-RAY CRYSTALLOGRAPHY (3.35 ANGSTROMS) OF 1-139 IN COMPLEX WITH RPS27A.

Entry informationi

Entry nameiVPS36_MOUSE
AccessioniPrimary (citable) accession number: Q91XD6
Secondary accession number(s): Q9CVA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 1, 2001
Last modified: May 27, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.