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Protein

LIM and senescent cell antigen-like-containing domain protein 2

Gene

Lims2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors.By similarity

GO - Molecular functioni

GO - Biological processi

  • cell-cell junction organization Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of epithelial cell proliferation Source: MGI
  • negative regulation of hepatocyte proliferation Source: MGI
  • negative regulation of neural precursor cell proliferation Source: MGI
  • positive regulation of integrin-mediated signaling pathway Source: MGI
  • single organismal cell-cell adhesion Source: MGI
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
LIM and senescent cell antigen-like-containing domain protein 2
Alternative name(s):
Particularly interesting new Cys-His protein 2
Short name:
PINCH-2
Gene namesi
Name:Lims2
Synonyms:Pinch2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:2385067. Lims2.

Subcellular locationi

GO - Cellular componenti

  • focal adhesion Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341LIM and senescent cell antigen-like-containing domain protein 2PRO_0000266012Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei328 – 3281PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ91XD2.
MaxQBiQ91XD2.
PaxDbiQ91XD2.
PRIDEiQ91XD2.

PTM databases

PhosphoSiteiQ91XD2.

Expressioni

Tissue specificityi

Detected in heart, lung, kidney, liver, urinary bladder, fat, skin, skeletal muscle, uterus, large intestine and testis.1 Publication

Developmental stagei

Not detectable during early stages of embryogenesis. Detected at low levels at E14.5 and E15.5. Highly expressed at E17.5.

Gene expression databases

BgeeiQ91XD2.
CleanExiMM_LIMS2.
GenevisibleiQ91XD2. MM.

Interactioni

Subunit structurei

Interacts with integrin-linked protein kinase 1 (ILK) via the first LIM domain, and in competition with LIMS1. Part of the heterotrimeric IPP complex composed of integrin-linked kinase (ILK), LIMS1 or LIMS2, and PARVA (By similarity). Interacts with TGFB1I1.By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025254.

Structurei

3D structure databases

ProteinModelPortaliQ91XD2.
SMRiQ91XD2. Positions 11-73, 76-310.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 7462LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini76 – 13358LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST
Domaini138 – 19558LIM zinc-binding 3PROSITE-ProRule annotationAdd
BLAST
Domaini196 – 25560LIM zinc-binding 4PROSITE-ProRule annotationAdd
BLAST
Domaini256 – 31560LIM zinc-binding 5PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 5 LIM zinc-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG2272. Eukaryota.
ENOG410XP46. LUCA.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000253950.
HOVERGENiHBG000053.
InParanoidiQ91XD2.
OMAiCFSCSFC.
OrthoDBiEOG7GBFX3.
PhylomeDBiQ91XD2.
TreeFamiTF314113.

Family and domain databases

Gene3Di2.10.110.10. 5 hits.
InterProiIPR017351. PINCH.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERiPTHR24210. PTHR24210. 1 hit.
PfamiPF00412. LIM. 5 hits.
[Graphical view]
PIRSFiPIRSF038003. PINCH. 1 hit.
SMARTiSM00132. LIM. 5 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 5 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q91XD2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGSNMSECL ADAMCQRCQA RFAPTERIVN SNGELYHEHC FVCAQCFRPF
60 70 80 90 100
PEGLFYEFEG RKYCEHDFQM LFAPCCGFCG EFVIGRVIKA MNANWHPGCF
110 120 130 140 150
RCELCDVELA DLGFVKNAGR HLCRPCHNRE KAKGLGKFIC QRCHLAIDEQ
160 170 180 190 200
PLMFKNDPYH PDHFSCSNCG KELTSDAREL KGELYCLPCH DKMGIPICGA
210 220 230 240 250
CRRPIEGRVV NALGKQWHVE HFVCAKCEKP FLGHRHYEKK GLAYCETHYN
260 270 280 290 300
QLFGDVCYNC SHVIEGDVVS ALSKAWCVNC FSCSTCNMKL TLKNKFVEFD
310 320 330 340
MKPVCKRCYE RFPLELKKRL KKLSDLSSRK AQPKSVDVNS L
Length:341
Mass (Da):39,031
Last modified:December 1, 2001 - v1
Checksum:i7A93F9845BDAED91
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ420860
, AJ420861, AJ420863, AJ420862 Genomic DNA. Translation: CAD12820.1.
BC010816 mRNA. Translation: AAH10816.1.
CCDSiCCDS29113.1.
RefSeqiNP_659111.1. NM_144862.3.
UniGeneiMm.24551.

Genome annotation databases

EnsembliENSMUST00000025254; ENSMUSP00000025254; ENSMUSG00000024395.
GeneIDi225341.
KEGGimmu:225341.
UCSCiuc008eiv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ420860
, AJ420861, AJ420863, AJ420862 Genomic DNA. Translation: CAD12820.1.
BC010816 mRNA. Translation: AAH10816.1.
CCDSiCCDS29113.1.
RefSeqiNP_659111.1. NM_144862.3.
UniGeneiMm.24551.

3D structure databases

ProteinModelPortaliQ91XD2.
SMRiQ91XD2. Positions 11-73, 76-310.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025254.

PTM databases

PhosphoSiteiQ91XD2.

Proteomic databases

EPDiQ91XD2.
MaxQBiQ91XD2.
PaxDbiQ91XD2.
PRIDEiQ91XD2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025254; ENSMUSP00000025254; ENSMUSG00000024395.
GeneIDi225341.
KEGGimmu:225341.
UCSCiuc008eiv.1. mouse.

Organism-specific databases

CTDi55679.
MGIiMGI:2385067. Lims2.

Phylogenomic databases

eggNOGiKOG2272. Eukaryota.
ENOG410XP46. LUCA.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000253950.
HOVERGENiHBG000053.
InParanoidiQ91XD2.
OMAiCFSCSFC.
OrthoDBiEOG7GBFX3.
PhylomeDBiQ91XD2.
TreeFamiTF314113.

Miscellaneous databases

ChiTaRSiLims2. mouse.
NextBioi377625.
PROiQ91XD2.
SOURCEiSearch...

Gene expression databases

BgeeiQ91XD2.
CleanExiMM_LIMS2.
GenevisibleiQ91XD2. MM.

Family and domain databases

Gene3Di2.10.110.10. 5 hits.
InterProiIPR017351. PINCH.
IPR001781. Znf_LIM.
[Graphical view]
PANTHERiPTHR24210. PTHR24210. 1 hit.
PfamiPF00412. LIM. 5 hits.
[Graphical view]
PIRSFiPIRSF038003. PINCH. 1 hit.
SMARTiSM00132. LIM. 5 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "PINCH2 is a new five LIM domain protein, homologous to PINCH and localized to focal adhesions."
    Braun A., Bordoy R., Stanchi F., Moser M., Kostka G., Ehler E., Brandau O., Faessler R.
    Exp. Cell Res. 284:239-250(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  3. "Oligomerizing potential of a focal adhesion LIM protein Hic-5 organizing a nuclear-cytoplasmic shuttling complex."
    Mori K., Asakawa M., Hayashi M., Imura M., Ohki T., Hirao E., Kim-Kaneyama J.-R., Nose K., Shibanuma M.
    J. Biol. Chem. 281:22048-22061(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TGFB1I1.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Lung.

Entry informationi

Entry nameiLIMS2_MOUSE
AccessioniPrimary (citable) accession number: Q91XD2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: December 1, 2001
Last modified: March 16, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.