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Q91XD6 (VPS36_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar protein-sorting-associated protein 36
Alternative name(s):
ESCRT-II complex subunit VPS36
Gene names
Name:Vps36
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length386 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Its ability to bind ubiquitin probably plays a role in endosomal sorting of ubiquitinated cargo proteins by ESCRT complexes. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. Binds phosphoinosides such as PtdIns(3,4,5)P3 By similarity.

Subunit structure

Component of a complex at least composed of ELL, SNF8/EAP30, VPS25/EAP20 and VPS36/EAP45 By similarity. Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS36 and two copies of VPS25. Interacts with VPS25, SNF8, TSG101 and VPS36 By similarity. Interacts (via GLUE domain) with ubiquitin. Interacts with RILPL1 (via the C-terminal domain); which recruits ESCRT-II to the endosome membranes By similarity. Interacts with ECM29 By similarity.

Subcellular location

Cytoplasm By similarity. Endosome By similarity. Late endosome By similarity. Membrane By similarity. Nucleus By similarity. Note: Colocalizes with ubiquitinated proteins on late endosomes By similarity. Recruited to the endosome membrane to participate in vesicle formation By similarity.

Domain

The GLUE domain (GRAM-like ubiquitin-binding in EAP45) mediates the binding to ubiquitin and phosphoinosides By similarity.

Sequence similarities

Belongs to the VPS36 family.

Contains 1 GLUE C-terminal domain.

Contains 1 GLUE N-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 386386Vacuolar protein-sorting-associated protein 36
PRO_0000215223

Regions

Domain1 – 8888GLUE N-terminal
Domain105 – 13834GLUE C-terminal
Coiled coil160 – 18526 Potential

Secondary structure

................... 386
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q91XD6 [UniParc].

Last modified December 1, 2001. Version 1.
Checksum: 0036479499DD89C8

FASTA38643,736
        10         20         30         40         50         60 
MDRFVWTSGL LEINETLVIQ QRGVRVYDGE EKIKFDAGTL LLSTHRLIWR DQKNNECCMA 

        70         80         90        100        110        120 
IPLSQIVFIE EQAAGIGKSA KIVVHLHPAP SNKEPGPFQS SKNSYIRLSF KEHGQIEFYR 

       130        140        150        160        170        180 
RLSEEMTQRR WETVPVSQSL QTNKGPQPGR VRAVGIVGIE RKLEEKRKET DKNISEAFED 

       190        200        210        220        230        240 
LSKLMIKAKE MVELSKSIAN KIKEKQGDVT EDETIRFKSY LLSMGIANPV TRETYGSGTQ 

       250        260        270        280        290        300 
YHMQLAKQLA GILQAPLEER GGIMSLTEVY CLVNRARGME LLSPEDLVNA CKMLEALKLP 

       310        320        330        340        350        360 
IRLRVFDSGV MVIELQTHKE EEMVASALET VSERGSLTSE EFAKLVGMSV LLAKERLLLA 

       370        380 
EKMGHLCRDD SVEGLRFYPN LFMTQN 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-161.
Strain: C57BL/6J.
Tissue: Stomach.
[3]"Structural basis of ubiquitin recognition by mammalian Eap45 GLUE domain."
Hirano S., Suzuki N., Slagsvold T., Kawasaki M., Trambaiolo D., Kato R., Stenmark H., Wakatsuki S.
Nat. Struct. Mol. Biol. 13:1031-1032(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.35 ANGSTROMS) OF 1-139 IN COMPLEX WITH RPS27A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC010811 mRNA. Translation: AAH10811.1.
AK008946 mRNA. Translation: BAB25984.1.
CCDSCCDS22171.1.
RefSeqNP_081614.1. NM_027338.1.
UniGeneMm.41453.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DX5X-ray3.35A1-139[»]
ProteinModelPortalQ91XD6.
SMRQ91XD6. Positions 3-131, 172-386.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid213894. 2 interactions.
DIPDIP-29247N.
IntActQ91XD6. 1 interaction.
MINTMINT-4121697.

PTM databases

PhosphoSiteQ91XD6.

Proteomic databases

MaxQBQ91XD6.
PaxDbQ91XD6.
PRIDEQ91XD6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033866; ENSMUSP00000033866; ENSMUSG00000031479.
GeneID70160.
KEGGmmu:70160.
UCSCuc009lct.1. mouse.

Organism-specific databases

CTD51028.
MGIMGI:1917410. Vps36.

Phylogenomic databases

eggNOGNOG262969.
GeneTreeENSGT00390000017209.
HOGENOMHOG000006822.
HOVERGENHBG083632.
InParanoidQ91XD6.
KOK12190.
OMACCIAIPL.
OrthoDBEOG7VB2FM.
PhylomeDBQ91XD6.
TreeFamTF314770.

Gene expression databases

ArrayExpressQ91XD6.
BgeeQ91XD6.
CleanExMM_VPS36.
GenevestigatorQ91XD6.

Family and domain databases

Gene3D1.10.10.10. 2 hits.
InterProIPR007286. EAP30.
IPR021648. VPS36_GLUE.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF04157. EAP30. 1 hit.
PF11605. Vps36_ESCRT-II. 1 hit.
[Graphical view]
PROSITEPS51495. GLUE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ91XD6.
NextBio331106.
PROQ91XD6.
SOURCESearch...

Entry information

Entry nameVPS36_MOUSE
AccessionPrimary (citable) accession number: Q91XD6
Secondary accession number(s): Q9CVA3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 1, 2001
Last modified: July 9, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot